Restriction map for
pF1KB3824
(Circular) (Six-base) MAPSORT of: pF1KB3824.seq Check: 7482 from: 1 to: 6221
pF1KB3824 6221 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1515 2041 2221 1515
Size: 526 180 5515
Fragments arranged by size:
5515 526 180
AccI GT'mk_AC
Cuts at: 3259 3259
Size: 6221
Acc65I G'GTAC_C
Cuts at: 3237 3237
Size: 6221
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1786 1883 2553 4046 4478 4831 5493 692
Size: 1094 97 670 1493 432 353 662 1420
Fragments arranged by size:
1493 1420 1094 670 662 432 353 97
AflIII A'CryG_T
Cuts at: 299 1148 3573 5373 5654 299
Size: 849 2425 1800 281 866
Fragments arranged by size:
2425 1800 866 849 281
AgeI A'CCGG_T
Cuts at: 1349 1349
Size: 6221
AhdI GACnn_n'nnGTC
Cuts at: 908 908
Size: 6221
AleI CACnn'nnGTG
Cuts at: 1781 2570 2900 1781
Size: 789 330 5102
Fragments arranged by size:
5102 789 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 972 972
Size: 6221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 2179
Size: 32 6189
AlwNI CAG_nnn'CTG
Cuts at: 257 734 3526 4964 257
Size: 477 2792 1438 1514
Fragments arranged by size:
2792 1514 1438 477
ApaLI G'TGCA_C
Cuts at: 2208 5059 2208
Size: 2851 3370
ApoI r'AATT_y
Cuts at: 3225 5574 5830 3225
Size: 2349 256 3616
Fragments arranged by size:
3616 2349 256
AseI AT'TA_AT
Cuts at: 20 1556 20
Size: 1536 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 203 261 3241 203
Size: 58 2980 3183
Fragments arranged by size:
3183 2980 58
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2406 2439 2406
Size: 33 6188
BamHI G'GATC_C
Cuts at: 1024 3246 1024
Size: 2222 3999
BanI G'GyrC_C
Cuts at: 149 199 334 1982 2040 3237 3651 3899 3934
Size: 50 135 1648 58 1197 414 248 35
Cuts at: 3934 149
Size: 2436
Fragments arranged by size:
2436 1648 1197 414 248 135 58 50
35
BanII G_rGCy'C
Cuts at: 251 1817 2126 2693 3235 4265 251
Size: 1566 309 567 542 1030 2207
Fragments arranged by size:
2207 1566 1030 567 542 309
BbeI G_GCGC'C
Cuts at: 3655 3903 3655
Size: 248 5973
BbsI GAAGACnn'nnnn_
Cuts at: 1367 2543 2776 1367
Size: 1176 233 4812
Fragments arranged by size:
4812 1176 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 679 713 679
Size: 34 6187
BciVI GTATCCnnnnn_n'
Cuts at: 1301 2493 2999 4119 5175 6221 1301
Size: 1192 506 1120 1056 1046 1301
Fragments arranged by size:
1301 1192 1120 1056 1046 506
BclI T'GATC_A
Cuts at: 2027 3741 2027
Size: 1714 4507
BglI GCCn_nnn'nGGC
Cuts at: 1190 2639 1190
Size: 1449 4772
BglII A'GATC_T
Cuts at: 1691 1829 3736 5773 1691
Size: 138 1907 2037 2139
Fragments arranged by size:
2139 2037 1907 138
BlpI GC'TnA_GC
Cuts at: 3333 3333
Size: 6221
Bme1580I G_kGCm'C
Cuts at: 152 1253 1927 2025 2212 2254 3846 3939 5063
Size: 1101 674 98 187 42 1592 93 1124
Cuts at: 5063 152
Size: 1310
Fragments arranged by size:
1592 1310 1124 1101 674 187 98 93
42
BmrI ACTGGGnnnn_n'
Cuts at: 1209 1264 3433 3514 3849 5993 1209
Size: 55 2169 81 335 2144 1437
Fragments arranged by size:
2169 2144 1437 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1005 4588 1005
Size: 3583 2638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2818 2851 2174
Size: 644 33 5544
Fragments arranged by size:
5544 644 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 2523 3397 4276 4990 5288 917
Size: 1606 874 879 714 298 1850
Fragments arranged by size:
1850 1606 879 874 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 165 1340 5925 165
Size: 1175 4585 461
Fragments arranged by size:
4585 1175 461
BsaAI yAC'GTr
Cuts at: 302 1248 3574 302
Size: 946 2326 2949
Fragments arranged by size:
2949 2326 946
BsaBI GATnn'nnATC
Cuts at: 6 1946 3735 5772 6
Size: 1940 1789 2037 455
Fragments arranged by size:
2037 1940 1789 455
BsaHI Gr'CG_yC
Cuts at: 281 3652 3900 4602 6098 281
Size: 3371 248 702 1496 404
Fragments arranged by size:
3371 1496 702 404 248
BsaWI w'CCGG_w
Cuts at: 1349 3410 3931 4609 5020 5167 5441 1349
Size: 2061 521 678 411 147 274 2129
Fragments arranged by size:
2129 2061 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2179 2209 2179
Size: 30 6191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 692 1978 2171 2608 2798 259
Size: 433 1286 193 437 190 3682
Fragments arranged by size:
3682 1286 437 433 193 190
BseYI C'CCAG_C
Cuts at: 168 727 1903 3646 4404 5069 5477 168
Size: 559 1176 1743 758 665 408 912
Fragments arranged by size:
1743 1176 912 758 665 559 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1353 1416 1515 1626 1743 1902 2114 2131
Size: 1058 63 99 111 117 159 212 17
Cuts at: 2131 2601 2878 295
Size: 470 277 3638
Fragments arranged by size:
3638 1058 470 277 212 159 117 111
99 63 17
BsiEI CG_ry'CG
Cuts at: 104 363 1415 2660 3763 3809 5039 104
Size: 259 1052 1245 1103 46 1230 1286
Fragments arranged by size:
1286 1245 1230 1103 1052 259 46
BsiHKAI G_wGCw'C
Cuts at: 1817 2126 2212 3235 4013 4203 5063 1817
Size: 309 86 1023 778 190 860 2975
Fragments arranged by size:
2975 1023 860 778 309 190 86
BsmI GAATG_Cn'
Cuts at: 889 1996 2974 3125 889
Size: 1107 978 151 3985
Fragments arranged by size:
3985 1107 978 151
Bsp1286I G_dGCh'C
Cuts at: 152 251 1253 1817 1927 2025 2126 2212 2254
Size: 99 1002 564 110 98 101 86 42
Cuts at: 2254 2693 3235 3846 3939 4013 4203 4265 5063
Size: 439 542 611 93 74 190 62 798
Cuts at: 5063 152
Size: 1310
Fragments arranged by size:
1310 1002 798 611 564 542 439 190
110 101 99 98 93 86 74 62
42
BspHI T'CATG_A
Cuts at: 4653 4653
Size: 6221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 556 1438 1515 2041 2221 3271 3485 3787
Size: 192 882 77 526 180 1050 214 302
Cuts at: 3787 4168 364
Size: 381 2417
Fragments arranged by size:
2417 1050 882 526 381 302 214 192
180 77
BsrBI CCG'CTC
Cuts at: 50 393 2598 4513 4567 6051 6216 50
Size: 343 2205 1915 54 1484 165 55
Fragments arranged by size:
2205 1915 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 489 2924 4133 489
Size: 2435 1209 2577
Fragments arranged by size:
2577 2435 1209
BsrFI r'CCGG_y
Cuts at: 139 1349 4219 4400 139
Size: 1210 2870 181 1960
Fragments arranged by size:
2870 1960 1210 181
BsrGI T'GTAC_A
Cuts at: 2308 2308
Size: 6221
BssHII G'CGCG_C
Cuts at: 190 190
Size: 6221
BssSI C'ACGA_G
Cuts at: 1046 4492 5200 1046
Size: 3446 708 2067
Fragments arranged by size:
3446 2067 708
BstAPI GCAn_nnn'nTGC
Cuts at: 770 1376 2624 3598 770
Size: 606 1248 974 3393
Fragments arranged by size:
3393 1248 974 606
BstBI TT'CG_AA
Cuts at: 543 1155 4582 543
Size: 612 3427 2182
Fragments arranged by size:
3427 2182 612
BstEII G'GTnAC_C
Cuts at: 2860 2860
Size: 6221
BstXI CCAn_nnnn'nTGG
Cuts at: 114 466 2152 2572 2700 114
Size: 352 1686 420 128 3635
Fragments arranged by size:
3635 1686 420 352 128
BstYI r'GATC_y
Cuts at: 1 1024 1281 1691 1829 3246 3713 3736 4071
Size: 1023 257 410 138 1417 467 23 335
Cuts at: 4071 4317 4623 4635 4721 5773 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1417 1052 1023 467 449 410 335 306
257 246 138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 810 810
Size: 6221
BtgI C'CryG_G
Cuts at: 1341 1937 1341
Size: 596 5625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1067 4171 4352 4515 1067
Size: 3104 181 163 2773
Fragments arranged by size:
3104 2773 181 163
BtsI GCAGTG_nn'
Cuts at: 2622 3010 3464 2622
Size: 388 454 5379
Fragments arranged by size:
5379 454 388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1742 1777 2537 2572 1742
Size: 35 760 35 5391
Fragments arranged by size:
5391 760 35 35
DraI TTT'AAA
Cuts at: 474 599 3220 474
Size: 125 2621 3475
Fragments arranged by size:
3475 2621 125
DrdI GACnn_nn'nnGTC
Cuts at: 3927 5271 3927
Size: 1344 4877
EaeI y'GGCC_r
Cuts at: 137 3806 3980 4371 4398 5743 137
Size: 3669 174 391 27 1345 615
Fragments arranged by size:
3669 1345 615 391 174 27
EagI C'GGCC_G
Cuts at: 3806 3806
Size: 6221
EarI CTCTTCn'nnn_
Cuts at: 608 4244 4454 608
Size: 3636 210 2375
Fragments arranged by size:
3636 2375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 4429 5171 5317 6031 373
Size: 4056 742 146 714 563
Fragments arranged by size:
4056 742 714 563 146
EcoICRI GAG'CTC
Cuts at: 1815 2124 3233 1815
Size: 309 1109 4803
Fragments arranged by size:
4803 1109 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1786 1883 2174 2553 2818 2851 4046 4478
Size: 1094 97 291 379 265 33 1195 432
Cuts at: 4478 4831 5493 692
Size: 353 662 1420
Fragments arranged by size:
1420 1195 1094 662 432 379 353 291
265 97 33
EcoO109I rG'GnC_Cy
Cuts at: 2604 3360 2604
Size: 756 5465
EcoRI G'AATT_C
Cuts at: 3225 3225
Size: 6221
EcoRV GAT'ATC
Cuts at: 2835 2835
Size: 6221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4240 4240
Size: 6221
FspI TGC'GCA
Cuts at: 763 4002 763
Size: 3239 2982
HaeII r_GCGC'y
Cuts at: 211 3655 3903 5133 5904 211
Size: 3444 248 1230 771 528
Fragments arranged by size:
3444 1230 771 528 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 894 926 1009 1041 1718 1750 2763 2795 4063
Size: 32 83 32 677 32 1013 32 1268
Cuts at: 4063 4093 4095 4125 894
Size: 30 2 30 2990
Fragments arranged by size:
2990 1268 1013 677 83 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1389 2793 3260 1389
Size: 1404 467 4350
Fragments arranged by size:
4350 1404 467
KasI G'GCGC_C
Cuts at: 3651 3899 3651
Size: 248 5973
KpnI G_GTAC'C
Cuts at: 3241 3241
Size: 6221
MluI A'CGCG_T
Cuts at: 5654 5654
Size: 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1567 4980 5164 1567
Size: 3413 184 2624
Fragments arranged by size:
3413 2624 184
MscI TGG'CCA
Cuts at: 3982 3982
Size: 6221
MslI CAynn'nnrTG
Cuts at: 796 1509 1781 1958 2570 2698 2900 4210 4337
Size: 713 272 177 612 128 202 1310 127
Cuts at: 4337 796
Size: 2680
Fragments arranged by size:
2680 1310 713 612 272 202 177 128
127
MspA1I CmG'CkG
Cuts at: 641 734 1309 1903 2222 2259 2398 2429 2615
Size: 93 575 594 319 37 139 31 186
Cuts at: 2615 2666 3332 3646 4006 4790 5035 641
Size: 51 666 314 360 784 245 1827
Fragments arranged by size:
1827 784 666 594 575 360 319 314
245 186 139 93 51 37 31
NaeI GCC'GGC
Cuts at: 141 4402 141
Size: 4261 1960
NarI GG'CG_CC
Cuts at: 3652 3900 3652
Size: 248 5973
NgoMIV G'CCGG_C
Cuts at: 139 4400 139
Size: 4261 1960
NspI r_CATG'y
Cuts at: 1152 1514 3171 3274 3774 5377 1152
Size: 362 1657 103 500 1603 1996
Fragments arranged by size:
1996 1657 1603 500 362 103
PciI A'CATG_T
Cuts at: 1148 5373 1148
Size: 4225 1996
PflMI CCAn_nnn'nTGG
Cuts at: 3213 3213
Size: 6221
PfoI T'CCnGG_A
Cuts at: 2404 2404
Size: 6221
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PmlI CAC'GTG
Cuts at: 302 302
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2211 4667 4699 2179
Size: 32 2456 32 3701
Fragments arranged by size:
3701 2456 32 32
PpuMI rG'GwC_Cy
Cuts at: 2604 2604
Size: 6221
PshAI GACnn'nnGTC
Cuts at: 1166 1790 1166
Size: 624 5597
PsiI TTA'TAA
Cuts at: 2968 2968
Size: 6221
PstI C_TGCA'G
Cuts at: 553 874 951 2639 3268 3953 553
Size: 321 77 1688 629 685 2821
Fragments arranged by size:
2821 1688 685 629 321 77
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 1903 2398 2429 2615 2666 3646 4006 1903
Size: 495 31 186 51 980 360 4118
Fragments arranged by size:
4118 980 495 360 186 51 31
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 1817 2126 3235 1817
Size: 309 1109 4803
Fragments arranged by size:
4803 1109 309
SalI G'TCGA_C
Cuts at: 3258 3258
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 4244 4454 4244
Size: 210 6011
SbfI CC_TGCA'GG
Cuts at: 2639 3268 2639
Size: 629 5592
ScaI AGT'ACT
Cuts at: 2130 5738 2130
Size: 3608 2613
SfcI C'TryA_G
Cuts at: 33 549 870 947 2635 3162 3187 3264 3949
Size: 516 321 77 1688 527 25 77 685
Cuts at: 3949 4917 5108 33
Size: 968 191 1146
Fragments arranged by size:
1688 1146 968 685 527 516 321 191
77 77 25
SfoI GGC'GCC
Cuts at: 3653 3901 3653
Size: 248 5973
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SmaI CCC'GGG
Cuts at: 205 263 3243 205
Size: 58 2980 3183
Fragments arranged by size:
3183 2980 58
SmlI C'TyrA_G
Cuts at: 932 2502 3376 4291 5005 5267 932
Size: 1570 874 915 714 262 1886
Fragments arranged by size:
1886 1570 915 874 714 262
SpeI A'CTAG_T
Cuts at: 67 795 67
Size: 728 5493
SphI G_CATG'C
Cuts at: 3171 3274 3774 3171
Size: 103 500 5618
Fragments arranged by size:
5618 500 103
SspI AAT'ATT
Cuts at: 1135 1135
Size: 6221
StuI AGG'CCT
Cuts at: 1891 2581 1891
Size: 690 5531
StyI C'CwwG_G
Cuts at: 632 1185 1708 1838 3355 3709 632
Size: 553 523 130 1517 354 3144
Fragments arranged by size:
3144 1517 553 523 354 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1926 1970 2299 2635 2912 3804 3937 4396 4605
Size: 44 329 336 277 892 133 459 209
Cuts at: 4605 1926
Size: 3542
Fragments arranged by size:
3542 892 459 336 329 277 209 133
44
TatI w'GTAC_w
Cuts at: 110 961 2128 2200 2308 5736 110
Size: 851 1167 72 108 3428 595
Fragments arranged by size:
3428 1167 851 595 108 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1485 1517 5738 5770 1485
Size: 32 4221 32 1936
Fragments arranged by size:
4221 1936 32 32
Tth111I GACn'n_nGTC
Cuts at: 4018 4018
Size: 6221
XbaI T'CTAG_A
Cuts at: 3252 3252
Size: 6221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 3017 3099 3716 114
Size: 2903 82 617 2619
Fragments arranged by size:
2903 2619 617 82
XmaI C'CCGG_G
Cuts at: 203 261 3241 203
Size: 58 2980 3183
Fragments arranged by size:
3183 2980 58
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BbvCI BfrBI
BmgBI BmtI Bpu10I BsiWI BsmBI BspEI BstZ17I ClaI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI