Restriction map for
pF1KB4055
(Circular) (Six-base) MAPSORT of: pF1KB4055.seq Check: 1236 from: 1 to: 4112
pF1KB4055 4112 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 654 654
Size: 4112
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 877 1937 2369 3116 3384 788
Size: 89 1060 432 747 268 1516
Fragments arranged by size:
1516 1060 747 432 268 89
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AhdI GACnn_n'nnGTC
Cuts at: 695 695
Size: 4112
AleI CACnn'nnGTG
Cuts at: 841 841
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 387 387
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 213 229 433 454 481 1116 3465 3721 213
Size: 16 204 21 27 635 2349 256 604
Fragments arranged by size:
2349 635 604 256 204 27 21 16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 1043 1128 1542 1790 1825 1043
Size: 85 414 248 35 3330
Fragments arranged by size:
3330 414 248 85 35
BanII G_rGCy'C
Cuts at: 965 1084 1126 2156 965
Size: 119 42 1030 2921
Fragments arranged by size:
2921 1030 119 42
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BbsI GAAGACnn'nnnn_
Cuts at: 996 996
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BfrBI ATG'CAT
Cuts at: 812 812
Size: 4112
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 913 1224 913
Size: 311 3801
Bme1580I G_kGCm'C
Cuts at: 1046 1737 1830 2886 1046
Size: 691 93 1056 2272
Fragments arranged by size:
2272 1056 691 93
BmrI ACTGGGnnnn_n'
Cuts at: 959 1324 1405 1740 3884 959
Size: 365 81 335 2144 1187
Fragments arranged by size:
2144 1187 365 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
Bpu10I CC'TnA_GC
Cuts at: 985 985
Size: 4112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 2167 2659 2957 1288
Size: 879 492 298 2443
Fragments arranged by size:
2443 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 651 1543 1791 2493 3989 651
Size: 892 248 702 1496 774
Fragments arranged by size:
1496 892 774 702 248
BsaWI w'CCGG_w
Cuts at: 863 1301 1822 2500 2774 2921 3332 863
Size: 438 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 438 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 391 882 391
Size: 491 3621
BseYI C'CCAG_C
Cuts at: 143 1537 2295 2872 3368 143
Size: 1394 758 577 496 887
Fragments arranged by size:
1394 887 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 965 1046 1084 1126 1737 1830 1904 2094 2156
Size: 81 38 42 611 93 74 190 62
Cuts at: 2156 2886 965
Size: 730 2191
Fragments arranged by size:
2191 730 611 190 93 81 74 62
42 38
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 590 3288 590
Size: 2698 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 2110 2291 2500 2110
Size: 181 209 3722
Fragments arranged by size:
3722 209 181
BsrGI T'GTAC_A
Cuts at: 1052 1052
Size: 4112
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 616 799 1489 616
Size: 183 690 3239
Fragments arranged by size:
3239 690 183
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstYI r'GATC_y
Cuts at: 1 584 639 1137 1604 1627 1962 2208 3220
Size: 583 55 498 467 23 335 246 1012
Cuts at: 3220 3306 3318 3664 1
Size: 86 12 346 449
Fragments arranged by size:
1012 583 498 467 449 346 335 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
ClaI AT'CG_AT
Cuts at: 187 187
Size: 4112
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 1050 1050
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 906 1818 2676 906
Size: 912 858 2342
Fragments arranged by size:
2342 912 858
EaeI y'GGCC_r
Cuts at: 318 923 1697 1871 2262 2289 3634 318
Size: 605 774 174 391 27 1345 796
Fragments arranged by size:
1345 796 774 605 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 877 1937 2369 3116 3384 788
Size: 89 1060 432 747 268 1516
Fragments arranged by size:
1516 1060 747 432 268 89
EcoNI CCTnn'n_nnAGG
Cuts at: 242 242
Size: 4112
EcoO109I rG'GnC_Cy
Cuts at: 537 1251 537
Size: 714 3398
EcoRI G'AATT_C
Cuts at: 213 454 1116 213
Size: 241 662 3209
Fragments arranged by size:
3209 662 241
EcoRV GAT'ATC
Cuts at: 429 429
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 746 1546 1794 2816 3795 746
Size: 800 248 1022 979 1063
Fragments arranged by size:
1063 1022 979 800 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 681 713 800 832 1954 1984 1986 2016 681
Size: 32 87 32 1122 30 2 30 2777
Fragments arranged by size:
2777 1122 87 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 579 667 2783 2967 579
Size: 88 2116 184 1724
Fragments arranged by size:
2116 1724 184 88
MscI TGG'CCA
Cuts at: 320 925 1873 320
Size: 605 948 2559
Fragments arranged by size:
2559 948 605
MslI CAynn'nnrTG
Cuts at: 112 841 2101 2228 112
Size: 729 1260 127 1996
Fragments arranged by size:
1996 1260 729 127
MspA1I CmG'CkG
Cuts at: 941 1031 1223 1537 1897 2910 3155 941
Size: 90 192 314 360 1013 245 1898
Fragments arranged by size:
1898 1013 360 314 245 192 90
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NcoI C'CATG_G
Cuts at: 107 107
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NsiI A_TGCA'T
Cuts at: 814 814
Size: 4112
NspI r_CATG'y
Cuts at: 1165 1665 2572 1165
Size: 500 907 2705
Fragments arranged by size:
2705 907 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PfoI T'CCnGG_A
Cuts at: 1061 1061
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PsiI TTA'TAA
Cuts at: 335 335
Size: 4112
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1081 1113 1081
Size: 32 4080
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 139 3629 139
Size: 3490 622
SfcI C'TryA_G
Cuts at: 33 1155 1840 2833 3024 33
Size: 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 1267 2182 2674 2936 1267
Size: 915 492 262 2443
Fragments arranged by size:
2443 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
SspI AAT'ATT
Cuts at: 331 331
Size: 4112
StyI C'CwwG_G
Cuts at: 107 1246 1600 107
Size: 1139 354 2619
Fragments arranged by size:
2619 1139 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 945 1695 1828 2287 2496 945
Size: 750 133 459 209 2561
Fragments arranged by size:
2561 750 459 209 133
TatI w'GTAC_w
Cuts at: 137 1052 3627 137
Size: 915 2575 622
Fragments arranged by size:
2575 915 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 115 147 3629 3661 115
Size: 32 3482 32 566
Fragments arranged by size:
3482 566 32 32
Tth111I GACn'n_nGTC
Cuts at: 1000 1909 1000
Size: 909 3203
XbaI T'CTAG_A
Cuts at: 477 1143 477
Size: 666 3446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1607 1607
Size: 4112
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 684 684
Size: 4112
ZraI GAC'GTC
Cuts at: 652 652
Size: 4112
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AloI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI BpmI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI