Restriction map for
pF1KB3668
(Circular) (Six-base) MAPSORT of: pF1KB3668.seq Check: 9471 from: 1 to: 3896
pF1KB3668 3896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1721 2153 2900 3168 1721
Size: 432 747 268 2449
Fragments arranged by size:
2449 747 432 268
AflIII A'CryG_T
Cuts at: 619 1248 2352 3329 619
Size: 629 1104 977 1186
Fragments arranged by size:
1186 1104 977 629
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 1201 2768 1201
Size: 1567 2329
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 308 425 872 900 3249 3505 308
Size: 117 447 28 2349 256 699
Fragments arranged by size:
2349 699 447 256 117 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 122 916 122
Size: 794 3102
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 133 198 912 1326 1574 1609 133
Size: 65 714 414 248 35 2420
Fragments arranged by size:
2420 714 414 248 65 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 137 1330 1578 137
Size: 1193 248 2455
Fragments arranged by size:
2455 1193 248
BbsI GAAGACnn'nnnn_
Cuts at: 510 510
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3896
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 205 2263 205
Size: 2058 1838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 881
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1072 1951 2443 2741 839
Size: 233 879 492 298 1994
Fragments arranged by size:
1994 879 492 298 233
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 134 1327 1575 2277 3773 134
Size: 1193 248 702 1496 257
Fragments arranged by size:
1496 1193 702 257 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1321 2079 2656 3152 1321
Size: 758 577 496 2065
Fragments arranged by size:
2065 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 910 1688 1878 2670 173
Size: 737 778 190 792 1399
Fragments arranged by size:
1399 792 778 737 190
Bsp1286I G_dGCh'C
Cuts at: 173 910 1521 1614 1688 1878 1940 2670 173
Size: 737 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 737 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 237 3072 237
Size: 2835 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 780 1808 780
Size: 1028 2868
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 295 1139 295
Size: 844 3052
DraI TTT'AAA
Cuts at: 273 895 273
Size: 622 3274
DrdI GACnn_nn'nnGTC
Cuts at: 539 1602 2460 539
Size: 1063 858 1975
Fragments arranged by size:
1975 1063 858
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 1721 2153 2900 3168 881
Size: 840 432 747 268 1609
Fragments arranged by size:
1609 840 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1035 1035
Size: 3896
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 137 1330 1578 2600 3579 137
Size: 1193 248 1022 979 454
Fragments arranged by size:
1193 1022 979 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1738 1768 1770 1800 1738
Size: 30 2 30 3834
Fragments arranged by size:
3834 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
HindIII A'AGCT_T
Cuts at: 214 214
Size: 3896
KasI G'GCGC_C
Cuts at: 133 1326 1574 133
Size: 1193 248 2455
Fragments arranged by size:
2455 1193 248
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 757 1885 2012 757
Size: 1128 127 2641
Fragments arranged by size:
2641 1128 127
MspA1I CmG'CkG
Cuts at: 140 159 705 1007 1321 1681 2694 2939 140
Size: 19 546 302 314 360 1013 245 1097
Fragments arranged by size:
1097 1013 546 360 314 302 245 19
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 134 1327 1575 134
Size: 1193 248 2455
Fragments arranged by size:
2455 1193 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 319 949 1449 2356 319
Size: 630 500 907 1859
Fragments arranged by size:
1859 907 630 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PflMI CCAn_nnn'nTGG
Cuts at: 695 695
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 705 1321 1681 705
Size: 616 360 2920
Fragments arranged by size:
2920 616 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SexAI A'CCwGG_T
Cuts at: 688 688
Size: 3896
SfcI C'TryA_G
Cuts at: 33 648 939 1624 2617 2808 33
Size: 615 291 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 291 191
SfoI GGC'GCC
Cuts at: 135 1328 1576 135
Size: 1193 248 2455
Fragments arranged by size:
2455 1193 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 124 918 124
Size: 794 3102
SmlI C'TyrA_G
Cuts at: 854 1051 1966 2458 2720 854
Size: 197 915 492 262 2030
Fragments arranged by size:
2030 915 492 262 197
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
SspI AAT'ATT
Cuts at: 361 361
Size: 3896
StyI C'CwwG_G
Cuts at: 107 1030 1384 107
Size: 923 354 2619
Fragments arranged by size:
2619 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 3413 3445 274
Size: 32 3107 32 725
Fragments arranged by size:
3107 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 122 916 122
Size: 794 3102
XmnI GAAnn'nnTTC
Cuts at: 308 308
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI