Restriction map for
pF1KB3467
(Circular) (Six-base) MAPSORT of: pF1KB3467.seq Check: 1175 from: 1 to: 5099
pF1KB3467 5099 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1086 1086
Size: 5099
AccI GT'mk_AC
Cuts at: 2137 2137
Size: 5099
Acc65I G'GTAC_C
Cuts at: 2115 2115
Size: 5099
AclI AA'CG_TT
Cuts at: 2093 4939 2093
Size: 2846 2253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 345 1929 2924 3356 3709 4371 161
Size: 184 1584 995 432 353 662 889
Fragments arranged by size:
1584 995 889 662 432 353 184
AflIII A'CryG_T
Cuts at: 1641 1707 2451 4251 4532 1641
Size: 66 744 1800 281 2208
Fragments arranged by size:
2208 1800 744 281 66
AhdI GACnn_n'nnGTC
Cuts at: 1389 1389
Size: 5099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 749 749
Size: 5099
AlwNI CAG_nnn'CTG
Cuts at: 485 790 1281 2404 3842 485
Size: 305 491 1123 1438 1742
Fragments arranged by size:
1742 1438 1123 491 305
ApaLI G'TGCA_C
Cuts at: 3937 3937
Size: 5099
ApoI r'AATT_y
Cuts at: 460 970 2103 4452 4708 460
Size: 510 1133 2349 256 851
Fragments arranged by size:
2349 1133 851 510 256
AseI AT'TA_AT
Cuts at: 20 581 20
Size: 561 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
AvaI C'yCGr_G
Cuts at: 136 2119 136
Size: 1983 3116
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1494 1527 1494
Size: 33 5066
BamHI G'GATC_C
Cuts at: 2124 2124
Size: 5099
BanI G'GyrC_C
Cuts at: 890 1015 1900 1927 2115 2529 2777 2812 890
Size: 125 885 27 188 414 248 35 3177
Fragments arranged by size:
3177 885 414 248 188 125 35 27
BanII G_rGCy'C
Cuts at: 2113 3143 2113
Size: 1030 4069
BbeI G_GCGC'C
Cuts at: 894 2533 2781 894
Size: 1639 248 3212
Fragments arranged by size:
3212 1639 248
BciVI GTATCCnnnnn_n'
Cuts at: 1495 2997 4053 5099 1495
Size: 1502 1056 1046 1495
Fragments arranged by size:
1502 1495 1056 1046
BclI T'GATC_A
Cuts at: 2619 2619
Size: 5099
BglI GCCn_nnn'nGGC
Cuts at: 1976 1976
Size: 5099
BglII A'GATC_T
Cuts at: 950 2614 4651 950
Size: 1664 2037 1398
Fragments arranged by size:
2037 1664 1398
BlpI GC'TnA_GC
Cuts at: 2211 2211
Size: 5099
Bme1580I G_kGCm'C
Cuts at: 780 2724 2817 3941 780
Size: 1944 93 1124 1938
Fragments arranged by size:
1944 1938 1124 93
BmgBI CAC'GTC
Cuts at: 1708 1708
Size: 5099
BmrI ACTGGGnnnn_n'
Cuts at: 584 2311 2392 2727 4871 584
Size: 1727 81 335 2144 812
Fragments arranged by size:
2144 1727 812 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3466
Size: 5099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 704 1061 1498 584
Size: 120 357 437 4185
Fragments arranged by size:
4185 437 357 120
Bpu10I CC'TnA_GC
Cuts at: 1572 1572
Size: 5099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 2275 3154 3868 4166 843
Size: 1432 879 714 298 1776
Fragments arranged by size:
1776 1432 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1447 1520 4803 1447
Size: 73 3283 1743
Fragments arranged by size:
3283 1743 73
BsaAI yAC'GTr
Cuts at: 2452 2452
Size: 5099
BsaBI GATnn'nnATC
Cuts at: 6 2613 4650 6
Size: 2607 2037 455
Fragments arranged by size:
2607 2037 455
BsaHI Gr'CG_yC
Cuts at: 891 1083 2530 2778 3480 4976 891
Size: 192 1447 248 702 1496 1014
Fragments arranged by size:
1496 1447 1014 702 248 192
BsaWI w'CCGG_w
Cuts at: 2288 2809 3487 3898 4045 4319 2288
Size: 521 678 411 147 274 3068
Fragments arranged by size:
3068 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 895 925 1203 1233 895
Size: 30 278 30 4761
Fragments arranged by size:
4761 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 218
Size: 5099
BseYI C'CCAG_C
Cuts at: 2524 3282 3947 4355 2524
Size: 758 665 408 3268
Fragments arranged by size:
3268 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1487
Size: 5099
BsiEI CG_ry'CG
Cuts at: 104 2641 2687 3917 104
Size: 2537 46 1230 1286
Fragments arranged by size:
2537 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2113 2891 3081 3941 2113
Size: 778 190 860 3271
Fragments arranged by size:
3271 860 778 190
BsiWI C'GTAC_G
Cuts at: 1699 1699
Size: 5099
Bsp1286I G_dGCh'C
Cuts at: 780 2113 2724 2817 2891 3081 3143 3941 780
Size: 1333 611 93 74 190 62 798 1938
Fragments arranged by size:
1938 1333 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2149 2363 2665 3046 2149
Size: 214 302 381 4202
Fragments arranged by size:
4202 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1571 3391 3445 4929 5094 50
Size: 1521 1820 54 1484 165 55
Fragments arranged by size:
1820 1521 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3011 3011
Size: 5099
BsrFI r'CCGG_y
Cuts at: 3097 3278 3097
Size: 181 4918
BsrGI T'GTAC_A
Cuts at: 1683 1683
Size: 5099
BssSI C'ACGA_G
Cuts at: 1286 3370 4078 1286
Size: 2084 708 2307
Fragments arranged by size:
2307 2084 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2476 2476
Size: 5099
BstBI TT'CG_AA
Cuts at: 3460 3460
Size: 5099
BstYI r'GATC_y
Cuts at: 1 325 950 1779 2124 2591 2614 2949 3195
Size: 324 625 829 345 467 23 335 246
Cuts at: 3195 3501 3513 3599 4651 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 829 625 467 449 345 335 324
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1265 1265
Size: 5099
BtgI C'CryG_G
Cuts at: 435 1521 1989 435
Size: 1086 468 3545
Fragments arranged by size:
3545 1086 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3049 3230 3393 3049
Size: 181 163 4755
Fragments arranged by size:
4755 181 163
BtsI GCAGTG_nn'
Cuts at: 128 1980 2342 128
Size: 1852 362 2885
Fragments arranged by size:
2885 1852 362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1574 1609 1574
Size: 35 5064
DraI TTT'AAA
Cuts at: 2098 2098
Size: 5099
DraIII CAC_nnn'GTG
Cuts at: 535 535
Size: 5099
DrdI GACnn_nn'nnGTC
Cuts at: 2805 4149 2805
Size: 1344 3755
EaeI y'GGCC_r
Cuts at: 1986 2684 2858 3249 3276 4621 1986
Size: 698 174 391 27 1345 2464
Fragments arranged by size:
2464 1345 698 391 174 27
EagI C'GGCC_G
Cuts at: 2684 2684
Size: 5099
EarI CTCTTCn'nnn_
Cuts at: 180 1320 1544 3122 3332 180
Size: 1140 224 1578 210 1947
Fragments arranged by size:
1947 1578 1140 224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1222 1994 3307 4049 4195 4909 1222
Size: 772 1313 742 146 714 1412
Fragments arranged by size:
1412 1313 772 742 714 146
EcoICRI GAG'CTC
Cuts at: 2111 2111
Size: 5099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 345 584 704 1061 1498 1929 2924 3356
Size: 184 239 120 357 437 431 995 432
Cuts at: 3356 3709 4371 161
Size: 353 662 889
Fragments arranged by size:
995 889 662 437 432 431 357 353
239 184 120
EcoO109I rG'GnC_Cy
Cuts at: 1554 2238 1554
Size: 684 4415
EcoRI G'AATT_C
Cuts at: 2103 2103
Size: 5099
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 1573 3118 372
Size: 1201 1545 2353
Fragments arranged by size:
2353 1545 1201
FspI TGC'GCA
Cuts at: 2880 2880
Size: 5099
HaeII r_GCGC'y
Cuts at: 219 894 2533 2781 4011 4782 219
Size: 675 1639 248 1230 771 536
Fragments arranged by size:
1639 1230 771 675 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 499 531 662 694 1269 1301 1375
Size: 32 310 32 131 32 575 32 74
Cuts at: 1375 1407 2941 2971 2973 3003 157
Size: 32 1534 30 2 30 2253
Fragments arranged by size:
2253 1534 575 310 131 74 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2079 2138 2079
Size: 59 5040
KasI G'GCGC_C
Cuts at: 890 2529 2777 890
Size: 1639 248 3212
Fragments arranged by size:
3212 1639 248
KpnI G_GTAC'C
Cuts at: 2119 2119
Size: 5099
MluI A'CGCG_T
Cuts at: 4532 4532
Size: 5099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1757 3858 4042 1145
Size: 612 2101 184 2202
Fragments arranged by size:
2202 2101 612 184
MscI TGG'CCA
Cuts at: 1988 2860 1988
Size: 872 4227
MslI CAynn'nnrTG
Cuts at: 1243 3088 3215 1243
Size: 1845 127 3127
Fragments arranged by size:
3127 1845 127
MspA1I CmG'CkG
Cuts at: 2210 2524 2884 3668 3913 2210
Size: 314 360 784 245 3396
Fragments arranged by size:
3396 784 360 314 245
NaeI GCC'GGC
Cuts at: 3280 3280
Size: 5099
NarI GG'CG_CC
Cuts at: 891 2530 2778 891
Size: 1639 248 3212
Fragments arranged by size:
3212 1639 248
NcoI C'CATG_G
Cuts at: 1521 1521
Size: 5099
NgoMIV G'CCGG_C
Cuts at: 3278 3278
Size: 5099
NspI r_CATG'y
Cuts at: 736 1511 1645 2152 2652 4255 736
Size: 775 134 507 500 1603 1580
Fragments arranged by size:
1603 1580 775 507 500 134
PasI CC'CwG_GG
Cuts at: 2070 2070
Size: 5099
PciI A'CATG_T
Cuts at: 1641 4251 1641
Size: 2610 2489
PfoI T'CCnGG_A
Cuts at: 1077 1077
Size: 5099
PmeI GTTT'AAAC
Cuts at: 2098 2098
Size: 5099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 539 571 3545 3577 539
Size: 32 2974 32 2061
Fragments arranged by size:
2974 2061 32 32
PsiI TTA'TAA
Cuts at: 2063 2063
Size: 5099
PstI C_TGCA'G
Cuts at: 804 2146 2831 804
Size: 1342 685 3072
Fragments arranged by size:
3072 1342 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5099
PvuII CAG'CTG
Cuts at: 2524 2884 2524
Size: 360 4739
RsrII CG'GwC_CG
Cuts at: 3294 3294
Size: 5099
SacI G_AGCT'C
Cuts at: 2113 2113
Size: 5099
SalI G'TCGA_C
Cuts at: 2136 2136
Size: 5099
SapI GCTCTTCn'nnn_
Cuts at: 3122 3332 3122
Size: 210 4889
SbfI CC_TGCA'GG
Cuts at: 2146 2146
Size: 5099
ScaI AGT'ACT
Cuts at: 4616 4616
Size: 5099
SfcI C'TryA_G
Cuts at: 33 800 1319 1598 2142 2827 3795 3986 33
Size: 767 519 279 544 685 968 191 1146
Fragments arranged by size:
1146 968 767 685 544 519 279 191
SfoI GGC'GCC
Cuts at: 892 2531 2779 892
Size: 1639 248 3212
Fragments arranged by size:
3212 1639 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5099
SmaI CCC'GGG
Cuts at: 2121 2121
Size: 5099
SmlI C'TyrA_G
Cuts at: 822 2254 3169 3883 4145 822
Size: 1432 915 714 262 1776
Fragments arranged by size:
1776 1432 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5099
SphI G_CATG'C
Cuts at: 736 2152 2652 736
Size: 1416 500 3183
Fragments arranged by size:
3183 1416 500
SspI AAT'ATT
Cuts at: 1761 1761
Size: 5099
StyI C'CwwG_G
Cuts at: 914 1521 1719 2233 2587 914
Size: 607 198 514 354 3426
Fragments arranged by size:
3426 607 514 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 2682 2815 3274 3483 1359
Size: 1323 133 459 209 2975
Fragments arranged by size:
2975 1323 459 209 133
TatI w'GTAC_w
Cuts at: 199 945 1683 4614 199
Size: 746 738 2931 684
Fragments arranged by size:
2931 746 738 684
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1073 1105 4616 4648 1073
Size: 32 3511 32 1524
Fragments arranged by size:
3511 1524 32 32
Tth111I GACn'n_nGTC
Cuts at: 2896 2896
Size: 5099
XbaI T'CTAG_A
Cuts at: 985 2130 985
Size: 1145 3954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 154 787 2594 114
Size: 40 633 1807 2619
Fragments arranged by size:
2619 1807 633 40
XmaI C'CCGG_G
Cuts at: 2119 2119
Size: 5099
XmnI GAAnn'nnTTC
Cuts at: 972 972
Size: 5099
ZraI GAC'GTC
Cuts at: 1084 1084
Size: 5099
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BmtI BsmI BsmBI
BspEI BssHII BstEII BstXI BstZ17I ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI