Restriction map for
pF1KB3350
(Circular) (Six-base) MAPSORT of: pF1KB3350.seq Check: 3708 from: 1 to: 5018
pF1KB3350 5018 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 152 152
Size: 5018
AccI GT'mk_AC
Cuts at: 2056 2056
Size: 5018
Acc65I G'GTAC_C
Cuts at: 2034 2034
Size: 5018
AclI AA'CG_TT
Cuts at: 2012 4858 2012
Size: 2846 2172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3275 3628 4290 2843
Size: 432 353 662 3571
Fragments arranged by size:
3571 662 432 353
AflIII A'CryG_T
Cuts at: 1500 2370 4170 4451 1500
Size: 870 1800 281 2067
Fragments arranged by size:
2067 1800 870 281
AleI CACnn'nnGTG
Cuts at: 1144 1144
Size: 5018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1594 1594
Size: 5018
AlwNI CAG_nnn'CTG
Cuts at: 534 1386 2323 3761 534
Size: 852 937 1438 1791
Fragments arranged by size:
1791 1438 937 852
ApaI G_GGCC'C
Cuts at: 464 464
Size: 5018
ApaLI G'TGCA_C
Cuts at: 1579 3856 1579
Size: 2277 2741
ApoI r'AATT_y
Cuts at: 2022 4371 4627 2022
Size: 2349 256 2413
Fragments arranged by size:
2413 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5018
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
AvaI C'yCGr_G
Cuts at: 170 629 793 1171 2038 170
Size: 459 164 378 867 3150
Fragments arranged by size:
3150 867 459 378 164
BamHI G'GATC_C
Cuts at: 2043 2043
Size: 5018
BanI G'GyrC_C
Cuts at: 322 1883 2034 2448 2696 2731 322
Size: 1561 151 414 248 35 2609
Fragments arranged by size:
2609 1561 414 248 151 35
BanII G_rGCy'C
Cuts at: 464 1159 1243 2032 3062 464
Size: 695 84 789 1030 2420
Fragments arranged by size:
2420 1030 789 695 84
BbeI G_GCGC'C
Cuts at: 2452 2700 2452
Size: 248 4770
BbsI GAAGACnn'nnnn_
Cuts at: 1232 1232
Size: 5018
BbvCI CC'TCA_GC
Cuts at: 563 563
Size: 5018
BciVI GTATCCnnnnn_n'
Cuts at: 2916 3972 5018 2916
Size: 1056 1046 2916
Fragments arranged by size:
2916 1056 1046
BclI T'GATC_A
Cuts at: 2538 2538
Size: 5018
BfrBI ATG'CAT
Cuts at: 1736 1736
Size: 5018
BglII A'GATC_T
Cuts at: 1587 2533 4570 1587
Size: 946 2037 2035
Fragments arranged by size:
2037 2035 946
BlpI GC'TnA_GC
Cuts at: 2130 2130
Size: 5018
Bme1580I G_kGCm'C
Cuts at: 464 1583 1888 2643 2736 3860 464
Size: 1119 305 755 93 1124 1622
Fragments arranged by size:
1622 1124 1119 755 305 93
BmgBI CAC'GTC
Cuts at: 2004 2004
Size: 5018
BmrI ACTGGGnnnn_n'
Cuts at: 467 1246 2230 2311 2646 4790 467
Size: 779 984 81 335 2144 695
Fragments arranged by size:
2144 984 779 695 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 589 1740 1794 556
Size: 33 1151 54 3780
Fragments arranged by size:
3780 1151 54 33
Bpu10I CC'TnA_GC
Cuts at: 563 563
Size: 5018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 963 2194 3073 3787 4085 400
Size: 563 1231 879 714 298 1333
Fragments arranged by size:
1333 1231 879 714 563 298
BsaI GGTCTCn'nnnn_
Cuts at: 4722 4722
Size: 5018
BsaAI yAC'GTr
Cuts at: 2371 2371
Size: 5018
BsaBI GATnn'nnATC
Cuts at: 6 2532 4569 6
Size: 2526 2037 455
Fragments arranged by size:
2526 2037 455
BsaHI Gr'CG_yC
Cuts at: 2449 2697 3399 4895 2449
Size: 248 702 1496 2572
Fragments arranged by size:
2572 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1728 2207 2728 3406 3817 3964 4238 1728
Size: 479 521 678 411 147 274 2508
Fragments arranged by size:
2508 678 521 479 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 694 724 1738 1768 367
Size: 30 297 30 1014 30 3617
Fragments arranged by size:
3617 1014 297 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 403 1189 1579 190
Size: 213 786 390 3629
Fragments arranged by size:
3629 786 390 213
BseYI C'CCAG_C
Cuts at: 2443 3201 3866 4274 2443
Size: 758 665 408 3187
Fragments arranged by size:
3187 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 715 733 466
Size: 249 18 4751
Fragments arranged by size:
4751 249 18
BsiEI CG_ry'CG
Cuts at: 104 2560 2606 3836 104
Size: 2456 46 1230 1286
Fragments arranged by size:
2456 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1583 2032 2810 3000 3860 1583
Size: 449 778 190 860 2741
Fragments arranged by size:
2741 860 778 449 190
BsmI GAATG_Cn'
Cuts at: 1738 1801 1738
Size: 63 4955
Bsp1286I G_dGCh'C
Cuts at: 464 1159 1243 1583 1888 2032 2643 2736 2810
Size: 695 84 340 305 144 611 93 74
Cuts at: 2810 3000 3062 3860 464
Size: 190 62 798 1622
Fragments arranged by size:
1622 798 695 611 340 305 190 144
93 84 74 62
BspEI T'CCGG_A
Cuts at: 1728 1728
Size: 5018
BspHI T'CATG_A
Cuts at: 1919 3450 1919
Size: 1531 3487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 152 527 2068 2282 2584 2965 152
Size: 375 1541 214 302 381 2205
Fragments arranged by size:
2205 1541 381 375 302 214
BsrBI CCG'CTC
Cuts at: 50 309 1342 1875 3310 3364 4848 5013 50
Size: 259 1033 533 1435 54 1484 165 55
Fragments arranged by size:
1484 1435 1033 533 259 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2930 2930
Size: 5018
BsrFI r'CCGG_y
Cuts at: 337 431 1043 3016 3197 337
Size: 94 612 1973 181 2158
Fragments arranged by size:
2158 1973 612 181 94
BssSI C'ACGA_G
Cuts at: 3289 3997 3289
Size: 708 4310
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 2395 1666
Size: 729 4289
BstBI TT'CG_AA
Cuts at: 3379 3379
Size: 5018
BstXI CCAn_nnnn'nTGG
Cuts at: 1699 1699
Size: 5018
BstYI r'GATC_y
Cuts at: 1 1587 2043 2510 2533 2868 3114 3420 3432
Size: 1586 456 467 23 335 246 306 12
Cuts at: 3432 3518 4570 1
Size: 86 1052 449
Fragments arranged by size:
1586 1052 467 456 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 269 276 615 1133 1301 1544 1698 107
Size: 162 7 339 518 168 243 154 3427
Fragments arranged by size:
3427 518 339 243 168 162 154 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 318 1781 2968 3149 3312 318
Size: 1463 1187 181 163 2024
Fragments arranged by size:
2024 1463 1187 181 163
BtsI GCAGTG_nn'
Cuts at: 1660 1976 2261 1660
Size: 316 285 4417
Fragments arranged by size:
4417 316 285
DraI TTT'AAA
Cuts at: 2017 2017
Size: 5018
DraIII CAC_nnn'GTG
Cuts at: 1666 1666
Size: 5018
DrdI GACnn_nn'nnGTC
Cuts at: 2724 4068 2724
Size: 1344 3674
EaeI y'GGCC_r
Cuts at: 110 1011 2603 2777 3168 3195 4540 110
Size: 901 1592 174 391 27 1345 588
Fragments arranged by size:
1592 1345 901 588 391 174 27
EagI C'GGCC_G
Cuts at: 2603 2603
Size: 5018
EarI CTCTTCn'nnn_
Cuts at: 3041 3251 3041
Size: 210 4808
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3226 3968 4114 4828 3226
Size: 742 146 714 3416
Fragments arranged by size:
3416 742 714 146
EcoICRI GAG'CTC
Cuts at: 2030 2030
Size: 5018
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 589 1740 1794 2843 3275 3628 4290 556
Size: 33 1151 54 1049 432 353 662 1284
Fragments arranged by size:
1284 1151 1049 662 432 353 54 33
EcoNI CCTnn'n_nnAGG
Cuts at: 196 1034 196
Size: 838 4180
EcoO109I rG'GnC_Cy
Cuts at: 347 461 1111 2157 347
Size: 114 650 1046 3208
Fragments arranged by size:
3208 1046 650 114
EcoRI G'AATT_C
Cuts at: 2022 2022
Size: 5018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 3037 441
Size: 2596 2422
FspI TGC'GCA
Cuts at: 1987 2799 1987
Size: 812 4206
HaeII r_GCGC'y
Cuts at: 137 2452 2700 3930 4701 137
Size: 2315 248 1230 771 454
Fragments arranged by size:
2315 1230 771 454 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1417 1449 1899 1931 2860 2890 2892 2922 1417
Size: 32 450 32 929 30 2 30 3513
Fragments arranged by size:
3513 929 450 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2057 2057
Size: 5018
KasI G'GCGC_C
Cuts at: 2448 2696 2448
Size: 248 4770
KpnI G_GTAC'C
Cuts at: 2038 2038
Size: 5018
MluI A'CGCG_T
Cuts at: 4451 4451
Size: 5018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3777 3961 3777
Size: 184 4834
MscI TGG'CCA
Cuts at: 112 1013 2779 112
Size: 901 1766 2351
Fragments arranged by size:
2351 1766 901
MslI CAynn'nnrTG
Cuts at: 1144 1549 1855 3007 3134 1144
Size: 405 306 1152 127 3028
Fragments arranged by size:
3028 1152 405 306 127
MspA1I CmG'CkG
Cuts at: 119 567 964 1386 2129 2443 2803 3587 3832
Size: 448 397 422 743 314 360 784 245
Cuts at: 3832 119
Size: 1305
Fragments arranged by size:
1305 784 743 448 422 397 360 314
245
NaeI GCC'GGC
Cuts at: 3199 3199
Size: 5018
NarI GG'CG_CC
Cuts at: 2449 2697 2449
Size: 248 4770
NcoI C'CATG_G
Cuts at: 107 615 1133 1544 1698 107
Size: 508 518 411 154 3427
Fragments arranged by size:
3427 518 508 411 154
NgoMIV G'CCGG_C
Cuts at: 3197 3197
Size: 5018
NsiI A_TGCA'T
Cuts at: 1738 1738
Size: 5018
NspI r_CATG'y
Cuts at: 1504 2071 2571 4174 1504
Size: 567 500 1603 2348
Fragments arranged by size:
2348 1603 567 500
PasI CC'CwG_GG
Cuts at: 1094 1094
Size: 5018
PciI A'CATG_T
Cuts at: 1500 4170 1500
Size: 2670 2348
PflMI CCAn_nnn'nTGG
Cuts at: 1009 1009
Size: 5018
PfoI T'CCnGG_A
Cuts at: 1435 1435
Size: 5018
PmeI GTTT'AAAC
Cuts at: 2017 2017
Size: 5018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4986
PpuMI rG'GwC_Cy
Cuts at: 347 347
Size: 5018
PsiI TTA'TAA
Cuts at: 845 845
Size: 5018
PspOMI G'GGCC_C
Cuts at: 460 460
Size: 5018
PstI C_TGCA'G
Cuts at: 524 1104 1893 2065 2750 524
Size: 580 789 172 685 2792
Fragments arranged by size:
2792 789 685 580 172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5018
PvuII CAG'CTG
Cuts at: 567 1386 2443 2803 567
Size: 819 1057 360 2782
Fragments arranged by size:
2782 1057 819 360
RsrII CG'GwC_CG
Cuts at: 3213 3213
Size: 5018
SacI G_AGCT'C
Cuts at: 2032 2032
Size: 5018
SalI G'TCGA_C
Cuts at: 2055 2055
Size: 5018
SapI GCTCTTCn'nnn_
Cuts at: 3041 3251 3041
Size: 210 4808
SbfI CC_TGCA'GG
Cuts at: 524 1893 2065 524
Size: 1369 172 3477
Fragments arranged by size:
3477 1369 172
ScaI AGT'ACT
Cuts at: 1056 1558 4535 1056
Size: 502 2977 1539
Fragments arranged by size:
2977 1539 502
SexAI A'CCwGG_T
Cuts at: 191 191
Size: 5018
SfcI C'TryA_G
Cuts at: 33 520 1100 1889 2061 2746 3714 3905 33
Size: 487 580 789 172 685 968 191 1146
Fragments arranged by size:
1146 968 789 685 580 487 191 172
SfoI GGC'GCC
Cuts at: 2450 2698 2450
Size: 248 4770
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
SgrAI Cr'CCGG_yG
Cuts at: 337 337
Size: 5018
SmaI CCC'GGG
Cuts at: 172 2040 172
Size: 1868 3150
SmlI C'TyrA_G
Cuts at: 415 978 2173 3088 3802 4064 415
Size: 563 1195 915 714 262 1369
Fragments arranged by size:
1369 1195 915 714 563 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5018
SphI G_CATG'C
Cuts at: 2071 2571 2071
Size: 500 4518
StyI C'CwwG_G
Cuts at: 107 438 607 615 1035 1133 1187 1544 1698
Size: 331 169 8 420 98 54 357 154
Cuts at: 1698 2152 2506 107
Size: 454 354 2619
Fragments arranged by size:
2619 454 420 357 354 331 169 154
98 54 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 241 1220 2601 2734 3193 3402 241
Size: 979 1381 133 459 209 1857
Fragments arranged by size:
1857 1381 979 459 209 133
TatI w'GTAC_w
Cuts at: 1054 1556 4533 1054
Size: 502 2977 1539
Fragments arranged by size:
2977 1539 502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 367 399 1006 1038 4535 4567 367
Size: 32 607 32 3497 32 818
Fragments arranged by size:
3497 818 607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 957 2815 957
Size: 1858 3160
XbaI T'CTAG_A
Cuts at: 2049 2049
Size: 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 394 2513 120
Size: 274 2119 2625
Fragments arranged by size:
2625 2119 274
XmaI C'CCGG_G
Cuts at: 170 2038 170
Size: 1868 3150
XmnI GAAnn'nnTTC
Cuts at: 424 1529 424
Size: 1105 3913
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BglI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PshAI PspXI PsrI SacII SanDI SfiI SnaBI
SrfI SspI StuI SwaI XhoI ZraI