Restriction map for
pF1KB3574
(Circular) (Six-base) MAPSORT of: pF1KB3574.seq Check: 2426 from: 1 to: 4409
pF1KB3574 4409 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 895 895
Size: 4409
AccI GT'mk_AC
Cuts at: 776 1447 776
Size: 671 3738
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
AflIII A'CryG_T
Cuts at: 1761 2865 3842 1761
Size: 1104 977 2328
Fragments arranged by size:
2328 1104 977
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 163 1409 163
Size: 1246 3163
AlwNI CAG_nnn'CTG
Cuts at: 643 1011 1714 3281 643
Size: 368 703 1567 1771
Fragments arranged by size:
1771 1567 703 368
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 159 316 363 1413 3762 4018 159
Size: 157 47 1050 2349 256 550
Fragments arranged by size:
2349 1050 550 256 157 47
AseI AT'TA_AT
Cuts at: 20 541 20
Size: 521 3888
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 414 248 35
BanII G_rGCy'C
Cuts at: 755 872 1423 2453 755
Size: 117 551 1030 2711
Fragments arranged by size:
2711 1030 551 117
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 170 679 170
Size: 509 3900
BbvCI CC'TCA_GC
Cuts at: 599 599
Size: 4409
BciVI GTATCCnnnnn_n'
Cuts at: 1058 2307 3068 4409 1058
Size: 1249 761 1341 1058
Fragments arranged by size:
1341 1249 1058 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BfrBI ATG'CAT
Cuts at: 505 1228 505
Size: 723 3686
BglII A'GATC_T
Cuts at: 1924 3961 1924
Size: 2037 2372
BlpI GC'TnA_GC
Cuts at: 740 1521 740
Size: 781 3628
Bme1580I G_kGCm'C
Cuts at: 2034 2127 3183 2034
Size: 93 1056 3260
Fragments arranged by size:
3260 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 210 1621 1702 2037 4181 210
Size: 1411 81 335 2144 438
Fragments arranged by size:
2144 1411 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 665
Size: 4409
Bpu10I CC'TnA_GC
Cuts at: 323 599 323
Size: 276 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2956 3254 1585
Size: 879 492 298 2740
Fragments arranged by size:
2740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4113 4113
Size: 4409
BsaAI yAC'GTr
Cuts at: 1122 1762 1122
Size: 640 3769
BsaBI GATnn'nnATC
Cuts at: 6 727 1923 3960 6
Size: 721 1196 2037 455
Fragments arranged by size:
2037 1196 721 455
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 185
Size: 4409
BseYI C'CCAG_C
Cuts at: 1011 1130 1834 2592 3169 3665 1011
Size: 119 704 758 577 496 1755
Fragments arranged by size:
1755 758 704 577 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 986
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 476 1423 2201 2391 3183 476
Size: 947 778 190 792 1702
Fragments arranged by size:
1702 947 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 476 755 872 1423 2034 2127 2201 2391 2453
Size: 279 117 551 611 93 74 190 62
Cuts at: 2453 3183 476
Size: 730 1702
Fragments arranged by size:
1702 730 611 551 279 190 117 93
74 62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 640 895 1459 1673 1975 2356 640
Size: 255 564 214 302 381 2693
Fragments arranged by size:
2693 564 381 302 255 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1376 2321 1376
Size: 945 3464
BsrFI r'CCGG_y
Cuts at: 2407 2588 2797 2407
Size: 181 209 4019
Fragments arranged by size:
4019 209 181
BssSI C'ACGA_G
Cuts at: 475 2680 3038 475
Size: 2205 358 1846
Fragments arranged by size:
2205 1846 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1786 1786
Size: 4409
BstBI TT'CG_AA
Cuts at: 367 902 2770 367
Size: 535 1868 2006
Fragments arranged by size:
2006 1868 535
BstXI CCAn_nnnn'nTGG
Cuts at: 933 1134 933
Size: 201 4208
BstYI r'GATC_y
Cuts at: 1 527 1434 1901 1924 2259 2505 3517 3603
Size: 526 907 467 23 335 246 1012 86
Cuts at: 3603 3615 3961 1
Size: 12 346 449
Fragments arranged by size:
1012 907 526 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 353 107
Size: 246 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 128 741 1652 128
Size: 613 911 2885
Fragments arranged by size:
2885 911 613
ClaI AT'CG_AT
Cuts at: 1391 1391
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DraIII CAC_nnn'GTG
Cuts at: 1113 1113
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 174 2115 2973 174
Size: 1941 858 1610
Fragments arranged by size:
1941 1610 858
EaeI y'GGCC_r
Cuts at: 1124 1214 1994 2168 2559 2586 3931 1124
Size: 90 780 174 391 27 1345 1602
Fragments arranged by size:
1602 1345 780 391 174 90 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2927 3073 4219 2617
Size: 310 146 1146 2807
Fragments arranged by size:
2807 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1421 1421
Size: 4409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 2234 2666 3413 3681 665
Size: 1569 432 747 268 1393
Fragments arranged by size:
1569 1393 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 637 637
Size: 4409
EcoO109I rG'GnC_Cy
Cuts at: 114 1098 1548 114
Size: 984 450 2975
Fragments arranged by size:
2975 984 450
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 180 212 2251 2281 2283 2313 180
Size: 32 2039 30 2 30 2276
Fragments arranged by size:
2276 2039 32 30 30 2
HincII GTy'rAC
Cuts at: 429 896 1448 429
Size: 467 552 3390
Fragments arranged by size:
3390 552 467
HpaI GTT'AAC
Cuts at: 896 896
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1128 1382 3080 3264 1128
Size: 254 1698 184 2273
Fragments arranged by size:
2273 1698 254 184
MscI TGG'CCA
Cuts at: 1126 1216 2170 1126
Size: 90 954 3365
Fragments arranged by size:
3365 954 90
MslI CAynn'nnrTG
Cuts at: 1391 2398 2525 1391
Size: 1007 127 3275
Fragments arranged by size:
3275 1007 127
MspA1I CmG'CkG
Cuts at: 1134 1520 1834 2194 3207 3452 1134
Size: 386 314 360 1013 245 2091
Fragments arranged by size:
2091 1013 386 360 314 245
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NcoI C'CATG_G
Cuts at: 107 353 107
Size: 246 4163
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NsiI A_TGCA'T
Cuts at: 507 1230 507
Size: 723 3686
NspI r_CATG'y
Cuts at: 748 1462 1962 2869 748
Size: 714 500 907 2288
Fragments arranged by size:
2288 907 714 500
PacI TTA_AT'TAA
Cuts at: 545 545
Size: 4409
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PflMI CCAn_nnn'nTGG
Cuts at: 439 439
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PmlI CAC'GTG
Cuts at: 1122 1122
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 4377
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3724
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 1134 1834 2194 1134
Size: 700 360 3349
Fragments arranged by size:
3349 700 360
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1423 1423
Size: 4409
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SfcI C'TryA_G
Cuts at: 33 608 1452 2137 3130 3321 33
Size: 575 844 685 993 191 1121
Fragments arranged by size:
1121 993 844 685 575 191
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 1564 2479 2971 3233 1564
Size: 915 492 262 2740
Fragments arranged by size:
2740 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 748 1462 1962 748
Size: 714 500 3195
Fragments arranged by size:
3195 714 500
StyI C'CwwG_G
Cuts at: 107 353 1543 1897 107
Size: 246 1190 354 2619
Fragments arranged by size:
2619 1190 354 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1144 1992 2125 2584 2793 1144
Size: 848 133 459 209 2760
Fragments arranged by size:
2760 848 459 209 133
TatI w'GTAC_w
Cuts at: 3924 3924
Size: 4409
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 720 752 3926 3958 720
Size: 32 3174 32 1171
Fragments arranged by size:
3174 1171 32 32
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1904 114
Size: 1790 2619
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
XmnI GAAnn'nnTTC
Cuts at: 1315 1315
Size: 4409
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI