Restriction map for
pF1KB3399
(Circular) (Six-base) MAPSORT of: pF1KB3399.seq Check: 4876 from: 1 to: 3671
pF1KB3399 3671 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 709 709
Size: 3671
Acc65I G'GTAC_C
Cuts at: 687 687
Size: 3671
AclI AA'CG_TT
Cuts at: 419 3511 419
Size: 3092 579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1496 1928 2675 2943 1496
Size: 432 747 268 2224
Fragments arranged by size:
2224 747 432 268
AflII C'TTAA_G
Cuts at: 189 189
Size: 3671
AflIII A'CryG_T
Cuts at: 1023 2127 3104 1023
Size: 1104 977 1590
Fragments arranged by size:
1590 1104 977
AgeI A'CCGG_T
Cuts at: 2059 2059
Size: 3671
AlwNI CAG_nnn'CTG
Cuts at: 976 2543 976
Size: 1567 2104
ApaI G_GGCC'C
Cuts at: 593 593
Size: 3671
ApaLI G'TGCA_C
Cuts at: 2441 2441
Size: 3671
ApoI r'AATT_y
Cuts at: 340 463 659 675 3024 3280 340
Size: 123 196 16 2349 256 731
Fragments arranged by size:
2349 731 256 196 123 16
AseI AT'TA_AT
Cuts at: 20 250 20
Size: 230 3441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
AvaI C'yCGr_G
Cuts at: 691 691
Size: 3671
BamHI G'GATC_C
Cuts at: 696 696
Size: 3671
BanI G'GyrC_C
Cuts at: 346 687 1101 1349 1384 346
Size: 341 414 248 35 2633
Fragments arranged by size:
2633 414 341 248 35
BanII G_rGCy'C
Cuts at: 593 685 1715 593
Size: 92 1030 2549
Fragments arranged by size:
2549 1030 92
BbeI G_GCGC'C
Cuts at: 1105 1353 1105
Size: 248 3423
BciVI GTATCCnnnnn_n'
Cuts at: 1569 2330 3671 1569
Size: 761 1341 1569
Fragments arranged by size:
1569 1341 761
BclI T'GATC_A
Cuts at: 1191 1191
Size: 3671
BglII A'GATC_T
Cuts at: 1186 3223 1186
Size: 2037 1634
BlpI GC'TnA_GC
Cuts at: 357 783 357
Size: 426 3245
Bme1580I G_kGCm'C
Cuts at: 593 1296 1389 2445 593
Size: 703 93 1056 1819
Fragments arranged by size:
1819 1056 703 93
BmrI ACTGGGnnnn_n'
Cuts at: 883 964 1299 3443 883
Size: 81 335 2144 1111
Fragments arranged by size:
2144 1111 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 3671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1726 2218 2516 847
Size: 879 492 298 2002
Fragments arranged by size:
2002 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3375 3375
Size: 3671
BsaAI yAC'GTr
Cuts at: 1024 1024
Size: 3671
BsaBI GATnn'nnATC
Cuts at: 6 1185 3222 6
Size: 1179 2037 455
Fragments arranged by size:
2037 1179 455
BsaHI Gr'CG_yC
Cuts at: 1102 1350 2052 3548 1102
Size: 248 702 1496 1225
Fragments arranged by size:
1496 1225 702 248
BsaWI w'CCGG_w
Cuts at: 860 1381 2059 2333 2480 2891 860
Size: 521 678 274 147 411 1640
Fragments arranged by size:
1640 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 203
Size: 30 3641
BseYI C'CCAG_C
Cuts at: 1096 1854 2431 2927 1096
Size: 758 577 496 1840
Fragments arranged by size:
1840 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1213 1259 2467 104
Size: 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 46
BsiHKAI G_wGCw'C
Cuts at: 685 1463 1653 2445 685
Size: 778 190 792 1911
Fragments arranged by size:
1911 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 593 685 1296 1389 1463 1653 1715 2445 593
Size: 92 611 93 74 190 62 730 1819
Fragments arranged by size:
1819 730 611 190 93 92 74 62
BspEI T'CCGG_A
Cuts at: 2891 2891
Size: 3671
BspHI T'CATG_A
Cuts at: 2847 2847
Size: 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 721 935 1237 1618 721
Size: 214 302 381 2774
Fragments arranged by size:
2774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1963 2017 3501 3666 50
Size: 1913 54 1484 165 55
Fragments arranged by size:
1913 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1583 1583
Size: 3671
BsrFI r'CCGG_y
Cuts at: 1669 1850 2059 1669
Size: 181 209 3281
Fragments arranged by size:
3281 209 181
BssSI C'ACGA_G
Cuts at: 1942 2300 1942
Size: 358 3313
BstAPI GCAn_nnn'nTGC
Cuts at: 1048 1048
Size: 3671
BstBI TT'CG_AA
Cuts at: 2032 2032
Size: 3671
BstYI r'GATC_y
Cuts at: 1 696 1163 1186 1521 1767 2779 2865 2877
Size: 695 467 23 335 246 1012 86 12
Cuts at: 2877 3223 1
Size: 346 449
Fragments arranged by size:
1012 695 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 294 294
Size: 3671
BtgI C'CryG_G
Cuts at: 107 107
Size: 3671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 1802 1965 1621
Size: 181 163 3327
Fragments arranged by size:
3327 181 163
BtsI GCAGTG_nn'
Cuts at: 434 914 434
Size: 480 3191
DraI TTT'AAA
Cuts at: 185 670 185
Size: 485 3186
DrdI GACnn_nn'nnGTC
Cuts at: 1377 2235 1377
Size: 858 2813
EaeI y'GGCC_r
Cuts at: 1256 1430 1821 1848 3193 1256
Size: 174 391 27 1345 1734
Fragments arranged by size:
1734 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1256 1256
Size: 3671
EarI CTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1879 2189 2335 3481 1879
Size: 310 146 1146 2069
Fragments arranged by size:
2069 1146 310 146
EcoICRI GAG'CTC
Cuts at: 683 683
Size: 3671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1496 1928 2675 2943 335
Size: 1161 432 747 268 1063
Fragments arranged by size:
1161 1063 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 589 810 589
Size: 221 3450
EcoRI G'AATT_C
Cuts at: 463 675 463
Size: 212 3459
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1690 1690
Size: 3671
FspI TGC'GCA
Cuts at: 1452 1452
Size: 3671
HaeII r_GCGC'y
Cuts at: 1105 1353 2375 3354 1105
Size: 248 1022 979 1422
Fragments arranged by size:
1422 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 522 554 1513 1543 1545 1575 522
Size: 32 959 30 2 30 2618
Fragments arranged by size:
2618 959 32 30 30 2
HincII GTy'rAC
Cuts at: 417 710 417
Size: 293 3378
HindIII A'AGCT_T
Cuts at: 256 256
Size: 3671
HpaI GTT'AAC
Cuts at: 417 417
Size: 3671
KasI G'GCGC_C
Cuts at: 1101 1349 1101
Size: 248 3423
KpnI G_GTAC'C
Cuts at: 691 691
Size: 3671
MluI A'CGCG_T
Cuts at: 3104 3104
Size: 3671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 2342 2526 219
Size: 2123 184 1364
Fragments arranged by size:
2123 1364 184
MscI TGG'CCA
Cuts at: 1432 1432
Size: 3671
MslI CAynn'nnrTG
Cuts at: 1660 1787 1660
Size: 127 3544
MspA1I CmG'CkG
Cuts at: 782 1096 1456 2469 2714 782
Size: 314 360 1013 245 1739
Fragments arranged by size:
1739 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1852 1852
Size: 3671
NarI GG'CG_CC
Cuts at: 1102 1350 1102
Size: 248 3423
NcoI C'CATG_G
Cuts at: 107 107
Size: 3671
NgoMIV G'CCGG_C
Cuts at: 1850 1850
Size: 3671
NspI r_CATG'y
Cuts at: 441 724 1224 2131 441
Size: 283 500 907 1981
Fragments arranged by size:
1981 907 500 283
PciI A'CATG_T
Cuts at: 2127 2127
Size: 3671
PmeI GTTT'AAAC
Cuts at: 670 670
Size: 3671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 3639
PsiI TTA'TAA
Cuts at: 227 227
Size: 3671
PspOMI G'GGCC_C
Cuts at: 589 589
Size: 3671
PstI C_TGCA'G
Cuts at: 374 718 1403 374
Size: 344 685 2642
Fragments arranged by size:
2642 685 344
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3671
PvuII CAG'CTG
Cuts at: 1096 1456 1096
Size: 360 3311
RsrII CG'GwC_CG
Cuts at: 1866 1866
Size: 3671
SacI G_AGCT'C
Cuts at: 685 685
Size: 3671
SalI G'TCGA_C
Cuts at: 708 708
Size: 3671
SapI GCTCTTCn'nnn_
Cuts at: 1694 1904 1694
Size: 210 3461
SbfI CC_TGCA'GG
Cuts at: 718 718
Size: 3671
ScaI AGT'ACT
Cuts at: 3188 3188
Size: 3671
SfcI C'TryA_G
Cuts at: 33 370 714 1399 2392 2583 33
Size: 337 344 685 993 191 1121
Fragments arranged by size:
1121 993 685 344 337 191
SfoI GGC'GCC
Cuts at: 1103 1351 1103
Size: 248 3423
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3671
SmaI CCC'GGG
Cuts at: 693 693
Size: 3671
SmlI C'TyrA_G
Cuts at: 189 826 1741 2233 2495 189
Size: 637 915 492 262 1365
Fragments arranged by size:
1365 915 637 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3671
SphI G_CATG'C
Cuts at: 724 1224 724
Size: 500 3171
SspI AAT'ATT
Cuts at: 395 395
Size: 3671
StyI C'CwwG_G
Cuts at: 107 805 1159 107
Size: 698 354 2619
Fragments arranged by size:
2619 698 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1254 1387 1846 2055 1254
Size: 133 459 209 2870
Fragments arranged by size:
2870 459 209 133
TatI w'GTAC_w
Cuts at: 3186 3186
Size: 3671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 3639
Tth111I GACn'n_nGTC
Cuts at: 1468 1468
Size: 3671
XbaI T'CTAG_A
Cuts at: 702 702
Size: 3671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1166 1166
Size: 3671
XmaI C'CCGG_G
Cuts at: 691 691
Size: 3671
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI