Restriction map for
pF1KB4011
(Circular) (Six-base) MAPSORT of: pF1KB4011.seq Check: 2838 from: 1 to: 4766
pF1KB4011 4766 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 248 248
Size: 4766
AccI GT'mk_AC
Cuts at: 1804 1804
Size: 4766
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 430 535 757 851 931 1318 2591 3023
Size: 60 105 222 94 80 387 1273 432
Cuts at: 3023 3770 4038 370
Size: 747 268 1098
Fragments arranged by size:
1273 1098 747 432 387 268 222 105
94 80 60
AfeI AGC'GCT
Cuts at: 1213 1213
Size: 4766
AflIII A'CryG_T
Cuts at: 714 2118 3222 4199 714
Size: 1404 1104 977 1281
Fragments arranged by size:
1404 1281 1104 977
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AleI CACnn'nnGTG
Cuts at: 1343 1343
Size: 4766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 4766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1060 1092 1060
Size: 32 4734
AlwNI CAG_nnn'CTG
Cuts at: 1197 1356 2071 3638 1197
Size: 159 715 1567 2325
Fragments arranged by size:
2325 1567 715 159
ApaI G_GGCC'C
Cuts at: 143 143
Size: 4766
ApaLI G'TGCA_C
Cuts at: 261 1642 3536 261
Size: 1381 1894 1491
Fragments arranged by size:
1894 1491 1381
ApoI r'AATT_y
Cuts at: 1770 4119 4375 1770
Size: 2349 256 2161
Fragments arranged by size:
2349 2161 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 520 1786 520
Size: 1266 3500
BamHI G'GATC_C
Cuts at: 236 948 1791 236
Size: 712 843 3211
Fragments arranged by size:
3211 843 712
BanI G'GyrC_C
Cuts at: 883 1337 1443 1782 2196 2444 2479 883
Size: 454 106 339 414 248 35 3170
Fragments arranged by size:
3170 454 414 339 248 106 35
BanII G_rGCy'C
Cuts at: 143 230 422 631 1780 2810 143
Size: 87 192 209 1149 1030 2099
Fragments arranged by size:
2099 1149 1030 209 192 87
BbeI G_GCGC'C
Cuts at: 1447 2200 2448 1447
Size: 753 248 3765
Fragments arranged by size:
3765 753 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1104 1138 90
Size: 34 980 34 3718
Fragments arranged by size:
3718 980 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3425 4766 2664
Size: 761 1341 2664
Fragments arranged by size:
2664 1341 761
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglI GCCn_nnn'nGGC
Cuts at: 1183 1183
Size: 4766
BglII A'GATC_T
Cuts at: 2281 4318 2281
Size: 2037 2729
BlpI GC'TnA_GC
Cuts at: 497 1878 497
Size: 1381 3385
Bme1580I G_kGCm'C
Cuts at: 143 265 657 877 1406 1646 2391 2484 3540
Size: 122 392 220 529 240 745 93 1056
Cuts at: 3540 143
Size: 1369
Fragments arranged by size:
1369 1056 745 529 392 240 220 122
93
BmgBI CAC'GTC
Cuts at: 645 645
Size: 4766
BmrI ACTGGGnnnn_n'
Cuts at: 667 1978 2059 2394 4538 667
Size: 1311 81 335 2144 895
Fragments arranged by size:
2144 1311 895 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1636 211
Size: 1425 3341
Bpu10I CC'TnA_GC
Cuts at: 162 1264 162
Size: 1102 3664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 2821 3313 3611 1942
Size: 879 492 298 3097
Fragments arranged by size:
3097 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 112 4470 112
Size: 4358 408
BsaAI yAC'GTr
Cuts at: 717 2119 717
Size: 1402 3364
BsaBI GATnn'nnATC
Cuts at: 6 2280 4317 6
Size: 2274 2037 455
Fragments arranged by size:
2274 2037 455
BsaHI Gr'CG_yC
Cuts at: 679 1444 2197 2445 3147 4643 679
Size: 765 753 248 702 1496 802
Fragments arranged by size:
1496 802 765 753 702 248
BsaWI w'CCGG_w
Cuts at: 1955 2476 3154 3428 3575 3986 1955
Size: 521 678 274 147 411 2735
Fragments arranged by size:
2735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 217 247 184
Size: 30 3 30 4703
Fragments arranged by size:
4703 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 994 1108 1318 994
Size: 114 210 4442
Fragments arranged by size:
4442 210 114
BseYI C'CCAG_C
Cuts at: 273 1356 1593 2191 2949 3526 4022 273
Size: 1083 237 598 758 577 496 1017
Fragments arranged by size:
1083 1017 758 598 577 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 1546
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3562 104
Size: 2204 46 1208 1308
Fragments arranged by size:
2204 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 265 422 631 1385 1646 1780 2558 2748 3540
Size: 157 209 754 261 134 778 190 792
Cuts at: 3540 265
Size: 1491
Fragments arranged by size:
1491 792 778 754 261 209 190 157
134
Bsp1286I G_dGCh'C
Cuts at: 143 230 265 422 631 657 877 1385 1406
Size: 87 35 157 209 26 220 508 21
Cuts at: 1406 1646 1780 2391 2484 2558 2748 2810 3540
Size: 240 134 611 93 74 190 62 730
Cuts at: 3540 143
Size: 1369
Fragments arranged by size:
1369 730 611 508 240 220 209 190
157 134 93 87 74 62 35 26
21
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 3942 3942
Size: 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 1816 2030 2332 2713 248
Size: 1568 214 302 381 2301
Fragments arranged by size:
2301 1568 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 1749 3058 3112 4596 4761 50
Size: 111 1588 1309 54 1484 165 55
Fragments arranged by size:
1588 1484 1309 165 111 55 54
BsrDI GCAATG_nn'
Cuts at: 896 2678 896
Size: 1782 2984
BsrFI r'CCGG_y
Cuts at: 278 2764 2945 3154 278
Size: 2486 181 209 1890
Fragments arranged by size:
2486 1890 209 181
BssSI C'ACGA_G
Cuts at: 1645 3037 3395 1645
Size: 1392 358 3016
Fragments arranged by size:
3016 1392 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1197 2143 1197
Size: 946 3820
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstEII G'GTnAC_C
Cuts at: 1707 1707
Size: 4766
BstXI CCAn_nnnn'nTGG
Cuts at: 1050 1050
Size: 4766
BstYI r'GATC_y
Cuts at: 1 236 824 948 1415 1574 1652 1791 2258
Size: 235 588 124 467 159 78 139 467
Cuts at: 2258 2281 2616 2862 3874 3960 3972 4318 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 588 467 467 449 346 335 246
235 159 139 124 86 78 23 12
BtgI C'CryG_G
Cuts at: 1043 1448 1043
Size: 405 4361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 308 590 841 1158 2009 308
Size: 282 251 317 851 3065
Fragments arranged by size:
3065 851 317 282 251
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3330 2472
Size: 858 3908
EaeI y'GGCC_r
Cuts at: 425 1175 1736 2351 2525 2916 2943 4288 425
Size: 750 561 615 174 391 27 1345 903
Fragments arranged by size:
1345 903 750 615 561 391 174 27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1567 2974 3284 3430 4576 1567
Size: 1407 310 146 1146 1757
Fragments arranged by size:
1757 1407 1146 310 146
EcoICRI GAG'CTC
Cuts at: 420 629 1778 420
Size: 209 1149 3408
Fragments arranged by size:
3408 1149 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 370 430 535 757 851 931 1318 1636
Size: 159 60 105 222 94 80 387 318
Cuts at: 1636 2591 3023 3770 4038 211
Size: 955 432 747 268 939
Fragments arranged by size:
955 939 747 432 387 318 268 222
159 105 94 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 193 193
Size: 4766
EcoO109I rG'GnC_Cy
Cuts at: 139 200 1308 1905 139
Size: 61 1108 597 3000
Fragments arranged by size:
3000 1108 597 61
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4766
FseI GG_CCGG'CC
Cuts at: 282 282
Size: 4766
FspI TGC'GCA
Cuts at: 1190 2547 1190
Size: 1357 3409
HaeII r_GCGC'y
Cuts at: 1215 1447 2200 2448 3470 4449 1215
Size: 232 753 248 1022 979 1532
Fragments arranged by size:
1532 1022 979 753 248 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 529 561 579 611 920 952 2608
Size: 32 313 32 18 32 309 32 1656
Cuts at: 2608 2638 2640 2670 184
Size: 30 2 30 2280
Fragments arranged by size:
2280 1656 313 309 32 32 32 32
30 30 18 2
HincII GTy'rAC
Cuts at: 1805 1805
Size: 4766
KasI G'GCGC_C
Cuts at: 1443 2196 2444 1443
Size: 753 248 3765
Fragments arranged by size:
3765 753 248
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3621 3437
Size: 184 4582
MscI TGG'CCA
Cuts at: 427 2527 427
Size: 2100 2666
MslI CAynn'nnrTG
Cuts at: 370 607 610 859 943 1343 2755 2882 370
Size: 237 3 249 84 400 1412 127 2254
Fragments arranged by size:
2254 1412 400 249 237 127 84 3
MspA1I CmG'CkG
Cuts at: 273 817 1374 1686 1877 2191 2551 3564 3809
Size: 544 557 312 191 314 360 1013 245
Cuts at: 3809 273
Size: 1230
Fragments arranged by size:
1230 1013 557 544 360 314 312 245
191
NaeI GCC'GGC
Cuts at: 280 2947 280
Size: 2667 2099
NarI GG'CG_CC
Cuts at: 1444 2197 2445 1444
Size: 753 248 3765
Fragments arranged by size:
3765 753 248
NgoMIV G'CCGG_C
Cuts at: 278 2945 278
Size: 2667 2099
NspI r_CATG'y
Cuts at: 858 1119 1500 1819 2319 3226 858
Size: 261 381 319 500 907 2398
Fragments arranged by size:
2398 907 500 381 319 261
PciI A'CATG_T
Cuts at: 3222 3222
Size: 4766
PfoI T'CCnGG_A
Cuts at: 547 924 1421 1613 547
Size: 377 497 192 3700
Fragments arranged by size:
3700 497 377 192
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PmlI CAC'GTG
Cuts at: 717 717
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1084 1116 1486 1518 3905 3937 1084
Size: 32 370 32 2387 32 1913
Fragments arranged by size:
2387 1913 370 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 200 1308 200
Size: 1108 3658
PspOMI G'GGCC_C
Cuts at: 139 139
Size: 4766
PstI C_TGCA'G
Cuts at: 833 1202 1286 1813 2498 833
Size: 369 84 527 685 3101
Fragments arranged by size:
3101 685 527 369 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 273 2191 2551 273
Size: 1918 360 2488
Fragments arranged by size:
2488 1918 360
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 422 631 1780 422
Size: 209 1149 3408
Fragments arranged by size:
3408 1149 209
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SanDI GG'GwC_CC
Cuts at: 200 200
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SfcI C'TryA_G
Cuts at: 33 829 1198 1282 1809 2494 3487 3678 33
Size: 796 369 84 527 685 993 191 1121
Fragments arranged by size:
1121 993 796 685 527 369 191 84
SfoI GGC'GCC
Cuts at: 1445 2198 2446 1445
Size: 753 248 3765
Fragments arranged by size:
3765 753 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 522 1788 522
Size: 1266 3500
SmlI C'TyrA_G
Cuts at: 1921 2836 3328 3590 1921
Size: 915 492 262 3097
Fragments arranged by size:
3097 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1119 1819 2319 1119
Size: 700 500 3566
Fragments arranged by size:
3566 700 500
StyI C'CwwG_G
Cuts at: 221 1900 2254 221
Size: 1679 354 2733
Fragments arranged by size:
2733 1679 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2349 2482 2941 3150 2349
Size: 133 459 209 3965
Fragments arranged by size:
3965 459 209 133
TatI w'GTAC_w
Cuts at: 4281 4281
Size: 4766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 217 249 4283 4315 217
Size: 32 4034 32 668
Fragments arranged by size:
4034 668 32 32
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 792 2261 792
Size: 1469 3297
XmaI C'CCGG_G
Cuts at: 520 1786 520
Size: 1266 3500
XmnI GAAnn'nnTTC
Cuts at: 400 1432 400
Size: 1032 3734
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI