Restriction map for
pF1KB3736
(Circular) (Six-base) MAPSORT of: pF1KB3736.seq Check: 1476 from: 1 to: 4466
pF1KB3736 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1059 1338 1059
Size: 279 4187
AccI GT'mk_AC
Cuts at: 788 1504 788
Size: 716 3750
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2291 2723 3076 3738 1246
Size: 1045 432 353 662 1974
Fragments arranged by size:
1974 1045 662 432 353
AfeI AGC'GCT
Cuts at: 526 526
Size: 4466
AflIII A'CryG_T
Cuts at: 1818 3618 3899 1818
Size: 1800 281 2385
Fragments arranged by size:
2385 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1274 1274
Size: 4466
AleI CACnn'nnGTG
Cuts at: 699 699
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 996 1771 3209 996
Size: 775 1438 2253
Fragments arranged by size:
2253 1438 775
ApaI G_GGCC'C
Cuts at: 921 921
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3304 3304
Size: 4466
ApoI r'AATT_y
Cuts at: 331 1470 3819 4075 331
Size: 1139 2349 256 722
Fragments arranged by size:
2349 1139 722 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 1486 1486
Size: 4466
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 1482 1896 2144 2179 1482
Size: 414 248 35 3769
Fragments arranged by size:
3769 414 248 35
BanII G_rGCy'C
Cuts at: 672 921 1163 1234 1480 2510 672
Size: 249 242 71 246 1030 2628
Fragments arranged by size:
2628 1030 249 246 242 71
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 330 336 330
Size: 6 4460
BbvCI CC'TCA_GC
Cuts at: 1210 1364 1210
Size: 154 4312
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3420 4466 2364
Size: 1056 1046 2364
Fragments arranged by size:
2364 1056 1046
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BglI GCCn_nnn'nGGC
Cuts at: 817 1229 817
Size: 412 4054
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1049 1162 1578 1049
Size: 113 416 3937
Fragments arranged by size:
3937 416 113
Bme1580I G_kGCm'C
Cuts at: 921 2091 2184 3308 921
Size: 1170 93 1124 2079
Fragments arranged by size:
2079 1170 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 123 668 1073 1678 1759 2094 4238 123
Size: 545 405 605 81 335 2144 351
Fragments arranged by size:
2144 605 545 405 351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 182 1389 2833 182
Size: 1207 1444 1815
Fragments arranged by size:
1815 1444 1207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 682 1181 1405 1474 390
Size: 292 499 224 69 3382
Fragments arranged by size:
3382 499 292 224 69
Bpu10I CC'TnA_GC
Cuts at: 912 1210 1364 912
Size: 298 154 4014
Fragments arranged by size:
4014 298 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3235 3533 1642
Size: 879 714 298 2575
Fragments arranged by size:
2575 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 657 1363 4170 657
Size: 706 2807 953
Fragments arranged by size:
2807 953 706
BsaAI yAC'GTr
Cuts at: 537 848 1819 537
Size: 311 971 3184
Fragments arranged by size:
3184 971 311
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 316 1897 2145 2847 4343 316
Size: 1581 248 702 1496 439
Fragments arranged by size:
1581 1496 702 439 248
BsaWI w'CCGG_w
Cuts at: 938 1655 2176 2854 3265 3412 3686 938
Size: 717 521 678 411 147 274 1718
Fragments arranged by size:
1718 717 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 696 726 696
Size: 30 4436
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 763 1216 1219 1255 1381 763
Size: 453 3 36 126 3848
Fragments arranged by size:
3848 453 126 36 3
BseYI C'CCAG_C
Cuts at: 573 1309 1891 2649 3314 3722 573
Size: 736 582 758 665 408 1317
Fragments arranged by size:
1317 758 736 665 582 408
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3284 104
Size: 1904 46 1230 1286
Fragments arranged by size:
1904 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 672 1050 1163 1480 2258 2448 3308 672
Size: 378 113 317 778 190 860 1830
Fragments arranged by size:
1830 860 778 378 317 190 113
Bsp1286I G_dGCh'C
Cuts at: 672 921 1050 1163 1234 1480 2091 2184 2258
Size: 249 129 113 71 246 611 93 74
Cuts at: 2258 2448 2510 3308 672
Size: 190 62 798 1830
Fragments arranged by size:
1830 798 611 249 246 190 129 113
93 74 71 62
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 1059 1338 1516 1730 2032 2413 374
Size: 685 279 178 214 302 381 2427
Fragments arranged by size:
2427 685 381 302 279 214 178
BsrBI CCG'CTC
Cuts at: 50 893 2758 2812 4296 4461 50
Size: 843 1865 54 1484 165 55
Fragments arranged by size:
1865 1484 843 165 55 54
BsrDI GCAATG_nn'
Cuts at: 291 2378 291
Size: 2087 2379
BsrFI r'CCGG_y
Cuts at: 1142 2464 2645 1142
Size: 1322 181 2963
Fragments arranged by size:
2963 1322 181
BssSI C'ACGA_G
Cuts at: 2737 3445 2737
Size: 708 3758
BstAPI GCAn_nnn'nTGC
Cuts at: 1843 1843
Size: 4466
BstBI TT'CG_AA
Cuts at: 1344 2827 1344
Size: 1483 2983
BstEII G'GTnAC_C
Cuts at: 903 1083 903
Size: 180 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 519 852 519
Size: 333 4133
BstYI r'GATC_y
Cuts at: 1 1148 1491 1958 1981 2316 2562 2868 2880
Size: 1147 343 467 23 335 246 306 12
Cuts at: 2880 2966 4018 1
Size: 86 1052 449
Fragments arranged by size:
1147 1052 467 449 343 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 623 107
Size: 516 3950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 971 1709 971
Size: 738 3728
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3516 2172
Size: 1344 3122
EaeI y'GGCC_r
Cuts at: 299 395 491 620 2051 2225 2616 2643 3988
Size: 96 96 129 1431 174 391 27 1345
Cuts at: 3988 299
Size: 777
Fragments arranged by size:
1431 1345 777 391 174 129 96 96
27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 150 250 2489 2699 150
Size: 100 2239 210 1917
Fragments arranged by size:
2239 1917 210 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 3416 3562 4276 2674
Size: 742 146 714 2864
Fragments arranged by size:
2864 742 714 146
EcoICRI GAG'CTC
Cuts at: 670 1161 1478 670
Size: 491 317 3658
Fragments arranged by size:
3658 491 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 682 1181 1246 1405 1474 2291 2723 3076
Size: 292 499 65 159 69 817 432 353
Cuts at: 3076 3738 390
Size: 662 1118
Fragments arranged by size:
1118 817 662 499 432 353 292 159
69 65
EcoO109I rG'GnC_Cy
Cuts at: 162 178 198 917 918 1605 162
Size: 16 20 719 1 687 3023
Fragments arranged by size:
3023 719 687 20 16 1
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4466
FspI TGC'GCA
Cuts at: 565 2247 565
Size: 1682 2784
FspAI rTGC'GCAy
Cuts at: 565 565
Size: 4466
HaeII r_GCGC'y
Cuts at: 528 1900 2148 3378 4149 528
Size: 1372 248 1230 771 845
Fragments arranged by size:
1372 1230 845 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1260 1292 2308 2338 2340 2370 1260
Size: 32 1016 30 2 30 3356
Fragments arranged by size:
3356 1016 32 30 30 2
HincII GTy'rAC
Cuts at: 1338 1505 1338
Size: 167 4299
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1139 3225 3409 1139
Size: 2086 184 2196
Fragments arranged by size:
2196 2086 184
MscI TGG'CCA
Cuts at: 397 493 622 2227 397
Size: 96 129 1605 2636
Fragments arranged by size:
2636 1605 129 96
MslI CAynn'nnrTG
Cuts at: 517 699 781 850 1031 1411 2455 2582 517
Size: 182 82 69 181 380 1044 127 2401
Fragments arranged by size:
2401 1044 380 182 181 127 82 69
MspA1I CmG'CkG
Cuts at: 292 840 1577 1891 2251 3035 3280 292
Size: 548 737 314 360 784 245 1478
Fragments arranged by size:
1478 784 737 548 360 314 245
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NcoI C'CATG_G
Cuts at: 107 623 107
Size: 516 3950
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NspI r_CATG'y
Cuts at: 801 1519 2019 3622 801
Size: 718 500 1603 1645
Fragments arranged by size:
1645 1603 718 500
PciI A'CATG_T
Cuts at: 3618 3618
Size: 4466
PfoI T'CCnGG_A
Cuts at: 200 1382 200
Size: 1182 3284
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PmlI CAC'GTG
Cuts at: 848 848
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 162 178 198 162
Size: 16 20 4430
Fragments arranged by size:
4430 20 16
PspOMI G'GGCC_C
Cuts at: 917 917
Size: 4466
PstI C_TGCA'G
Cuts at: 796 1256 1513 2198 796
Size: 460 257 685 3064
Fragments arranged by size:
3064 685 460 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 1891 2251 1891
Size: 360 4106
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 672 1163 1480 672
Size: 491 317 3658
Fragments arranged by size:
3658 491 317
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SanDI GG'GwC_CC
Cuts at: 162 198 162
Size: 36 4430
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 792 1252 1509 2194 3162 3353 33
Size: 759 460 257 685 968 191 1146
Fragments arranged by size:
1146 968 759 685 460 257 191
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 1488 1488
Size: 4466
SmlI C'TyrA_G
Cuts at: 1621 2536 3250 3512 1621
Size: 915 714 262 2575
Fragments arranged by size:
2575 915 714 262
SnaBI TAC'GTA
Cuts at: 537 537
Size: 4466
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StuI AGG'CCT
Cuts at: 616 616
Size: 4466
StyI C'CwwG_G
Cuts at: 107 617 623 1600 1954 107
Size: 510 6 977 354 2619
Fragments arranged by size:
2619 977 510 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 589 597 2049 2182 2641 2850 135
Size: 454 8 1452 133 459 209 1751
Fragments arranged by size:
1751 1452 459 454 209 133 8
TatI w'GTAC_w
Cuts at: 3981 3981
Size: 4466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 696 728 3983 4015 696
Size: 32 3255 32 1147
Fragments arranged by size:
3255 1147 32 32
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 433 1961 433
Size: 1528 2938
XmaI C'CCGG_G
Cuts at: 1486 1486
Size: 4466
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI