Restriction map for
pF1KB3849
(Circular) (Six-base) MAPSORT of: pF1KB3849.seq Check: 7374 from: 1 to: 5882
pF1KB3849 5882 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2821 2821
Size: 5882
AatII G_ACGT'C
Cuts at: 1430 1430
Size: 5882
AccI GT'mk_AC
Cuts at: 2485 2765 2920 2485
Size: 280 155 5447
Fragments arranged by size:
5447 280 155
Acc65I G'GTAC_C
Cuts at: 2898 2898
Size: 5882
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1929 3707 4139 4492 5154 1929
Size: 1778 432 353 662 2657
Fragments arranged by size:
2657 1778 662 432 353
AflIII A'CryG_T
Cuts at: 842 1607 3234 5034 5315 842
Size: 765 1627 1800 281 1409
Fragments arranged by size:
1800 1627 1409 765 281
AleI CACnn'nnGTG
Cuts at: 886 886
Size: 5882
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 2294 2326 256
Size: 32 2006 32 3812
Fragments arranged by size:
3812 2006 32 32
AlwNI CAG_nnn'CTG
Cuts at: 907 2019 3187 4625 907
Size: 1112 1168 1438 2164
Fragments arranged by size:
2164 1438 1168 1112
ApaLI G'TGCA_C
Cuts at: 4720 4720
Size: 5882
ApoI r'AATT_y
Cuts at: 421 502 513 978 1293 2886 5235 5491 421
Size: 81 11 465 315 1593 2349 256 812
Fragments arranged by size:
2349 1593 812 465 315 256 81 11
AseI AT'TA_AT
Cuts at: 20 485 646 1100 20
Size: 465 161 454 4802
Fragments arranged by size:
4802 465 454 161
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 220 1936 2902 220
Size: 1716 966 3200
Fragments arranged by size:
3200 1716 966
BamHI G'GATC_C
Cuts at: 794 2907 794
Size: 2113 3769
BanI G'GyrC_C
Cuts at: 118 778 880 1176 1827 2554 2587 2686 2692
Size: 660 102 296 651 727 33 99 6
Cuts at: 2692 2898 3312 3560 3595 118
Size: 206 414 248 35 2405
Fragments arranged by size:
2405 727 660 651 414 296 248 206
102 99 35 33 6
BanII G_rGCy'C
Cuts at: 241 791 1711 1847 1937 1943 2671 2896 3926
Size: 550 920 136 90 6 728 225 1030
Cuts at: 3926 241
Size: 2197
Fragments arranged by size:
2197 1030 920 728 550 225 136 90
6
BbeI G_GCGC'C
Cuts at: 122 3316 3564 122
Size: 3194 248 2440
Fragments arranged by size:
3194 2440 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1792 1826 1792
Size: 34 5848
BciVI GTATCCnnnnn_n'
Cuts at: 3780 4836 5882 3780
Size: 1056 1046 3780
Fragments arranged by size:
3780 1056 1046
BclI T'GATC_A
Cuts at: 1199 3402 1199
Size: 2203 3679
BglII A'GATC_T
Cuts at: 3397 5434 3397
Size: 2037 3845
BlpI GC'TnA_GC
Cuts at: 1720 2153 2994 1720
Size: 433 841 4608
Fragments arranged by size:
4608 841 433
Bme1580I G_kGCm'C
Cuts at: 2747 3507 3600 4724 2747
Size: 760 93 1124 3905
Fragments arranged by size:
3905 1124 760 93
BmgBI CAC'GTC
Cuts at: 843 843
Size: 5882
BmrI ACTGGGnnnn_n'
Cuts at: 2718 3094 3175 3510 5654 2718
Size: 376 81 335 2144 2946
Fragments arranged by size:
2946 2144 376 335 81
BmtI G_CTAG'C
Cuts at: 1931 1931
Size: 5882
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4249 4249
Size: 5882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1380 2235 2286 715
Size: 665 855 51 4311
Fragments arranged by size:
4311 855 665 51
Bpu10I CC'TnA_GC
Cuts at: 772 772
Size: 5882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 3937 4651 4949 3058
Size: 879 714 298 3991
Fragments arranged by size:
3991 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1021 2290 2541 5586 1021
Size: 1269 251 3045 1317
Fragments arranged by size:
3045 1317 1269 251
BsaAI yAC'GTr
Cuts at: 462 1608 1917 2742 3235 462
Size: 1146 309 825 493 3109
Fragments arranged by size:
3109 1146 825 493 309
BsaBI GATnn'nnATC
Cuts at: 6 3396 5433 6
Size: 3390 2037 455
Fragments arranged by size:
3390 2037 455
BsaHI Gr'CG_yC
Cuts at: 119 809 1427 1459 2641 3313 3561 4263 5759
Size: 690 618 32 1182 672 248 702 1496
Cuts at: 5759 119
Size: 242
Fragments arranged by size:
1496 1182 702 690 672 618 248 242
32
BsaWI w'CCGG_w
Cuts at: 791 1443 3071 3592 4270 4681 4828 5102 791
Size: 652 1628 521 678 411 147 274 1571
Fragments arranged by size:
1628 1571 678 652 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1050 1080 1050
Size: 30 5852
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 1383 2238 400
Size: 983 855 4044
Fragments arranged by size:
4044 983 855
BseYI C'CCAG_C
Cuts at: 129 1794 2826 3307 4065 4730 5138 129
Size: 1665 1032 481 758 665 408 873
Fragments arranged by size:
1665 1032 873 758 665 481 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 2059 527
Size: 1532 4350
BsiEI CG_ry'CG
Cuts at: 104 1758 3424 3470 4700 104
Size: 1654 1666 46 1230 1286
Fragments arranged by size:
1666 1654 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 158 417 791 2201 2671 2896 3674 3864 4724
Size: 259 374 1410 470 225 778 190 860
Cuts at: 4724 158
Size: 1316
Fragments arranged by size:
1410 1316 860 778 470 374 259 225
190
BsmBI CGTCTCn'nnnn_
Cuts at: 833 1418 833
Size: 585 5297
Bsp1286I G_dGCh'C
Cuts at: 158 241 417 791 1711 1847 1937 1943 2201
Size: 83 176 374 920 136 90 6 258
Cuts at: 2201 2671 2747 2896 3507 3600 3674 3864 3926
Size: 470 76 149 611 93 74 190 62
Cuts at: 3926 4724 158
Size: 798 1316
Fragments arranged by size:
1316 920 798 611 470 374 258 190
176 149 136 93 90 83 76 74
62 6
BspEI T'CCGG_A
Cuts at: 791 791
Size: 5882
BspHI T'CATG_A
Cuts at: 4314 4314
Size: 5882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2821 2932 3146 3448 3829 2821
Size: 111 214 302 381 4874
Fragments arranged by size:
4874 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 116 350 989 4174 4228 5712 5877 50
Size: 66 234 639 3185 54 1484 165 55
Fragments arranged by size:
3185 1484 639 234 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 2787 2844 3794 2787
Size: 57 950 4875
Fragments arranged by size:
4875 950 57
BsrFI r'CCGG_y
Cuts at: 2584 2689 3880 4061 2584
Size: 105 1191 181 4405
Fragments arranged by size:
4405 1191 181 105
BsrGI T'GTAC_A
Cuts at: 845 1087 845
Size: 242 5640
BssHII G'CGCG_C
Cuts at: 1619 1647 1833 1619
Size: 28 186 5668
Fragments arranged by size:
5668 186 28
BssSI C'ACGA_G
Cuts at: 4153 4861 4153
Size: 708 5174
BstAPI GCAn_nnn'nTGC
Cuts at: 583 2106 2742 3259 583
Size: 1523 636 517 3206
Fragments arranged by size:
3206 1523 636 517
BstBI TT'CG_AA
Cuts at: 1679 4243 1679
Size: 2564 3318
BstEII G'GTnAC_C
Cuts at: 801 1857 801
Size: 1056 4826
BstXI CCAn_nnnn'nTGG
Cuts at: 267 1222 1869 2637 267
Size: 955 647 768 3512
Fragments arranged by size:
3512 955 768 647
BstYI r'GATC_y
Cuts at: 1 203 794 2907 3374 3397 3732 3978 4284
Size: 202 591 2113 467 23 335 246 306
Cuts at: 4284 4296 4382 5434 1
Size: 12 86 1052 449
Fragments arranged by size:
2113 1052 591 467 449 335 306 246
202 86 23 12
BtgI C'CryG_G
Cuts at: 107 260 1774 1874 2696 107
Size: 153 1514 100 822 3293
Fragments arranged by size:
3293 1514 822 153 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1491 2591 3832 4013 4176 1491
Size: 1100 1241 181 163 3197
Fragments arranged by size:
3197 1241 1100 181 163
BtsI GCAGTG_nn'
Cuts at: 3125 3125
Size: 5882
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DrdI GACnn_nn'nnGTC
Cuts at: 3588 4932 3588
Size: 1344 4538
EaeI y'GGCC_r
Cuts at: 1548 1571 1871 2210 2222 3467 3641 4032 4059
Size: 23 300 339 12 1245 174 391 27
Cuts at: 4059 5404 1548
Size: 1345 2026
Fragments arranged by size:
2026 1345 1245 391 339 300 174 27
23 12
EagI C'GGCC_G
Cuts at: 3467 3467
Size: 5882
EarI CTCTTCn'nnn_
Cuts at: 169 1019 1287 1822 1948 2206 3905 4115 169
Size: 850 268 535 126 258 1699 210 1936
Fragments arranged by size:
1936 1699 850 535 268 258 210 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2049 2634 4090 4832 4978 5692 2049
Size: 585 1456 742 146 714 2239
Fragments arranged by size:
2239 1456 742 714 585 146
EcoICRI GAG'CTC
Cuts at: 789 2669 2894 789
Size: 1880 225 3777
Fragments arranged by size:
3777 1880 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1380 1929 2235 2286 3707 4139 4492 5154
Size: 665 549 306 51 1421 432 353 662
Cuts at: 5154 715
Size: 1443
Fragments arranged by size:
1443 1421 665 662 549 432 353 306
51
EcoO109I rG'GnC_Cy
Cuts at: 1499 1798 2453 2823 3021 1499
Size: 299 655 370 198 4360
Fragments arranged by size:
4360 655 370 299 198
EcoRI G'AATT_C
Cuts at: 978 2886 978
Size: 1908 3974
EcoRV GAT'ATC
Cuts at: 2118 2118
Size: 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 2449 3901 345
Size: 2104 1452 2326
Fragments arranged by size:
2326 2104 1452
FspI TGC'GCA
Cuts at: 1672 1960 1975 3663 1672
Size: 288 15 1688 3891
Fragments arranged by size:
3891 1688 288 15
FspAI rTGC'GCAy
Cuts at: 1975 1975
Size: 5882
HaeII r_GCGC'y
Cuts at: 122 193 2019 2711 3316 3564 4794 5565 122
Size: 71 1826 692 605 248 1230 771 439
Fragments arranged by size:
1826 1230 771 692 605 439 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 714 746 1693 1725 1870 1902 2478 2510 2653
Size: 32 947 32 145 32 576 32 143
Cuts at: 2653 2685 3724 3754 3756 3786 714
Size: 32 1039 30 2 30 2810
Fragments arranged by size:
2810 1039 947 576 145 143 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1431 2486 2921 1431
Size: 1055 435 4392
Fragments arranged by size:
4392 1055 435
KasI G'GCGC_C
Cuts at: 118 3312 3560 118
Size: 3194 248 2440
Fragments arranged by size:
3194 2440 248
KpnI G_GTAC'C
Cuts at: 2902 2902
Size: 5882
MfeI C'AATT_G
Cuts at: 2410 2410
Size: 5882
MluI A'CGCG_T
Cuts at: 5315 5315
Size: 5882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2301 4641 4825 2301
Size: 2340 184 3358
Fragments arranged by size:
3358 2340 184
MscI TGG'CCA
Cuts at: 1573 3643 1573
Size: 2070 3812
MslI CAynn'nnrTG
Cuts at: 464 886 2617 3871 3998 464
Size: 422 1731 1254 127 2348
Fragments arranged by size:
2348 1731 1254 422 127
MspA1I CmG'CkG
Cuts at: 1668 1776 2561 2803 2993 3307 3667 4451 4696
Size: 108 785 242 190 314 360 784 245
Cuts at: 4696 1668
Size: 2854
Fragments arranged by size:
2854 785 784 360 314 245 242 190
108
NaeI GCC'GGC
Cuts at: 2691 4063 2691
Size: 1372 4510
NarI GG'CG_CC
Cuts at: 119 3313 3561 119
Size: 3194 248 2440
Fragments arranged by size:
3194 2440 248
NcoI C'CATG_G
Cuts at: 107 2696 107
Size: 2589 3293
NgoMIV G'CCGG_C
Cuts at: 2689 4061 2689
Size: 1372 4510
NheI G'CTAG_C
Cuts at: 1927 1927
Size: 5882
NspI r_CATG'y
Cuts at: 2935 3435 5038 2935
Size: 500 1603 3779
Fragments arranged by size:
3779 1603 500
PasI CC'CwG_GG
Cuts at: 2676 2676
Size: 5882
PciI A'CATG_T
Cuts at: 5034 5034
Size: 5882
PflMI CCAn_nnn'nTGG
Cuts at: 2833 2833
Size: 5882
PfoI T'CCnGG_A
Cuts at: 281 281
Size: 5882
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PmlI CAC'GTG
Cuts at: 462 1917 2742 462
Size: 1455 825 3602
Fragments arranged by size:
3602 1455 825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 4328
Size: 32 5850
PpuMI rG'GwC_Cy
Cuts at: 1798 1798
Size: 5882
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1673 1705 1673
Size: 32 5850
PstI C_TGCA'G
Cuts at: 611 1842 1925 2162 2738 2929 3614 611
Size: 1231 83 237 576 191 685 2879
Fragments arranged by size:
2879 1231 685 576 237 191 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5882
PvuII CAG'CTG
Cuts at: 1668 2803 3307 3667 1668
Size: 1135 504 360 3883
Fragments arranged by size:
3883 1135 504 360
RsrII CG'GwC_CG
Cuts at: 4077 4077
Size: 5882
SacI G_AGCT'C
Cuts at: 791 2671 2896 791
Size: 1880 225 3777
Fragments arranged by size:
3777 1880 225
SacII CC_GC'GG
Cuts at: 1777 1777
Size: 5882
SalI G'TCGA_C
Cuts at: 2484 2919 2484
Size: 435 5447
SanDI GG'GwC_CC
Cuts at: 1798 1798
Size: 5882
SapI GCTCTTCn'nnn_
Cuts at: 1822 1948 2206 3905 4115 1822
Size: 126 258 1699 210 3589
Fragments arranged by size:
3589 1699 258 210 126
SbfI CC_TGCA'GG
Cuts at: 2929 2929
Size: 5882
ScaI AGT'ACT
Cuts at: 5399 5399
Size: 5882
SexAI A'CCwGG_T
Cuts at: 1853 2280 1853
Size: 427 5455
SfcI C'TryA_G
Cuts at: 33 210 607 951 1690 1838 1921 2158 2322
Size: 177 397 344 739 148 83 237 164
Cuts at: 2322 2734 2925 3610 4578 4769 33
Size: 412 191 685 968 191 1146
Fragments arranged by size:
1146 968 739 685 412 397 344 237
191 191 177 164 148 83
SfoI GGC'GCC
Cuts at: 120 3314 3562 120
Size: 3194 248 2440
Fragments arranged by size:
3194 2440 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SmaI CCC'GGG
Cuts at: 1938 2904 1938
Size: 966 4916
SmlI C'TyrA_G
Cuts at: 3037 3952 4666 4928 3037
Size: 915 714 262 3991
Fragments arranged by size:
3991 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5882
SphI G_CATG'C
Cuts at: 2935 3435 2935
Size: 500 5382
SrfI GCCC'GGGC
Cuts at: 1938 1938
Size: 5882
SspI AAT'ATT
Cuts at: 1039 1104 1039
Size: 65 5817
StyI C'CwwG_G
Cuts at: 107 233 749 1031 1862 1988 2180 2696 3016
Size: 126 516 282 831 126 192 516 320
Cuts at: 3016 3370 107
Size: 354 2619
Fragments arranged by size:
2619 831 516 516 354 320 282 192
126 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1343 1772 3465 3598 4057 4266 1343
Size: 429 1693 133 459 209 2959
Fragments arranged by size:
2959 1693 459 429 209 133
TatI w'GTAC_w
Cuts at: 845 1087 1346 5397 845
Size: 242 259 4051 1330
Fragments arranged by size:
4051 1330 259 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1050 1082 1444 1476 5399 5431 1050
Size: 32 362 32 3923 32 1501
Fragments arranged by size:
3923 1501 362 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3679 3679
Size: 5882
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2076 3377 2076
Size: 1301 4581
XmaI C'CCGG_G
Cuts at: 1936 2902 1936
Size: 966 4916
XmnI GAAnn'nnTTC
Cuts at: 1160 1160
Size: 5882
ZraI GAC'GTC
Cuts at: 1428 1428
Size: 5882
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BsiWI BsmI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI HindIII HpaI
NdeI NotI NruI NsiI PacI PshAI PsiI PspOMI
PspXI SfiI SgrAI SnaBI StuI SwaI XhoI