Restriction map for
pF1KB3929
(Circular) (Six-base) MAPSORT of: pF1KB3929.seq Check: 8752 from: 1 to: 3887
pF1KB3929 3887 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 925 925
Size: 3887
Acc65I G'GTAC_C
Cuts at: 903 903
Size: 3887
AclI AA'CG_TT
Cuts at: 3727 3727
Size: 3887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1712 2144 2891 3159 775
Size: 937 432 747 268 1503
Fragments arranged by size:
1503 937 747 432 268
AflIII A'CryG_T
Cuts at: 1239 2343 3320 1239
Size: 1104 977 1806
Fragments arranged by size:
1806 1104 977
AgeI A'CCGG_T
Cuts at: 2275 2275
Size: 3887
AlwNI CAG_nnn'CTG
Cuts at: 631 1192 2759 631
Size: 561 1567 1759
Fragments arranged by size:
1759 1567 561
ApaI G_GGCC'C
Cuts at: 212 212
Size: 3887
ApaLI G'TGCA_C
Cuts at: 2657 2657
Size: 3887
ApoI r'AATT_y
Cuts at: 891 3240 3496 891
Size: 2349 256 1282
Fragments arranged by size:
2349 1282 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
AvaI C'yCGr_G
Cuts at: 191 907 191
Size: 716 3171
BamHI G'GATC_C
Cuts at: 912 912
Size: 3887
BanI G'GyrC_C
Cuts at: 163 514 903 1317 1565 1600 163
Size: 351 389 414 248 35 2450
Fragments arranged by size:
2450 414 389 351 248 35
BanII G_rGCy'C
Cuts at: 212 350 901 1931 212
Size: 138 551 1030 2168
Fragments arranged by size:
2168 1030 551 138
BbeI G_GCGC'C
Cuts at: 167 1321 1569 167
Size: 1154 248 2485
Fragments arranged by size:
2485 1154 248
BbvCI CC'TCA_GC
Cuts at: 266 266
Size: 3887
BciVI GTATCCnnnnn_n'
Cuts at: 1785 2546 3887 1785
Size: 761 1341 1785
Fragments arranged by size:
1785 1341 761
BclI T'GATC_A
Cuts at: 1407 1407
Size: 3887
BglI GCCn_nnn'nGGC
Cuts at: 172 172
Size: 3887
BglII A'GATC_T
Cuts at: 1402 3439 1402
Size: 2037 1850
BlpI GC'TnA_GC
Cuts at: 999 999
Size: 3887
Bme1580I G_kGCm'C
Cuts at: 212 1512 1605 2661 212
Size: 1300 93 1056 1438
Fragments arranged by size:
1438 1300 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 754 1099 1180 1515 3659 754
Size: 345 81 335 2144 982
Fragments arranged by size:
2144 982 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 3887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 3887
Bpu10I CC'TnA_GC
Cuts at: 266 266
Size: 3887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1942 2434 2732 1063
Size: 879 492 298 2218
Fragments arranged by size:
2218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 188 487 3591 188
Size: 299 3104 484
Fragments arranged by size:
3104 484 299
BsaAI yAC'GTr
Cuts at: 1240 1240
Size: 3887
BsaBI GATnn'nnATC
Cuts at: 6 1401 3438 6
Size: 1395 2037 455
Fragments arranged by size:
2037 1395 455
BsaHI Gr'CG_yC
Cuts at: 164 188 1318 1566 2268 3764 164
Size: 24 1130 248 702 1496 287
Fragments arranged by size:
1496 1130 702 287 248 24
BsaWI w'CCGG_w
Cuts at: 1076 1597 2275 2549 2696 3107 1076
Size: 521 678 274 147 411 1856
Fragments arranged by size:
1856 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 377 805 358
Size: 19 428 3440
Fragments arranged by size:
3440 428 19
BseYI C'CCAG_C
Cuts at: 527 1312 2070 2647 3143 527
Size: 785 758 577 496 1271
Fragments arranged by size:
1271 785 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 172 278 1429 1475 2683 104
Size: 68 106 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 106 68 46
BsiHKAI G_wGCw'C
Cuts at: 350 901 1679 1869 2661 350
Size: 551 778 190 792 1576
Fragments arranged by size:
1576 792 778 551 190
Bsp1286I G_dGCh'C
Cuts at: 212 350 901 1512 1605 1679 1869 1931 2661
Size: 138 551 611 93 74 190 62 730
Cuts at: 2661 212
Size: 1438
Fragments arranged by size:
1438 730 611 551 190 138 93 74
62
BspEI T'CCGG_A
Cuts at: 3107 3107
Size: 3887
BspHI T'CATG_A
Cuts at: 3063 3063
Size: 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 937 1151 1453 1834 281
Size: 656 214 302 381 2334
Fragments arranged by size:
2334 656 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2179 2233 3717 3882 50
Size: 2129 54 1484 165 55
Fragments arranged by size:
2129 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 1799
Size: 3887
BsrFI r'CCGG_y
Cuts at: 171 812 1885 2066 2275 171
Size: 641 1073 181 209 1783
Fragments arranged by size:
1783 1073 641 209 181
BssSI C'ACGA_G
Cuts at: 2158 2516 2158
Size: 358 3529
BstAPI GCAn_nnn'nTGC
Cuts at: 240 1264 240
Size: 1024 2863
BstBI TT'CG_AA
Cuts at: 2248 2248
Size: 3887
BstXI CCAn_nnnn'nTGG
Cuts at: 744 744
Size: 3887
BstYI r'GATC_y
Cuts at: 1 912 1379 1402 1737 1983 2995 3081 3093
Size: 911 467 23 335 246 1012 86 12
Cuts at: 3093 3439 1
Size: 346 449
Fragments arranged by size:
1012 911 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 221 272 737 221
Size: 51 465 3371
Fragments arranged by size:
3371 465 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1837 2018 2181 1837
Size: 181 163 3543
Fragments arranged by size:
3543 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1130
Size: 3887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 809 844 809
Size: 35 3852
DraI TTT'AAA
Cuts at: 886 886
Size: 3887
DrdI GACnn_nn'nnGTC
Cuts at: 749 1593 2451 749
Size: 844 858 2185
Fragments arranged by size:
2185 858 844
EaeI y'GGCC_r
Cuts at: 169 275 1472 1646 2037 2064 3409 169
Size: 106 1197 174 391 27 1345 647
Fragments arranged by size:
1345 1197 647 391 174 106 27
EagI C'GGCC_G
Cuts at: 169 275 1472 169
Size: 106 1197 2584
Fragments arranged by size:
2584 1197 106
EarI CTCTTCn'nnn_
Cuts at: 342 1910 2120 342
Size: 1568 210 2109
Fragments arranged by size:
2109 1568 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2095 2405 2551 3697 2095
Size: 310 146 1146 2285
Fragments arranged by size:
2285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 348 899 348
Size: 551 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 775 1712 2144 2891 3159 391
Size: 384 937 432 747 268 1119
Fragments arranged by size:
1119 937 747 432 384 268
EcoO109I rG'GnC_Cy
Cuts at: 252 293 1026 252
Size: 41 733 3113
Fragments arranged by size:
3113 733 41
EcoRI G'AATT_C
Cuts at: 891 891
Size: 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1906 1906
Size: 3887
FseI GG_CCGG'CC
Cuts at: 175 175
Size: 3887
FspI TGC'GCA
Cuts at: 1668 1668
Size: 3887
HaeII r_GCGC'y
Cuts at: 167 1321 1569 2591 3570 167
Size: 1154 248 1022 979 484
Fragments arranged by size:
1154 1022 979 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 750 1729 1759 1761 1791 718
Size: 32 979 30 2 30 2814
Fragments arranged by size:
2814 979 32 30 30 2
HincII GTy'rAC
Cuts at: 846 926 846
Size: 80 3807
KasI G'GCGC_C
Cuts at: 163 1317 1565 163
Size: 1154 248 2485
Fragments arranged by size:
2485 1154 248
KpnI G_GTAC'C
Cuts at: 907 907
Size: 3887
MluI A'CGCG_T
Cuts at: 3320 3320
Size: 3887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2742 2558
Size: 184 3703
MscI TGG'CCA
Cuts at: 1648 1648
Size: 3887
MslI CAynn'nnrTG
Cuts at: 1876 2003 1876
Size: 127 3760
MspA1I CmG'CkG
Cuts at: 223 998 1312 1672 2685 2930 223
Size: 775 314 360 1013 245 1180
Fragments arranged by size:
1180 1013 775 360 314 245
NaeI GCC'GGC
Cuts at: 173 2068 173
Size: 1895 1992
NarI GG'CG_CC
Cuts at: 164 1318 1566 164
Size: 1154 248 2485
Fragments arranged by size:
2485 1154 248
NcoI C'CATG_G
Cuts at: 737 737
Size: 3887
NgoMIV G'CCGG_C
Cuts at: 171 2066 171
Size: 1895 1992
NspI r_CATG'y
Cuts at: 940 1440 2347 940
Size: 500 907 2480
Fragments arranged by size:
2480 907 500
PasI CC'CwG_GG
Cuts at: 768 768
Size: 3887
PciI A'CATG_T
Cuts at: 2343 2343
Size: 3887
PfoI T'CCnGG_A
Cuts at: 368 368
Size: 3887
PmeI GTTT'AAAC
Cuts at: 886 886
Size: 3887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 3026
Size: 32 3855
PpuMI rG'GwC_Cy
Cuts at: 252 293 252
Size: 41 3846
PspOMI G'GGCC_C
Cuts at: 208 208
Size: 3887
PstI C_TGCA'G
Cuts at: 934 1619 934
Size: 685 3202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3887
PvuII CAG'CTG
Cuts at: 1312 1672 1312
Size: 360 3527
RsrII CG'GwC_CG
Cuts at: 2082 2082
Size: 3887
SacI G_AGCT'C
Cuts at: 350 901 350
Size: 551 3336
SacII CC_GC'GG
Cuts at: 224 224
Size: 3887
SalI G'TCGA_C
Cuts at: 924 924
Size: 3887
SapI GCTCTTCn'nnn_
Cuts at: 1910 2120 1910
Size: 210 3677
SbfI CC_TGCA'GG
Cuts at: 934 934
Size: 3887
ScaI AGT'ACT
Cuts at: 238 663 3404 238
Size: 425 2741 721
Fragments arranged by size:
2741 721 425
SfcI C'TryA_G
Cuts at: 33 930 1615 2608 2799 33
Size: 897 685 993 191 1121
Fragments arranged by size:
1121 993 897 685 191
SfoI GGC'GCC
Cuts at: 165 1319 1567 165
Size: 1154 248 2485
Fragments arranged by size:
2485 1154 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3887
SmaI CCC'GGG
Cuts at: 909 909
Size: 3887
SmlI C'TyrA_G
Cuts at: 1042 1957 2449 2711 1042
Size: 915 492 262 2218
Fragments arranged by size:
2218 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3887
SphI G_CATG'C
Cuts at: 940 1440 940
Size: 500 3387
StyI C'CwwG_G
Cuts at: 178 737 1021 1375 178
Size: 559 284 354 2690
Fragments arranged by size:
2690 559 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 614 1470 1603 2062 2271 614
Size: 856 133 459 209 2230
Fragments arranged by size:
2230 856 459 209 133
TatI w'GTAC_w
Cuts at: 236 559 661 3402 236
Size: 323 102 2741 721
Fragments arranged by size:
2741 721 323 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 3855
Tth111I GACn'n_nGTC
Cuts at: 631 746 1684 631
Size: 115 938 2834
Fragments arranged by size:
2834 938 115
XbaI T'CTAG_A
Cuts at: 918 918
Size: 3887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 1382 294
Size: 1088 2799
XmaI C'CCGG_G
Cuts at: 907 907
Size: 3887
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI