Restriction map for
pF1KB3547
(Circular) (Six-base) MAPSORT of: pF1KB3547.seq Check: 5005 from: 1 to: 6386
pF1KB3547 6386 bp
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AccI GT'mk_AC
Cuts at: 310 476 3424 310
Size: 166 2948 3272
Fragments arranged by size:
3272 2948 166
Acc65I G'GTAC_C
Cuts at: 3402 3402
Size: 6386
AclI AA'CG_TT
Cuts at: 6226 6226
Size: 6386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2035 4211 4643 5390 5658 1672
Size: 363 2176 432 747 268 2400
Fragments arranged by size:
2400 2176 747 432 363 268
AflIII A'CryG_T
Cuts at: 1254 3139 3738 4842 5819 1254
Size: 1885 599 1104 977 1821
Fragments arranged by size:
1885 1821 1104 977 599
AgeI A'CCGG_T
Cuts at: 4774 4774
Size: 6386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 6354
AlwNI CAG_nnn'CTG
Cuts at: 3691 5258 3691
Size: 1567 4819
ApaLI G'TGCA_C
Cuts at: 5156 5156
Size: 6386
ApoI r'AATT_y
Cuts at: 1343 2104 2115 2526 2541 3390 5739 5995 1343
Size: 761 11 411 15 849 2349 256 1734
Fragments arranged by size:
2349 1734 849 761 411 256 15 11
AseI AT'TA_AT
Cuts at: 20 2177 20
Size: 2157 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
AvaI C'yCGr_G
Cuts at: 3406 3406
Size: 6386
BamHI G'GATC_C
Cuts at: 3411 3411
Size: 6386
BanI G'GyrC_C
Cuts at: 2428 3402 3816 4064 4099 2428
Size: 974 414 248 35 4715
Fragments arranged by size:
4715 974 414 248 35
BanII G_rGCy'C
Cuts at: 634 1215 2789 3400 4430 634
Size: 581 1574 611 1030 2590
Fragments arranged by size:
2590 1574 1030 611 581
BbeI G_GCGC'C
Cuts at: 3820 4068 3820
Size: 248 6138
BbsI GAAGACnn'nnnn_
Cuts at: 239 710 834 1407 1497 239
Size: 471 124 573 90 5128
Fragments arranged by size:
5128 573 471 124 90
BciVI GTATCCnnnnn_n'
Cuts at: 4284 5045 6386 4284
Size: 761 1341 4284
Fragments arranged by size:
4284 1341 761
BclI T'GATC_A
Cuts at: 3906 3906
Size: 6386
BfrBI ATG'CAT
Cuts at: 1465 1465
Size: 6386
BglII A'GATC_T
Cuts at: 385 3901 5938 385
Size: 3516 2037 833
Fragments arranged by size:
3516 2037 833
BlpI GC'TnA_GC
Cuts at: 1430 2823 3498 1430
Size: 1393 675 4318
Fragments arranged by size:
4318 1393 675
Bme1580I G_kGCm'C
Cuts at: 1630 4011 4104 5160 1630
Size: 2381 93 1056 2856
Fragments arranged by size:
2856 2381 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1483 3003 3598 3679 4014 6158 1483
Size: 1520 595 81 335 2144 1711
Fragments arranged by size:
2144 1711 1520 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4753 4753
Size: 6386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 2336 2250
Size: 86 6300
Bpu10I CC'TnA_GC
Cuts at: 1001 1001
Size: 6386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2931 2956 3562 4441 4933 5231 1817
Size: 1114 25 606 879 492 298 2972
Fragments arranged by size:
2972 1114 879 606 492 298 25
BsaI GGTCTCn'nnnn_
Cuts at: 6090 6090
Size: 6386
BsaAI yAC'GTr
Cuts at: 1255 3739 1255
Size: 2484 3902
BsaBI GATnn'nnATC
Cuts at: 6 500 3900 5937 6
Size: 494 3400 2037 455
Fragments arranged by size:
3400 2037 494 455
BsaHI Gr'CG_yC
Cuts at: 3817 4065 4767 6263 3817
Size: 248 702 1496 3940
Fragments arranged by size:
3940 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1500 3575 4096 4774 5048 5195 5606 1500
Size: 2075 521 678 274 147 411 2280
Fragments arranged by size:
2280 2075 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 1565 1595 169
Size: 30 1366 30 4960
Fragments arranged by size:
4960 1366 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 1214 193
Size: 1021 5365
BseYI C'CCAG_C
Cuts at: 2710 3811 4569 5146 5642 2710
Size: 1101 758 577 496 3454
Fragments arranged by size:
3454 1101 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2780
Size: 6386
BsiEI CG_ry'CG
Cuts at: 104 197 3928 3974 5182 104
Size: 93 3731 46 1208 1308
Fragments arranged by size:
3731 1308 1208 93 46
BsiHKAI G_wGCw'C
Cuts at: 3400 4178 4368 5160 3400
Size: 778 190 792 4626
Fragments arranged by size:
4626 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 634 1215 1630 2789 3400 4011 4104 4178 4368
Size: 581 415 1159 611 611 93 74 190
Cuts at: 4368 4430 5160 634
Size: 62 730 1860
Fragments arranged by size:
1860 1159 730 611 611 581 415 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5606 5606
Size: 6386
BspHI T'CATG_A
Cuts at: 2661 5562 2661
Size: 2901 3485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 714 2505 3436 3650 3952 4333 714
Size: 1791 931 214 302 381 2767
Fragments arranged by size:
2767 1791 931 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4678 4732 6216 6381 50
Size: 4628 54 1484 165 55
Fragments arranged by size:
4628 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 471 1002 2079 4298 471
Size: 531 1077 2219 2559
Fragments arranged by size:
2559 2219 1077 531
BsrFI r'CCGG_y
Cuts at: 1516 4384 4565 4774 1516
Size: 2868 181 209 3128
Fragments arranged by size:
3128 2868 209 181
BssSI C'ACGA_G
Cuts at: 4657 5015 4657
Size: 358 6028
BstAPI GCAn_nnn'nTGC
Cuts at: 3763 3763
Size: 6386
BstBI TT'CG_AA
Cuts at: 1773 4747 1773
Size: 2974 3412
BstYI r'GATC_y
Cuts at: 1 385 2565 3411 3878 3901 4236 4482 5494
Size: 384 2180 846 467 23 335 246 1012
Cuts at: 5494 5580 5592 5938 1
Size: 86 12 346 449
Fragments arranged by size:
2180 1012 846 467 449 384 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 311 311
Size: 6386
BtgI C'CryG_G
Cuts at: 107 772 832 107
Size: 665 60 5661
Fragments arranged by size:
5661 665 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2803 4336 4517 4680 2803
Size: 1533 181 163 4509
Fragments arranged by size:
4509 1533 181 163
BtsI GCAGTG_nn'
Cuts at: 741 2396 2873 3629 741
Size: 1655 477 756 3498
Fragments arranged by size:
3498 1655 756 477
ClaI AT'CG_AT
Cuts at: 1652 1652
Size: 6386
DraI TTT'AAA
Cuts at: 2114 3385 2114
Size: 1271 5115
DrdI GACnn_nn'nnGTC
Cuts at: 4092 4950 4092
Size: 858 5528
EaeI y'GGCC_r
Cuts at: 194 1518 3971 4145 4536 4563 5908 194
Size: 1324 2453 174 391 27 1345 672
Fragments arranged by size:
2453 1345 1324 672 391 174 27
EagI C'GGCC_G
Cuts at: 194 3971 194
Size: 3777 2609
EarI CTCTTCn'nnn_
Cuts at: 1199 1502 1952 4409 4619 1199
Size: 303 450 2457 210 2966
Fragments arranged by size:
2966 2457 450 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4594 4904 5050 6196 4594
Size: 310 146 1146 4784
Fragments arranged by size:
4784 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3398 3398
Size: 6386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2035 2250 2336 4211 4643 5390 5658 1672
Size: 363 215 86 1875 432 747 268 2400
Fragments arranged by size:
2400 1875 747 432 363 268 215 86
EcoO109I rG'GnC_Cy
Cuts at: 1297 3235 3525 1297
Size: 1938 290 4158
Fragments arranged by size:
4158 1938 290
EcoRI G'AATT_C
Cuts at: 3390 3390
Size: 6386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 253 886 1131 1557 2503 4405 253
Size: 633 245 426 946 1902 2234
Fragments arranged by size:
2234 1902 946 633 426 245
FspI TGC'GCA
Cuts at: 4167 4167
Size: 6386
HaeII r_GCGC'y
Cuts at: 3820 4068 5090 6069 3820
Size: 248 1022 979 4137
Fragments arranged by size:
4137 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1953 1985 2643 2675 2758 2790 3019 3051 4228
Size: 32 658 32 83 32 229 32 1177
Cuts at: 4228 4258 4260 4290 1953
Size: 30 2 30 4049
Fragments arranged by size:
4049 1177 658 229 83 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1279 3425 1279
Size: 2146 4240
HindIII A'AGCT_T
Cuts at: 3091 3091
Size: 6386
KasI G'GCGC_C
Cuts at: 3816 4064 3816
Size: 248 6138
KpnI G_GTAC'C
Cuts at: 3406 3406
Size: 6386
MfeI C'AATT_G
Cuts at: 638 638
Size: 6386
MluI A'CGCG_T
Cuts at: 5819 5819
Size: 6386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 155 2487 5057 5241 155
Size: 2332 2570 184 1300
Fragments arranged by size:
2570 2332 1300 184
MscI TGG'CCA
Cuts at: 4147 4147
Size: 6386
MslI CAynn'nnrTG
Cuts at: 717 1286 1872 3144 4375 4502 717
Size: 569 586 1272 1231 127 2601
Fragments arranged by size:
2601 1272 1231 586 569 127
MspA1I CmG'CkG
Cuts at: 339 1137 1554 3497 3811 4171 5184 5429 339
Size: 798 417 1943 314 360 1013 245 1296
Fragments arranged by size:
1943 1296 1013 798 417 360 314 245
NaeI GCC'GGC
Cuts at: 4567 4567
Size: 6386
NarI GG'CG_CC
Cuts at: 3817 4065 3817
Size: 248 6138
NcoI C'CATG_G
Cuts at: 107 772 832 107
Size: 665 60 5661
Fragments arranged by size:
5661 665 60
NdeI CA'TA_TG
Cuts at: 2346 2829 2346
Size: 483 5903
NgoMIV G'CCGG_C
Cuts at: 4565 4565
Size: 6386
NruI TCG'CGA
Cuts at: 698 698
Size: 6386
NsiI A_TGCA'T
Cuts at: 1467 1467
Size: 6386
NspI r_CATG'y
Cuts at: 870 3143 3439 3939 4846 870
Size: 2273 296 500 907 2410
Fragments arranged by size:
2410 2273 907 500 296
PciI A'CATG_T
Cuts at: 3139 4842 3139
Size: 1703 4683
PfoI T'CCnGG_A
Cuts at: 2530 2530
Size: 6386
PmeI GTTT'AAAC
Cuts at: 3385 3385
Size: 6386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 169 201 5525 5557 169
Size: 32 5324 32 998
Fragments arranged by size:
5324 998 32 32
PpuMI rG'GwC_Cy
Cuts at: 1297 1297
Size: 6386
PshAI GACnn'nnGTC
Cuts at: 1882 1882
Size: 6386
PsiI TTA'TAA
Cuts at: 1106 1148 1106
Size: 42 6344
PstI C_TGCA'G
Cuts at: 2502 3433 4118 2502
Size: 931 685 4770
Fragments arranged by size:
4770 931 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6386
PvuII CAG'CTG
Cuts at: 339 1137 1554 3811 4171 339
Size: 798 417 2257 360 2554
Fragments arranged by size:
2554 2257 798 417 360
RsrII CG'GwC_CG
Cuts at: 4581 4581
Size: 6386
SacI G_AGCT'C
Cuts at: 3400 3400
Size: 6386
SalI G'TCGA_C
Cuts at: 3423 3423
Size: 6386
SapI GCTCTTCn'nnn_
Cuts at: 4409 4619 4409
Size: 210 6176
SbfI CC_TGCA'GG
Cuts at: 3433 3433
Size: 6386
ScaI AGT'ACT
Cuts at: 5903 5903
Size: 6386
SexAI A'CCwGG_T
Cuts at: 2424 2726 2424
Size: 302 6084
SfcI C'TryA_G
Cuts at: 33 1926 2462 2498 2896 3429 4114 5107 5298
Size: 1893 536 36 398 533 685 993 191
Cuts at: 5298 33
Size: 1121
Fragments arranged by size:
1893 1121 993 685 536 533 398 191
36
SfoI GGC'GCC
Cuts at: 3818 4066 3818
Size: 248 6138
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
SmaI CCC'GGG
Cuts at: 3408 3408
Size: 6386
SmlI C'TyrA_G
Cuts at: 1832 2910 2935 3541 4456 4948 5210 1832
Size: 1078 25 606 915 492 262 3008
Fragments arranged by size:
3008 1078 915 606 492 262 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6386
SphI G_CATG'C
Cuts at: 3439 3939 3439
Size: 500 5886
SspI AAT'ATT
Cuts at: 1384 3113 1384
Size: 1729 4657
StyI C'CwwG_G
Cuts at: 107 772 832 843 1910 3520 3874 107
Size: 665 60 11 1067 1610 354 2619
Fragments arranged by size:
2619 1610 1067 665 354 60 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 3969 4102 4561 4770 204
Size: 3765 133 459 209 1820
Fragments arranged by size:
3765 1820 459 209 133
TatI w'GTAC_w
Cuts at: 5901 5901
Size: 6386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1302 1334 1565 1597 5903 5935 1302
Size: 32 231 32 4306 32 1753
Fragments arranged by size:
4306 1753 231 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 238 4183 238
Size: 3945 2441
XbaI T'CTAG_A
Cuts at: 3417 3417
Size: 6386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 770 1558 3881 770
Size: 788 2323 3275
Fragments arranged by size:
3275 2323 788
XmaI C'CCGG_G
Cuts at: 3406 3406
Size: 6386
XmnI GAAnn'nnTTC
Cuts at: 3066 3066
Size: 6386
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NheI
NotI PacI PasI PflMI PmlI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI