Restriction map for
pF1KB3433
(Circular) (Six-base) MAPSORT of: pF1KB3433.seq Check: 2141 from: 1 to: 4394
pF1KB3433 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 2219 2651 3004 3666 753
Size: 1466 432 353 662 1481
Fragments arranged by size:
1481 1466 662 432 353
AflII C'TTAA_G
Cuts at: 619 619
Size: 4394
AflIII A'CryG_T
Cuts at: 389 1746 3546 3827 389
Size: 1357 1800 281 956
Fragments arranged by size:
1800 1357 956 281
AlwNI CAG_nnn'CTG
Cuts at: 1174 1333 1699 3137 1174
Size: 159 366 1438 2431
Fragments arranged by size:
2431 1438 366 159
ApaI G_GGCC'C
Cuts at: 727 727
Size: 4394
ApaLI G'TGCA_C
Cuts at: 3232 3232
Size: 4394
ApoI r'AATT_y
Cuts at: 332 1398 3747 4003 332
Size: 1066 2349 256 723
Fragments arranged by size:
2349 1066 723 256
AseI AT'TA_AT
Cuts at: 20 962 20
Size: 942 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 431 1410 1824 2072 2107 431
Size: 979 414 248 35 2718
Fragments arranged by size:
2718 979 414 248 35
BanII G_rGCy'C
Cuts at: 727 1011 1408 2438 727
Size: 284 397 1030 2683
Fragments arranged by size:
2683 1030 397 284
BbeI G_GCGC'C
Cuts at: 435 1828 2076 435
Size: 1393 248 2753
Fragments arranged by size:
2753 1393 248
BbsI GAAGACnn'nnnn_
Cuts at: 601 601
Size: 4394
BbvCI CC'TCA_GC
Cuts at: 498 498
Size: 4394
BciVI GTATCCnnnnn_n'
Cuts at: 721 2292 3348 4394 721
Size: 1571 1056 1046 721
Fragments arranged by size:
1571 1056 1046 721
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglII A'GATC_T
Cuts at: 969 1909 3946 969
Size: 940 2037 1417
Fragments arranged by size:
2037 1417 940
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 727 2019 2112 3236 727
Size: 1292 93 1124 1885
Fragments arranged by size:
1885 1292 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
Bpu10I CC'TnA_GC
Cuts at: 498 498
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1092 1570 2449 3163 3461 910
Size: 182 478 879 714 298 1843
Fragments arranged by size:
1843 879 714 478 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 432 1825 2073 2775 4271 432
Size: 1393 248 702 1496 555
Fragments arranged by size:
1496 1393 702 555 248
BsaWI w'CCGG_w
Cuts at: 878 1583 2104 2782 3193 3340 3614 878
Size: 705 521 678 411 147 274 1658
Fragments arranged by size:
1658 705 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1384 1384
Size: 4394
BseYI C'CCAG_C
Cuts at: 1819 2577 3242 3650 1819
Size: 758 665 408 2563
Fragments arranged by size:
2563 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3212 104
Size: 1832 46 1230 1286
Fragments arranged by size:
1832 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 120 1020 1408 2186 2376 3236 120
Size: 900 388 778 190 860 1278
Fragments arranged by size:
1278 900 860 778 388 190
Bsp1286I G_dGCh'C
Cuts at: 120 727 1011 1020 1408 2019 2112 2186 2376
Size: 607 284 9 388 611 93 74 190
Cuts at: 2376 2438 3236 120
Size: 62 798 1278
Fragments arranged by size:
1278 798 611 607 388 284 190 93
74 62 9
BspEI T'CCGG_A
Cuts at: 878 878
Size: 4394
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 271 1444 1658 1960 2341 271
Size: 1173 214 302 381 2324
Fragments arranged by size:
2324 1173 381 302 214
BsrBI CCG'CTC
Cuts at: 50 365 2686 2740 4224 4389 50
Size: 315 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 315 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 2392 2573 2392
Size: 181 4213
BssSI C'ACGA_G
Cuts at: 2665 3373 2665
Size: 708 3686
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1771 1174
Size: 597 3797
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstYI r'GATC_y
Cuts at: 1 969 998 1031 1419 1886 1909 2244 2490
Size: 968 29 33 388 467 23 335 246
Cuts at: 2490 2796 2808 2894 3946 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 968 467 449 388 335 306 246
86 33 29 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1021 2344 2525 2688 1021
Size: 1323 181 163 2727
Fragments arranged by size:
2727 1323 181 163
BtsI GCAGTG_nn'
Cuts at: 1637 1637
Size: 4394
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 3444 2100
Size: 1344 3050
EaeI y'GGCC_r
Cuts at: 369 1979 2153 2544 2571 3916 369
Size: 1610 174 391 27 1345 847
Fragments arranged by size:
1610 1345 847 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 3344 3490 4204 2602
Size: 742 146 714 2792
Fragments arranged by size:
2792 742 714 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 753 2219 2651 3004 3666 753
Size: 1466 432 353 662 1481
Fragments arranged by size:
1481 1466 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1238 1238
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 723 1533 723
Size: 810 3584
EcoRI G'AATT_C
Cuts at: 332 1398 332
Size: 1066 3328
EcoRV GAT'ATC
Cuts at: 131 645 131
Size: 514 3880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 241 2175 241
Size: 1934 2460
HaeII r_GCGC'y
Cuts at: 435 1828 2076 3306 4077 435
Size: 1393 248 1230 771 752
Fragments arranged by size:
1393 1230 771 752 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 687 719 2236 2266 2268 2298 461
Size: 32 194 32 1517 30 2 30 2557
Fragments arranged by size:
2557 1517 194 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
HindIII A'AGCT_T
Cuts at: 823 823
Size: 4394
KasI G'GCGC_C
Cuts at: 431 1824 2072 431
Size: 1393 248 2753
Fragments arranged by size:
2753 1393 248
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3153 3337 3153
Size: 184 4210
MscI TGG'CCA
Cuts at: 371 2155 371
Size: 1784 2610
MslI CAynn'nnrTG
Cuts at: 2383 2510 2383
Size: 127 4267
MspA1I CmG'CkG
Cuts at: 1171 1183 1505 1819 2179 2963 3208 1171
Size: 12 322 314 360 784 245 2357
Fragments arranged by size:
2357 784 360 322 314 245 12
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 432 1825 2073 432
Size: 1393 248 2753
Fragments arranged by size:
2753 1393 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4394
NdeI CA'TA_TG
Cuts at: 1098 1098
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 393 844 1447 1947 3550 393
Size: 451 603 500 1603 1237
Fragments arranged by size:
1603 1237 603 500 451
PciI A'CATG_T
Cuts at: 389 3546 389
Size: 3157 1237
PflMI CCAn_nnn'nTGG
Cuts at: 428 428
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 4362
PspOMI G'GGCC_C
Cuts at: 723 723
Size: 4394
PstI C_TGCA'G
Cuts at: 1179 1350 1389 1441 2126 1179
Size: 171 39 52 685 3447
Fragments arranged by size:
3447 685 171 52 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1171 1819 2179 1171
Size: 648 360 3386
Fragments arranged by size:
3386 648 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1175 1346 1364 1385 1437 2122 3090 3281
Size: 1142 171 18 21 52 685 968 191
Cuts at: 3281 33
Size: 1146
Fragments arranged by size:
1146 1142 968 685 191 171 52 21
18
SfoI GGC'GCC
Cuts at: 433 1826 2074 433
Size: 1393 248 2753
Fragments arranged by size:
2753 1393 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 619 925 1071 1549 2464 3178 3440 619
Size: 306 146 478 915 714 262 1573
Fragments arranged by size:
1573 915 714 478 306 262 146
SpeI A'CTAG_T
Cuts at: 67 292 67
Size: 225 4169
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 766 766
Size: 4394
StyI C'CwwG_G
Cuts at: 107 599 1528 1882 107
Size: 492 929 354 2619
Fragments arranged by size:
2619 929 492 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2110 2569 2778 1977
Size: 133 459 209 3593
Fragments arranged by size:
3593 459 209 133
TatI w'GTAC_w
Cuts at: 137 3909 137
Size: 3772 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 855 887 3911 3943 855
Size: 32 3024 32 1306
Fragments arranged by size:
3024 1306 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 379 425 1889 379
Size: 46 1464 2884
Fragments arranged by size:
2884 1464 46
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI