Restriction map for
pF1KB3726
(Circular) (Six-base) MAPSORT of: pF1KB3726.seq Check: 1361 from: 1 to: 5690
pF1KB3726 5690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2393 2393
Size: 5690
AatII G_ACGT'C
Cuts at: 2588 2588
Size: 5690
AccI GT'mk_AC
Cuts at: 2728 2728
Size: 5690
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 487 889 1126 1348 2026 3515 3947 4300
Size: 331 402 237 222 678 1489 432 353
Cuts at: 4300 4962 156
Size: 662 884
Fragments arranged by size:
1489 884 678 662 432 402 353 331
237 222
AfeI AGC'GCT
Cuts at: 2077 2077
Size: 5690
AflIII A'CryG_T
Cuts at: 788 3042 4842 5123 788
Size: 2254 1800 281 1355
Fragments arranged by size:
2254 1800 1355 281
AhdI GACnn_n'nnGTC
Cuts at: 1599 2574 1599
Size: 975 4715
AleI CACnn'nnGTG
Cuts at: 880 1939 2680 880
Size: 1059 741 3890
Fragments arranged by size:
3890 1059 741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 925 957 925
Size: 32 5658
AlwNI CAG_nnn'CTG
Cuts at: 636 1800 2356 2995 4433 636
Size: 1164 556 639 1438 1893
Fragments arranged by size:
1893 1438 1164 639 556
ApaI G_GGCC'C
Cuts at: 227 227
Size: 5690
ApaLI G'TGCA_C
Cuts at: 1213 4528 1213
Size: 3315 2375
ApoI r'AATT_y
Cuts at: 1969 1981 2694 5043 5299 1969
Size: 12 713 2349 256 2360
Fragments arranged by size:
2360 2349 713 256 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 396 1186 2293 2710 396
Size: 790 1107 417 3376
Fragments arranged by size:
3376 1107 790 417
BamHI G'GATC_C
Cuts at: 1391 1537 1805 2715 1391
Size: 146 268 910 4366
Fragments arranged by size:
4366 910 268 146
BanI G'GyrC_C
Cuts at: 2085 2706 3120 3368 3403 2085
Size: 621 414 248 35 4372
Fragments arranged by size:
4372 621 414 248 35
BanII G_rGCy'C
Cuts at: 227 238 1051 1513 1523 1754 1792 2468 2704
Size: 11 813 462 10 231 38 676 236
Cuts at: 2704 3734 227
Size: 1030 2183
Fragments arranged by size:
2183 1030 813 676 462 236 231 38
11 10
BbeI G_GCGC'C
Cuts at: 3124 3372 3124
Size: 248 5442
BbsI GAAGACnn'nnnn_
Cuts at: 1062 1117 2031 2190 1062
Size: 55 914 159 4562
Fragments arranged by size:
4562 914 159 55
BciVI GTATCCnnnnn_n'
Cuts at: 3588 4644 5690 3588
Size: 1056 1046 3588
Fragments arranged by size:
3588 1056 1046
BclI T'GATC_A
Cuts at: 3210 3210
Size: 5690
BglI GCCn_nnn'nGGC
Cuts at: 128 128
Size: 5690
BglII A'GATC_T
Cuts at: 419 737 1256 3205 5242 419
Size: 318 519 1949 2037 867
Fragments arranged by size:
2037 1949 867 519 318
BlpI GC'TnA_GC
Cuts at: 118 286 2802 118
Size: 168 2516 3006
Fragments arranged by size:
3006 2516 168
Bme1580I G_kGCm'C
Cuts at: 227 583 1217 2090 3315 3408 4532 227
Size: 356 634 873 1225 93 1124 1385
Fragments arranged by size:
1385 1225 1124 873 634 356 93
BmgBI CAC'GTC
Cuts at: 791 1068 1941 2682 791
Size: 277 873 741 3799
Fragments arranged by size:
3799 873 741 277
BmrI ACTGGGnnnn_n'
Cuts at: 1788 2902 2983 3318 5462 1788
Size: 1114 81 335 2144 2016
Fragments arranged by size:
2144 2016 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 712 1258 1477 1507 1589 1705 1804 394
Size: 318 546 219 30 82 116 99 4280
Fragments arranged by size:
4280 546 318 219 116 99 82 30
Bpu10I CC'TnA_GC
Cuts at: 1507 1552 1507
Size: 45 5645
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2173 2254 2635 2866 3745 4459 4757 1252
Size: 921 81 381 231 879 714 298 2185
Fragments arranged by size:
2185 921 879 714 381 298 231 81
BsaI GGTCTCn'nnnn_
Cuts at: 235 511 1912 2517 5394 235
Size: 276 1401 605 2877 531
Fragments arranged by size:
2877 1401 605 531 276
BsaAI yAC'GTr
Cuts at: 3043 3043
Size: 5690
BsaBI GATnn'nnATC
Cuts at: 6 1255 3204 5241 6
Size: 1249 1949 2037 455
Fragments arranged by size:
2037 1949 1249 455
BsaHI Gr'CG_yC
Cuts at: 317 2036 2585 3121 3369 4071 5567 317
Size: 1719 549 536 248 702 1496 440
Fragments arranged by size:
1719 1496 702 549 536 440 248
BsaWI w'CCGG_w
Cuts at: 2879 3400 4078 4489 4636 4910 2879
Size: 521 678 411 147 274 3659
Fragments arranged by size:
3659 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1741 1771 1741
Size: 30 5660
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 1780 2128 2152 2293 2599 226
Size: 1554 348 24 141 306 3317
Fragments arranged by size:
3317 1554 348 306 141 24
BseYI C'CCAG_C
Cuts at: 488 2322 3115 3873 4538 4946 488
Size: 1834 793 758 665 408 1232
Fragments arranged by size:
1834 1232 793 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 729
Size: 5690
BsiEI CG_ry'CG
Cuts at: 104 3232 3278 4508 104
Size: 3128 46 1230 1286
Fragments arranged by size:
3128 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 500 847 1051 1217 1513 1523 1532 2704 3482
Size: 347 204 166 296 10 9 1172 778
Cuts at: 3482 3672 4532 500
Size: 190 860 1658
Fragments arranged by size:
1658 1172 860 778 347 296 204 190
166 10 9
BsmBI CGTCTCn'nnnn_
Cuts at: 324 994 324
Size: 670 5020
Bsp1286I G_dGCh'C
Cuts at: 227 238 500 583 847 1051 1217 1513 1523
Size: 11 262 83 264 204 166 296 10
Cuts at: 1523 1532 1754 1792 2090 2468 2704 3315 3408
Size: 9 222 38 298 378 236 611 93
Cuts at: 3408 3482 3672 3734 4532 227
Size: 74 190 62 798 1385
Fragments arranged by size:
1385 798 611 378 298 296 264 262
236 222 204 190 166 93 83 74
62 38 11 10 9
BspHI T'CATG_A
Cuts at: 4122 4122
Size: 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 872 2393 2586 2627 2740 2954 3256 3637 872
Size: 1521 193 41 113 214 302 381 2925
Fragments arranged by size:
2925 1521 381 302 214 193 113 41
BsrBI CCG'CTC
Cuts at: 50 117 2301 2393 3982 4036 5520 5685 50
Size: 67 2184 92 1589 54 1484 165 55
Fragments arranged by size:
2184 1589 1484 165 92 67 55 54
BsrDI GCAATG_nn'
Cuts at: 3602 3602
Size: 5690
BsrFI r'CCGG_y
Cuts at: 2032 3688 3869 2032
Size: 1656 181 3853
Fragments arranged by size:
3853 1656 181
BsrGI T'GTAC_A
Cuts at: 656 1560 656
Size: 904 4786
BssSI C'ACGA_G
Cuts at: 3961 4669 3961
Size: 708 4982
BstAPI GCAn_nnn'nTGC
Cuts at: 3067 3067
Size: 5690
BstBI TT'CG_AA
Cuts at: 4051 4051
Size: 5690
BstEII G'GTnAC_C
Cuts at: 609 609
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 2682 2682
Size: 5690
BstYI r'GATC_y
Cuts at: 1 419 533 551 737 1256 1391 1488 1537
Size: 418 114 18 186 519 135 97 49
Cuts at: 1537 1805 1892 2715 3182 3205 3540 3786 4092
Size: 268 87 823 467 23 335 246 306
Cuts at: 4092 4104 4190 5242 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 823 519 467 449 418 335 306
268 246 186 135 114 97 87 86
49 23 18 12
Bsu36I CC'TnA_GG
Cuts at: 335 1801 335
Size: 1466 4224
BtgI C'CryG_G
Cuts at: 107 614 800 1913 2171 107
Size: 507 186 1113 258 3626
Fragments arranged by size:
3626 1113 507 258 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2241 2325 3640 3821 3984 2241
Size: 84 1315 181 163 3947
Fragments arranged by size:
3947 1315 181 163 84
BtsI GCAGTG_nn'
Cuts at: 986 2110 2933 986
Size: 1124 823 3743
Fragments arranged by size:
3743 1124 823
ClaI AT'CG_AT
Cuts at: 257 257
Size: 5690
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 607 3396 4740 607
Size: 2789 1344 1557
Fragments arranged by size:
2789 1557 1344
EaeI y'GGCC_r
Cuts at: 407 638 1163 2168 3275 3449 3840 3867 5212
Size: 231 525 1005 1107 174 391 27 1345
Cuts at: 5212 407
Size: 885
Fragments arranged by size:
1345 1107 1005 885 525 391 231 174
27
EagI C'GGCC_G
Cuts at: 3275 3275
Size: 5690
EarI CTCTTCn'nnn_
Cuts at: 410 631 2002 2119 2431 3713 3923 410
Size: 221 1371 117 312 1282 210 2177
Fragments arranged by size:
2177 1371 1282 312 221 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 4640 4786 5500 3898
Size: 742 146 714 4088
Fragments arranged by size:
4088 742 714 146
EcoICRI GAG'CTC
Cuts at: 1049 1511 1521 2702 1049
Size: 462 10 1181 4037
Fragments arranged by size:
4037 1181 462 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 394 487 712 889 1126 1258 1348 1477
Size: 238 93 225 177 237 132 90 129
Cuts at: 1477 1507 1589 1705 1804 2026 3515 3947 4300
Size: 30 82 116 99 222 1489 432 353
Cuts at: 4300 4962 156
Size: 662 884
Fragments arranged by size:
1489 884 662 432 353 238 237 225
222 177 132 129 116 99 93 90
82 30
EcoO109I rG'GnC_Cy
Cuts at: 129 223 224 763 2012 2604 2829 129
Size: 94 1 539 1249 592 225 2990
Fragments arranged by size:
2990 1249 592 539 225 94 1
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
EcoRV GAT'ATC
Cuts at: 2159 2159
Size: 5690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1041 2214 2295 3709 1041
Size: 1173 81 1414 3022
Fragments arranged by size:
3022 1414 1173 81
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 293 1326 2079 2188 3124 3372 4602 5373 293
Size: 1033 753 109 936 248 1230 771 610
Fragments arranged by size:
1230 1033 936 771 753 610 248 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 1383 1415 1585 1617 2560 2592 3532
Size: 32 1047 32 170 32 943 32 940
Cuts at: 3532 3562 3564 3594 304
Size: 30 2 30 2400
Fragments arranged by size:
2400 1047 943 940 170 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1845 2729 1845
Size: 884 4806
HindIII A'AGCT_T
Cuts at: 1549 1549
Size: 5690
KasI G'GCGC_C
Cuts at: 3120 3368 3120
Size: 248 5442
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 4449 4633 743
Size: 3706 184 1800
Fragments arranged by size:
3706 1800 184
MscI TGG'CCA
Cuts at: 409 640 2170 3451 409
Size: 231 1530 1281 2648
Fragments arranged by size:
2648 1530 1281 231
MslI CAynn'nnrTG
Cuts at: 880 1249 1939 2680 3679 3806 880
Size: 369 690 741 999 127 2764
Fragments arranged by size:
2764 999 741 690 369 127
MspA1I CmG'CkG
Cuts at: 435 700 903 1011 1747 1899 2801 3115 3475
Size: 265 203 108 736 152 902 314 360
Cuts at: 3475 4259 4504 435
Size: 784 245 1621
Fragments arranged by size:
1621 902 784 736 360 314 265 245
203 152 108
NaeI GCC'GGC
Cuts at: 3871 3871
Size: 5690
NarI GG'CG_CC
Cuts at: 3121 3369 3121
Size: 248 5442
NcoI C'CATG_G
Cuts at: 107 614 800 107
Size: 507 186 4997
Fragments arranged by size:
4997 507 186
NgoMIV G'CCGG_C
Cuts at: 3869 3869
Size: 5690
NspI r_CATG'y
Cuts at: 2743 3243 4846 2743
Size: 500 1603 3587
Fragments arranged by size:
3587 1603 500
PciI A'CATG_T
Cuts at: 4842 4842
Size: 5690
PflMI CCAn_nnn'nTGG
Cuts at: 636 1236 1928 636
Size: 600 692 4398
Fragments arranged by size:
4398 692 600
PfoI T'CCnGG_A
Cuts at: 145 536 1051 1605 1736 2282 145
Size: 391 515 554 131 546 3553
Fragments arranged by size:
3553 554 546 515 391 131
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1336 1368 4136 4168 1336
Size: 32 2768 32 2858
Fragments arranged by size:
2858 2768 32 32
PpuMI rG'GwC_Cy
Cuts at: 763 2012 2604 763
Size: 1249 592 3849
Fragments arranged by size:
3849 1249 592
PshAI GACnn'nnGTC
Cuts at: 1840 2035 1840
Size: 195 5495
PspOMI G'GGCC_C
Cuts at: 223 223
Size: 5690
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2650 2682 2650
Size: 32 5658
PstI C_TGCA'G
Cuts at: 911 981 1122 2624 2737 3422 911
Size: 70 141 1502 113 685 3179
Fragments arranged by size:
3179 1502 685 141 113 70
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 435 700 1011 1899 3115 3475 435
Size: 265 311 888 1216 360 2650
Fragments arranged by size:
2650 1216 888 360 311 265
RsrII CG'GwC_CG
Cuts at: 2310 3885 2310
Size: 1575 4115
SacI G_AGCT'C
Cuts at: 1051 1513 1523 2704 1051
Size: 462 10 1181 4037
Fragments arranged by size:
4037 1181 462 10
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 1169 1169
Size: 5690
SfcI C'TryA_G
Cuts at: 33 162 907 977 1118 1477 2151 2620 2733
Size: 129 745 70 141 359 674 469 113
Cuts at: 2733 3418 4386 4577 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 745 685 674 469 359 191
141 129 113 70
SfoI GGC'GCC
Cuts at: 3122 3370 3122
Size: 248 5442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 1267 2188 2269 2650 2845 3760 4474 4736 1267
Size: 921 81 381 195 915 714 262 2221
Fragments arranged by size:
2221 921 915 714 381 262 195 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 2743 3243 2743
Size: 500 5190
StuI AGG'CCT
Cuts at: 371 1498 371
Size: 1127 4563
StyI C'CwwG_G
Cuts at: 107 584 614 767 776 800 992 1646 1838
Size: 477 30 153 9 24 192 654 192
Cuts at: 1838 2081 2363 2824 3178 107
Size: 243 282 461 354 2619
Fragments arranged by size:
2619 654 477 461 354 282 243 192
192 153 30 24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2327 3273 3406 3865 4074 2327
Size: 946 133 459 209 3943
Fragments arranged by size:
3943 946 459 209 133
TatI w'GTAC_w
Cuts at: 656 1560 1673 2276 2666 5205 656
Size: 904 113 603 390 2539 1141
Fragments arranged by size:
2539 1141 904 603 390 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1741 1773 5207 5239 1741
Size: 32 3434 32 2192
Fragments arranged by size:
3434 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 832 2016 3487 832
Size: 1184 1471 3035
Fragments arranged by size:
3035 1471 1184
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1402 3185 1402
Size: 1783 3907
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
XmnI GAAnn'nnTTC
Cuts at: 1351 1466 1351
Size: 115 5575
ZraI GAC'GTC
Cuts at: 2586 2586
Size: 5690
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AlfI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsiWI BsmI BspEI BssHII BstZ17I CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI