Restriction map for
pF1KB3404
(Circular) (Six-base) MAPSORT of: pF1KB3404.seq Check: 3225 from: 1 to: 8186
pF1KB3404 8186 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 2490 5224 2490
Size: 2734 5452
Acc65I G'GTAC_C
Cuts at: 5202 5202
Size: 8186
AclI AA'CG_TT
Cuts at: 1723 8026 1723
Size: 6303 1883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 650 799 1451 2048 2125 2447 2605 2666
Size: 482 149 652 597 77 322 158 61
Cuts at: 2666 2933 3362 4071 4605 6011 6443 7190 7458
Size: 267 429 709 534 1406 432 747 268
Cuts at: 7458 168
Size: 896
Fragments arranged by size:
1406 896 747 709 652 597 534 482
432 429 322 268 267 158 149 77
61
AflII C'TTAA_G
Cuts at: 1956 2260 2484 1956
Size: 304 224 7658
Fragments arranged by size:
7658 304 224
AflIII A'CryG_T
Cuts at: 3741 5538 6642 7619 3741
Size: 1797 1104 977 4308
Fragments arranged by size:
4308 1797 1104 977
AgeI A'CCGG_T
Cuts at: 6574 6574
Size: 8186
AhdI GACnn_n'nnGTC
Cuts at: 903 3194 903
Size: 2291 5895
AlwNI CAG_nnn'CTG
Cuts at: 817 3538 4758 5491 7058 817
Size: 2721 1220 733 1567 1945
Fragments arranged by size:
2721 1945 1567 1220 733
ApaI G_GGCC'C
Cuts at: 161 161
Size: 8186
ApaLI G'TGCA_C
Cuts at: 6956 6956
Size: 8186
ApoI r'AATT_y
Cuts at: 610 1215 1245 1840 2374 2667 3403 3604 4039
Size: 605 30 595 534 293 736 201 435
Cuts at: 4039 4049 4930 5190 7539 7795 610
Size: 10 881 260 2349 256 1001
Fragments arranged by size:
2349 1001 881 736 605 595 534 435
293 260 256 201 30 10
AseI AT'TA_AT
Cuts at: 20 4507 4693 20
Size: 4487 186 3513
Fragments arranged by size:
4487 3513 186
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8186
AvaI C'yCGr_G
Cuts at: 5206 5206
Size: 8186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2599 2632 2599
Size: 33 8153
BamHI G'GATC_C
Cuts at: 5211 5211
Size: 8186
BanI G'GyrC_C
Cuts at: 5202 5616 5864 5899 5202
Size: 414 248 35 7489
Fragments arranged by size:
7489 414 248 35
BanII G_rGCy'C
Cuts at: 161 3963 4990 5200 6230 161
Size: 3802 1027 210 1030 2117
Fragments arranged by size:
3802 2117 1030 1027 210
BbeI G_GCGC'C
Cuts at: 5620 5868 5620
Size: 248 7938
BbsI GAAGACnn'nnnn_
Cuts at: 338 1118 338
Size: 780 7406
BciVI GTATCCnnnnn_n'
Cuts at: 6084 6845 8186 6084
Size: 761 1341 6084
Fragments arranged by size:
6084 1341 761
BclI T'GATC_A
Cuts at: 3813 5706 3813
Size: 1893 6293
BglI GCCn_nnn'nGGC
Cuts at: 2788 2788
Size: 8186
BglII A'GATC_T
Cuts at: 1743 2523 2923 4235 4303 4764 5701 7738 1743
Size: 780 400 1312 68 461 937 2037 2191
Fragments arranged by size:
2191 2037 1312 937 780 461 400 68
BlpI GC'TnA_GC
Cuts at: 5298 5298
Size: 8186
Bme1580I G_kGCm'C
Cuts at: 161 5811 5904 6960 161
Size: 5650 93 1056 1387
Fragments arranged by size:
5650 1387 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3356 5110 5398 5479 5814 7958 3356
Size: 1754 288 81 335 2144 3584
Fragments arranged by size:
3584 2144 1754 335 288 81
BmtI G_CTAG'C
Cuts at: 3532 4877 3532
Size: 1345 6841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6553 6553
Size: 8186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 812 2767 3290 4224 4760 4958 5170 134
Size: 678 1955 523 934 536 198 212 3150
Fragments arranged by size:
3150 1955 934 678 536 523 212 198
Bpu10I CC'TnA_GC
Cuts at: 1049 2628 3260 4850 5098 1049
Size: 1579 632 1590 248 4137
Fragments arranged by size:
4137 1590 1579 632 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 5362 6241 6733 7031 1480
Size: 3882 879 492 298 2635
Fragments arranged by size:
3882 2635 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3605 3883 4489 7890 3605
Size: 278 606 3401 3901
Fragments arranged by size:
3901 3401 606 278
BsaAI yAC'GTr
Cuts at: 2053 5539 2053
Size: 3486 4700
BsaBI GATnn'nnATC
Cuts at: 6 4081 5700 7737 6
Size: 4075 1619 2037 455
Fragments arranged by size:
4075 2037 1619 455
BsaHI Gr'CG_yC
Cuts at: 5617 5865 6567 8063 5617
Size: 248 702 1496 5740
Fragments arranged by size:
5740 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4999 5375 5896 6574 6848 6995 7406 4999
Size: 376 521 678 274 147 411 5779
Fragments arranged by size:
5779 678 521 411 376 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2740 2770 2740
Size: 30 8156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 377 4668 377
Size: 4291 3895
BseYI C'CCAG_C
Cuts at: 3488 5028 5171 5611 6369 6946 7442 3488
Size: 1540 143 440 758 577 496 4232
Fragments arranged by size:
4232 1540 758 577 496 440 143
BsiEI CG_ry'CG
Cuts at: 104 5728 5774 6982 104
Size: 5624 46 1208 1308
Fragments arranged by size:
5624 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2069 2945 3297 3963 5158 5200 5978 6168 6960
Size: 876 352 666 1195 42 778 190 792
Cuts at: 6960 2069
Size: 3295
Fragments arranged by size:
3295 1195 876 792 778 666 352 190
42
BsmI GAATG_Cn'
Cuts at: 235 2958 5070 235
Size: 2723 2112 3351
Fragments arranged by size:
3351 2723 2112
Bsp1286I G_dGCh'C
Cuts at: 161 2069 2945 3297 3963 4990 5158 5200 5811
Size: 1908 876 352 666 1027 168 42 611
Cuts at: 5811 5904 5978 6168 6230 6960 161
Size: 93 74 190 62 730 1387
Fragments arranged by size:
1908 1387 1027 876 730 666 611 352
190 168 93 74 62 42
BspEI T'CCGG_A
Cuts at: 4999 7406 4999
Size: 2407 5779
BspHI T'CATG_A
Cuts at: 989 3696 7362 989
Size: 2707 3666 1813
Fragments arranged by size:
3666 2707 1813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3984 5236 5450 5752 6133 3984
Size: 1252 214 302 381 6037
Fragments arranged by size:
6037 1252 381 302 214
BsrBI CCG'CTC
Cuts at: 50 163 1860 3617 4862 6478 6532 8016 8181
Size: 113 1697 1757 1245 1616 54 1484 165
Cuts at: 8181 50
Size: 55
Fragments arranged by size:
1757 1697 1616 1484 1245 165 113 55
54
BsrDI GCAATG_nn'
Cuts at: 6098 6098
Size: 8186
BsrFI r'CCGG_y
Cuts at: 6184 6365 6574 6184
Size: 181 209 7796
Fragments arranged by size:
7796 209 181
BsrGI T'GTAC_A
Cuts at: 699 3738 4376 699
Size: 3039 638 4509
Fragments arranged by size:
4509 3039 638
BssSI C'ACGA_G
Cuts at: 2609 2684 6457 6815 2609
Size: 75 3773 358 3980
Fragments arranged by size:
3980 3773 358 75
BstAPI GCAn_nnn'nTGC
Cuts at: 1968 4968 5563 1968
Size: 3000 595 4591
Fragments arranged by size:
4591 3000 595
BstBI TT'CG_AA
Cuts at: 1062 6547 1062
Size: 5485 2701
BstXI CCAn_nnnn'nTGG
Cuts at: 1380 1380
Size: 8186
BstYI r'GATC_y
Cuts at: 1 1053 1383 1743 2523 2923 3210 4235 4303
Size: 1052 330 360 780 400 287 1025 68
Cuts at: 4303 4671 4754 4764 5211 5678 5701 6036 6282
Size: 368 83 10 447 467 23 335 246
Cuts at: 6282 7294 7380 7392 7738 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1052 1025 1012 780 467 449 447 400
368 360 346 335 330 287 246 86
83 68 23 12 10
BstZ17I GTA'TAC
Cuts at: 2491 2491
Size: 8186
Bsu36I CC'TnA_GG
Cuts at: 4787 4787
Size: 8186
BtgI C'CryG_G
Cuts at: 860 3954 4415 5163 860
Size: 3094 461 748 3883
Fragments arranged by size:
3883 3094 748 461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2609 6136 6317 6480 2609
Size: 3527 181 163 4315
Fragments arranged by size:
4315 3527 181 163
BtsI GCAGTG_nn'
Cuts at: 184 4884 5429 184
Size: 4700 545 2941
Fragments arranged by size:
4700 2941 545
DraI TTT'AAA
Cuts at: 1241 2324 4044 5185 1241
Size: 1083 1720 1141 4242
Fragments arranged by size:
4242 1720 1141 1083
DrdI GACnn_nn'nnGTC
Cuts at: 1035 3619 3845 5892 6750 1035
Size: 2584 226 2047 858 2471
Fragments arranged by size:
2584 2471 2047 858 226
EaeI y'GGCC_r
Cuts at: 1287 1829 4327 5771 5945 6336 6363 7708 1287
Size: 542 2498 1444 174 391 27 1345 1765
Fragments arranged by size:
2498 1765 1444 1345 542 391 174 27
EagI C'GGCC_G
Cuts at: 5771 5771
Size: 8186
EarI CTCTTCn'nnn_
Cuts at: 246 1916 2343 2507 2795 2909 4439 4628 6209
Size: 1670 427 164 288 114 1530 189 1581
Cuts at: 6209 6419 246
Size: 210 2013
Fragments arranged by size:
2013 1670 1581 1530 427 288 210 189
164 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6394 6704 6850 7996 6394
Size: 310 146 1146 6584
Fragments arranged by size:
6584 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3961 5198 3961
Size: 1237 6949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 168 650 799 812 1451 2048 2125 2447
Size: 34 482 149 13 639 597 77 322
Cuts at: 2447 2605 2666 2767 2933 3290 3362 4071 4224
Size: 158 61 101 166 357 72 709 153
Cuts at: 4224 4605 4760 4958 5170 6011 6443 7190 7458
Size: 381 155 198 212 841 432 747 268
Cuts at: 7458 134
Size: 862
Fragments arranged by size:
862 841 747 709 639 597 482 432
381 357 322 268 212 198 166 158
155 153 149 101 77 72 61 34
13
EcoNI CCTnn'n_nnAGG
Cuts at: 3218 4853 3218
Size: 1635 6551
EcoO109I rG'GnC_Cy
Cuts at: 3196 3996 4735 5175 5325 3196
Size: 800 739 440 150 6057
Fragments arranged by size:
6057 800 739 440 150
EcoRI G'AATT_C
Cuts at: 1840 4930 5190 1840
Size: 3090 260 4836
Fragments arranged by size:
4836 3090 260
EcoRV GAT'ATC
Cuts at: 4398 4398
Size: 8186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1489 2886 3725 6205 1489
Size: 1397 839 2480 3470
Fragments arranged by size:
3470 2480 1397 839
FspI TGC'GCA
Cuts at: 1972 4227 5967 1972
Size: 2255 1740 4191
Fragments arranged by size:
4191 2255 1740
HaeII r_GCGC'y
Cuts at: 1458 5620 5868 6890 7869 1458
Size: 4162 248 1022 979 1775
Fragments arranged by size:
4162 1775 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 823 855 889 921 2740 2772 2908 2940 3180
Size: 32 34 32 1819 32 136 32 240
Cuts at: 3180 3212 4396 4416 4422 4428 4448 4454 5044
Size: 32 1184 20 6 6 20 6 590
Cuts at: 5044 5076 6028 6058 6060 6090 823
Size: 32 952 30 2 30 2919
Fragments arranged by size:
2919 1819 1184 952 590 240 136 34
32 32 32 32 32 32 30 30
20 20 6 6 6 2
HincII GTy'rAC
Cuts at: 1502 2423 5225 1502
Size: 921 2802 4463
Fragments arranged by size:
4463 2802 921
HindIII A'AGCT_T
Cuts at: 1433 1953 2461 1433
Size: 520 508 7158
Fragments arranged by size:
7158 520 508
HpaI GTT'AAC
Cuts at: 2423 2423
Size: 8186
KasI G'GCGC_C
Cuts at: 5616 5864 5616
Size: 248 7938
KpnI G_GTAC'C
Cuts at: 5206 5206
Size: 8186
MfeI C'AATT_G
Cuts at: 671 671
Size: 8186
MluI A'CGCG_T
Cuts at: 7619 7619
Size: 8186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 3184 3430 4472 4555 4729 4777 5108 5117
Size: 3068 246 1042 83 174 48 331 9
Cuts at: 5117 6857 7041 116
Size: 1740 184 1261
Fragments arranged by size:
3068 1740 1261 1042 331 246 184 174
83 48 9
MscI TGG'CCA
Cuts at: 1289 1831 4329 5947 1289
Size: 542 2498 1618 3528
Fragments arranged by size:
3528 2498 1618 542
MslI CAynn'nnrTG
Cuts at: 2243 3972 4175 6175 6302 2243
Size: 1729 203 2000 127 4127
Fragments arranged by size:
4127 2000 1729 203 127
MspA1I CmG'CkG
Cuts at: 2898 3268 5165 5297 5611 5971 6984 7229 2898
Size: 370 1897 132 314 360 1013 245 3855
Fragments arranged by size:
3855 1897 1013 370 360 314 245 132
NaeI GCC'GGC
Cuts at: 6367 6367
Size: 8186
NarI GG'CG_CC
Cuts at: 5617 5865 5617
Size: 248 7938
NcoI C'CATG_G
Cuts at: 860 3954 4415 860
Size: 3094 461 4631
Fragments arranged by size:
4631 3094 461
NdeI CA'TA_TG
Cuts at: 3237 3237
Size: 8186
NgoMIV G'CCGG_C
Cuts at: 6365 6365
Size: 8186
NheI G'CTAG_C
Cuts at: 3528 4873 3528
Size: 1345 6841
NspI r_CATG'y
Cuts at: 470 2242 3745 3971 5239 5739 6646 470
Size: 1772 1503 226 1268 500 907 2010
Fragments arranged by size:
2010 1772 1503 1268 907 500 226
PasI CC'CwG_GG
Cuts at: 3201 3201
Size: 8186
PciI A'CATG_T
Cuts at: 3741 6642 3741
Size: 2901 5285
PflMI CCAn_nnn'nTGG
Cuts at: 1306 4077 4421 1306
Size: 2771 344 5071
Fragments arranged by size:
5071 2771 344
PfoI T'CCnGG_A
Cuts at: 539 4737 539
Size: 4198 3988
PmeI GTTT'AAAC
Cuts at: 5185 5185
Size: 8186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7325 7357 7325
Size: 32 8154
PpuMI rG'GwC_Cy
Cuts at: 3196 3996 4735 5175 3196
Size: 800 739 440 6207
Fragments arranged by size:
6207 800 739 440
PspOMI G'GGCC_C
Cuts at: 157 157
Size: 8186
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4535 4567 4535
Size: 32 8154
PstI C_TGCA'G
Cuts at: 1526 5233 5918 1526
Size: 3707 685 3794
Fragments arranged by size:
3794 3707 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8186
PvuII CAG'CTG
Cuts at: 2898 3268 5611 5971 2898
Size: 370 2343 360 5113
Fragments arranged by size:
5113 2343 370 360
RsrII CG'GwC_CG
Cuts at: 6381 6381
Size: 8186
SacI G_AGCT'C
Cuts at: 3963 5200 3963
Size: 1237 6949
SacII CC_GC'GG
Cuts at: 5166 5166
Size: 8186
SalI G'TCGA_C
Cuts at: 5223 5223
Size: 8186
SanDI GG'GwC_CC
Cuts at: 5175 5175
Size: 8186
SapI GCTCTTCn'nnn_
Cuts at: 2909 6209 6419 2909
Size: 3300 210 4676
Fragments arranged by size:
4676 3300 210
SbfI CC_TGCA'GG
Cuts at: 5233 5233
Size: 8186
ScaI AGT'ACT
Cuts at: 7703 7703
Size: 8186
SexAI A'CCwGG_T
Cuts at: 560 1931 560
Size: 1371 6815
SfcI C'TryA_G
Cuts at: 33 383 818 1522 3302 4947 5229 5914 6907
Size: 350 435 704 1780 1645 282 685 993
Cuts at: 6907 7098 33
Size: 191 1121
Fragments arranged by size:
1780 1645 1121 993 704 685 435 350
282 191
SfoI GGC'GCC
Cuts at: 5618 5866 5618
Size: 248 7938
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8186
SmaI CCC'GGG
Cuts at: 5208 5208
Size: 8186
SmlI C'TyrA_G
Cuts at: 1459 1956 2260 2484 5341 6256 6748 7010 1459
Size: 497 304 224 2857 915 492 262 2635
Fragments arranged by size:
2857 2635 915 497 492 304 262 224
SpeI A'CTAG_T
Cuts at: 67 1321 1758 67
Size: 1254 437 6495
Fragments arranged by size:
6495 1254 437
SphI G_CATG'C
Cuts at: 5239 5739 5239
Size: 500 7686
SspI AAT'ATT
Cuts at: 344 428 1158 344
Size: 84 730 7372
Fragments arranged by size:
7372 730 84
StuI AGG'CCT
Cuts at: 2626 2626
Size: 8186
StyI C'CwwG_G
Cuts at: 860 3954 4415 5013 5320 5674 860
Size: 3094 461 598 307 354 3372
Fragments arranged by size:
3372 3094 598 461 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1142 1894 3549 3907 5769 5902 6361 6570 1142
Size: 752 1655 358 1862 133 459 209 2758
Fragments arranged by size:
2758 1862 1655 752 459 358 209 133
TatI w'GTAC_w
Cuts at: 699 1332 3738 4376 7701 699
Size: 633 2406 638 3325 1184
Fragments arranged by size:
3325 2406 1184 638 633
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1496 1528 2037 2069 4309 4341 7703 7735 1496
Size: 32 509 32 2240 32 3362 32 1947
Fragments arranged by size:
3362 2240 1947 509 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1094 5983 1094
Size: 4889 3297
XbaI T'CTAG_A
Cuts at: 1410 4306 5217 1410
Size: 2896 911 4379
Fragments arranged by size:
4379 2896 911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1995 5681 1995
Size: 3686 4500
XmaI C'CCGG_G
Cuts at: 5206 5206
Size: 8186
XmnI GAAnn'nnTTC
Cuts at: 851 2579 3803 4013 4424 851
Size: 1728 1224 210 411 4613
Fragments arranged by size:
4613 1728 1224 411 210
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BsgI BsiWI BsmBI BssHII
BstEII ClaI CspCI DraIII FseI FspAI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI