Restriction map for
pF1KB3863
(Circular) (Six-base) MAPSORT of: pF1KB3863.seq Check: 2389 from: 1 to: 5516
pF1KB3863 5516 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1989 1989
Size: 5516
AccI GT'mk_AC
Cuts at: 435 787 2554 435
Size: 352 1767 3397
Fragments arranged by size:
3397 1767 352
Acc65I G'GTAC_C
Cuts at: 2532 2532
Size: 5516
AclI AA'CG_TT
Cuts at: 5356 5356
Size: 5516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1523 2468 3341 3773 4126 4788 506
Size: 1017 945 873 432 353 662 1234
Fragments arranged by size:
1234 1017 945 873 662 432 353
AfeI AGC'GCT
Cuts at: 719 2209 2433 719
Size: 1490 224 3802
Fragments arranged by size:
3802 1490 224
AflIII A'CryG_T
Cuts at: 2868 4668 4949 2868
Size: 1800 281 3435
Fragments arranged by size:
3435 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 5516
AlwNI CAG_nnn'CTG
Cuts at: 2821 4259 2821
Size: 1438 4078
ApaI G_GGCC'C
Cuts at: 178 178
Size: 5516
ApaLI G'TGCA_C
Cuts at: 4354 4354
Size: 5516
ApoI r'AATT_y
Cuts at: 2520 4869 5125 2520
Size: 2349 256 2911
Fragments arranged by size:
2911 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5516
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5516
AvaI C'yCGr_G
Cuts at: 2536 2536
Size: 5516
AvrII C'CTAG_G
Cuts at: 1834 1834
Size: 5516
BamHI G'GATC_C
Cuts at: 2541 2541
Size: 5516
BanI G'GyrC_C
Cuts at: 258 750 1839 1942 2532 2946 3194 3229 258
Size: 492 1089 103 590 414 248 35 2545
Fragments arranged by size:
2545 1089 590 492 414 248 103 35
BanII G_rGCy'C
Cuts at: 178 244 1129 1576 1659 2129 2530 3560 178
Size: 66 885 447 83 470 401 1030 2134
Fragments arranged by size:
2134 1030 885 470 447 401 83 66
BbeI G_GCGC'C
Cuts at: 2950 3198 2950
Size: 248 5268
BbsI GAAGACnn'nnnn_
Cuts at: 1529 2474 1529
Size: 945 4571
BbvCI CC'TCA_GC
Cuts at: 1294 1294
Size: 5516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5482
BciVI GTATCCnnnnn_n'
Cuts at: 3414 4470 5516 3414
Size: 1056 1046 3414
Fragments arranged by size:
3414 1056 1046
BclI T'GATC_A
Cuts at: 3036 3036
Size: 5516
BglI GCCn_nnn'nGGC
Cuts at: 405 712 988 1916 405
Size: 307 276 928 4005
Fragments arranged by size:
4005 928 307 276
BglII A'GATC_T
Cuts at: 3031 5068 3031
Size: 2037 3479
BlpI GC'TnA_GC
Cuts at: 1665 2056 2628 1665
Size: 391 572 4553
Fragments arranged by size:
4553 572 391
Bme1580I G_kGCm'C
Cuts at: 178 744 1842 3141 3234 4358 178
Size: 566 1098 1299 93 1124 1336
Fragments arranged by size:
1336 1299 1124 1098 566 93
BmrI ACTGGGnnnn_n'
Cuts at: 439 2728 2809 3144 5288 439
Size: 2289 81 335 2144 667
Fragments arranged by size:
2289 2144 667 335 81
BmtI G_CTAG'C
Cuts at: 1010 1010
Size: 5516
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 1593 2415 3883 1206
Size: 387 822 1468 2839
Fragments arranged by size:
2839 1468 822 387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 896 986 1533 1952 2427 706
Size: 190 90 547 419 475 3795
Fragments arranged by size:
3795 547 475 419 190 90
Bpu10I CC'TnA_GC
Cuts at: 1294 2428 1294
