Restriction map for
pF1KB3876
(Circular) (Six-base) MAPSORT of: pF1KB3876.seq Check: 568 from: 1 to: 3608
pF1KB3876 3608 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 646 646
Size: 3608
Acc65I G'GTAC_C
Cuts at: 314 624 314
Size: 310 3298
AclI AA'CG_TT
Cuts at: 3448 3448
Size: 3608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 1865 2612 2880 1433
Size: 432 747 268 2161
Fragments arranged by size:
2161 747 432 268
AflIII A'CryG_T
Cuts at: 960 2064 3041 960
Size: 1104 977 1527
Fragments arranged by size:
1527 1104 977
AgeI A'CCGG_T
Cuts at: 1996 1996
Size: 3608
AlwNI CAG_nnn'CTG
Cuts at: 409 913 2480 409
Size: 504 1567 1537
Fragments arranged by size:
1567 1537 504
ApaLI G'TGCA_C
Cuts at: 2378 2378
Size: 3608
ApoI r'AATT_y
Cuts at: 553 612 2961 3217 553
Size: 59 2349 256 944
Fragments arranged by size:
2349 944 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 3608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
AvaI C'yCGr_G
Cuts at: 269 628 269
Size: 359 3249
BamHI G'GATC_C
Cuts at: 633 633
Size: 3608
BanI G'GyrC_C
Cuts at: 314 511 624 1038 1286 1321 314
Size: 197 113 414 248 35 2601
Fragments arranged by size:
2601 414 248 197 113 35
BanII G_rGCy'C
Cuts at: 622 1652 622
Size: 1030 2578
BbeI G_GCGC'C
Cuts at: 1042 1290 1042
Size: 248 3360
BciVI GTATCCnnnnn_n'
Cuts at: 343 1506 2267 3608 343
Size: 1163 761 1341 343
Fragments arranged by size:
1341 1163 761 343
BclI T'GATC_A
Cuts at: 1128 1128
Size: 3608
BglII A'GATC_T
Cuts at: 1123 3160 1123
Size: 2037 1571
BlpI GC'TnA_GC
Cuts at: 720 720
Size: 3608
Bme1580I G_kGCm'C
Cuts at: 230 1233 1326 2382 230
Size: 1003 93 1056 1456
Fragments arranged by size:
1456 1056 1003 93
BmgBI CAC'GTC
Cuts at: 586 586
Size: 3608
BmrI ACTGGGnnnn_n'
Cuts at: 820 901 1236 3380 820
Size: 81 335 2144 1048
Fragments arranged by size:
2144 1048 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 454 318
Size: 136 3472
Bpu10I CC'TnA_GC
Cuts at: 197 197
Size: 3608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 784 1663 2155 2453 247
Size: 537 879 492 298 1402
Fragments arranged by size:
1402 879 537 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3312 3312
Size: 3608
BsaAI yAC'GTr
Cuts at: 279 961 279
Size: 682 2926
BsaBI GATnn'nnATC
Cuts at: 6 1122 3159 6
Size: 1116 2037 455
Fragments arranged by size:
2037 1116 455
BsaHI Gr'CG_yC
Cuts at: 1039 1287 1989 3485 1039
Size: 248 702 1496 1162
Fragments arranged by size:
1496 1162 702 248
BsaWI w'CCGG_w
Cuts at: 128 797 1318 1996 2270 2417 2828 128
Size: 669 521 678 274 147 411 908
Fragments arranged by size:
908 678 669 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 155 185 155
Size: 30 3578
BseYI C'CCAG_C
Cuts at: 532 538 1033 1791 2368 2864 532
Size: 6 495 758 577 496 1276
Fragments arranged by size:
1276 758 577 496 495 6
BsiEI CG_ry'CG
Cuts at: 104 1150 1196 2404 104
Size: 1046 46 1208 1308
Fragments arranged by size:
1308 1208 1046 46
BsiHKAI G_wGCw'C
Cuts at: 622 1400 1590 2382 622
Size: 778 190 792 1848
Fragments arranged by size:
1848 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 230 622 1233 1326 1400 1590 1652 2382 230
Size: 392 611 93 74 190 62 730 1456
Fragments arranged by size:
1456 730 611 392 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2828 2828
Size: 3608
BspHI T'CATG_A
Cuts at: 242 2784 242
Size: 2542 1066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 456 658 872 1174 1555 456
Size: 202 214 302 381 2509
Fragments arranged by size:
2509 381 302 214 202
BsrBI CCG'CTC
Cuts at: 50 1900 1954 3438 3603 50
Size: 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1520 1520
Size: 3608
BsrFI r'CCGG_y
Cuts at: 1606 1787 1996 1606
Size: 181 209 3218
Fragments arranged by size:
3218 209 181
BssSI C'ACGA_G
Cuts at: 1879 2237 1879
Size: 358 3250
BstAPI GCAn_nnn'nTGC
Cuts at: 985 985
Size: 3608
BstBI TT'CG_AA
Cuts at: 1969 1969
Size: 3608
BstYI r'GATC_y
Cuts at: 1 633 1100 1123 1458 1704 2716 2802 2814
Size: 632 467 23 335 246 1012 86 12
Cuts at: 2814 3160 1
Size: 346 449
Fragments arranged by size:
1012 632 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 599 599
Size: 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1558 1739 1902 1558
Size: 181 163 3264
Fragments arranged by size:
3264 181 163
BtsI GCAGTG_nn'
Cuts at: 851 851
Size: 3608
DraI TTT'AAA
Cuts at: 607 607
Size: 3608
DraIII CAC_nnn'GTG
Cuts at: 511 511
Size: 3608
DrdI GACnn_nn'nnGTC
Cuts at: 1314 2172 1314
Size: 858 2750
EaeI y'GGCC_r
Cuts at: 173 1193 1367 1758 1785 3130 173
Size: 1020 174 391 27 1345 651
Fragments arranged by size:
1345 1020 651 391 174 27
EagI C'GGCC_G
Cuts at: 1193 1193
Size: 3608
EarI CTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 584 1816 2126 2272 3418 584
Size: 1232 310 146 1146 774
Fragments arranged by size:
1232 1146 774 310 146
EcoICRI GAG'CTC
Cuts at: 620 620
Size: 3608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 454 1433 1865 2612 2880 318
Size: 136 979 432 747 268 1046
Fragments arranged by size:
1046 979 747 432 268 136
EcoNI CCTnn'n_nnAGG
Cuts at: 154 154
Size: 3608
EcoO109I rG'GnC_Cy
Cuts at: 747 747
Size: 3608
EcoRI G'AATT_C
Cuts at: 553 612 553
Size: 59 3549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1627 1627
Size: 3608
FspI TGC'GCA
Cuts at: 1389 1389
Size: 3608
HaeII r_GCGC'y
Cuts at: 1042 1290 2312 3291 1042
Size: 248 1022 979 1359
Fragments arranged by size:
1359 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1480 1482 1512 1450
Size: 30 2 30 3546
Fragments arranged by size:
3546 30 30 2
HincII GTy'rAC
Cuts at: 647 647
Size: 3608
KasI G'GCGC_C
Cuts at: 1038 1286 1038
Size: 248 3360
KpnI G_GTAC'C
Cuts at: 318 628 318
Size: 310 3298
MluI A'CGCG_T
Cuts at: 3041 3041
Size: 3608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2463 2279
Size: 184 3424
MscI TGG'CCA
Cuts at: 175 1369 175
Size: 1194 2414
MslI CAynn'nnrTG
Cuts at: 112 1597 1724 112
Size: 1485 127 1996
Fragments arranged by size:
1996 1485 127
MspA1I CmG'CkG
Cuts at: 719 1033 1393 2406 2651 719
Size: 314 360 1013 245 1676
Fragments arranged by size:
1676 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1789 1789
Size: 3608
NarI GG'CG_CC
Cuts at: 1039 1287 1039
Size: 248 3360
NgoMIV G'CCGG_C
Cuts at: 1787 1787
Size: 3608
NspI r_CATG'y
Cuts at: 195 661 1161 2068 195
Size: 466 500 907 1735
Fragments arranged by size:
1735 907 500 466
PciI A'CATG_T
Cuts at: 2064 2064
Size: 3608
PflMI CCAn_nnn'nTGG
Cuts at: 409 409
Size: 3608
PmeI GTTT'AAAC
Cuts at: 607 607
Size: 3608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 3576
PstI C_TGCA'G
Cuts at: 655 1340 655
Size: 685 2923
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3608
PvuII CAG'CTG
Cuts at: 1033 1393 1033
Size: 360 3248
RsrII CG'GwC_CG
Cuts at: 1803 1803
Size: 3608
SacI G_AGCT'C
Cuts at: 622 622
Size: 3608
SalI G'TCGA_C
Cuts at: 645 645
Size: 3608
SapI GCTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
SbfI CC_TGCA'GG
Cuts at: 655 655
Size: 3608
ScaI AGT'ACT
Cuts at: 3125 3125
Size: 3608
SexAI A'CCwGG_T
Cuts at: 507 507
Size: 3608
SfcI C'TryA_G
Cuts at: 33 486 651 1336 2329 2520 33
Size: 453 165 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 191 165
SfoI GGC'GCC
Cuts at: 1040 1288 1040
Size: 248 3360
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
SmaI CCC'GGG
Cuts at: 630 630
Size: 3608
SmlI C'TyrA_G
Cuts at: 262 763 1678 2170 2432 262
Size: 501 915 492 262 1438
Fragments arranged by size:
1438 915 501 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3608
SphI G_CATG'C
Cuts at: 661 1161 661
Size: 500 3108
StuI AGG'CCT
Cuts at: 417 417
Size: 3608
StyI C'CwwG_G
Cuts at: 742 1096 742
Size: 354 3254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1191 1324 1783 1992 1191
Size: 133 459 209 2807
Fragments arranged by size:
2807 459 209 133
TatI w'GTAC_w
Cuts at: 3123 3123
Size: 3608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 3125 3157 155
Size: 32 2938 32 606
Fragments arranged by size:
2938 606 32 32
Tth111I GACn'n_nGTC
Cuts at: 587 1405 587
Size: 818 2790
XbaI T'CTAG_A
Cuts at: 639 639
Size: 3608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1103 1103
Size: 3608
XmaI C'CCGG_G
Cuts at: 628 628
Size: 3608
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI