Restriction map for
pF1KB3864
(Circular) (Six-base) MAPSORT of: pF1KB3864.seq Check: 653 from: 1 to: 6539
pF1KB3864 6539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 927 3322 927
Size: 2395 4144
AccI GT'mk_AC
Cuts at: 3577 3577
Size: 6539
Acc65I G'GTAC_C
Cuts at: 1913 3555 1913
Size: 1642 4897
AclI AA'CG_TT
Cuts at: 6379 6379
Size: 6539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1366 1388 1868 4364 4796 5543 5811 1126
Size: 240 22 480 2496 432 747 268 1854
Fragments arranged by size:
2496 1854 747 480 432 268 240 22
AfeI AGC'GCT
Cuts at: 2740 2740
Size: 6539
AflIII A'CryG_T
Cuts at: 3891 4995 5972 3891
Size: 1104 977 4458
Fragments arranged by size:
4458 1104 977
AgeI A'CCGG_T
Cuts at: 4927 4927
Size: 6539
AleI CACnn'nnGTG
Cuts at: 1387 2080 3091 1387
Size: 693 1011 4835
Fragments arranged by size:
4835 1011 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2482 3355 2482
Size: 873 5666
AlwNI CAG_nnn'CTG
Cuts at: 562 969 2121 2196 2697 2806 3228 3459 3844
Size: 407 1152 75 501 109 422 231 385
Cuts at: 3844 5411 562
Size: 1567 1690
Fragments arranged by size:
1690 1567 1152 501 422 407 385 231
109 75
ApaI G_GGCC'C
Cuts at: 685 819 1520 2421 2485 685
Size: 134 701 901 64 4739
Fragments arranged by size:
4739 901 701 134 64
ApaLI G'TGCA_C
Cuts at: 5309 5309
Size: 6539
ApoI r'AATT_y
Cuts at: 3543 5892 6148 3543
Size: 2349 256 3934
Fragments arranged by size:
3934 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
AvaI C'yCGr_G
Cuts at: 133 283 812 818 2493 2539 3559 133
Size: 150 529 6 1675 46 1020 3113
Fragments arranged by size:
3113 1675 1020 529 150 46 6
BamHI G'GATC_C
Cuts at: 3149 3564 3149
Size: 415 6124
BanI G'GyrC_C
Cuts at: 139 165 939 1230 1907 1913 3402 3555 3969
Size: 26 774 291 677 6 1489 153 414
Cuts at: 3969 4217 4252 139
Size: 248 35 2426
Fragments arranged by size:
2426 1489 774 677 414 291 248 153
35 26 6
BanII G_rGCy'C
Cuts at: 182 572 685 703 819 862 1168 1520 1834
Size: 390 113 18 116 43 306 352 314
Cuts at: 1834 1876 2115 2190 2421 2485 2717 2902 2948
Size: 42 239 75 231 64 232 185 46
Cuts at: 2948 3553 4583 182
Size: 605 1030 2138
Fragments arranged by size:
2138 1030 605 390 352 314 306 239
232 231 185 116 113 75 64 46
43 42 18
BbeI G_GCGC'C
Cuts at: 143 1911 3973 4221 143
Size: 1768 2062 248 2461
Fragments arranged by size:
2461 2062 1768 248
BbsI GAAGACnn'nnnn_
Cuts at: 2041 2235 3295 2041
Size: 194 1060 5285
Fragments arranged by size:
5285 1060 194
BbvCI CC'TCA_GC
Cuts at: 733 1376 1996 733
Size: 643 620 5276
Fragments arranged by size:
5276 643 620
BciVI GTATCCnnnnn_n'
Cuts at: 3220 4437 5198 6539 3220
Size: 1217 761 1341 3220
Fragments arranged by size:
3220 1341 1217 761
BclI T'GATC_A
Cuts at: 4059 4059
Size: 6539
BfrBI ATG'CAT
Cuts at: 3303 3303
Size: 6539
BglI GCCn_nnn'nGGC
Cuts at: 1606 2260 2712 3113 3411 1606
Size: 654 452 401 298 4734
Fragments arranged by size:
4734 654 452 401 298
BglII A'GATC_T
Cuts at: 2312 4054 6091 2312
Size: 1742 2037 2760
Fragments arranged by size:
2760 2037 1742
BlpI GC'TnA_GC
Cuts at: 2114 3651 2114
Size: 1537 5002
Bme1580I G_kGCm'C
Cuts at: 170 685 819 898 909 944 1520 2421 2466
Size: 515 134 79 11 35 576 901 45
Cuts at: 2466 2485 2772 4164 4257 5313 170
Size: 19 287 1392 93 1056 1396
Fragments arranged by size:
1396 1392 1056 901 576 515 287 134
93 79 45 35 19 11
BmgBI CAC'GTC
Cuts at: 198 3197 3293 198
Size: 2999 96 3444
Fragments arranged by size:
3444 2999 96
BmrI ACTGGGnnnn_n'
Cuts at: 1922 1950 3751 3832 4167 6311 1922
Size: 28 1801 81 335 2144 2150
Fragments arranged by size:
2150 2144 1801 335 81 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 4906 468
Size: 4438 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 696 876 1030 1152 1375 1483 2218 2443
Size: 172 180 154 122 223 108 735 225
Cuts at: 2443 2467 524
Size: 24 4596
Fragments arranged by size:
4596 735 225 223 180 172 154 122
108 24
Bpu10I CC'TnA_GC
Cuts at: 733 1376 1735 1996 3263 733
Size: 643 359 261 1267 4009
Fragments arranged by size:
4009 1267 643 359 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2878 3715 4594 5086 5384 1495
Size: 1383 837 879 492 298 2650
Fragments arranged by size:
2650 1383 879 837 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2233 2344 6243 2233
Size: 111 3899 2529
Fragments arranged by size:
3899 2529 111
BsaAI yAC'GTr
Cuts at: 1093 3892 1093
Size: 2799 3740
BsaBI GATnn'nnATC
Cuts at: 6 4053 6090 6
Size: 4047 2037 455
Fragments arranged by size:
4047 2037 455
BsaHI Gr'CG_yC
Cuts at: 140 1289 1908 2090 3970 4218 4920 6416 140
Size: 1149 619 182 1880 248 702 1496 263
Fragments arranged by size:
1880 1496 1149 702 619 263 248 182
BsaWI w'CCGG_w
Cuts at: 2902 3728 4249 4927 5201 5348 5759 2902
Size: 826 521 678 274 147 411 3682
Fragments arranged by size:
3682 826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 441 471 491 521 877 907 1062 1092 441
Size: 30 20 30 356 30 155 30 5888
Fragments arranged by size:
5888 356 155 30 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 217 220 449 505 535 544 2809 2914
Size: 27 3 229 56 30 9 2265 105
Cuts at: 2914 190
Size: 3815
Fragments arranged by size:
3815 2265 229 105 56 30 27 9
3
BseYI C'CCAG_C
Cuts at: 292 704 796 805 835 969 1081 1207 1512
Size: 412 92 9 30 134 112 126 305
Cuts at: 1512 1519 2025 2106 2488 3221 3324 3964 4722
Size: 7 506 81 382 733 103 640 758
Cuts at: 4722 5299 5795 292
Size: 577 496 1036
Fragments arranged by size:
1036 758 733 640 577 506 496 412
382 305 134 126 112 103 92 81
30 9 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 3505 1933
Size: 1572 4967
BsiEI CG_ry'CG
Cuts at: 104 4081 4127 5335 104
Size: 3977 46 1208 1308
Fragments arranged by size:
3977 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 727 1168 1976 2087 2115 2387 3098 3167 3553
Size: 441 808 111 28 272 711 69 386
Cuts at: 3553 4331 4521 5313 727
Size: 778 190 792 1953
Fragments arranged by size:
1953 808 792 778 711 441 386 272
190 111 69 28
Bsp1286I G_dGCh'C
Cuts at: 170 182 572 685 703 727 819 862 898
Size: 12 390 113 18 24 92 43 36
Cuts at: 898 909 944 1168 1520 1834 1876 1976 2087
Size: 11 35 224 352 314 42 100 111
Cuts at: 2087 2115 2190 2387 2421 2466 2485 2717 2772
Size: 28 75 197 34 45 19 232 55
Cuts at: 2772 2902 2948 3098 3167 3553 4164 4257 4331
Size: 130 46 150 69 386 611 93 74
Cuts at: 4331 4521 4583 5313 170
Size: 190 62 730 1396
Fragments arranged by size:
1396 730 611 390 386 352 314 232
224 197 190 150 130 113 111 100
93 92 75 74 69 62 55 46
45 43 42 36 35 34 28 24
19 18 12 11
BspEI T'CCGG_A
Cuts at: 5759 5759
Size: 6539
BspHI T'CATG_A
Cuts at: 1590 5715 1590
Size: 4125 2414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 887 927 1706 1943 2927 3322 3589 3803
Size: 654 40 779 237 984 395 267 214
Cuts at: 3803 4105 4486 233
Size: 302 381 2286
Fragments arranged by size:
2286 984 779 654 395 381 302 267
237 214 40
BsrBI CCG'CTC
Cuts at: 50 312 360 2993 4831 4885 6369 6534 50
Size: 262 48 2633 1838 54 1484 165 55
Fragments arranged by size:
2633 1838 1484 262 165 55 54 48
BsrDI GCAATG_nn'
Cuts at: 1559 3414 4451 1559
Size: 1855 1037 3647
Fragments arranged by size:
3647 1855 1037
BsrFI r'CCGG_y
Cuts at: 329 848 1616 1910 3246 4537 4718 4927 329
Size: 519 768 294 1336 1291 181 209 1941
Fragments arranged by size:
1941 1336 1291 768 519 294 209 181
BsrGI T'GTAC_A
Cuts at: 3182 3182
Size: 6539
BssHII G'CGCG_C
Cuts at: 367 367
Size: 6539
BssSI C'ACGA_G
Cuts at: 1543 2742 3109 4810 5168 1543
Size: 1199 367 1701 358 2914
Fragments arranged by size:
2914 1701 1199 367 358
BstAPI GCAn_nnn'nTGC
Cuts at: 722 939 2391 3916 722
Size: 217 1452 1525 3345
Fragments arranged by size:
3345 1525 1452 217
BstBI TT'CG_AA
Cuts at: 4900 4900
Size: 6539
BstEII G'GTnAC_C
Cuts at: 1327 1327
Size: 6539
BstXI CCAn_nnnn'nTGG
Cuts at: 759 1315 3111 3129 759
Size: 556 1796 18 4169
Fragments arranged by size:
4169 1796 556 18
BstYI r'GATC_y
Cuts at: 1 1361 2312 3059 3149 3173 3564 4031 4054
Size: 1360 951 747 90 24 391 467 23
Cuts at: 4054 4389 4635 5647 5733 5745 6091 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1360 1012 951 747 467 449 391 346
335 246 90 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 579 2411 579
Size: 1832 4707
BtgI C'CryG_G
Cuts at: 107 1212 1238 1382 1709 2785 2984 3359 107
Size: 1105 26 144 327 1076 199 375 3287
Fragments arranged by size:
3287 1105 1076 375 327 199 144 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3021 3084 3318 4489 4670 4833 3021
Size: 63 234 1171 181 163 4727
Fragments arranged by size:
4727 1171 234 181 163 63
BtsI GCAGTG_nn'
Cuts at: 909 2389 2466 2515 2772 3337 3782 909
Size: 1480 77 49 257 565 445 3666
Fragments arranged by size:
3666 1480 565 445 257 77 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 903 938 1119 1154 903
Size: 35 181 35 6288
Fragments arranged by size:
6288 181 35 35
DraI TTT'AAA
Cuts at: 3538 3538
Size: 6539
DraIII CAC_nnn'GTG
Cuts at: 1041 1786 1041
Size: 745 5794
DrdI GACnn_nn'nnGTC
Cuts at: 4245 5103 4245
Size: 858 5681
EaeI y'GGCC_r
Cuts at: 110 866 1273 1964 2211 2261 3498 4124 4298
Size: 756 407 691 247 50 1237 626 174
Cuts at: 4298 4689 4716 6061 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 1237 756 691 626 588 407 391
247 174 50 27
EagI C'GGCC_G
Cuts at: 4124 4124
Size: 6539
EarI CTCTTCn'nnn_
Cuts at: 512 521 530 991 1507 1981 2101 3439 4562
Size: 9 9 461 516 474 120 1338 1123
Cuts at: 4562 4772 512
Size: 210 2279
Fragments arranged by size:
2279 1338 1123 516 474 461 210 120
9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 970 3312 4747 5057 5203 6349 970
Size: 2342 1435 310 146 1146 1160
Fragments arranged by size:
2342 1435 1160 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1166 2113 3551 1166
Size: 947 1438 4154
Fragments arranged by size:
4154 1438 947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 696 876 1030 1126 1152 1366 1375 1388
Size: 172 180 154 96 26 214 9 13
Cuts at: 1388 1483 1868 2218 2443 2467 4364 4796 5543
Size: 95 385 350 225 24 1897 432 747
Cuts at: 5543 5811 524
Size: 268 1252
Fragments arranged by size:
1897 1252 747 432 385 350 268 225
214 180 172 154 96 95 26 24
13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 154 154
Size: 6539
EcoO109I rG'GnC_Cy
Cuts at: 252 279 473 584 1479 1516 2193 2417 2418
Size: 27 194 111 895 37 677 224 1
Cuts at: 2418 2429 2481 2482 2543 3678 252
Size: 11 52 1 61 1135 3113
Fragments arranged by size:
3113 1135 895 677 224 194 111 61
52 37 27 11 1 1
EcoRI G'AATT_C
Cuts at: 3543 3543
Size: 6539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4558 4558
Size: 6539
FspI TGC'GCA
Cuts at: 2569 4320 2569
Size: 1751 4788
HaeII r_GCGC'y
Cuts at: 143 1557 1911 2742 2863 3460 3973 4221 5243
Size: 1414 354 831 121 597 513 248 1022
Cuts at: 5243 6222 143
Size: 979 460
Fragments arranged by size:
1414 1022 979 831 597 513 460 354
248 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1062 1094 2725 2757 4381 4411 4413 4443 1062
Size: 32 1631 32 1624 30 2 30 3158
Fragments arranged by size:
3158 1631 1624 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3017 3578 3017
Size: 561 5978
KasI G'GCGC_C
Cuts at: 139 1907 3969 4217 139
Size: 1768 2062 248 2461
Fragments arranged by size:
2461 2062 1768 248
KpnI G_GTAC'C
Cuts at: 1917 3559 1917
Size: 1642 4897
MluI A'CGCG_T
Cuts at: 5972 5972
Size: 6539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3127 5210 5394 3127
Size: 2083 184 4272
Fragments arranged by size:
4272 2083 184
MscI TGG'CCA
Cuts at: 112 1275 3500 4300 112
Size: 1163 2225 800 2351
Fragments arranged by size:
2351 2225 1163 800
MslI CAynn'nnrTG
Cuts at: 1387 1570 2080 2533 2647 3008 3091 3127 3241
Size: 183 510 453 114 361 83 36 114
Cuts at: 3241 3529 4528 4655 1387
Size: 288 999 127 3271
Fragments arranged by size:
3271 999 510 453 361 288 183 127
114 114 83 36
MspA1I CmG'CkG
Cuts at: 1969 2080 2121 2148 2806 3081 3216 3324 3650
Size: 111 41 27 658 275 135 108 326
Cuts at: 3650 3964 4324 5337 5582 1969
Size: 314 360 1013 245 2926
Fragments arranged by size:
2926 1013 658 360 326 314 275 245
135 111 108 41 27
NaeI GCC'GGC
Cuts at: 850 3248 4720 850
Size: 2398 1472 2669
Fragments arranged by size:
2669 2398 1472
NarI GG'CG_CC
Cuts at: 140 1908 3970 4218 140
Size: 1768 2062 248 2461
Fragments arranged by size:
2461 2062 1768 248
NcoI C'CATG_G
Cuts at: 107 1212 1238 107
Size: 1105 26 5408
Fragments arranged by size:
5408 1105 26
NgoMIV G'CCGG_C
Cuts at: 848 3246 4718 848
Size: 2398 1472 2669
Fragments arranged by size:
2669 2398 1472
NsiI A_TGCA'T
Cuts at: 3305 3305
Size: 6539
NspI r_CATG'y
Cuts at: 1314 3528 3592 4092 4999 1314
Size: 2214 64 500 907 2854
Fragments arranged by size:
2854 2214 907 500 64
PasI CC'CwG_GG
Cuts at: 643 909 1801 2760 2937 643
Size: 266 892 959 177 4245
Fragments arranged by size:
4245 959 892 266 177
PciI A'CATG_T
Cuts at: 4995 4995
Size: 6539
PflMI CCAn_nnn'nTGG
Cuts at: 969 1962 2196 969
Size: 993 234 5312
Fragments arranged by size:
5312 993 234
PfoI T'CCnGG_A
Cuts at: 485 1054 1460 1649 3062 3437 485
Size: 569 406 189 1413 375 3587
Fragments arranged by size:
3587 1413 569 406 375 189
PmeI GTTT'AAAC
Cuts at: 3538 3538
Size: 6539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5678 5710 5678
Size: 32 6507
PpuMI rG'GwC_Cy
Cuts at: 252 279 473 584 1479 2193 2429 2543 252
Size: 27 194 111 895 714 236 114 4248
Fragments arranged by size:
4248 895 714 236 194 114 111 27
PshAI GACnn'nnGTC
Cuts at: 1765 1765
Size: 6539
PspOMI G'GGCC_C
Cuts at: 681 815 1516 2417 2481 681
Size: 134 701 901 64 4739
Fragments arranged by size:
4739 901 701 134 64
PstI C_TGCA'G
Cuts at: 981 1428 3455 3586 4271 981
Size: 447 2027 131 685 3249
Fragments arranged by size:
3249 2027 685 447 131
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6539
PvuII CAG'CTG
Cuts at: 3216 3324 3964 4324 3216
Size: 108 640 360 5431
Fragments arranged by size:
5431 640 360 108
RsrII CG'GwC_CG
Cuts at: 1491 2988 4734 1491
Size: 1497 1746 3296
Fragments arranged by size:
3296 1746 1497
SacI G_AGCT'C
Cuts at: 1168 2115 3553 1168
Size: 947 1438 4154
Fragments arranged by size:
4154 1438 947
SalI G'TCGA_C
Cuts at: 3576 3576
Size: 6539
SanDI GG'GwC_CC
Cuts at: 279 473 584 2429 279
Size: 194 111 1845 4389
Fragments arranged by size:
4389 1845 194 111
SapI GCTCTTCn'nnn_
Cuts at: 1981 3439 4562 4772 1981
Size: 1458 1123 210 3748
Fragments arranged by size:
3748 1458 1123 210
SbfI CC_TGCA'GG
Cuts at: 3586 3586
Size: 6539
ScaI AGT'ACT
Cuts at: 6056 6056
Size: 6539
SfcI C'TryA_G
Cuts at: 33 303 977 1424 2708 3451 3582 4267 5260
Size: 270 674 447 1284 743 131 685 993
Cuts at: 5260 5451 33
Size: 191 1121
Fragments arranged by size:
1284 1121 993 743 685 674 447 270
191 131
SfoI GGC'GCC
Cuts at: 141 1909 3971 4219 141
Size: 1768 2062 248 2461
Fragments arranged by size:
2461 2062 1768 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
SmaI CCC'GGG
Cuts at: 135 814 820 3561 135
Size: 679 6 2741 3113
Fragments arranged by size:
3113 2741 679 6
SmlI C'TyrA_G
Cuts at: 1474 2893 3694 4609 5101 5363 1474
Size: 1419 801 915 492 262 2650
Fragments arranged by size:
2650 1419 915 801 492 262
SnaBI TAC'GTA
Cuts at: 1093 1093
Size: 6539
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6539
SphI G_CATG'C
Cuts at: 3592 4092 3592
Size: 500 6039
SrfI GCCC'GGGC
Cuts at: 820 820
Size: 6539
StuI AGG'CCT
Cuts at: 638 638
Size: 6539
StyI C'CwwG_G
Cuts at: 107 170 230 775 1212 1238 1292 3281 3673
Size: 63 60 545 437 26 54 1989 392
Cuts at: 3673 4027 107
Size: 354 2619
Fragments arranged by size:
2619 1989 545 437 392 354 63 60
54 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 1214 2053 2320 2402 3016 3336 4122 4255
Size: 850 839 267 82 614 320 786 133
Cuts at: 4255 4714 4923 364
Size: 459 209 1980
Fragments arranged by size:
1980 850 839 786 614 459 320 267
209 133 82
TatI w'GTAC_w
Cuts at: 1455 3182 3509 6054 1455
Size: 1727 327 2545 1940
Fragments arranged by size:
2545 1940 1727 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 877 909 6056 6088 491
Size: 32 354 32 5147 32 942
Fragments arranged by size:
5147 942 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4336 4336
Size: 6539
XbaI T'CTAG_A
Cuts at: 3570 3570
Size: 6539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1219 1268 2643 2649 4034 1219
Size: 49 1375 6 1385 3724
Fragments arranged by size:
3724 1385 1375 49 6
XmaI C'CCGG_G
Cuts at: 133 812 818 3559 133
Size: 679 6 2741 3113
Fragments arranged by size:
3113 2741 679 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BcgI
BmtI BsiWI BsmI BsmBI BstZ17I ClaI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SspI SwaI XhoI XmnI ZraI