Size: 1134 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1045 2164 2224 2692 3571 4285 4583 844
Size: 201 1119 60 468 879 714 298 1777
Fragments arranged by size:
1777 1119 879 714 468 298 201 60
BsaI GGTCTCn'nnnn_
Cuts at: 5220 5220
Size: 5516
BsaAI yAC'GTr
Cuts at: 2137 2230 2869 2137
Size: 93 639 4784
Fragments arranged by size:
4784 639 93
BsaBI GATnn'nnATC
Cuts at: 6 3030 5067 6
Size: 3024 2037 455
Fragments arranged by size:
3024 2037 455
BsaHI Gr'CG_yC
Cuts at: 2947 3195 3897 5393 2947
Size: 248 702 1496 3070
Fragments arranged by size:
3070 1496 702 248
BsaWI w'CCGG_w
Cuts at: 438 2705 3226 3904 4315 4462 4736 438
Size: 2267 521 678 411 147 274 1218
Fragments arranged by size:
2267 1218 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 872 891 902 921 872
Size: 19 11 19 5467
Fragments arranged by size:
5467 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 504 782 899 1566 1603 1606 1618 1627 2023
Size: 278 117 667 37 3 12 9 396
Cuts at: 2023 2026 2451 2454 504
Size: 3 425 3 3566
Fragments arranged by size:
3566 667 425 396 278 117 37 12
9 3 3 3
BseYI C'CCAG_C
Cuts at: 165 171 917 1807 2091 2941 3699 4364 4772
Size: 6 746 890 284 850 758 665 408
Cuts at: 4772 165
Size: 909
Fragments arranged by size:
909 890 850 758 746 665 408 284
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 2387 1229
Size: 1158 4358
BsiEI CG_ry'CG
Cuts at: 104 3058 3104 4334 104
Size: 2954 46 1230 1286
Fragments arranged by size:
2954 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1129 1576 2530 3308 3498 4358 1129
Size: 447 954 778 190 860 2287
Fragments arranged by size:
2287 954 860 778 447 190
BsmI GAATG_Cn'
Cuts at: 159 159
Size: 5516
BsmBI CGTCTCn'nnnn_
Cuts at: 765 765
Size: 5516
Bsp1286I G_dGCh'C
Cuts at: 178 244 744 1129 1576 1659 1842 2129 2530
Size: 66 500 385 447 83 183 287 401
Cuts at: 2530 3141 3234 3308 3498 3560 4358 178
Size: 611 93 74 190 62 798 1336
Fragments arranged by size:
1336 798 611 500 447 401 385 287
190 183 93 83 74 66 62
BspHI T'CATG_A
Cuts at: 3948 3948
Size: 5516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1989 2566 2780 3082 3463 1989
Size: 577 214 302 381 4042
Fragments arranged by size:
4042 577 381 302 214
BsrBI CCG'CTC
Cuts at: 50 888 3808 3862 5346 5511 50
Size: 838 2920 54 1484 165 55
Fragments arranged by size:
2920 1484 838 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1161 1173 1424 3428 1161
Size: 12 251 2004 3249
Fragments arranged by size:
3249 2004 251 12
BsrFI r'CCGG_y
Cuts at: 3514 3695 3514
Size: 181 5335
BssSI C'ACGA_G
Cuts at: 3787 4495 3787
Size: 708 4808
BstAPI GCAn_nnn'nTGC
Cuts at: 2893 2893
Size: 5516
BstBI TT'CG_AA
Cuts at: 207 3877 207
Size: 3670 1846
BstYI r'GATC_y
Cuts at: 1 2541 3008 3031 3366 3612 3918 3930 4016
Size: 2540 467 23 335 246 306 12 86
Cuts at: 4016 5068 1
Size: 1052 449
Fragments arranged by size:
2540 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 788 788
Size: 5516
Bsu36I CC'TnA_GG
Cuts at: 1031 1031
Size: 5516
BtgI C'CryG_G
Cuts at: 1286 1286
Size: 5516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 3466 3647 3810 1645
Size: 1821 181 163 3351
Fragments arranged by size:
3351 1821 181 163
BtsI GCAGTG_nn'
Cuts at: 735 1572 1890 1902 2423 2759 735
Size: 837 318 12 521 336 3492
Fragments arranged by size:
3492 837 521 336 318 12
DraI TTT'AAA
Cuts at: 2515 2515
Size: 5516
DrdI GACnn_nn'nnGTC
Cuts at: 3222 4566 3222
Size: 1344 4172
EaeI y'GGCC_r
Cuts at: 397 1526 3101 3275 3666 3693 5038 397
Size: 1129 1575 174 391 27 1345 875
Fragments arranged by size:
1575 1345 1129 875 391 174 27
EagI C'GGCC_G
Cuts at: 3101 3101
Size: 5516
EarI CTCTTCn'nnn_
Cuts at: 193 768 1580 1616 2012 2147 2472 3539 3749
Size: 575 812 36 396 135 325 1067 210
Cuts at: 3749 193
Size: 1960
Fragments arranged by size:
1960 1067 812 575 396 325 210 135
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3724 4466 4612 5326 3724
Size: 742 146 714 3914
Fragments arranged by size:
3914 742 714 146
EcoICRI GAG'CTC
Cuts at: 1127 1574 2528 1127
Size: 447 954 4115
Fragments arranged by size:
4115 954 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 706 896 986 1523 1533 1952 2427 2468
Size: 200 190 90 537 10 419 475 41
Cuts at: 2468 3341 3773 4126 4788 506
Size: 873 432 353 662 1234
Fragments arranged by size:
1234 873 662 537 475 432 419 353
200 190 90 41 10
EcoNI CCTnn'n_nnAGG
Cuts at: 146 355 631 1931 146
Size: 209 276 1300 3731
Fragments arranged by size:
3731 1300 276 209
EcoO109I rG'GnC_Cy
Cuts at: 1489 1831 1909 2655 1489
Size: 342 78 746 4350
Fragments arranged by size:
4350 746 342 78
EcoRI G'AATT_C
Cuts at: 2520 2520
Size: 5516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1159 3535 1159
Size: 2376 3140
FspI TGC'GCA
Cuts at: 3297 3297
Size: 5516
HaeII r_GCGC'y
Cuts at: 721 2211 2435 2950 3198 4428 5199 721
Size: 1490 224 515 248 1230 771 1038
Fragments arranged by size:
1490 1230 1038 771 515 248 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 872 904 2443 2475 2488 2520 3358
Size: 32 483 32 1539 32 13 32 838
Cuts at: 3358 3388 3390 3420 357
Size: 30 2 30 2453
Fragments arranged by size:
2453 1539 838 483 32 32 32 32
30 30 13 2
HincII GTy'rAC
Cuts at: 2555 2555
Size: 5516
HindIII A'AGCT_T
Cuts at: 1141 1141
Size: 5516
KasI G'GCGC_C
Cuts at: 2946 3194 2946
Size: 248 5268
KpnI G_GTAC'C
Cuts at: 2536 2536
Size: 5516
MluI A'CGCG_T
Cuts at: 4949 4949
Size: 5516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1400 4275 4459 1400
Size: 2875 184 2457
Fragments arranged by size:
2875 2457 184
MscI TGG'CCA
Cuts at: 399 1528 3277 399
Size: 1129 1749 2638
Fragments arranged by size:
2638 1749 1129
MslI CAynn'nnrTG
Cuts at: 1166 3505 3632 1166
Size: 2339 127 3050
Fragments arranged by size:
3050 2339 127
MspA1I CmG'CkG
Cuts at: 342 2055 2339 2627 2941 3301 4085 4330 342
Size: 1713 284 288 314 360 784 245 1528
Fragments arranged by size:
1713 1528 784 360 314 288 284 245
NaeI GCC'GGC
Cuts at: 3697 3697
Size: 5516
NarI GG'CG_CC
Cuts at: 2947 3195 2947
Size: 248 5268
NcoI C'CATG_G
Cuts at: 1286 1286
Size: 5516
NgoMIV G'CCGG_C
Cuts at: 3695 3695
Size: 5516
NheI G'CTAG_C
Cuts at: 1006 1006
Size: 5516
NspI r_CATG'y
Cuts at: 1204 2569 3069 4672 1204
Size: 1365 500 1603 2048
Fragments arranged by size:
2048 1603 1365 500
PasI CC'CwG_GG
Cuts at: 142 1722 142
Size: 1580 3936
PciI A'CATG_T
Cuts at: 4668 4668
Size: 5516
PmeI GTTT'AAAC
Cuts at: 2515 2515
Size: 5516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 5484
PsiI TTA'TAA
Cuts at: 457 457
Size: 5516
PspOMI G'GGCC_C
Cuts at: 174 174
Size: 5516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1312 1344 1312
Size: 32 5484
PstI C_TGCA'G
Cuts at: 906 2563 3248 906
Size: 1657 685 3174
Fragments arranged by size:
3174 1657 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5516
PvuII CAG'CTG
Cuts at: 342 2055 2941 3301 342
Size: 1713 886 360 2557
Fragments arranged by size:
2557 1713 886 360
RsrII CG'GwC_CG
Cuts at: 3711 3711
Size: 5516
SacI G_AGCT'C
Cuts at: 1129 1576 2530 1129
Size: 447 954 4115
Fragments arranged by size:
4115 954 447
SalI G'TCGA_C
Cuts at: 2553 2553
Size: 5516
SapI GCTCTTCn'nnn_
Cuts at: 3539 3749 3539
Size: 210 5306
SbfI CC_TGCA'GG
Cuts at: 2563 2563
Size: 5516
ScaI AGT'ACT
Cuts at: 5033 5033
Size: 5516
SfcI C'TryA_G
Cuts at: 33 628 902 977 1432 1675 1991 2559 3244
Size: 595 274 75 455 243 316 568 685
Cuts at: 3244 4212 4403 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 595 568 455 316 274
243 191 75
SfoI GGC'GCC
Cuts at: 2948 3196 2948
Size: 248 5268
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5516
SmaI CCC'GGG
Cuts at: 2538 2538
Size: 5516
SmlI C'TyrA_G
Cuts at: 859 1024 2179 2203 2671 3586 4300 4562 859
Size: 165 1155 24 468 915 714 262 1813
Fragments arranged by size:
1813 1155 915 714 468 262 165 24
SnaBI TAC'GTA
Cuts at: 2230 2230
Size: 5516
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5516
SphI G_CATG'C
Cuts at: 1204 2569 3069 1204
Size: 1365 500 3651
Fragments arranged by size:
3651 1365 500
StyI C'CwwG_G
Cuts at: 1286 1834 2650 3004 1286
Size: 548 816 354 3798
Fragments arranged by size:
3798 816 548 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 848 3099 3232 3691 3900 848
Size: 2251 133 459 209 2464
Fragments arranged by size:
2464 2251 459 209 133
TatI w'GTAC_w
Cuts at: 1388 1695 5031 1388
Size: 307 3336 1873
Fragments arranged by size:
3336 1873 307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5033 5065 5033
Size: 32 5484
Tth111I GACn'n_nGTC
Cuts at: 3313 3313
Size: 5516
XbaI T'CTAG_A
Cuts at: 2547 2547
Size: 5516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3011 3011
Size: 5516
XmaI C'CCGG_G
Cuts at: 2536 2536
Size: 5516
XmnI GAAnn'nnTTC
Cuts at: 1143 1685 1143
Size: 542 4974
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PmeI
PpiI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AloI AscI BaeI
BfrBI BmgBI BsiWI BspEI BsrGI BssHII BstEII BstXI
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI