Restriction map for
pF1KB4076
(Circular) (Six-base) MAPSORT of: pF1KB4076.seq Check: 3481 from: 1 to: 4832
pF1KB4076 4832 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 1870 1870
Size: 4832
Acc65I G'GTAC_C
Cuts at: 1848 1848
Size: 4832
AclI AA'CG_TT
Cuts at: 4672 4672
Size: 4832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 1080 1668 2657 3089 3836 4104 912
Size: 168 588 989 432 747 268 1640
Fragments arranged by size:
1640 989 747 588 432 268 168
AfeI AGC'GCT
Cuts at: 1766 1766
Size: 4832
AflIII A'CryG_T
Cuts at: 1156 2184 3288 4265 1156
Size: 1028 1104 977 1723
Fragments arranged by size:
1723 1104 1028 977
AgeI A'CCGG_T
Cuts at: 3220 3220
Size: 4832
AleI CACnn'nnGTG
Cuts at: 806 806
Size: 4832
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 244
Size: 32 4800
AlwNI CAG_nnn'CTG
Cuts at: 373 2137 3704 373
Size: 1764 1567 1501
Fragments arranged by size:
1764 1567 1501
ApaLI G'TGCA_C
Cuts at: 3602 3602
Size: 4832
ApoI r'AATT_y
Cuts at: 341 922 1382 1634 1836 4185 4441 341
Size: 581 460 252 202 2349 256 732
Fragments arranged by size:
2349 732 581 460 256 252 202
AseI AT'TA_AT
Cuts at: 20 20
Size: 4832
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
AvaI C'yCGr_G
Cuts at: 1852 1852
Size: 4832
BamHI G'GATC_C
Cuts at: 1857 1857
Size: 4832
BanI G'GyrC_C
Cuts at: 1848 2262 2510 2545 1848
Size: 414 248 35 4135
Fragments arranged by size:
4135 414 248 35
BanII G_rGCy'C
Cuts at: 323 1846 2876 323
Size: 1523 1030 2279
Fragments arranged by size:
2279 1523 1030
BbeI G_GCGC'C
Cuts at: 2266 2514 2266
Size: 248 4584
BbsI GAAGACnn'nnnn_
Cuts at: 619 619
Size: 4832
BciVI GTATCCnnnnn_n'
Cuts at: 1544 1786 2730 3491 4832 1544
Size: 242 944 761 1341 1544
Fragments arranged by size:
1544 1341 944 761 242
BclI T'GATC_A
Cuts at: 2352 2352
Size: 4832
BglII A'GATC_T
Cuts at: 1357 2347 4384 1357
Size: 990 2037 1805
Fragments arranged by size:
2037 1805 990
BlpI GC'TnA_GC
Cuts at: 209 638 1944 209
Size: 429 1306 3097
Fragments arranged by size:
3097 1306 429
Bme1580I G_kGCm'C
Cuts at: 2457 2550 3606 2457
Size: 93 1056 3683
Fragments arranged by size:
3683 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2044 2125 2460 4604 2044
Size: 81 335 2144 2272
Fragments arranged by size:
2272 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 612 3199 612
Size: 2587 2245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 992 1244 1496 1580 1748 259
Size: 733 252 252 84 168 3343
Fragments arranged by size:
3343 733 252 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2008 2887 3379 3677 2008
Size: 879 492 298 3163
Fragments arranged by size:
3163 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 346 500 1645 4536 346
Size: 154 1145 2891 642
Fragments arranged by size:
2891 1145 642 154
BsaAI yAC'GTr
Cuts at: 2185 2185
Size: 4832
BsaBI GATnn'nnATC
Cuts at: 6 2346 4383 6
Size: 2340 2037 455
Fragments arranged by size:
2340 2037 455
BsaHI Gr'CG_yC
Cuts at: 2263 2511 3213 4709 2263
Size: 248 702 1496 2386
Fragments arranged by size:
2386 1496 702 248
BsaWI w'CCGG_w
Cuts at: 299 1604 2021 2542 3220 3494 3641 4052 299
Size: 1305 417 521 678 274 147 411 1079
Fragments arranged by size:
1305 1079 678 521 417 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 199
Size: 4832
BseYI C'CCAG_C
Cuts at: 2257 3015 3592 4088 2257
Size: 758 577 496 3001
Fragments arranged by size:
3001 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2374 2420 3628 104
Size: 2270 46 1208 1308
Fragments arranged by size:
2270 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1276 1846 2624 2814 3606 1276
Size: 570 778 190 792 2502
Fragments arranged by size:
2502 792 778 570 190
BsmI GAATG_Cn'
Cuts at: 559 1330 1582 559
Size: 771 252 3809
Fragments arranged by size:
3809 771 252
Bsp1286I G_dGCh'C
Cuts at: 323 1276 1846 2457 2550 2624 2814 2876 3606
Size: 953 570 611 93 74 190 62 730
Cuts at: 3606 323
Size: 1549
Fragments arranged by size:
1549 953 730 611 570 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1604 4052 1604
Size: 2448 2384
BspHI T'CATG_A
Cuts at: 1497 4008 1497
Size: 2511 2321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1882 2096 2398 2779 1882
Size: 214 302 381 3935
Fragments arranged by size:
3935 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3124 3178 4662 4827 50
Size: 3074 54 1484 165 55
Fragments arranged by size:
3074 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1335 2744 1335
Size: 1409 3423
BsrFI r'CCGG_y
Cuts at: 2830 3011 3220 2830
Size: 181 209 4442
Fragments arranged by size:
4442 209 181
BsrGI T'GTAC_A
Cuts at: 221 1151 221
Size: 930 3902
BssSI C'ACGA_G
Cuts at: 3103 3461 3103
Size: 358 4474
BstAPI GCAn_nnn'nTGC
Cuts at: 2209 2209
Size: 4832
BstBI TT'CG_AA
Cuts at: 3193 3193
Size: 4832
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 4832
BstYI r'GATC_y
Cuts at: 1 1357 1857 2324 2347 2682 2928 3940 4026
Size: 1356 500 467 23 335 246 1012 86
Cuts at: 4026 4038 4384 1
Size: 12 346 449
Fragments arranged by size:
1356 1012 500 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4832
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2782 2963 3126 2782
Size: 181 163 4488
Fragments arranged by size:
4488 181 163
BtsI GCAGTG_nn'
Cuts at: 203 2075 203
Size: 1872 2960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1317 1352 1737 1772 1317
Size: 35 385 35 4377
Fragments arranged by size:
4377 385 35 35
DraI TTT'AAA
Cuts at: 417 692 1831 417
Size: 275 1139 3418
Fragments arranged by size:
3418 1139 275
DrdI GACnn_nn'nnGTC
Cuts at: 2538 3396 2538
Size: 858 3974
EaeI y'GGCC_r
Cuts at: 2417 2591 2982 3009 4354 2417
Size: 174 391 27 1345 2895
Fragments arranged by size:
2895 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2417 2417
Size: 4832
EarI CTCTTCn'nnn_
Cuts at: 311 1643 2855 3065 311
Size: 1332 1212 210 2078
Fragments arranged by size:
2078 1332 1212 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3040 3350 3496 4642 3040
Size: 310 146 1146 3230
Fragments arranged by size:
3230 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1844 1844
Size: 4832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 912 992 1080 1244 1496 1580 1668 1748
Size: 653 80 88 164 252 84 88 80
Cuts at: 1748 2657 3089 3836 4104 259
Size: 909 432 747 268 987
Fragments arranged by size:
987 909 747 653 432 268 252 164
88 88 84 80 80
EcoO109I rG'GnC_Cy
Cuts at: 1316 1971 1316
Size: 655 4177
EcoRI G'AATT_C
Cuts at: 1382 1634 1836 1382
Size: 252 202 4378
Fragments arranged by size:
4378 252 202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 4832
FspI TGC'GCA
Cuts at: 2613 2613
Size: 4832
HaeII r_GCGC'y
Cuts at: 939 1768 2266 2514 3536 4515 939
Size: 829 498 248 1022 979 1256
Fragments arranged by size:
1256 1022 979 829 498 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 412 444 1263 1295 2674 2704 2706
Size: 32 277 32 819 32 1379 30 2
Cuts at: 2706 2736 103
Size: 30 2199
Fragments arranged by size:
2199 1379 819 277 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1871 1871
Size: 4832
KasI G'GCGC_C
Cuts at: 2262 2510 2262
Size: 248 4584
KpnI G_GTAC'C
Cuts at: 1852 1852
Size: 4832
MluI A'CGCG_T
Cuts at: 4265 4265
Size: 4832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 1623 3503 3687 284
Size: 1339 1880 184 1429
Fragments arranged by size:
1880 1429 1339 184
MscI TGG'CCA
Cuts at: 2593 2593
Size: 4832
MslI CAynn'nnrTG
Cuts at: 442 668 806 1055 1491 1502 1575 2821 2948
Size: 226 138 249 436 11 73 1246 127
Cuts at: 2948 442
Size: 2326
Fragments arranged by size:
2326 1246 436 249 226 138 127 73
11
MspA1I CmG'CkG
Cuts at: 932 1100 1943 2257 2617 3630 3875 932
Size: 168 843 314 360 1013 245 1889
Fragments arranged by size:
1889 1013 843 360 314 245 168
NaeI GCC'GGC
Cuts at: 3013 3013
Size: 4832
NarI GG'CG_CC
Cuts at: 2263 2511 2263
Size: 248 4584
NcoI C'CATG_G
Cuts at: 107 107
Size: 4832
NdeI CA'TA_TG
Cuts at: 1488 1572 1488
Size: 84 4748
NgoMIV G'CCGG_C
Cuts at: 3011 3011
Size: 4832
NspI r_CATG'y
Cuts at: 1160 1748 1885 2385 3292 1160
Size: 588 137 500 907 2700
Fragments arranged by size:
2700 907 588 500 137
PciI A'CATG_T
Cuts at: 1156 3288 1156
Size: 2132 2700
PfoI T'CCnGG_A
Cuts at: 136 136
Size: 4832
PmeI GTTT'AAAC
Cuts at: 1831 1831
Size: 4832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4800
PstI C_TGCA'G
Cuts at: 1879 2564 1879
Size: 685 4147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4832
PvuII CAG'CTG
Cuts at: 932 1100 2257 2617 932
Size: 168 1157 360 3147
Fragments arranged by size:
3147 1157 360 168
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 4832
SacI G_AGCT'C
Cuts at: 1846 1846
Size: 4832
SalI G'TCGA_C
Cuts at: 1869 1869
Size: 4832
SapI GCTCTTCn'nnn_
Cuts at: 311 2855 3065 311
Size: 2544 210 2078
Fragments arranged by size:
2544 2078 210
SbfI CC_TGCA'GG
Cuts at: 1879 1879
Size: 4832
ScaI AGT'ACT
Cuts at: 578 647 4349 578
Size: 69 3702 1061
Fragments arranged by size:
3702 1061 69
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 4832
SfcI C'TryA_G
Cuts at: 33 1875 2560 3553 3744 33
Size: 1842 685 993 191 1121
Fragments arranged by size:
1842 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2264 2512 2264
Size: 248 4584
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
SmaI CCC'GGG
Cuts at: 1854 1854
Size: 4832
SmlI C'TyrA_G
Cuts at: 1987 2902 3394 3656 1987
Size: 915 492 262 3163
Fragments arranged by size:
3163 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4832
SphI G_CATG'C
Cuts at: 1885 2385 1885
Size: 500 4332
StyI C'CwwG_G
Cuts at: 107 263 1966 2320 107
Size: 156 1703 354 2619
Fragments arranged by size:
2619 1703 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 2415 2548 3007 3216 580
Size: 1835 133 459 209 2196
Fragments arranged by size:
2196 1835 459 209 133
TatI w'GTAC_w
Cuts at: 221 576 645 1151 4347 221
Size: 355 69 506 3196 706
Fragments arranged by size:
3196 706 506 355 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4349 4381 4349
Size: 32 4800
Tth111I GACn'n_nGTC
Cuts at: 2629 2629
Size: 4832
XbaI T'CTAG_A
Cuts at: 1863 1863
Size: 4832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2327
Size: 4832
XmaI C'CCGG_G
Cuts at: 1852 1852
Size: 4832
XmnI GAAnn'nnTTC
Cuts at: 1261 1597 1261
Size: 336 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI