Restriction map for
pF1KB3491
(Circular) (Six-base) MAPSORT of: pF1KB3491.seq Check: 721 from: 1 to: 5147
pF1KB3491 5147 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1681 1737 1681
Size: 56 5091
AccI GT'mk_AC
Cuts at: 2185 2185
Size: 5147
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5147
AclI AA'CG_TT
Cuts at: 4987 4987
Size: 5147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1012 2972 3404 3757 4419 932
Size: 80 1960 432 353 662 1660
Fragments arranged by size:
1960 1660 662 432 353 80
AfeI AGC'GCT
Cuts at: 232 232
Size: 5147
AflIII A'CryG_T
Cuts at: 1650 2499 4299 4580 1650
Size: 849 1800 281 2217
Fragments arranged by size:
2217 1800 849 281
AhdI GACnn_n'nnGTC
Cuts at: 1501 1501
Size: 5147
AleI CACnn'nnGTG
Cuts at: 1135 2015 1135
Size: 880 4267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1769 1769
Size: 5147
AlwNI CAG_nnn'CTG
Cuts at: 576 724 1114 2452 3890 576
Size: 148 390 1338 1438 1833
Fragments arranged by size:
1833 1438 1338 390 148
ApaI G_GGCC'C
Cuts at: 1826 1826
Size: 5147
ApaLI G'TGCA_C
Cuts at: 3985 3985
Size: 5147
ApoI r'AATT_y
Cuts at: 446 499 553 862 1354 1535 2062 2083 2151
Size: 53 54 309 492 181 527 21 68
Cuts at: 2151 4500 4756 446
Size: 2349 256 837
Fragments arranged by size:
2349 837 527 492 309 256 181 68
54 53 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 5147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
AvaI C'yCGr_G
Cuts at: 1853 2167 1853
Size: 314 4833
BamHI G'GATC_C
Cuts at: 141 2172 141
Size: 2031 3116
BanI G'GyrC_C
Cuts at: 1555 2163 2577 2825 2860 1555
Size: 608 414 248 35 3842
Fragments arranged by size:
3842 608 414 248 35
BanII G_rGCy'C
Cuts at: 1108 1826 2161 3191 1108
Size: 718 335 1030 3064
Fragments arranged by size:
3064 1030 718 335
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4899
BbsI GAAGACnn'nnnn_
Cuts at: 224 1165 224
Size: 941 4206
BbvCI CC'TCA_GC
Cuts at: 995 1827 995
Size: 832 4315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 758 792 758
Size: 34 5113
BciVI GTATCCnnnnn_n'
Cuts at: 1151 2014 3045 4101 5147 1151
Size: 863 1031 1056 1046 1151
Fragments arranged by size:
1151 1056 1046 1031 863
BclI T'GATC_A
Cuts at: 300 2007 2667 300
Size: 1707 660 2780
Fragments arranged by size:
2780 1707 660
BfrBI ATG'CAT
Cuts at: 769 769
Size: 5147
BglI GCCn_nnn'nGGC
Cuts at: 991 1065 991
Size: 74 5073
BglII A'GATC_T
Cuts at: 676 1149 2662 4699 676
Size: 473 1513 2037 1124
Fragments arranged by size:
2037 1513 1124 473
BlpI GC'TnA_GC
Cuts at: 2259 2259
Size: 5147
Bme1580I G_kGCm'C
Cuts at: 1826 2772 2865 3989 1826
Size: 946 93 1124 2984
Fragments arranged by size:
2984 1124 946 93
BmrI ACTGGGnnnn_n'
Cuts at: 498 1869 2359 2440 2775 4919 498
Size: 1371 490 81 335 2144 726
Fragments arranged by size:
2144 1371 726 490 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 553 746 1054 1136 1292 1424 1436 1499
Size: 203 193 308 82 156 132 12 63
Cuts at: 1499 1700 1894 350
Size: 201 194 3603
Fragments arranged by size:
3603 308 203 201 194 193 156 132
82 63 12
Bpu10I CC'TnA_GC
Cuts at: 995 1827 995
Size: 832 4315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 3202 3916 4214 2323
Size: 879 714 298 3256
Fragments arranged by size:
3256 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4851 4851
Size: 5147
BsaAI yAC'GTr
Cuts at: 1653 2500 1653
Size: 847 4300
BsaBI GATnn'nnATC
Cuts at: 6 2661 4698 6
Size: 2655 2037 455
Fragments arranged by size:
2655 2037 455
BsaHI Gr'CG_yC
Cuts at: 2578 2826 3528 5024 2578
Size: 248 702 1496 2701
Fragments arranged by size:
2701 1496 702 248
BsaWI w'CCGG_w
Cuts at: 527 2336 2857 3535 3946 4093 4367 527
Size: 1809 521 678 411 147 274 1307
Fragments arranged by size:
1809 1307 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1195 1225 1195
Size: 30 5117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 718 718
Size: 5147
BseYI C'CCAG_C
Cuts at: 1698 2572 3330 3995 4403 1698
Size: 874 758 665 408 2442
Fragments arranged by size:
2442 874 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 298 142
Size: 156 4991
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3965 104
Size: 2585 46 1230 1286
Fragments arranged by size:
2585 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 366 1523 2161 2939 3129 3989 366
Size: 1157 638 778 190 860 1524
Fragments arranged by size:
1524 1157 860 778 638 190
BsmI GAATG_Cn'
Cuts at: 1172 1172
Size: 5147
Bsp1286I G_dGCh'C
Cuts at: 366 1108 1523 1826 2161 2772 2865 2939 3129
Size: 742 415 303 335 611 93 74 190
Cuts at: 3129 3191 3989 366
Size: 62 798 1524
Fragments arranged by size:
1524 798 742 611 415 335 303 190
93 74 62
BspHI T'CATG_A
Cuts at: 147 3579 147
Size: 3432 1715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1681 1737 2197 2411 2713 3094 1681
Size: 56 460 214 302 381 3734
Fragments arranged by size:
3734 460 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 3439 3493 4977 5142 50
Size: 3389 54 1484 165 55
Fragments arranged by size:
3389 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1617 1979 3059 1617
Size: 362 1080 3705
Fragments arranged by size:
3705 1080 362
BsrFI r'CCGG_y
Cuts at: 3145 3326 3145
Size: 181 4966
BsrGI T'GTAC_A
Cuts at: 1211 1211
Size: 5147
BssSI C'ACGA_G
Cuts at: 3418 4126 3418
Size: 708 4439
BstAPI GCAn_nnn'nTGC
Cuts at: 2524 2524
Size: 5147
BstBI TT'CG_AA
Cuts at: 3508 3508
Size: 5147
BstEII G'GTnAC_C
Cuts at: 666 1713 666
Size: 1047 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 1446 1446
Size: 5147
BstYI r'GATC_y
Cuts at: 1 141 676 1149 2172 2639 2662 2997 3243
Size: 140 535 473 1023 467 23 335 246
Cuts at: 3243 3549 3561 3647 4699 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1023 535 473 467 449 335 306
246 140 86 23 12
BtgI C'CryG_G
Cuts at: 107 1490 1685 107
Size: 1383 195 3569
Fragments arranged by size:
3569 1383 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3097 3278 3441 3097
Size: 181 163 4803
Fragments arranged by size:
4803 181 163
BtsI GCAGTG_nn'
Cuts at: 1130 1142 2132 2390 1130
Size: 12 990 258 3887
Fragments arranged by size:
3887 990 258 12
DraI TTT'AAA
Cuts at: 1664 2146 1664
Size: 482 4665
DrdI GACnn_nn'nnGTC
Cuts at: 2853 4197 2853
Size: 1344 3803
EaeI y'GGCC_r
Cuts at: 1764 2732 2906 3297 3324 4669 1764
Size: 968 174 391 27 1345 2242
Fragments arranged by size:
2242 1345 968 391 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5147
EarI CTCTTCn'nnn_
Cuts at: 1359 3170 3380 1359
Size: 1811 210 3126
Fragments arranged by size:
3126 1811 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3355 4097 4243 4957 3355
Size: 742 146 714 3545
Fragments arranged by size:
3545 742 714 146
EcoICRI GAG'CTC
Cuts at: 2159 2159
Size: 5147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 553 746 932 1012 1054 1136 1292 1424
Size: 203 193 186 80 42 82 156 132
Cuts at: 1424 1436 1499 1700 1894 2972 3404 3757 4419
Size: 12 63 201 194 1078 432 353 662
Cuts at: 4419 350
Size: 1078
Fragments arranged by size:
1078 1078 662 432 353 203 201 194
193 186 156 132 82 80 63 42
12
EcoNI CCTnn'n_nnAGG
Cuts at: 1049 1049
Size: 5147
EcoO109I rG'GnC_Cy
Cuts at: 1001 1495 1822 1823 2286 1001
Size: 494 327 1 463 3862
Fragments arranged by size:
3862 494 463 327 1
EcoRI G'AATT_C
Cuts at: 553 2151 553
Size: 1598 3549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1260 3166 1260
Size: 1906 3241
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5147
HaeII r_GCGC'y
Cuts at: 234 957 1511 2581 2829 4059 4830 234
Size: 723 554 1070 248 1230 771 551
Fragments arranged by size:
1230 1070 771 723 554 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 627 659 719 751 1487 1519 1836
Size: 32 283 32 60 32 736 32 317
Cuts at: 1836 1868 2012 2044 2989 3019 3021 3051 312
Size: 32 144 32 945 30 2 30 2408
Fragments arranged by size:
2408 945 736 317 283 144 60 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5147
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4899
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5147
MfeI C'AATT_G
Cuts at: 580 580
Size: 5147
MluI A'CGCG_T
Cuts at: 4580 4580
Size: 5147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 531 3906 4090 531
Size: 3375 184 1588
Fragments arranged by size:
3375 1588 184
MscI TGG'CCA
Cuts at: 1766 2908 1766
Size: 1142 4005
MslI CAynn'nnrTG
Cuts at: 1135 2015 3136 3263 1135
Size: 880 1121 127 3019
Fragments arranged by size:
3019 1121 880 127
MspA1I CmG'CkG
Cuts at: 724 1678 1702 1914 1951 2258 2572 2932 3716
Size: 954 24 212 37 307 314 360 784
Cuts at: 3716 3961 724
Size: 245 1910
Fragments arranged by size:
1910 954 784 360 314 307 245 212
37 24
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5147
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4899
NcoI C'CATG_G
Cuts at: 107 1685 107
Size: 1578 3569
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5147
NsiI A_TGCA'T
Cuts at: 771 771
Size: 5147
NspI r_CATG'y
Cuts at: 592 687 2200 2700 4303 592
Size: 95 1513 500 1603 1436
Fragments arranged by size:
1603 1513 1436 500 95
PciI A'CATG_T
Cuts at: 4299 4299
Size: 5147
PfoI T'CCnGG_A
Cuts at: 569 1523 1811 569
Size: 954 288 3905
Fragments arranged by size:
3905 954 288
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5147
PmlI CAC'GTG
Cuts at: 1653 1653
Size: 5147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 5115
PpuMI rG'GwC_Cy
Cuts at: 1495 1495
Size: 5147
PshAI GACnn'nnGTC
Cuts at: 1038 1038
Size: 5147
PspOMI G'GGCC_C
Cuts at: 1822 1822
Size: 5147
PspXI vC'TCGA_Gb
Cuts at: 1853 1853
Size: 5147
PstI C_TGCA'G
Cuts at: 1137 1748 1851 2194 2879 1137
Size: 611 103 343 685 3405
Fragments arranged by size:
3405 685 611 343 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5147
PvuII CAG'CTG
Cuts at: 724 1678 1702 2572 2932 724
Size: 954 24 870 360 2939
Fragments arranged by size:
2939 954 870 360 24
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5147
SacI G_AGCT'C
Cuts at: 2161 2161
Size: 5147
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5147
SanDI GG'GwC_CC
Cuts at: 1495 1495
Size: 5147
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4937
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5147
ScaI AGT'ACT
Cuts at: 183 4664 183
Size: 4481 666
SexAI A'CCwGG_T
Cuts at: 743 743
Size: 5147
SfcI C'TryA_G
Cuts at: 33 169 1133 1145 1272 1744 1847 2190 2875
Size: 136 964 12 127 472 103 343 685
Cuts at: 2875 3843 4034 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 964 685 472 343 191 136
127 103 12
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4899
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5147
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5147
SmlI C'TyrA_G
Cuts at: 1853 2302 3217 3931 4193 1853
Size: 449 915 714 262 2807
Fragments arranged by size:
2807 915 714 449 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5147
SphI G_CATG'C
Cuts at: 2200 2700 2200
Size: 500 4647
StuI AGG'CCT
Cuts at: 1059 1940 1059
Size: 881 4266
StyI C'CwwG_G
Cuts at: 107 308 1685 1889 2281 2635 107
Size: 201 1377 204 392 354 2619
Fragments arranged by size:
2619 1377 392 354 204 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2730 2863 3322 3531 2730
Size: 133 459 209 4346
Fragments arranged by size:
4346 459 209 133
TatI w'GTAC_w
Cuts at: 181 1211 4662 181
Size: 1030 3451 666
Fragments arranged by size:
3451 1030 666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4664 4696 4664
Size: 32 5115
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5147
XbaI T'CTAG_A
Cuts at: 2178 2178
Size: 5147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 1183 2642 381
Size: 802 1459 2886
Fragments arranged by size:
2886 1459 802
XhoI C'TCGA_G
Cuts at: 1853 1853
Size: 5147
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5147
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BaeI BmgBI
BmtI BsiWI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PflMI PsiI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI