Restriction map for
pF1KB3818
(Circular) (Six-base) MAPSORT of: pF1KB3818.seq Check: 8974 from: 1 to: 4361
pF1KB3818 4361 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 494 494
Size: 4361
AccI GT'mk_AC
Cuts at: 542 1217 1399 542
Size: 675 182 3504
Fragments arranged by size:
3504 675 182
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2618 2971 3633 2186
Size: 432 353 662 2914
Fragments arranged by size:
2914 662 432 353
AflIII A'CryG_T
Cuts at: 117 1713 3513 3794 117
Size: 1596 1800 281 684
Fragments arranged by size:
1800 1596 684 281
AleI CACnn'nnGTG
Cuts at: 1253 1253
Size: 4361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1271 1271
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 1666 3104 1666
Size: 1438 2923
ApaLI G'TGCA_C
Cuts at: 3199 3199
Size: 4361
ApoI r'AATT_y
Cuts at: 403 847 988 1365 3714 3970 403
Size: 444 141 377 2349 256 794
Fragments arranged by size:
2349 794 444 377 256 141
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 373 1009 1082 1348 1381 373
Size: 636 73 266 33 3353
Fragments arranged by size:
3353 636 266 73 33
BamHI G'GATC_C
Cuts at: 299 1310 1386 299
Size: 1011 76 3274
Fragments arranged by size:
3274 1011 76
BanI G'GyrC_C
Cuts at: 184 1377 1791 2039 2074 184
Size: 1193 414 248 35 2471
Fragments arranged by size:
2471 1193 414 248 35
BanII G_rGCy'C
Cuts at: 736 755 1089 1148 1375 2405 736
Size: 19 334 59 227 1030 2692
Fragments arranged by size:
2692 1030 334 227 59 19
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 241 241
Size: 4361
BbvCI CC'TCA_GC
Cuts at: 326 326
Size: 4361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 664 698 664
Size: 34 4327
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3315 4361 2259
Size: 1056 1046 2259
Fragments arranged by size:
2259 1056 1046
BclI T'GATC_A
Cuts at: 443 1881 443
Size: 1438 2923
BglII A'GATC_T
Cuts at: 1876 3913 1876
Size: 2037 2324
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3203 1986
Size: 93 1124 3144
Fragments arranged by size:
3144 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1298 1573 1654 1989 4133 1298
Size: 275 81 335 2144 1526
Fragments arranged by size:
2144 1526 335 275 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 715 244
Size: 471 3890
Bpu10I CC'TnA_GC
Cuts at: 326 326
Size: 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1537 2416 3130 3428 397
Size: 1140 879 714 298 1330
Fragments arranged by size:
1330 1140 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1308 4065 1308
Size: 2757 1604
BsaAI yAC'GTr
Cuts at: 118 711 1714 118
Size: 593 1003 2765
Fragments arranged by size:
2765 1003 593
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 491 581 1792 2040 2742 4238 491
Size: 90 1211 248 702 1496 614
Fragments arranged by size:
1496 1211 702 614 248 90
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3160 3307 3581 1550
Size: 521 678 411 147 274 2330
Fragments arranged by size:
2330 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 718 748 1258 1288 718
Size: 30 510 30 3791
Fragments arranged by size:
3791 510 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1063 1263 1266 1063
Size: 200 3 4158
Fragments arranged by size:
4158 200 3
BseYI C'CCAG_C
Cuts at: 248 360 519 1314 1786 2544 3209 3617 248
Size: 112 159 795 472 758 665 408 992
Fragments arranged by size:
992 795 758 665 472 408 159 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1016 1231 238
Size: 778 215 3368
Fragments arranged by size:
3368 778 215
BsiEI CG_ry'CG
Cuts at: 104 1221 1903 1949 3179 104
Size: 1117 682 46 1230 1286
Fragments arranged by size:
1286 1230 1117 682 46
BsiHKAI G_wGCw'C
Cuts at: 716 1375 2153 2343 3203 716
Size: 659 778 190 860 1874
Fragments arranged by size:
1874 860 778 659 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1275 1275
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 716 736 755 1089 1148 1375 1986 2079 2153
Size: 20 19 334 59 227 611 93 74
Cuts at: 2153 2343 2405 3203 716
Size: 190 62 798 1874
Fragments arranged by size:
1874 798 611 334 227 190 93 74
62 59 20 19
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1411 1625 1927 2308 1411
Size: 214 302 381 3464
Fragments arranged by size:
3464 381 302 214
BsrBI CCG'CTC
Cuts at: 50 158 875 1029 2653 2707 4191 4356 50
Size: 108 717 154 1624 54 1484 165 55
Fragments arranged by size:
1624 1484 717 165 154 108 55 54
BsrDI GCAATG_nn'
Cuts at: 2273 2273
Size: 4361
BsrFI r'CCGG_y
Cuts at: 628 941 1124 1179 2359 2540 628
Size: 313 183 55 1180 181 2449
Fragments arranged by size:
2449 1180 313 183 181 55
BssSI C'ACGA_G
Cuts at: 2632 3340 2632
Size: 708 3653
BstAPI GCAn_nnn'nTGC
Cuts at: 1738 1738
Size: 4361
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 222 1183 222
Size: 961 3400
BstYI r'GATC_y
Cuts at: 1 299 1310 1386 1853 1876 2211 2457 2763
Size: 298 1011 76 467 23 335 246 306
Cuts at: 2763 2775 2861 3913 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1011 467 449 335 306 298 246
86 76 23 12
BtgI C'CryG_G
Cuts at: 107 1106 1148 107
Size: 999 42 3320
Fragments arranged by size:
3320 999 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 177 2311 2492 2655 177
Size: 2134 181 163 1883
Fragments arranged by size:
2134 1883 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1140 1604 1115
Size: 25 464 3872
Fragments arranged by size:
3872 464 25
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 3411 2067
Size: 1344 3017
EaeI y'GGCC_r
Cuts at: 431 563 1946 2120 2511 2538 3883 431
Size: 132 1383 174 391 27 1345 909
Fragments arranged by size:
1383 1345 909 391 174 132 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 928 940 2384 2594 928
Size: 12 1444 210 2695
Fragments arranged by size:
2695 1444 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 3311 3457 4171 2569
Size: 742 146 714 2759
Fragments arranged by size:
2759 742 714 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 715 2186 2618 2971 3633 244
Size: 471 1471 432 353 662 972
Fragments arranged by size:
1471 972 662 471 432 353
EcoO109I rG'GnC_Cy
Cuts at: 161 364 378 822 1500 161
Size: 203 14 444 678 3022
Fragments arranged by size:
3022 678 444 203 14
EcoRI G'AATT_C
Cuts at: 847 1365 847
Size: 518 3843
EcoRV GAT'ATC
Cuts at: 174 174
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 774 1795 2043 3273 4044 774
Size: 1021 248 1230 771 1091
Fragments arranged by size:
1230 1091 1021 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 2203 2233 2235 2265 134
Size: 32 2037 30 2 30 2230
Fragments arranged by size:
2230 2037 32 30 30 2
HincII GTy'rAC
Cuts at: 809 1218 1400 809
Size: 409 182 3770
Fragments arranged by size:
3770 409 182
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3120 3304 3120
Size: 184 4177
MscI TGG'CCA
Cuts at: 565 2122 565
Size: 1557 2804
MslI CAynn'nnrTG
Cuts at: 1253 2350 2477 1253
Size: 1097 127 3137
Fragments arranged by size:
3137 1097 127
MspA1I CmG'CkG
Cuts at: 252 1472 1786 2146 2930 3175 252
Size: 1220 314 360 784 245 1438
Fragments arranged by size:
1438 1220 784 360 314 245
NaeI GCC'GGC
Cuts at: 1181 2542 1181
Size: 1361 3000
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 107 107
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 1179 2540 1179
Size: 1361 3000
NspI r_CATG'y
Cuts at: 984 1414 1914 3517 984
Size: 430 500 1603 1828
Fragments arranged by size:
1828 1603 500 430
PciI A'CATG_T
Cuts at: 3513 3513
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 314 314
Size: 4361
PfoI T'CCnGG_A
Cuts at: 1306 1306
Size: 4361
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 253 285 367 399 2807 2839 253
Size: 32 82 32 2408 32 1775
Fragments arranged by size:
2408 1775 82 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 161 378 822 161
Size: 217 444 3700
Fragments arranged by size:
3700 444 217
PspXI vC'TCGA_Gb
Cuts at: 373 373
Size: 4361
PstI C_TGCA'G
Cuts at: 1408 2093 1408
Size: 685 3676
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 252 1786 2146 252
Size: 1534 360 2467
Fragments arranged by size:
2467 1534 360
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1216 1398 1216
Size: 182 4179
SapI GCTCTTCn'nnn_
Cuts at: 928 2384 2594 928
Size: 1456 210 2695
Fragments arranged by size:
2695 1456 210
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SexAI A'CCwGG_T
Cuts at: 536 536
Size: 4361
SfcI C'TryA_G
Cuts at: 33 1404 2089 3057 3248 33
Size: 1371 685 968 191 1146
Fragments arranged by size:
1371 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 373 412 1516 2431 3145 3407 373
Size: 39 1104 915 714 262 1327
Fragments arranged by size:
1327 1104 915 714 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StyI C'CwwG_G
Cuts at: 107 1495 1849 107
Size: 1388 354 2619
Fragments arranged by size:
2619 1388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 604 1119 1944 2077 2536 2745 172
Size: 432 515 825 133 459 209 1788
Fragments arranged by size:
1788 825 515 459 432 209 133
TatI w'GTAC_w
Cuts at: 719 3876 719
Size: 3157 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4329
Tth111I GACn'n_nGTC
Cuts at: 964 2158 964
Size: 1194 3167
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1856 1856
Size: 4361
XhoI C'TCGA_G
Cuts at: 373 373
Size: 4361
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
ZraI GAC'GTC
Cuts at: 492 492
Size: 4361
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI