Restriction map for
pF1KB3778
(Circular) (Six-base) MAPSORT of: pF1KB3778.seq Check: 8836 from: 1 to: 3842
pF1KB3778 3842 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1667 2099 2846 3114 830
Size: 837 432 747 268 1558
Fragments arranged by size:
1558 837 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AhdI GACnn_n'nnGTC
Cuts at: 647 647
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 1147 2714 1147
Size: 1567 2275
ApaI G_GGCC'C
Cuts at: 202 202
Size: 3842
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 215 264 846 3195 3451 215
Size: 49 582 2349 256 606
Fragments arranged by size:
2349 606 582 256 49
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 230 784 862 230
Size: 554 78 3210
Fragments arranged by size:
3210 554 78
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 474 858 1272 1520 1555 474
Size: 384 414 248 35 2761
Fragments arranged by size:
2761 414 384 248 35
BanII G_rGCy'C
Cuts at: 202 856 1886 202
Size: 654 1030 2158
Fragments arranged by size:
2158 1030 654
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbsI GAAGACnn'nnnn_
Cuts at: 299 313 299
Size: 14 3828
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 202 1467 1560 2616 202
Size: 1265 93 1056 1428
Fragments arranged by size:
1428 1265 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 2209 442
Size: 1767 2075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 643 1273 1521 2223 3719 643
Size: 630 248 702 1496 766
Fragments arranged by size:
1496 766 702 630 248
BsaWI w'CCGG_w
Cuts at: 741 1031 1552 2230 2504 2651 3062 741
Size: 290 521 678 274 147 411 1521
Fragments arranged by size:
1521 678 521 411 290 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 702 732 702
Size: 30 3812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 478
Size: 3842
BseYI C'CCAG_C
Cuts at: 1267 2025 2602 3098 1267
Size: 758 577 496 2011
Fragments arranged by size:
2011 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 202 856 1467 1560 1634 1824 1886 2616 202
Size: 654 611 93 74 190 62 730 1428
Fragments arranged by size:
1428 730 654 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 553 2203 553
Size: 1650 2192
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 518 1792 1973 2136 518
Size: 1274 181 163 2224
Fragments arranged by size:
2224 1274 181 163
BtsI GCAGTG_nn'
Cuts at: 450 1085 450
Size: 635 3207
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 1427 1601 1992 2019 3364 1427
Size: 174 391 27 1345 1905
Fragments arranged by size:
1905 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 183 455 1865 2075 183
Size: 272 1410 210 1950
Fragments arranged by size:
1950 1410 272 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1667 2099 2846 3114 830
Size: 837 432 747 268 1558
Fragments arranged by size:
1558 837 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 199 981 199
Size: 782 3060
EcoRI G'AATT_C
Cuts at: 264 846 264
Size: 582 3260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 633 665 702 734 790 822 1684
Size: 32 288 32 37 32 56 32 862
Cuts at: 1684 1714 1716 1746 313
Size: 30 2 30 2409
Fragments arranged by size:
2409 862 288 56 37 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 368 1831 1958 368
Size: 1463 127 2252
Fragments arranged by size:
2252 1463 127
MspA1I CmG'CkG
Cuts at: 953 1267 1627 2640 2885 953
Size: 314 360 1013 245 1910
Fragments arranged by size:
1910 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PfoI T'CCnGG_A
Cuts at: 482 482
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PspOMI G'GGCC_C
Cuts at: 198 198
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 635 885 1570 2563 2754 33
Size: 602 250 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 250 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 976 1330 976
Size: 354 3488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
XmnI GAAnn'nnTTC
Cuts at: 192 264 192
Size: 72 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI