Restriction map for
pF1KB3796
(Circular) (Six-base) MAPSORT of: pF1KB3796.seq Check: 64 from: 1 to: 5693
pF1KB3796 5693 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1615 1615
Size: 5693
AccI GT'mk_AC
Cuts at: 2731 2731
Size: 5693
Acc65I G'GTAC_C
Cuts at: 1115 1744 2709 1115
Size: 629 965 4099
Fragments arranged by size:
4099 965 629
AclI AA'CG_TT
Cuts at: 5533 5533
Size: 5693
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1354 1405 3518 3950 4303 4965 1354
Size: 51 2113 432 353 662 2082
Fragments arranged by size:
2113 2082 662 432 353 51
AflIII A'CryG_T
Cuts at: 468 508 1475 1817 3045 4845 5126 468
Size: 40 967 342 1228 1800 281 1035
Fragments arranged by size:
1800 1228 1035 967 342 281 40
AleI CACnn'nnGTG
Cuts at: 1937 1937
Size: 5693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1965 1965
Size: 5693
AlwNI CAG_nnn'CTG
Cuts at: 1058 2998 4436 1058
Size: 1940 1438 2315
Fragments arranged by size:
2315 1940 1438
ApaLI G'TGCA_C
Cuts at: 933 2441 4531 933
Size: 1508 2090 2095
Fragments arranged by size:
2095 2090 1508
ApoI r'AATT_y
Cuts at: 814 2096 2697 5046 5302 814
Size: 1282 601 2349 256 1205
Fragments arranged by size:
2349 1282 1205 601 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5693
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5693
AvaI C'yCGr_G
Cuts at: 191 2713 191
Size: 2522 3171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 407 440 407
Size: 33 5660
BamHI G'GATC_C
Cuts at: 266 2718 266
Size: 2452 3241
BanI G'GyrC_C
Cuts at: 1115 1723 1744 2007 2619 2709 3123 3371 3406
Size: 608 21 263 612 90 414 248 35
Cuts at: 3406 1115
Size: 3402
Fragments arranged by size:
3402 612 608 414 263 248 90 35
21
BanII G_rGCy'C
Cuts at: 2436 2612 2707 3737 2436
Size: 176 95 1030 4392
Fragments arranged by size:
4392 1030 176 95
BbeI G_GCGC'C
Cuts at: 3127 3375 3127
Size: 248 5445
BbsI GAAGACnn'nnnn_
Cuts at: 1379 2110 2161 2194 1379
Size: 731 51 33 4878
Fragments arranged by size:
4878 731 51 33
BbvCI CC'TCA_GC
Cuts at: 2390 2390
Size: 5693
BciVI GTATCCnnnnn_n'
Cuts at: 3591 4647 5693 3591
Size: 1056 1046 3591
Fragments arranged by size:
3591 1056 1046
BclI T'GATC_A
Cuts at: 344 3213 344
Size: 2869 2824
BglI GCCn_nnn'nGGC
Cuts at: 2005 2005
Size: 5693
BglII A'GATC_T
Cuts at: 3208 5245 3208
Size: 2037 3656
BlpI GC'TnA_GC
Cuts at: 2805 2805
Size: 5693
Bme1580I G_kGCm'C
Cuts at: 937 1496 2010 2445 2660 3318 3411 4535 937
Size: 559 514 435 215 658 93 1124 2095
Fragments arranged by size:
2095 1124 658 559 514 435 215 93
BmrI ACTGGGnnnn_n'
Cuts at: 265 2013 2391 2615 2905 2986 3321 5465 265
Size: 1748 378 224 290 81 335 2144 493
Fragments arranged by size:
2144 1748 493 378 335 290 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4060 4060
Size: 5693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 1192 1398 846
Size: 346 206 5141
Fragments arranged by size:
5141 346 206
Bpu10I CC'TnA_GC
Cuts at: 2390 2390
Size: 5693
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 3748 4462 4760 2869
Size: 879 714 298 3802
Fragments arranged by size:
3802 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 523 1156 5397 376
Size: 147 633 4241 672
Fragments arranged by size:
4241 672 633 147
BsaAI yAC'GTr
Cuts at: 509 1818 2440 3046 509
Size: 1309 622 606 3156
Fragments arranged by size:
3156 1309 622 606
BsaBI GATnn'nnATC
Cuts at: 6 3207 5244 6
Size: 3201 2037 455
Fragments arranged by size:
3201 2037 455
BsaHI Gr'CG_yC
Cuts at: 3124 3372 4074 5570 3124
Size: 248 702 1496 3247
Fragments arranged by size:
3247 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2882 3403 4081 4492 4639 4913 2882
Size: 521 678 411 147 274 3662
Fragments arranged by size:
3662 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1282 1282
Size: 5693
BseYI C'CCAG_C
Cuts at: 600 1014 1467 3118 3876 4541 4949 600
Size: 414 453 1651 758 665 408 1344
Fragments arranged by size:
1651 1344 758 665 453 414 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1425 1428 1468 1895 2465 1010
Size: 415 3 40 427 570 4238
Fragments arranged by size:
4238 570 427 415 40 3
BsiEI CG_ry'CG
Cuts at: 104 3235 3281 4511 104
Size: 3131 46 1230 1286
Fragments arranged by size:
3131 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 171 937 2445 2707 3485 3675 4535 171
Size: 766 1508 262 778 190 860 1329
Fragments arranged by size:
1508 1329 860 778 766 262 190
Bsp1286I G_dGCh'C
Cuts at: 171 937 1496 2010 2436 2445 2612 2660 2707
Size: 766 559 514 426 9 167 48 47
Cuts at: 2707 3318 3411 3485 3675 3737 4535 171
Size: 611 93 74 190 62 798 1329
Fragments arranged by size:
1329 798 766 611 559 514 426 190
167 93 74 62 48 47 9
BspHI T'CATG_A
Cuts at: 923 1038 1097 4125 923
Size: 115 59 3028 2491
Fragments arranged by size:
3028 2491 115 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1211 1490 1596 1615 2640 2743 2957 3259 3640
Size: 279 106 19 1025 103 214 302 381
Cuts at: 3640 1211
Size: 3264
Fragments arranged by size:
3264 1025 381 302 279 214 106 103
19
BsrBI CCG'CTC
Cuts at: 50 3985 4039 5523 5688 50
Size: 3935 54 1484 165 55
Fragments arranged by size:
3935 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 1320 1949 3605 374
Size: 946 629 1656 2462
Fragments arranged by size:
2462 1656 946 629
BsrFI r'CCGG_y
Cuts at: 163 1730 3691 3872 163
Size: 1567 1961 181 1984
Fragments arranged by size:
1984 1961 1567 181
BssHII G'CGCG_C
Cuts at: 115 115
Size: 5693
BssSI C'ACGA_G
Cuts at: 1546 1933 3964 4672 1546
Size: 387 2031 708 2567
Fragments arranged by size:
2567 2031 708 387
BstAPI GCAn_nnn'nTGC
Cuts at: 3070 3070
Size: 5693
BstBI TT'CG_AA
Cuts at: 4054 4054
Size: 5693
BstEII G'GTnAC_C
Cuts at: 2103 2103
Size: 5693
BstXI CCAn_nnnn'nTGG
Cuts at: 912 1240 2597 912
Size: 328 1357 4008
Fragments arranged by size:
4008 1357 328
BstYI r'GATC_y
Cuts at: 1 266 1598 2541 2662 2718 3185 3208 3543
Size: 265 1332 943 121 56 467 23 335
Cuts at: 3543 3789 4095 4107 4193 5245 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1332 1052 943 467 449 335 306 265
246 121 86 56 23 12
BtgI C'CryG_G
Cuts at: 107 462 1127 1953 2235 107
Size: 355 665 826 282 3565
Fragments arranged by size:
3565 826 665 355 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1844 3643 3824 3987 1844
Size: 1799 181 163 3550
Fragments arranged by size:
3550 1799 181 163
BtsI GCAGTG_nn'
Cuts at: 1461 1561 1954 1989 2347 2936 1461
Size: 100 393 35 358 589 4218
Fragments arranged by size:
4218 589 393 358 100 35
DraI TTT'AAA
Cuts at: 2692 2692
Size: 5693
DraIII CAC_nnn'GTG
Cuts at: 1871 2236 2655 1871
Size: 365 419 4909
Fragments arranged by size:
4909 419 365
DrdI GACnn_nn'nnGTC
Cuts at: 3399 4743 3399
Size: 1344 4349
EaeI y'GGCC_r
Cuts at: 161 1732 2227 2265 2549 2579 3278 3452 3843
Size: 1571 495 38 284 30 699 174 391
Cuts at: 3843 3870 5215 161
Size: 27 1345 639
Fragments arranged by size:
1571 1345 699 639 495 391 284 174
38 30 27
EagI C'GGCC_G
Cuts at: 3278 3278
Size: 5693
EarI CTCTTCn'nnn_
Cuts at: 213 271 601 1654 3716 3926 213
Size: 58 330 1053 2062 210 1980
Fragments arranged by size:
2062 1980 1053 330 210 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 3901 4643 4789 5503 2602
Size: 1299 742 146 714 2792
Fragments arranged by size:
2792 1299 742 714 146
EcoICRI GAG'CTC
Cuts at: 2705 2705
Size: 5693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 1192 1354 1398 1405 3518 3950 4303 4965
Size: 346 162 44 7 2113 432 353 662
Cuts at: 4965 846
Size: 1574
Fragments arranged by size:
2113 1574 662 432 353 346 162 44
7
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 2580
Size: 5693
EcoO109I rG'GnC_Cy
Cuts at: 258 792 1811 2501 2585 2832 258
Size: 534 1019 690 84 247 3119
Fragments arranged by size:
3119 1019 690 534 247 84
EcoRI G'AATT_C
Cuts at: 2096 2697 2096
Size: 601 5092
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1683 2049 3712 1683
Size: 366 1663 3664
Fragments arranged by size:
3664 1663 366
FspI TGC'GCA
Cuts at: 869 1353 3474 869
Size: 484 2121 3088
Fragments arranged by size:
3088 2121 484
FspAI rTGC'GCAy
Cuts at: 1353 1353
Size: 5693
HaeII r_GCGC'y
Cuts at: 3127 3375 4605 5376 3127
Size: 248 1230 771 3444
Fragments arranged by size:
3444 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 336 368 578 610 763 795 1079 1111 1480
Size: 32 210 32 153 32 284 32 369
Cuts at: 1480 1512 3535 3565 3567 3597 336
Size: 32 2023 30 2 30 2432
Fragments arranged by size:
2432 2023 369 284 210 153 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2355 2402 2732 2355
Size: 47 330 5316
Fragments arranged by size:
5316 330 47
HindIII A'AGCT_T
Cuts at: 838 838
Size: 5693
KasI G'GCGC_C
Cuts at: 3123 3371 3123
Size: 248 5445
KpnI G_GTAC'C
Cuts at: 1119 1748 2713 1119
Size: 629 965 4099
Fragments arranged by size:
4099 965 629
MluI A'CGCG_T
Cuts at: 5126 5126
Size: 5693
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2268 4452 4636 2268
Size: 2184 184 3325
Fragments arranged by size:
3325 2184 184
MscI TGG'CCA
Cuts at: 2551 2581 3454 2551
Size: 30 873 4790
Fragments arranged by size:
4790 873 30
MslI CAynn'nnrTG
Cuts at: 467 863 910 928 1937 3682 3809 467
Size: 396 47 18 1009 1745 127 2351
Fragments arranged by size:
2351 1745 1009 396 127 47 18
MspA1I CmG'CkG
Cuts at: 123 1383 2054 2804 3118 3478 4262 4507 123
Size: 1260 671 750 314 360 784 245 1309
Fragments arranged by size:
1309 1260 784 750 671 360 314 245
NaeI GCC'GGC
Cuts at: 1732 3874 1732
Size: 2142 3551
NarI GG'CG_CC
Cuts at: 3124 3372 3124
Size: 248 5445
NcoI C'CATG_G
Cuts at: 107 462 1127 107
Size: 355 665 4673
Fragments arranged by size:
4673 665 355
NgoMIV G'CCGG_C
Cuts at: 1730 3872 1730
Size: 2142 3551
NspI r_CATG'y
Cuts at: 472 933 1839 2746 3246 4849 472
Size: 461 906 907 500 1603 1316
Fragments arranged by size:
1603 1316 907 906 500 461
PasI CC'CwG_GG
Cuts at: 1594 1846 2496 1594
Size: 252 650 4791
Fragments arranged by size:
4791 650 252
PciI A'CATG_T
Cuts at: 468 4845 468
Size: 4377 1316
PflMI CCAn_nnn'nTGG
Cuts at: 1741 2236 1741
Size: 495 5198
PmeI GTTT'AAAC
Cuts at: 2692 2692
Size: 5693
PmlI CAC'GTG
Cuts at: 509 1818 2440 509
Size: 1309 622 3762
Fragments arranged by size:
3762 1309 622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 4139
Size: 32 5661
PpuMI rG'GwC_Cy
Cuts at: 258 792 1811 2501 2585 258
Size: 534 1019 690 84 3366
Fragments arranged by size:
3366 1019 690 534 84
PstI C_TGCA'G
Cuts at: 1395 1626 1784 2342 2541 2740 3425 1395
Size: 231 158 558 199 199 685 3663
Fragments arranged by size:
3663 685 558 231 199 199 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5693
PvuII CAG'CTG
Cuts at: 1383 3118 3478 1383
Size: 1735 360 3598
Fragments arranged by size:
3598 1735 360
RsrII CG'GwC_CG
Cuts at: 3888 3888
Size: 5693
SacI G_AGCT'C
Cuts at: 2707 2707
Size: 5693
SalI G'TCGA_C
Cuts at: 2730 2730
Size: 5693
SanDI GG'GwC_CC
Cuts at: 1811 1811
Size: 5693
SapI GCTCTTCn'nnn_
Cuts at: 213 271 3716 3926 213
Size: 58 3445 210 1980
Fragments arranged by size:
3445 1980 210 58
SbfI CC_TGCA'GG
Cuts at: 1395 2740 1395
Size: 1345 4348
ScaI AGT'ACT
Cuts at: 5210 5210
Size: 5693
SexAI A'CCwGG_T
Cuts at: 1575 1575
Size: 5693
SfcI C'TryA_G
Cuts at: 33 1059 1391 1622 1780 2338 2537 2736 3421
Size: 1026 332 231 158 558 199 199 685
Cuts at: 3421 4389 4580 33
Size: 968 191 1146
Fragments arranged by size:
1146 1026 968 685 558 332 231 199
199 191 158
SfoI GGC'GCC
Cuts at: 3125 3373 3125
Size: 248 5445
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5693
SmaI CCC'GGG
Cuts at: 2715 2715
Size: 5693
SmlI C'TyrA_G
Cuts at: 2848 3763 4477 4739 2848
Size: 915 714 262 3802
Fragments arranged by size:
3802 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5693
SphI G_CATG'C
Cuts at: 2746 3246 2746
Size: 500 5193
SspI AAT'ATT
Cuts at: 315 1914 315
Size: 1599 4094
StuI AGG'CCT
Cuts at: 2282 2282
Size: 5693
StyI C'CwwG_G
Cuts at: 107 213 462 894 984 1127 2079 2271 2576
Size: 106 249 432 90 143 952 192 305
Cuts at: 2576 2827 3181 107
Size: 251 354 2619
Fragments arranged by size:
2619 952 432 354 305 251 249 192
143 106 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2683 3276 3409 3868 4077 2683
Size: 593 133 459 209 4299
Fragments arranged by size:
4299 593 459 209 133
TatI w'GTAC_w
Cuts at: 1820 5208 1820
Size: 3388 2305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 518 550 1172 1204 1333 1365 2087 2119 2435
Size: 32 622 32 129 32 722 32 316
Cuts at: 2435 2467 5210 5242 518
Size: 32 2743 32 969
Fragments arranged by size:
2743 969 722 622 316 129 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3490 3490
Size: 5693
XbaI T'CTAG_A
Cuts at: 2724 2724
Size: 5693
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 909 2097 3188 909
Size: 1188 1091 3414
Fragments arranged by size:
3414 1188 1091
XmaI C'CCGG_G
Cuts at: 2713 2713
Size: 5693
XmnI GAAnn'nnTTC
Cuts at: 280 280
Size: 5693
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BspEI BsrGI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI