Restriction map for
pF1KB3747
(Circular) (Six-base) MAPSORT of: pF1KB3747.seq Check: 2226 from: 1 to: 4091
pF1KB3747 4091 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2348 3095 3363 1916
Size: 432 747 268 2644
Fragments arranged by size:
2644 747 432 268
AflIII A'CryG_T
Cuts at: 479 1443 2547 3524 479
Size: 964 1104 977 1046
Fragments arranged by size:
1104 1046 977 964
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AhdI GACnn_n'nnGTC
Cuts at: 576 576
Size: 4091
AleI CACnn'nnGTG
Cuts at: 922 922
Size: 4091
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 722 754 722
Size: 32 4059
AlwNI CAG_nnn'CTG
Cuts at: 1396 2963 1396
Size: 1567 2524
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 168 1095 3444 3700 168
Size: 927 2349 256 559
Fragments arranged by size:
2349 927 559 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 399 1111 399
Size: 712 3379
BamHI G'GATC_C
Cuts at: 881 1116 881
Size: 235 3856
BanI G'GyrC_C
Cuts at: 910 1107 1521 1769 1804 910
Size: 197 414 248 35 3197
Fragments arranged by size:
3197 414 248 197 35
BanII G_rGCy'C
Cuts at: 406 446 641 800 1105 2135 406
Size: 40 195 159 305 1030 2362
Fragments arranged by size:
2362 1030 305 195 159 40
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BbsI GAAGACnn'nnnn_
Cuts at: 131 146 482 131
Size: 15 336 3740
Fragments arranged by size:
3740 336 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 677 711 677
Size: 34 4057
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 413 1611 413
Size: 1198 2893
BglI GCCn_nnn'nGGC
Cuts at: 1006 1025 1006
Size: 19 4072
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 1716 1809 2865 1716
Size: 93 1056 2942
Fragments arranged by size:
2942 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 1267 2146 2638 2936 378
Size: 889 879 492 298 1533
Fragments arranged by size:
1533 889 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 312 1444 312
Size: 1132 2959
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 422 779 806 1522 1770 2472 3968 422
Size: 357 27 716 248 702 1496 545
Fragments arranged by size:
1496 716 702 545 357 248 27
BsaWI w'CCGG_w
Cuts at: 563 1280 1801 2479 2753 2900 3311 563
Size: 717 521 678 274 147 411 1343
Fragments arranged by size:
1343 717 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 722 752 814 844 722
Size: 30 62 30 3969
Fragments arranged by size:
3969 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 819 682
Size: 137 3954
BseYI C'CCAG_C
Cuts at: 1516 2274 2851 3347 1516
Size: 758 577 496 2260
Fragments arranged by size:
2260 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 580 1633 1679 2887 104
Size: 476 1053 46 1208 1308
Fragments arranged by size:
1308 1208 1053 476 46
BsiHKAI G_wGCw'C
Cuts at: 406 1105 1883 2073 2865 406
Size: 699 778 190 792 1632
Fragments arranged by size:
1632 792 778 699 190
Bsp1286I G_dGCh'C
Cuts at: 406 446 641 800 1105 1716 1809 1883 2073
Size: 40 195 159 305 611 93 74 190
Cuts at: 2073 2135 2865 406
Size: 62 730 1632
Fragments arranged by size:
1632 730 611 305 195 190 159 93
74 62 40
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1355 1657 2038 1141
Size: 214 302 381 3194
Fragments arranged by size:
3194 381 302 214
BsrBI CCG'CTC
Cuts at: 50 794 2383 2437 3921 4086 50
Size: 744 1589 54 1484 165 55
Fragments arranged by size:
1589 1484 744 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BsrGI T'GTAC_A
Cuts at: 431 431
Size: 4091
BssSI C'ACGA_G
Cuts at: 520 2362 2720 520
Size: 1842 358 1891
Fragments arranged by size:
1891 1842 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstYI r'GATC_y
Cuts at: 1 881 1116 1583 1606 1941 2187 3199 3285
Size: 880 235 467 23 335 246 1012 86
Cuts at: 3285 3297 3643 1
Size: 12 346 449
Fragments arranged by size:
1012 880 467 449 346 335 246 235
86 23 12
BtgI C'CryG_G
Cuts at: 448 773 448
Size: 325 3766
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1018 2041 2222 2385 1018
Size: 1023 181 163 2724
Fragments arranged by size:
2724 1023 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 134 1007 1676 1850 2241 2268 3613 134
Size: 873 669 174 391 27 1345 612
Fragments arranged by size:
1345 873 669 612 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 584 2114 2324 584
Size: 1530 210 2351
Fragments arranged by size:
2351 1530 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 513 2299 2609 2755 3901 513
Size: 1786 310 146 1146 703
Fragments arranged by size:
1786 1146 703 310 146
EcoICRI GAG'CTC
Cuts at: 404 1103 404
Size: 699 3392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1916 2348 3095 3363 349
Size: 1567 432 747 268 1077
Fragments arranged by size:
1567 1077 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 862 862
Size: 4091
EcoO109I rG'GnC_Cy
Cuts at: 516 733 1230 516
Size: 217 497 3377
Fragments arranged by size:
3377 497 217
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 1525 1773 2795 3774 1525
Size: 248 1022 979 1842
Fragments arranged by size:
1842 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 562 594 1933 1963 1965 1995 469
Size: 32 61 32 1339 30 2 30 2565
Fragments arranged by size:
2565 1339 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 180 810 1130 180
Size: 630 320 3141
Fragments arranged by size:
3141 630 320
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 560 2762 2946 560
Size: 2202 184 1705
Fragments arranged by size:
2202 1705 184
MscI TGG'CCA
Cuts at: 1009 1852 1009
Size: 843 3248
MslI CAynn'nnrTG
Cuts at: 382 646 922 2080 2207 382
Size: 264 276 1158 127 2266
Fragments arranged by size:
2266 1158 276 264 127
MspA1I CmG'CkG
Cuts at: 440 450 702 1202 1516 1876 2889 3134 440
Size: 10 252 500 314 360 1013 245 1397
Fragments arranged by size:
1397 1013 500 360 314 252 245 10
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NcoI C'CATG_G
Cuts at: 773 773
Size: 4091
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 483 1144 1644 2551 483
Size: 661 500 907 2023
Fragments arranged by size:
2023 907 661 500
PciI A'CATG_T
Cuts at: 479 2547 479
Size: 2068 2023
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 722 754 3230 3262 722
Size: 32 2476 32 1551
Fragments arranged by size:
2476 1551 32 32
PpuMI rG'GwC_Cy
Cuts at: 516 516
Size: 4091
PstI C_TGCA'G
Cuts at: 148 1138 1823 148
Size: 990 685 2416
Fragments arranged by size:
2416 990 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 702 1516 1876 702
Size: 814 360 2917
Fragments arranged by size:
2917 814 360
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 406 1105 406
Size: 699 3392
SacII CC_GC'GG
Cuts at: 451 451
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 144 533 1134 1819 2812 3003 33
Size: 111 389 601 685 993 191 1121
Fragments arranged by size:
1121 993 685 601 389 191 111
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 357 1246 2161 2653 2915 357
Size: 889 915 492 262 1533
Fragments arranged by size:
1533 915 889 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 773 1225 1579 773
Size: 452 354 3285
Fragments arranged by size:
3285 452 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 431 3606 431
Size: 3175 916
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI