Restriction map for
pF1KB3382
(Circular) (Six-base) MAPSORT of: pF1KB3382.seq Check: 3502 from: 1 to: 4376
pF1KB3382 4376 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 597 597
Size: 4376
AccI GT'mk_AC
Cuts at: 1414 1414
Size: 4376
Acc65I G'GTAC_C
Cuts at: 1392 1392
Size: 4376
AclI AA'CG_TT
Cuts at: 4216 4216
Size: 4376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2633 3380 3648 2201
Size: 432 747 268 2929
Fragments arranged by size:
2929 747 432 268
AflIII A'CryG_T
Cuts at: 887 1728 2832 3809 887
Size: 841 1104 977 1454
Fragments arranged by size:
1454 1104 977 841
AgeI A'CCGG_T
Cuts at: 2764 2764
Size: 4376
AhdI GACnn_n'nnGTC
Cuts at: 778 778
Size: 4376
AleI CACnn'nnGTG
Cuts at: 418 418
Size: 4376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 952 984 952
Size: 32 4344
AlwNI CAG_nnn'CTG
Cuts at: 1681 3248 1681
Size: 1567 2809
ApaLI G'TGCA_C
Cuts at: 3146 3146
Size: 4376
ApoI r'AATT_y
Cuts at: 978 1380 3729 3985 978
Size: 402 2349 256 1369
Fragments arranged by size:
2349 1369 402 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4376
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
AvaI C'yCGr_G
Cuts at: 1396 1396
Size: 4376
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 463 496 463
Size: 33 4343
BamHI G'GATC_C
Cuts at: 1401 1401
Size: 4376
BanI G'GyrC_C
Cuts at: 397 597 850 1222 1392 1806 2054 2089 397
Size: 200 253 372 170 414 248 35 2684
Fragments arranged by size:
2684 414 372 253 248 200 170 35
BanII G_rGCy'C
Cuts at: 430 541 581 1270 1390 2420 430
Size: 111 40 689 120 1030 2386
Fragments arranged by size:
2386 1030 689 120 111 40
BbeI G_GCGC'C
Cuts at: 1810 2058 1810
Size: 248 4128
BbsI GAAGACnn'nnnn_
Cuts at: 519 519
Size: 4376
BbvCI CC'TCA_GC
Cuts at: 323 1103 323
Size: 780 3596
BciVI GTATCCnnnnn_n'
Cuts at: 306 2274 3035 4376 306
Size: 1968 761 1341 306
Fragments arranged by size:
1968 1341 761 306
BclI T'GATC_A
Cuts at: 1896 1896
Size: 4376
BglI GCCn_nnn'nGGC
Cuts at: 719 761 719
Size: 42 4334
BglII A'GATC_T
Cuts at: 1891 3928 1891
Size: 2037 2339
BlpI GC'TnA_GC
Cuts at: 1488 1488
Size: 4376
Bme1580I G_kGCm'C
Cuts at: 402 937 1225 1243 2001 2094 3150 402
Size: 535 288 18 758 93 1056 1628
Fragments arranged by size:
1628 1056 758 535 288 93 18
BmgBI CAC'GTC
Cuts at: 240 240
Size: 4376
BmrI ACTGGGnnnn_n'
Cuts at: 1588 1669 2004 4148 1588
Size: 81 335 2144 1816
Fragments arranged by size:
2144 1816 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1116 1284 1315 1116
Size: 168 31 4177
Fragments arranged by size:
4177 168 31
Bpu10I CC'TnA_GC
Cuts at: 323 1103 323
Size: 780 3596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 2431 2923 3221 1552
Size: 879 492 298 2707
Fragments arranged by size:
2707 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 186 4080 186
Size: 3894 482
BsaAI yAC'GTr
Cuts at: 825 888 1729 825
Size: 63 841 3472
Fragments arranged by size:
3472 841 63
BsaBI GATnn'nnATC
Cuts at: 6 1890 3927 6
Size: 1884 2037 455
Fragments arranged by size:
2037 1884 455
BsaHI Gr'CG_yC
Cuts at: 1259 1807 2055 2757 4253 1259
Size: 548 248 702 1496 1382
Fragments arranged by size:
1496 1382 702 548 248
BsaWI w'CCGG_w
Cuts at: 303 1565 2086 2764 3038 3185 3596 303
Size: 1262 521 678 274 147 411 1083
Fragments arranged by size:
1262 1083 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1120 1140 1120
Size: 20 4356
BseYI C'CCAG_C
Cuts at: 144 249 255 328 1083 1236 1801 2559 3136
Size: 105 6 73 755 153 565 758 577
Cuts at: 3136 3632 144
Size: 496 888
Fragments arranged by size:
888 758 755 577 565 496 153 105
73 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 625 631 337
Size: 288 6 4082
Fragments arranged by size:
4082 288 6
BsiEI CG_ry'CG
Cuts at: 104 1918 1964 3172 104
Size: 1814 46 1208 1308
Fragments arranged by size:
1814 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1390 2168 2358 3150 1390
Size: 778 190 792 2616
Fragments arranged by size:
2616 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 402 430 541 581 937 1225 1243 1270 1390
Size: 28 111 40 356 288 18 27 120
Cuts at: 1390 2001 2094 2168 2358 2420 3150 402
Size: 611 93 74 190 62 730 1628
Fragments arranged by size:
1628 730 611 356 288 190 120 111
93 74 62 40 28 27 18
BspEI T'CCGG_A
Cuts at: 303 3596 303
Size: 3293 1083
BspHI T'CATG_A
Cuts at: 3552 3552
Size: 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 597 1426 1640 1942 2323 597
Size: 829 214 302 381 2650
Fragments arranged by size:
2650 829 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2668 2722 4206 4371 50
Size: 2618 54 1484 165 55
Fragments arranged by size:
2618 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 2288 393
Size: 1895 2481
BsrFI r'CCGG_y
Cuts at: 285 755 2374 2555 2764 285
Size: 470 1619 181 209 1897
Fragments arranged by size:
1897 1619 470 209 181
BssSI C'ACGA_G
Cuts at: 2647 3005 2647
Size: 358 4018
BstAPI GCAn_nnn'nTGC
Cuts at: 1753 1753
Size: 4376
BstBI TT'CG_AA
Cuts at: 2737 2737
Size: 4376
BstEII G'GTnAC_C
Cuts at: 679 679
Size: 4376
BstXI CCAn_nnnn'nTGG
Cuts at: 922 922
Size: 4376
BstYI r'GATC_y
Cuts at: 1 1401 1868 1891 2226 2472 3484 3570 3582
Size: 1400 467 23 335 246 1012 86 12
Cuts at: 3582 3928 1
Size: 346 449
Fragments arranged by size:
1400 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 642 642
Size: 4376
BtgI C'CryG_G
Cuts at: 747 747
Size: 4376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2326 2507 2670 2326
Size: 181 163 4032
Fragments arranged by size:
4032 181 163
BtsI GCAGTG_nn'
Cuts at: 1619 1619
Size: 4376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 732 767 732
Size: 35 4341
DraI TTT'AAA
Cuts at: 1375 1375
Size: 4376
DraIII CAC_nnn'GTG
Cuts at: 345 347 660 345
Size: 2 313 4061
Fragments arranged by size:
4061 313 2
DrdI GACnn_nn'nnGTC
Cuts at: 2082 2940 2082
Size: 858 3518
EaeI y'GGCC_r
Cuts at: 363 519 753 1011 1961 2135 2526 2553 3898
Size: 156 234 258 950 174 391 27 1345
Cuts at: 3898 363
Size: 841
Fragments arranged by size:
1345 950 841 391 258 234 174 156
27
EagI C'GGCC_G
Cuts at: 1961 1961
Size: 4376
EarI CTCTTCn'nnn_
Cuts at: 583 1105 2399 2609 583
Size: 522 1294 210 2350
Fragments arranged by size:
2350 1294 522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2584 2894 3040 4186 2584
Size: 310 146 1146 2774
Fragments arranged by size:
2774 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1388 1388
Size: 4376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1116 1284 1315 2201 2633 3380 3648 1116
Size: 168 31 886 432 747 268 1844
Fragments arranged by size:
1844 886 747 432 268 168 31
EcoNI CCTnn'n_nnAGG
Cuts at: 1093 1093
Size: 4376
EcoO109I rG'GnC_Cy
Cuts at: 137 191 1166 1515 137
Size: 54 975 349 2998
Fragments arranged by size:
2998 975 349 54
EcoRI G'AATT_C
Cuts at: 978 1380 978
Size: 402 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4376
FspI TGC'GCA
Cuts at: 2157 2157
Size: 4376
HaeII r_GCGC'y
Cuts at: 1810 2058 3080 4059 1810
Size: 248 1022 979 2127
Fragments arranged by size:
2127 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 764 796 2218 2248 2250 2280 764
Size: 32 1422 30 2 30 2860
Fragments arranged by size:
2860 1422 32 30 30 2
HincII GTy'rAC
Cuts at: 1415 1415
Size: 4376
KasI G'GCGC_C
Cuts at: 1806 2054 1806
Size: 248 4128
KpnI G_GTAC'C
Cuts at: 1396 1396
Size: 4376
MluI A'CGCG_T
Cuts at: 3809 3809
Size: 4376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 932 3047 3231 554
Size: 378 2115 184 1699
Fragments arranged by size:
2115 1699 378 184
MscI TGG'CCA
Cuts at: 365 1013 2137 365
Size: 648 1124 2604
Fragments arranged by size:
2604 1124 648
MslI CAynn'nnrTG
Cuts at: 418 2365 2492 418
Size: 1947 127 2302
Fragments arranged by size:
2302 1947 127
MspA1I CmG'CkG
Cuts at: 249 615 673 1236 1293 1325 1487 1801 2161
Size: 366 58 563 57 32 162 314 360
Cuts at: 2161 3174 3419 249
Size: 1013 245 1206
Fragments arranged by size:
1206 1013 563 366 360 314 245 162
58 57 32
NaeI GCC'GGC
Cuts at: 287 757 2557 287
Size: 470 1800 2106
Fragments arranged by size:
2106 1800 470
NarI GG'CG_CC
Cuts at: 1807 2055 1807
Size: 248 4128
NgoMIV G'CCGG_C
Cuts at: 285 755 2555 285
Size: 470 1800 2106
Fragments arranged by size:
2106 1800 470
NspI r_CATG'y
Cuts at: 339 1429 1929 2836 339
Size: 1090 500 907 1879
Fragments arranged by size:
1879 1090 907 500
PasI CC'CwG_GG
Cuts at: 402 442 402
Size: 40 4336
PciI A'CATG_T
Cuts at: 2832 2832
Size: 4376
PflMI CCAn_nnn'nTGG
Cuts at: 992 992
Size: 4376
PfoI T'CCnGG_A
Cuts at: 545 833 545
Size: 288 4088
PmeI GTTT'AAAC
Cuts at: 1375 1375
Size: 4376
PmlI CAC'GTG
Cuts at: 825 888 825
Size: 63 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 4344
PpuMI rG'GwC_Cy
Cuts at: 137 191 137
Size: 54 4322
PstI C_TGCA'G
Cuts at: 1423 2108 1423
Size: 685 3691
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4376
PvuII CAG'CTG
Cuts at: 249 615 673 1236 1293 1801 2161 249
Size: 366 58 563 57 508 360 2464
Fragments arranged by size:
2464 563 508 366 360 58 57
RsrII CG'GwC_CG
Cuts at: 2571 2571
Size: 4376
SacI G_AGCT'C
Cuts at: 1390 1390
Size: 4376
SalI G'TCGA_C
Cuts at: 1413 1413
Size: 4376
SapI GCTCTTCn'nnn_
Cuts at: 583 1105 2399 2609 583
Size: 522 1294 210 2350
Fragments arranged by size:
2350 1294 522 210
SbfI CC_TGCA'GG
Cuts at: 1423 1423
Size: 4376
ScaI AGT'ACT
Cuts at: 3893 3893
Size: 4376
SfcI C'TryA_G
Cuts at: 33 1419 2104 3097 3288 33
Size: 1386 685 993 191 1121
Fragments arranged by size:
1386 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 719 761 719
Size: 42 4334
SfoI GGC'GCC
Cuts at: 1808 2056 1808
Size: 248 4128
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
SmaI CCC'GGG
Cuts at: 1398 1398
Size: 4376
SmlI C'TyrA_G
Cuts at: 1531 2446 2938 3200 1531
Size: 915 492 262 2707
Fragments arranged by size:
2707 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4376
SphI G_CATG'C
Cuts at: 339 1429 1929 339
Size: 1090 500 2786
Fragments arranged by size:
2786 1090 500
StuI AGG'CCT
Cuts at: 764 764
Size: 4376
StyI C'CwwG_G
Cuts at: 692 1089 1190 1510 1864 692
Size: 397 101 320 354 3204
Fragments arranged by size:
3204 397 354 320 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 247 984 1959 2092 2551 2760 129
Size: 118 737 975 133 459 209 1745
Fragments arranged by size:
1745 975 737 459 209 133 118
TatI w'GTAC_w
Cuts at: 3891 3891
Size: 4376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4344
Tth111I GACn'n_nGTC
Cuts at: 2173 2173
Size: 4376
XbaI T'CTAG_A
Cuts at: 1407 1407
Size: 4376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 993 1233 1871 993
Size: 240 638 3498
Fragments arranged by size:
3498 638 240
XmaI C'CCGG_G
Cuts at: 1396 1396
Size: 4376
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BcgI
BfrBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI