Restriction map for
pF1KB3484
(Circular) (Six-base) MAPSORT of: pF1KB3484.seq Check: 1922 from: 1 to: 3746
pF1KB3484 3746 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1571 2003 2750 3018 472
Size: 1099 432 747 268 1200
Fragments arranged by size:
1200 1099 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AhdI GACnn_n'nnGTC
Cuts at: 479 479
Size: 3746
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaLI G'TGCA_C
Cuts at: 2516 2516
Size: 3746
ApoI r'AATT_y
Cuts at: 131 750 3099 3355 131
Size: 619 2349 256 522
Fragments arranged by size:
2349 619 522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 766 766
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 762 1176 1424 1459 762
Size: 414 248 35 3049
Fragments arranged by size:
3049 414 248 35
BanII G_rGCy'C
Cuts at: 147 760 1790 147
Size: 613 1030 2103
Fragments arranged by size:
2103 1030 613
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BbsI GAAGACnn'nnnn_
Cuts at: 325 325
Size: 3746
BciVI GTATCCnnnnn_n'
Cuts at: 514 601 1644 2405 3746 514
Size: 87 1043 761 1341 514
Fragments arranged by size:
1341 1043 761 514 87
BclI T'GATC_A
Cuts at: 1266 1266
Size: 3746
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 1371 1464 2520 1371
Size: 93 1056 2597
Fragments arranged by size:
2597 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 922 1801 2293 2591 563
Size: 359 879 492 298 1718
Fragments arranged by size:
1718 879 492 359 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 1177 1425 2127 3623 1177
Size: 248 702 1496 1300
Fragments arranged by size:
1496 1300 702 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 195 195
Size: 3746
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 147 760 1538 1728 2520 147
Size: 613 778 190 792 1373
Fragments arranged by size:
1373 792 778 613 190
Bsp1286I G_dGCh'C
Cuts at: 147 760 1371 1464 1538 1728 1790 2520 147
Size: 613 611 93 74 190 62 730 1373
Fragments arranged by size:
1373 730 613 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 1010 1312 1693 796
Size: 214 302 381 2849
Fragments arranged by size:
2849 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 450 636 1658 450
Size: 186 1022 2538
Fragments arranged by size:
2538 1022 186
BsrFI r'CCGG_y
Cuts at: 1744 1925 2134 1744
Size: 181 209 3356
Fragments arranged by size:
3356 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3746
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1696 1877 2040 1696
Size: 181 163 3402
Fragments arranged by size:
3402 181 163
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 327 362 327
Size: 35 3711
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 1331 1505 1896 1923 3268 1331
Size: 174 391 27 1345 1809
Fragments arranged by size:
1809 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 560 1769 1979 560
Size: 1209 210 2327
Fragments arranged by size:
2327 1209 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1954 2264 2410 3556 1954
Size: 310 146 1146 2144
Fragments arranged by size:
2144 1146 310 146
EcoICRI GAG'CTC
Cuts at: 145 758 145
Size: 613 3133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1571 2003 2750 3018 472
Size: 1099 432 747 268 1200
Fragments arranged by size:
1200 1099 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 885 885
Size: 3746
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 1180 1428 2450 3429 1180
Size: 248 1022 979 1497
Fragments arranged by size:
1497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 465 497 627 659 1588 1618 1620
Size: 32 330 32 130 32 929 30 2
Cuts at: 1620 1650 103
Size: 30 2199
Fragments arranged by size:
2199 929 330 130 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 235 1735 1862 235
Size: 1500 127 2119
Fragments arranged by size:
2119 1500 127
MspA1I CmG'CkG
Cuts at: 857 1171 1531 2544 2789 857
Size: 314 360 1013 245 1814
Fragments arranged by size:
1814 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1927 1927
Size: 3746
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NcoI C'CATG_G
Cuts at: 107 107
Size: 3746
NgoMIV G'CCGG_C
Cuts at: 1925 1925
Size: 3746
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2917 2885
Size: 32 3714
PsiI TTA'TAA
Cuts at: 404 404
Size: 3746
PstI C_TGCA'G
Cuts at: 793 1478 793
Size: 685 3061
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 147 760 147
Size: 613 3133
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 1769 1979 1769
Size: 210 3536
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 789 1474 2467 2658 33
Size: 756 685 993 191 1121
Fragments arranged by size:
1121 993 756 685 191
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SmaI CCC'GGG
Cuts at: 768 768
Size: 3746
SmlI C'TyrA_G
Cuts at: 578 901 1816 2308 2570 578
Size: 323 915 492 262 1754
Fragments arranged by size:
1754 915 492 323 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 107 880 1234 107
Size: 773 354 2619
Fragments arranged by size:
2619 773 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 777 777
Size: 3746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 1241 237
Size: 1004 2742
XmaI C'CCGG_G
Cuts at: 766 766
Size: 3746
XmnI GAAnn'nnTTC
Cuts at: 131 131
Size: 3746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI