Restriction map for
pF1KB3946
(Circular) (Six-base) MAPSORT of: pF1KB3946.seq Check: 8585 from: 1 to: 4640
pF1KB3946 4640 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 953 953
Size: 4640
AccI GT'mk_AC
Cuts at: 1613 1678 1613
Size: 65 4575
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 883 2465 2897 3250 3912 307
Size: 576 1582 432 353 662 1035
Fragments arranged by size:
1582 1035 662 576 432 353
AflIII A'CryG_T
Cuts at: 260 1106 1992 3792 4073 260
Size: 846 886 1800 281 827
Fragments arranged by size:
1800 886 846 827 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 247 279 295 327 1438 1470 247
Size: 32 16 32 1111 32 3417
Fragments arranged by size:
3417 1111 32 32 32 16
AlwNI CAG_nnn'CTG
Cuts at: 999 1945 3383 999
Size: 946 1438 2256
Fragments arranged by size:
2256 1438 946
ApaI G_GGCC'C
Cuts at: 808 808
Size: 4640
ApaLI G'TGCA_C
Cuts at: 769 3478 769
Size: 2709 1931
ApoI r'AATT_y
Cuts at: 1644 3993 4249 1644
Size: 2349 256 2035
Fragments arranged by size:
2349 2035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 789 1017 1660 789
Size: 228 643 3769
Fragments arranged by size:
3769 643 228
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 166 372 875 1023 1128 1179 1200 1221 1483
Size: 206 503 148 105 51 21 21 262
Cuts at: 1483 1656 2070 2318 2353 166
Size: 173 414 248 35 2453
Fragments arranged by size:
2453 503 414 262 248 206 173 148
105 51 35 21 21
BanII G_rGCy'C
Cuts at: 205 223 281 512 808 1318 1654 2684 205
Size: 18 58 231 296 510 336 1030 2161
Fragments arranged by size:
2161 1030 510 336 296 231 58 18
BbeI G_GCGC'C
Cuts at: 170 879 1132 1183 1204 2074 2322 170
Size: 709 253 51 21 870 248 2488
Fragments arranged by size:
2488 870 709 253 248 51 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 456 490 456
Size: 34 4606
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3594 4640 2538
Size: 1056 1046 2538
Fragments arranged by size:
2538 1056 1046
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglI GCCn_nnn'nGGC
Cuts at: 164 218 1090 1168 1177 164
Size: 54 872 78 9 3627
Fragments arranged by size:
3627 872 78 54 9
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 511 940 1261 1312 1523 1752 511
Size: 429 321 51 211 229 3399
Fragments arranged by size:
3399 429 321 229 211 51
Bme1580I G_kGCm'C
Cuts at: 377 773 808 1486 2265 2358 3482 377
Size: 396 35 678 779 93 1124 1535
Fragments arranged by size:
1535 1124 779 678 396 93 35
BmgBI CAC'GTC
Cuts at: 420 840 924 420
Size: 420 84 4136
Fragments arranged by size:
4136 420 84
BmrI ACTGGGnnnn_n'
Cuts at: 1608 1852 1933 2268 4412 1608
Size: 244 81 335 2144 1836
Fragments arranged by size:
2144 1836 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 789 3007 789
Size: 2218 2422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 555 1434 384
Size: 171 879 3590
Fragments arranged by size:
3590 879 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2695 3409 3707 1816
Size: 879 714 298 2749
Fragments arranged by size:
2749 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 961 4344 961
Size: 3383 1257
BsaAI yAC'GTr
Cuts at: 1107 1993 1107
Size: 886 3754
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 167 876 950 1129 1180 1201 2071 2319 3021
Size: 709 74 179 51 21 870 248 702
Cuts at: 3021 4517 167
Size: 1496 290
Fragments arranged by size:
1496 870 709 702 290 248 179 74
51 21
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3439 3586 3860 1829
Size: 521 678 411 147 274 2609
Fragments arranged by size:
2609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 247 277 295 325 736 766 940 970 1381
Size: 30 18 30 411 30 174 30 411
Cuts at: 1381 1411 1432 1438 1462 1468 247
Size: 30 21 6 24 6 3419
Fragments arranged by size:
3419 411 411 174 30 30 30 30
30 24 21 18 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 316 589 691 738 741 316
Size: 273 102 47 3 4215
Fragments arranged by size:
4215 273 102 47 3
BseYI C'CCAG_C
Cuts at: 172 522 1283 1318 1592 2065 2823 3488 3896
Size: 350 761 35 274 473 758 665 408
Cuts at: 3896 172
Size: 916
Fragments arranged by size:
916 761 758 665 473 408 350 274
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 862 616
Size: 246 4394
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3458 104
Size: 2078 46 1230 1286
Fragments arranged by size:
2078 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 350 512 773 788 1654 2432 2622 3482 350
Size: 162 261 15 866 778 190 860 1508
Fragments arranged by size:
1508 866 860 778 261 190 162 15
Bsp1286I G_dGCh'C
Cuts at: 205 223 281 350 377 512 773 788 808
Size: 18 58 69 27 135 261 15 20
Cuts at: 808 1318 1486 1654 2265 2358 2432 2622 2684
Size: 510 168 168 611 93 74 190 62
Cuts at: 2684 3482 205
Size: 798 1363
Fragments arranged by size:
1363 798 611 510 261 190 168 168
135 93 74 69 62 58 27 20
18 15
BspHI T'CATG_A
Cuts at: 128 3072 128
Size: 2944 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1007 1690 1904 2206 2587 1007
Size: 683 214 302 381 3060
Fragments arranged by size:
3060 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 585 1418 1522 2932 2986 4470 4635 50
Size: 535 833 104 1410 54 1484 165 55
Fragments arranged by size:
1484 1410 833 535 165 104 55 54
BsrDI GCAATG_nn'
Cuts at: 2552 2552
Size: 4640
BsrFI r'CCGG_y
Cuts at: 381 1026 1167 1215 1486 2638 2819 381
Size: 645 141 48 271 1152 181 2202
Fragments arranged by size:
2202 1152 645 271 181 141 48
BsrGI T'GTAC_A
Cuts at: 1535 1535
Size: 4640
BssHII G'CGCG_C
Cuts at: 463 463
Size: 4640
BssSI C'ACGA_G
Cuts at: 2911 3619 2911
Size: 708 3932
BstAPI GCAn_nnn'nTGC
Cuts at: 2017 2017
Size: 4640
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstEII G'GTnAC_C
Cuts at: 1044 1044
Size: 4640
BstYI r'GATC_y
Cuts at: 1 1665 2132 2155 2490 2736 3042 3054 3140
Size: 1664 467 23 335 246 306 12 86
Cuts at: 3140 4192 1
Size: 1052 449
Fragments arranged by size:
1664 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 197 634 683 1079 197
Size: 437 49 396 3758
Fragments arranged by size:
3758 437 396 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1530 2590 2771 2934 1530
Size: 1060 181 163 3236
Fragments arranged by size:
3236 1060 181 163
BtsI GCAGTG_nn'
Cuts at: 1883 1883
Size: 4640
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3690 2346
Size: 1344 3296
EaeI y'GGCC_r
Cuts at: 1011 1028 1038 1082 2225 2399 2790 2817 4162
Size: 17 10 44 1143 174 391 27 1345
Cuts at: 4162 1011
Size: 1489
Fragments arranged by size:
1489 1345 1143 391 174 44 27 17
10
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 595 654 682 2848 3590 3736 4450 595
Size: 59 28 2166 742 146 714 785
Fragments arranged by size:
2166 785 742 714 146 59 28
EcoICRI GAG'CTC
Cuts at: 510 1652 510
Size: 1142 3498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 384 555 883 1434 2465 2897 3250 3912
Size: 77 171 328 551 1031 432 353 662
Cuts at: 3912 307
Size: 1035
Fragments arranged by size:
1035 1031 662 551 432 353 328 171
77
EcoO109I rG'GnC_Cy
Cuts at: 156 804 1161 1563 1779 156
Size: 648 357 402 216 3017
Fragments arranged by size:
3017 648 402 357 216
EcoRI G'AATT_C
Cuts at: 1644 1644
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 2659 582
Size: 2077 2563
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 170 727 879 1132 1183 1204 1530 2074 2322
Size: 557 152 253 51 21 326 544 248
Cuts at: 2322 3552 4323 170
Size: 1230 771 487
Fragments arranged by size:
1230 771 557 544 487 326 253 248
152 51 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 2482 2512 2514 2544 940
Size: 32 1510 30 2 30 3036
Fragments arranged by size:
3036 1510 32 30 30 2
HincII GTy'rAC
Cuts at: 393 981 1679 393
Size: 588 698 3354
Fragments arranged by size:
3354 698 588
KasI G'GCGC_C
Cuts at: 166 875 1128 1179 1200 2070 2318 166
Size: 709 253 51 21 870 248 2488
Fragments arranged by size:
2488 870 709 253 248 51 21
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 3399 3583 983
Size: 2416 184 2040
Fragments arranged by size:
2416 2040 184
MscI TGG'CCA
Cuts at: 2401 2401
Size: 4640
MslI CAynn'nnrTG
Cuts at: 133 382 1510 2629 2756 133
Size: 249 1128 1119 127 2017
Fragments arranged by size:
2017 1128 1119 249 127
MspA1I CmG'CkG
Cuts at: 199 636 1081 1311 1751 2065 2425 3209 3454
Size: 437 445 230 440 314 360 784 245
Cuts at: 3454 199
Size: 1385
Fragments arranged by size:
1385 784 445 440 437 360 314 245
230
NaeI GCC'GGC
Cuts at: 1028 1169 1217 2821 1028
Size: 141 48 1604 2847
Fragments arranged by size:
2847 1604 141 48
NarI GG'CG_CC
Cuts at: 167 876 1129 1180 1201 2071 2319 167
Size: 709 253 51 21 870 248 2488
Fragments arranged by size:
2488 870 709 253 248 51 21
NgoMIV G'CCGG_C
Cuts at: 1026 1167 1215 2819 1026
Size: 141 48 1604 2847
Fragments arranged by size:
2847 1604 141 48
NspI r_CATG'y
Cuts at: 1693 2193 3796 1693
Size: 500 1603 2537
Fragments arranged by size:
2537 1603 500
PasI CC'CwG_GG
Cuts at: 801 801
Size: 4640
PciI A'CATG_T
Cuts at: 3792 3792
Size: 4640
PfoI T'CCnGG_A
Cuts at: 116 116
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PmlI CAC'GTG
Cuts at: 1107 1107
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 279 295 327 1438 1470 3086 3118 247
Size: 32 16 32 1111 32 1616 32 1769
Fragments arranged by size:
1769 1616 1111 32 32 32 32 16
PpuMI rG'GwC_Cy
Cuts at: 1563 1563
Size: 4640
PspOMI G'GGCC_C
Cuts at: 804 804
Size: 4640
PstI C_TGCA'G
Cuts at: 731 1687 2372 731
Size: 956 685 2999
Fragments arranged by size:
2999 956 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 1311 2065 2425 1311
Size: 754 360 3526
Fragments arranged by size:
3526 754 360
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 512 1654 512
Size: 1142 3498
SacII CC_GC'GG
Cuts at: 200 637 1082 200
Size: 437 445 3758
Fragments arranged by size:
3758 445 437
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SanDI GG'GwC_CC
Cuts at: 1563 1563
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 2663 2873 2663
Size: 210 4430
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SfcI C'TryA_G
Cuts at: 33 727 1326 1365 1389 1461 1683 2368 3336
Size: 694 599 39 24 72 222 685 968
Cuts at: 3336 3527 33
Size: 191 1146
Fragments arranged by size:
1146 968 694 685 599 222 191 72
39 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 1168 1168
Size: 4640
SfoI GGC'GCC
Cuts at: 168 877 1130 1181 1202 2072 2320 168
Size: 709 253 51 21 870 248 2488
Fragments arranged by size:
2488 870 709 253 248 51 21
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1019 1662 1019
Size: 643 3997
SmlI C'TyrA_G
Cuts at: 1795 2710 3424 3686 1795
Size: 915 714 262 2749
Fragments arranged by size:
2749 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 269 1774 2128 269
Size: 1505 354 2781
Fragments arranged by size:
2781 1505 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 834 2223 2356 2815 3024 154
Size: 680 1389 133 459 209 1770
Fragments arranged by size:
1770 1389 680 459 209 133
TatI w'GTAC_w
Cuts at: 1535 4155 1535
Size: 2620 2020
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 736 768 1432 1464 4157 4189 736
Size: 32 664 32 2693 32 1187
Fragments arranged by size:
2693 1187 664 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2135 114
Size: 2021 2619
XmaI C'CCGG_G
Cuts at: 1017 1660 1017
Size: 643 3997
XmnI GAAnn'nnTTC
Cuts at: 262 439 262
Size: 177 4463
ZraI GAC'GTC
Cuts at: 951 951
Size: 4640
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI
BsmI BsmBI BspEI BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI