Restriction map for
pF1KB3860
(Circular) (Six-base) MAPSORT of: pF1KB3860.seq Check: 9323 from: 1 to: 4157
pF1KB3860 4157 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 652 836 652
Size: 184 3973
AccI GT'mk_AC
Cuts at: 257 1195 257
Size: 938 3219
Acc65I G'GTAC_C
Cuts at: 224 1173 224
Size: 949 3208
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2414 2767 3429 1982
Size: 432 353 662 2710
Fragments arranged by size:
2710 662 432 353
AfeI AGC'GCT
Cuts at: 1030 1030
Size: 4157
AflIII A'CryG_T
Cuts at: 146 680 800 1509 3309 3590 146
Size: 534 120 709 1800 281 713
Fragments arranged by size:
1800 713 709 534 281 120
AlwNI CAG_nnn'CTG
Cuts at: 322 358 667 1462 2900 322
Size: 36 309 795 1438 1579
Fragments arranged by size:
1579 1438 795 309 36
ApaI G_GGCC'C
Cuts at: 248 248
Size: 4157
ApaLI G'TGCA_C
Cuts at: 2995 2995
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AscI GG'CGCG_CC
Cuts at: 203 203
Size: 4157
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 1177 1177
Size: 4157
BamHI G'GATC_C
Cuts at: 589 1182 589
Size: 593 3564
BanI G'GyrC_C
Cuts at: 224 631 1099 1173 1587 1835 1870 224
Size: 407 468 74 414 248 35 2511
Fragments arranged by size:
2511 468 414 407 248 74 35
BanII G_rGCy'C
Cuts at: 248 308 1171 2201 248
Size: 60 863 1030 2204
Fragments arranged by size:
2204 1030 863 60
BbeI G_GCGC'C
Cuts at: 1103 1591 1839 1103
Size: 488 248 3421
Fragments arranged by size:
3421 488 248
BbvCI CC'TCA_GC
Cuts at: 229 229
Size: 4157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 180 214 942 976 90
Size: 34 56 34 728 34 3271
Fragments arranged by size:
3271 728 56 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2055 3111 4157 2055
Size: 1056 1046 2055
Fragments arranged by size:
2055 1056 1046
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 532 611 532
Size: 79 4078
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1135 1269 1135
Size: 134 4023
Bme1580I G_kGCm'C
Cuts at: 248 1782 1875 2999 248
Size: 1534 93 1124 1406
Fragments arranged by size:
1534 1406 1124 93
BmgBI CAC'GTC
Cuts at: 713 785 713
Size: 72 4085
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 4157
Bpu10I CC'TnA_GC
Cuts at: 229 724 229
Size: 495 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1333 2212 2926 3224 1036
Size: 297 879 714 298 1969
Fragments arranged by size:
1969 879 714 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 240 801 1510 240
Size: 561 709 2887
Fragments arranged by size:
2887 709 561
BsaBI GATnn'nnATC
Cuts at: 6 1671 3708 6
Size: 1665 2037 455
Fragments arranged by size:
2037 1665 455
BsaHI Gr'CG_yC
Cuts at: 269 452 551 1100 1588 1836 2538 4034 269
Size: 183 99 549 488 248 702 1496 392
Fragments arranged by size:
1496 702 549 488 392 248 183 99
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2956 3103 3377 1346
Size: 521 678 411 147 274 2126
Fragments arranged by size:
2126 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 295
Size: 30 4127
BseYI C'CCAG_C
Cuts at: 336 1582 2340 3005 3413 336
Size: 1246 758 665 408 1080
Fragments arranged by size:
1246 1080 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1165 196
Size: 969 3188
BsiEI CG_ry'CG
Cuts at: 104 161 428 675 969 1699 1745 2975 104
Size: 57 267 247 294 730 46 1230 1286
Fragments arranged by size:
1286 1230 730 294 267 247 57 46
BsiHKAI G_wGCw'C
Cuts at: 1171 1949 2139 2999 1171
Size: 778 190 860 2329
Fragments arranged by size:
2329 860 778 190
BsmI GAATG_Cn'
Cuts at: 999 999
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 248 308 1171 1782 1875 1949 2139 2201 2999
Size: 60 863 611 93 74 190 62 798
Cuts at: 2999 248
Size: 1406
Fragments arranged by size:
1406 863 798 611 190 93 74 62
60
BspHI T'CATG_A
Cuts at: 2589 2589
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 836 851 986 1207 1421 1723 2104 652
Size: 184 15 135 221 214 302 381 2705
Fragments arranged by size:
2705 381 302 221 214 184 135 15
BsrBI CCG'CTC
Cuts at: 50 514 971 2449 2503 3987 4152 50
Size: 464 457 1478 54 1484 165 55
Fragments arranged by size:
1484 1478 464 457 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2069 2069
Size: 4157
BsrFI r'CCGG_y
Cuts at: 499 674 810 919 2155 2336 499
Size: 175 136 109 1236 181 2320
Fragments arranged by size:
2320 1236 181 175 136 109
BssHII G'CGCG_C
Cuts at: 203 618 640 642 941 981 983 203
Size: 415 22 2 299 40 2 3377
Fragments arranged by size:
3377 415 299 40 22 2 2
BssSI C'ACGA_G
Cuts at: 2428 3136 2428
Size: 708 3449
BstAPI GCAn_nnn'nTGC
Cuts at: 358 843 1031 1534 358
Size: 485 188 503 2981
Fragments arranged by size:
2981 503 485 188
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstYI r'GATC_y
Cuts at: 1 589 1182 1649 1672 2007 2253 2559 2571
Size: 588 593 467 23 335 246 306 12
Cuts at: 2571 2657 3709 1
Size: 86 1052 449
Fragments arranged by size:
1052 593 588 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 815 1112 815
Size: 297 3860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 208 945 1400 208
Size: 737 455 2965
Fragments arranged by size:
2965 737 455
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 427 1863 3207 427
Size: 1436 1344 1377
Fragments arranged by size:
1436 1377 1344
EaeI y'GGCC_r
Cuts at: 158 389 425 672 812 818 1742 1916 2307
Size: 231 36 247 140 6 924 174 391
Cuts at: 2307 2334 3679 158
Size: 27 1345 636
Fragments arranged by size:
1345 924 636 391 247 231 174 140
36 27 6
EagI C'GGCC_G
Cuts at: 158 425 672 1742 158
Size: 267 247 1070 2573
Fragments arranged by size:
2573 1070 267 247
EarI CTCTTCn'nnn_
Cuts at: 159 1033 2180 2390 159
Size: 874 1147 210 1926
Fragments arranged by size:
1926 1147 874 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 463 2365 3107 3253 3967 463
Size: 1902 742 146 714 653
Fragments arranged by size:
1902 742 714 653 146
EcoICRI GAG'CTC
Cuts at: 1169 1169
Size: 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1982 2414 2767 3429 187
Size: 1795 432 353 662 915
Fragments arranged by size:
1795 915 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 116 244 245 1108 1296 116
Size: 128 1 863 188 2977
Fragments arranged by size:
2977 863 188 128 1
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1059 2176 1059
Size: 1117 3040
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 176 477 777 1032 1103 1591 1839 3069 3840
Size: 301 300 255 71 488 248 1230 771
Cuts at: 3840 176
Size: 493
Fragments arranged by size:
1230 771 493 488 301 300 255 248
71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 460 492 1999 2029 2031 2061 136
Size: 32 292 32 1507 30 2 30 2232
Fragments arranged by size:
2232 1507 292 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1196 1196
Size: 4157
KasI G'GCGC_C
Cuts at: 1099 1587 1835 1099
Size: 488 248 3421
Fragments arranged by size:
3421 488 248
KpnI G_GTAC'C
Cuts at: 228 1177 228
Size: 949 3208
MluI A'CGCG_T
Cuts at: 146 680 3590 146
Size: 534 2910 713
Fragments arranged by size:
2910 713 534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 998 2916 3100 998
Size: 1918 184 2055
Fragments arranged by size:
2055 1918 184
MscI TGG'CCA
Cuts at: 391 820 1918 391
Size: 429 1098 2630
Fragments arranged by size:
2630 1098 429
MslI CAynn'nnrTG
Cuts at: 820 2146 2273 820
Size: 1326 127 2704
Fragments arranged by size:
2704 1326 127
MspA1I CmG'CkG
Cuts at: 322 397 765 1114 1268 1582 1942 2726 2971
Size: 75 368 349 154 314 360 784 245
Cuts at: 2971 322
Size: 1508
Fragments arranged by size:
1508 784 368 360 349 314 245 154
75
NaeI GCC'GGC
Cuts at: 501 676 812 2338 501
Size: 175 136 1526 2320
Fragments arranged by size:
2320 1526 175 136
NarI GG'CG_CC
Cuts at: 1100 1588 1836 1100
Size: 488 248 3421
Fragments arranged by size:
3421 488 248
NcoI C'CATG_G
Cuts at: 815 815
Size: 4157
NgoMIV G'CCGG_C
Cuts at: 499 674 810 2336 499
Size: 175 136 1526 2320
Fragments arranged by size:
2320 1526 175 136
NspI r_CATG'y
Cuts at: 909 993 1210 1710 3313 909
Size: 84 217 500 1603 1753
Fragments arranged by size:
1753 1603 500 217 84
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 4125
PpuMI rG'GwC_Cy
Cuts at: 116 116
Size: 4157
PspOMI G'GGCC_C
Cuts at: 244 244
Size: 4157
PstI C_TGCA'G
Cuts at: 354 899 1204 1889 354
Size: 545 305 685 2622
Fragments arranged by size:
2622 685 545 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 322 765 1582 1942 322
Size: 443 817 360 2537
Fragments arranged by size:
2537 817 443 360
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 1171 1171
Size: 4157
SacII CC_GC'GG
Cuts at: 1115 1115
Size: 4157
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 1033 2180 2390 1033
Size: 1147 210 2800
Fragments arranged by size:
2800 1147 210
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 350 895 1200 1885 2853 3044 33
Size: 317 545 305 685 968 191 1146
Fragments arranged by size:
1146 968 685 545 317 305 191
SfoI GGC'GCC
Cuts at: 1101 1589 1837 1101
Size: 488 248 3421
Fragments arranged by size:
3421 488 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SgrAI Cr'CCGG_yG
Cuts at: 919 919
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 1051 1312 2227 2941 3203 1051
Size: 261 915 714 262 2005
Fragments arranged by size:
2005 915 714 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StuI AGG'CCT
Cuts at: 958 958
Size: 4157
StyI C'CwwG_G
Cuts at: 815 1291 1645 815
Size: 476 354 3327
Fragments arranged by size:
3327 476 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1740 1873 2332 2541 1740
Size: 133 459 209 3356
Fragments arranged by size:
3356 459 209 133
TatI w'GTAC_w
Cuts at: 3672 3672
Size: 4157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 813 1652 120
Size: 693 839 2625
Fragments arranged by size:
2625 839 693
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
XmnI GAAnn'nnTTC
Cuts at: 931 1042 931
Size: 111 4046
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI AvrII
BaeI BbsI BfrBI BmtI BseRI BsiWI BsmBI BspEI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI