Restriction map for
pF1KB3766
(Circular) (Six-base) MAPSORT of: pF1KB3766.seq Check: 3832 from: 1 to: 4625
pF1KB3766 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 953 1118 1563 2450 2882 3235 3897 794
Size: 159 165 445 887 432 353 662 1522
Fragments arranged by size:
1522 887 662 445 432 353 165 159
AflIII A'CryG_T
Cuts at: 1977 3777 4058 1977
Size: 1800 281 2544
Fragments arranged by size:
2544 1800 281
AleI CACnn'nnGTG
Cuts at: 1365 1365
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 1550 1930 3368 1550
Size: 380 1438 2807
Fragments arranged by size:
2807 1438 380
ApaLI G'TGCA_C
Cuts at: 3463 3463
Size: 4625
ApoI r'AATT_y
Cuts at: 1606 1629 3978 4234 1606
Size: 23 2349 256 1997
Fragments arranged by size:
2349 1997 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 1645 1645
Size: 4625
AvrII C'CTAG_G
Cuts at: 718 718
Size: 4625
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 1370 1641 2055 2303 2338 1370
Size: 271 414 248 35 3657
Fragments arranged by size:
3657 414 271 248 35
BanII G_rGCy'C
Cuts at: 145 941 1639 2669 145
Size: 796 698 1030 2101
Fragments arranged by size:
2101 1030 796 698
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3579 4625 2523
Size: 1056 1046 2523
Fragments arranged by size:
2523 1056 1046
BclI T'GATC_A
Cuts at: 1224 2145 1224
Size: 921 3704
BglI GCCn_nnn'nGGC
Cuts at: 112 140 112
Size: 28 4597
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 2250 2343 3467 2250
Size: 93 1124 3408
Fragments arranged by size:
3408 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1837 1918 2253 4397 1837
Size: 81 335 2144 2065
Fragments arranged by size:
2144 2065 335 81
BmtI G_CTAG'C
Cuts at: 268 268
Size: 4625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
Bpu10I CC'TnA_GC
Cuts at: 1205 1584 1205
Size: 379 4246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3394 3692 1801
Size: 879 714 298 2734
Fragments arranged by size:
2734 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 809 4329 809
Size: 3520 1105
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 2056 2304 3006 4502 2056
Size: 248 702 1496 2179
Fragments arranged by size:
2179 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1814 2335 3013 3424 3571 3845 1814
Size: 521 678 411 147 274 2594
Fragments arranged by size:
2594 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 138 2050 2808 3473 3881 138
Size: 1912 758 665 408 882
Fragments arranged by size:
1912 882 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 4625
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3443 104
Size: 2063 46 1230 1286
Fragments arranged by size:
2063 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 503 941 1639 2417 2607 3467 503
Size: 438 698 778 190 860 1661
Fragments arranged by size:
1661 860 778 698 438 190
BsmI GAATG_Cn'
Cuts at: 1134 1134
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 145 503 941 1639 2250 2343 2417 2607 2669
Size: 358 438 698 611 93 74 190 62
Cuts at: 2669 3467 145
Size: 798 1303
Fragments arranged by size:
1303 798 698 611 438 358 190 93
74 62
BspHI T'CATG_A
Cuts at: 989 3057 989
Size: 2068 2557
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1675 1889 2191 2572 1675
Size: 214 302 381 3728
Fragments arranged by size:
3728 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 2537 1007
Size: 1530 3095
BsrFI r'CCGG_y
Cuts at: 134 2623 2804 134
Size: 2489 181 1955
Fragments arranged by size:
2489 1955 181
BssSI C'ACGA_G
Cuts at: 2896 3604 2896
Size: 708 3917
BstAPI GCAn_nnn'nTGC
Cuts at: 576 2002 576
Size: 1426 3199
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstEII G'GTnAC_C
Cuts at: 304 304
Size: 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 138 138
Size: 4625
BstYI r'GATC_y
Cuts at: 1 174 1494 1650 2117 2140 2475 2721 3027
Size: 173 1320 156 467 23 335 246 306
Cuts at: 3027 3039 3125 4177 1
Size: 12 86 1052 449
Fragments arranged by size:
1320 1052 467 449 335 306 246 173
156 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 419 1047 1360 1372 1868 419
Size: 628 313 12 496 3176
Fragments arranged by size:
3176 628 496 313 12
DraI TTT'AAA
Cuts at: 492 1605 1624 492
Size: 1113 19 3493
Fragments arranged by size:
3493 1113 19
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3675 2331
Size: 1344 3281
EaeI y'GGCC_r
Cuts at: 2210 2384 2775 2802 4147 2210
Size: 174 391 27 1345 2688
Fragments arranged by size:
2688 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 929 2648 2858 929
Size: 1719 210 2696
Fragments arranged by size:
2696 1719 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1604 2833 3575 3721 4435 1604
Size: 1229 742 146 714 1794
Fragments arranged by size:
1794 1229 742 714 146
EcoICRI GAG'CTC
Cuts at: 939 1637 939
Size: 698 3927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 953 1118 1563 2450 2882 3235 3897 794
Size: 159 165 445 887 432 353 662 1522
Fragments arranged by size:
1522 887 662 445 432 353 165 159
EcoO109I rG'GnC_Cy
Cuts at: 1764 1764
Size: 4625
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 394 432 484 2644 394
Size: 38 52 2160 2375
Fragments arranged by size:
2375 2160 52 38
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 2059 2307 3537 4308 2059
Size: 248 1230 771 2376
Fragments arranged by size:
2376 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 2467 2497 2499 2529 262
Size: 32 2173 30 2 30 2358
Fragments arranged by size:
2358 2173 32 30 30 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MfeI C'AATT_G
Cuts at: 403 403
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3384 3568 3384
Size: 184 4441
MscI TGG'CCA
Cuts at: 2386 2386
Size: 4625
MslI CAynn'nnrTG
Cuts at: 1365 1616 2614 2741 1365
Size: 251 998 127 3249
Fragments arranged by size:
3249 998 251 127
MspA1I CmG'CkG
Cuts at: 256 931 1438 1736 2050 2410 3194 3439 256
Size: 675 507 298 314 360 784 245 1442
Fragments arranged by size:
1442 784 675 507 360 314 298 245
NaeI GCC'GGC
Cuts at: 136 2806 136
Size: 2670 1955
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 107 107
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 134 2804 134
Size: 2670 1955
NheI G'CTAG_C
Cuts at: 264 264
Size: 4625
NspI r_CATG'y
Cuts at: 1678 2178 3781 1678
Size: 500 1603 2522
Fragments arranged by size:
2522 1603 500
PciI A'CATG_T
Cuts at: 3777 3777
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 815 847 1405 1437 3071 3103 815
Size: 32 558 32 1634 32 2337
Fragments arranged by size:
2337 1634 558 32 32 32
PsiI TTA'TAA
Cuts at: 553 703 785 1295 553
Size: 150 82 510 3883
Fragments arranged by size:
3883 510 150 82
PstI C_TGCA'G
Cuts at: 182 426 1367 1672 2357 182
Size: 244 941 305 685 2450
Fragments arranged by size:
2450 941 685 305 244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 256 931 1438 2050 2410 256
Size: 675 507 612 360 2471
Fragments arranged by size:
2471 675 612 507 360
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 941 1639 941
Size: 698 3927
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 929 2648 2858 929
Size: 1719 210 2696
Fragments arranged by size:
2696 1719 210
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 178 422 626 1363 1668 2353 3321 3512
Size: 145 244 204 737 305 685 968 191
Cuts at: 3512 33
Size: 1146
Fragments arranged by size:
1146 968 737 685 305 244 204 191
145
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 1780 2695 3409 3671 1780
Size: 915 714 262 2734
Fragments arranged by size:
2734 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StuI AGG'CCT
Cuts at: 1153 1153
Size: 4625
StyI C'CwwG_G
Cuts at: 107 718 1759 2113 107
Size: 611 1041 354 2619
Fragments arranged by size:
2619 1041 611 354
SwaI ATTT'AAAT
Cuts at: 1605 1605
Size: 4625
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1388 2208 2341 2800 3009 1388
Size: 820 133 459 209 3004
Fragments arranged by size:
3004 820 459 209 133
TatI w'GTAC_w
Cuts at: 800 4140 800
Size: 3340 1285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 811 843 4142 4174 811
Size: 32 3299 32 1262
Fragments arranged by size:
3299 1262 32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1245 1656 1245
Size: 411 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2120
Size: 4625
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
XmnI GAAnn'nnTTC
Cuts at: 1184 1420 1184
Size: 236 4389
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BmgBI
BpmI BsaXI BseRI BsiWI BsmBI BspEI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI XhoI ZraI