Restriction map for
pF1KB3477
(Circular) (Six-base) MAPSORT of: pF1KB3477.seq Check: 1826 from: 1 to: 3716
pF1KB3477 3716 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 377 377
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AfeI AGC'GCT
Cuts at: 313 313
Size: 3716
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 571 603 571
Size: 32 3684
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 517 720 3069 3325 517
Size: 203 2349 256 908
Fragments arranged by size:
2349 908 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 113 736 113
Size: 623 3093
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 287 1231 3268 287
Size: 944 2037 735
Fragments arranged by size:
2037 944 735
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2490 1341
Size: 93 1056 2567
Fragments arranged by size:
2567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
Bpu10I CC'TnA_GC
Cuts at: 482 482
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 544 3420 544
Size: 2876 840
BsaAI yAC'GTr
Cuts at: 335 1069 335
Size: 734 2982
BsaBI GATnn'nnATC
Cuts at: 6 535 1230 3267 6
Size: 529 695 2037 455
Fragments arranged by size:
2037 695 529 455
BsaHI Gr'CG_yC
Cuts at: 374 1147 1395 2097 3593 374
Size: 773 248 702 1496 497
Fragments arranged by size:
1496 773 702 497 248
BsaWI w'CCGG_w
Cuts at: 248 905 1426 2104 2378 2525 2936 248
Size: 657 521 678 274 147 411 1028
Fragments arranged by size:
1028 678 657 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 230 1258 1304 2512 104
Size: 126 1028 46 1208 1308
Fragments arranged by size:
1308 1208 1028 126 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 493 493
Size: 3716
Bsp1286I G_dGCh'C
Cuts at: 730 1341 1434 1508 1698 1760 2490 730
Size: 611 93 74 190 62 730 1956
Fragments arranged by size:
1956 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 248 2936 248
Size: 2688 1028
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 486 2008 2062 3546 3711 50
Size: 436 1522 54 1484 165 55
Fragments arranged by size:
1522 1484 436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 245 287 741 1208 1231 1566 1812 2824
Size: 244 42 454 467 23 335 246 1012
Cuts at: 2824 2910 2922 3268 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 454 449 346 335 246 244
86 42 23 12
BtgI C'CryG_G
Cuts at: 107 254 351 107
Size: 147 97 3472
Fragments arranged by size:
3472 147 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3238 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 136 575 1739 1949 136
Size: 439 1164 210 1903
Fragments arranged by size:
1903 1164 439 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 347 855 347
Size: 508 3208
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 315 560 1150 1398 2420 3399 315
Size: 245 590 248 1022 979 632
Fragments arranged by size:
1022 979 632 590 248 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 399 573 755 399
Size: 174 182 3360
Fragments arranged by size:
3360 182 174
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 1705 1832 1705
Size: 127 3589
MspA1I CmG'CkG
Cuts at: 158 301 827 1141 1501 2514 2759 158
Size: 143 526 314 360 1013 245 1115
Fragments arranged by size:
1115 1013 526 360 314 245 143
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 107 107
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PpuMI rG'GwC_Cy
Cuts at: 347 347
Size: 3716
PstI C_TGCA'G
Cuts at: 287 763 1448 287
Size: 476 685 2555
Fragments arranged by size:
2555 685 476
PvuI CG_AT'CG
Cuts at: 104 230 104
Size: 126 3590
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 251 1911 251
Size: 1660 2056
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SanDI GG'GwC_CC
Cuts at: 347 347
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 283 759 1444 2437 2628 33
Size: 250 476 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 250 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 115 738 115
Size: 623 3093
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
SspI AAT'ATT
Cuts at: 455 455
Size: 3716
StyI C'CwwG_G
Cuts at: 107 560 850 1204 107
Size: 453 290 354 2619
Fragments arranged by size:
2619 453 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 1211 231
Size: 980 2736
XmaI C'CCGG_G
Cuts at: 113 736 113
Size: 623 3093
ZraI GAC'GTC
Cuts at: 375 375
Size: 3716
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI