Restriction map for
pF1KB3587
(Circular) (Six-base) MAPSORT of: pF1KB3587.seq Check: 6866 from: 1 to: 4577
pF1KB3587 4577 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 622 1286 622
Size: 664 3913
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 542 1593 542
Size: 1051 3526
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 2402 2834 3581 3849 432
Size: 1970 432 747 268 1160
Fragments arranged by size:
1970 1160 747 432 268
AflII C'TTAA_G
Cuts at: 841 841
Size: 4577
AflIII A'CryG_T
Cuts at: 1172 1929 3033 4010 1172
Size: 757 1104 977 1739
Fragments arranged by size:
1739 1104 977 757
AgeI A'CCGG_T
Cuts at: 2965 2965
Size: 4577
AlwNI CAG_nnn'CTG
Cuts at: 400 1422 1882 3449 400
Size: 1022 460 1567 1528
Fragments arranged by size:
1567 1528 1022 460
ApaI G_GGCC'C
Cuts at: 131 1061 1330 131
Size: 930 269 3378
Fragments arranged by size:
3378 930 269
ApaLI G'TGCA_C
Cuts at: 3347 3347
Size: 4577
ApoI r'AATT_y
Cuts at: 1207 1581 3930 4186 1207
Size: 374 2349 256 1598
Fragments arranged by size:
2349 1598 374 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 237 1282 1597 237
Size: 1045 315 3217
Fragments arranged by size:
3217 1045 315
BamHI G'GATC_C
Cuts at: 970 1602 970
Size: 632 3945
BanI G'GyrC_C
Cuts at: 526 542 1437 1593 2007 2255 2290 526
Size: 16 895 156 414 248 35 2813
Fragments arranged by size:
2813 895 414 248 156 35 16
BanII G_rGCy'C
Cuts at: 131 1061 1253 1330 1591 2621 131
Size: 930 192 77 261 1030 2087
Fragments arranged by size:
2087 1030 930 261 192 77
BbeI G_GCGC'C
Cuts at: 1441 2011 2259 1441
Size: 570 248 3759
Fragments arranged by size:
3759 570 248
BbsI GAAGACnn'nnnn_
Cuts at: 288 288
Size: 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1191 1225 90
Size: 34 1067 34 3442
Fragments arranged by size:
3442 1067 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 681 932 2097 681
Size: 251 1165 3161
Fragments arranged by size:
3161 1165 251
BglI GCCn_nnn'nGGC
Cuts at: 1452 1452
Size: 4577
BglII A'GATC_T
Cuts at: 2092 4129 2092
Size: 2037 2540
BlpI GC'TnA_GC
Cuts at: 1561 1689 1561
Size: 128 4449
Bme1580I G_kGCm'C
Cuts at: 131 1061 1303 1330 2202 2295 3351 131
Size: 930 242 27 872 93 1056 1357
Fragments arranged by size:
1357 1056 930 872 242 93 27
BmgBI CAC'GTC
Cuts at: 801 1383 801
Size: 582 3995
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1789 1870 2205 4349 1333
Size: 456 81 335 2144 1561
Fragments arranged by size:
2144 1561 456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1458 2944 1458
Size: 1486 3091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 886
Size: 4577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1753 2632 3124 3422 1753
Size: 879 492 298 2908
Fragments arranged by size:
2908 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1500 1930 1500
Size: 430 4147
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 293 1426 1438 2008 2256 2958 4454 293
Size: 1133 12 570 248 702 1496 416
Fragments arranged by size:
1496 1133 702 570 416 248 12
BsaWI w'CCGG_w
Cuts at: 1766 2287 2965 3239 3386 3797 1766
Size: 521 678 274 147 411 2546
Fragments arranged by size:
2546 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 157 187 157
Size: 30 4547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 162
Size: 4577
BseYI C'CCAG_C
Cuts at: 1252 1503 2002 2760 3337 3833 1252
Size: 251 499 758 577 496 1996
Fragments arranged by size:
1996 758 577 499 496 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 1258
Size: 4577
BsiEI CG_ry'CG
Cuts at: 104 2119 2165 3373 104
Size: 2015 46 1208 1308
Fragments arranged by size:
2015 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1406 1591 2369 2559 3351 1204
Size: 202 185 778 190 792 2430
Fragments arranged by size:
2430 792 778 202 190 185
BsmI GAATG_Cn'
Cuts at: 784 1193 784
Size: 409 4168
Bsp1286I G_dGCh'C
Cuts at: 131 1061 1204 1253 1303 1330 1406 1591 2202
Size: 930 143 49 50 27 76 185 611
Cuts at: 2202 2295 2369 2559 2621 3351 131
Size: 93 74 190 62 730 1357
Fragments arranged by size:
1357 930 730 611 190 185 143 93
76 74 62 50 49 27
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 272 3753 272
Size: 3481 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 1286 1627 1841 2143 2524 622
Size: 664 341 214 302 381 2675
Fragments arranged by size:
2675 664 381 341 302 214
BsrBI CCG'CTC
Cuts at: 50 2869 2923 4407 4572 50
Size: 2819 54 1484 165 55
Fragments arranged by size:
2819 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 645 2489 645
Size: 1844 2733
BsrFI r'CCGG_y
Cuts at: 2575 2756 2965 2575
Size: 181 209 4187
Fragments arranged by size:
4187 209 181
BssSI C'ACGA_G
Cuts at: 470 2848 3206 470
Size: 2378 358 1841
Fragments arranged by size:
2378 1841 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1307 1954 1307
Size: 647 3930
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 1458 1458
Size: 4577
BstYI r'GATC_y
Cuts at: 1 970 1602 2069 2092 2427 2673 3685 3771
Size: 969 632 467 23 335 246 1012 86
Cuts at: 3771 3783 4129 1
Size: 12 346 449
Fragments arranged by size:
1012 969 632 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 179 1568 179
Size: 1389 3188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2527 2708 2871 2527
Size: 181 163 4233
Fragments arranged by size:
4233 181 163
BtsI GCAGTG_nn'
Cuts at: 1820 1820
Size: 4577
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DraIII CAC_nnn'GTG
Cuts at: 1168 1168
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 460 2283 3141 460
Size: 1823 858 1896
Fragments arranged by size:
1896 1823 858
EaeI y'GGCC_r
Cuts at: 582 1511 2162 2336 2727 2754 4099 582
Size: 929 651 174 391 27 1345 1060
Fragments arranged by size:
1345 1060 929 651 391 174 27
EagI C'GGCC_G
Cuts at: 2162 2162
Size: 4577
EarI CTCTTCn'nnn_
Cuts at: 343 1080 2600 2810 343
Size: 737 1520 210 2110
Fragments arranged by size:
2110 1520 737 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2785 3095 3241 4387 2785
Size: 310 146 1146 2975
Fragments arranged by size:
2975 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 886 2402 2834 3581 3849 432
Size: 454 1516 432 747 268 1160
Fragments arranged by size:
1516 1160 747 454 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1363 1531 1363
Size: 168 4409
EcoO109I rG'GnC_Cy
Cuts at: 428 1716 428
Size: 1288 3289
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
EcoRV GAT'ATC
Cuts at: 516 516
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4577
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 1441 2011 2259 3281 4260 1441
Size: 570 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 570 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1079 1111 2419 2449 2451 2481 1079
Size: 32 1308 30 2 30 3175
Fragments arranged by size:
3175 1308 32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 1437 2007 2255 1437
Size: 570 248 3759
Fragments arranged by size:
3759 570 248
KpnI G_GTAC'C
Cuts at: 546 1597 546
Size: 1051 3526
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 965 3248 3432 965
Size: 2283 184 2110
Fragments arranged by size:
2283 2110 184
MscI TGG'CCA
Cuts at: 584 1513 2338 584
Size: 929 825 2823
Fragments arranged by size:
2823 929 825
MslI CAynn'nnrTG
Cuts at: 1141 1498 2566 2693 1141
Size: 357 1068 127 3025
Fragments arranged by size:
3025 1068 357 127
MspA1I CmG'CkG
Cuts at: 1688 2002 2362 3375 3620 1688
Size: 314 360 1013 245 2645
Fragments arranged by size:
2645 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 4577
NarI GG'CG_CC
Cuts at: 1438 2008 2256 1438
Size: 570 248 3759
Fragments arranged by size:
3759 570 248
NcoI C'CATG_G
Cuts at: 179 179
Size: 4577
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 4577
NspI r_CATG'y
Cuts at: 1176 1630 2130 3037 1176
Size: 454 500 907 2716
Fragments arranged by size:
2716 907 500 454
PasI CC'CwG_GG
Cuts at: 695 695
Size: 4577
PciI A'CATG_T
Cuts at: 1172 3033 1172
Size: 1861 2716
PfoI T'CCnGG_A
Cuts at: 863 863
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PmlI CAC'GTG
Cuts at: 1500 1500
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 3716
Size: 32 4545
PpuMI rG'GwC_Cy
Cuts at: 428 428
Size: 4577
PshAI GACnn'nnGTC
Cuts at: 292 310 292
Size: 18 4559
PspOMI G'GGCC_C
Cuts at: 127 1057 1326 127
Size: 930 269 3378
Fragments arranged by size:
3378 930 269
PstI C_TGCA'G
Cuts at: 396 1227 1244 1624 2309 396
Size: 831 17 380 685 2664
Fragments arranged by size:
2664 831 685 380 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 2002 2362 2002
Size: 360 4217
RsrII CG'GwC_CG
Cuts at: 2772 2772
Size: 4577
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SanDI GG'GwC_CC
Cuts at: 428 428
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 392 726 1011 1223 1240 1620 2305 3298
Size: 359 334 285 212 17 380 685 993
Cuts at: 3298 3489 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 380 359 334 285 212
191 17
SfoI GGC'GCC
Cuts at: 1439 2009 2257 1439
Size: 570 248 3759
Fragments arranged by size:
3759 570 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SmaI CCC'GGG
Cuts at: 239 1284 1599 239
Size: 1045 315 3217
Fragments arranged by size:
3217 1045 315
SmlI C'TyrA_G
Cuts at: 841 1732 2647 3139 3401 841
Size: 891 915 492 262 2017
Fragments arranged by size:
2017 915 891 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StyI C'CwwG_G
Cuts at: 179 1711 2065 179
Size: 1532 354 2691
Fragments arranged by size:
2691 1532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 178 811 2160 2293 2752 2961 178
Size: 633 1349 133 459 209 1794
Fragments arranged by size:
1794 1349 633 459 209 133
TatI w'GTAC_w
Cuts at: 146 4092 146
Size: 3946 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 4094 4126 157
Size: 32 3905 32 608
Fragments arranged by size:
3905 608 32 32
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 1608 1608
Size: 4577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1320 1500 2072 1320
Size: 180 572 3825
Fragments arranged by size:
3825 572 180
XmaI C'CCGG_G
Cuts at: 237 1282 1597 237
Size: 1045 315 3217
Fragments arranged by size:
3217 1045 315
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI