Restriction map for
pF1KB3332
(Circular) (Six-base) MAPSORT of: pF1KB3332.seq Check: 2369 from: 1 to: 6503
pF1KB3332 6503 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 1499 2047 2058 3363 507
Size: 992 548 11 1305 3647
Fragments arranged by size:
3647 1305 992 548 11
AccI GT'mk_AC
Cuts at: 3541 3541
Size: 6503
Acc65I G'GTAC_C
Cuts at: 3519 3519
Size: 6503
AclI AA'CG_TT
Cuts at: 6343 6343
Size: 6503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1438 2070 2144 2615 2714 3212 4328 4760
Size: 549 632 74 471 99 498 1116 432
Cuts at: 4760 5507 5775 889
Size: 747 268 1617
Fragments arranged by size:
1617 1116 747 632 549 498 471 432
268 99 74
AfeI AGC'GCT
Cuts at: 3197 3197
Size: 6503
AflII C'TTAA_G
Cuts at: 1525 1525
Size: 6503
AflIII A'CryG_T
Cuts at: 1667 3855 4959 5936 1667
Size: 2188 1104 977 2234
Fragments arranged by size:
2234 2188 1104 977
AgeI A'CCGG_T
Cuts at: 4891 4891
Size: 6503
AhdI GACnn_n'nnGTC
Cuts at: 173 1245 173
Size: 1072 5431
AlwNI CAG_nnn'CTG
Cuts at: 958 2188 3490 3808 5375 958
Size: 1230 1302 318 1567 2086
Fragments arranged by size:
2086 1567 1302 1230 318
ApaI G_GGCC'C
Cuts at: 134 199 354 939 2035 2094 2116 3251 134
Size: 65 155 585 1096 59 22 1135 3386
Fragments arranged by size:
3386 1135 1096 585 155 65 59 22
ApaLI G'TGCA_C
Cuts at: 3032 5273 3032
Size: 2241 4262
ApoI r'AATT_y
Cuts at: 2908 3507 5856 6112 2908
Size: 599 2349 256 3299
Fragments arranged by size:
3299 2349 599 256
AseI AT'TA_AT
Cuts at: 20 2750 20
Size: 2730 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
AvaI C'yCGr_G
Cuts at: 192 307 728 2006 2482 3115 3523 192
Size: 115 421 1278 476 633 408 3172
Fragments arranged by size:
3172 1278 633 476 421 408 115
AvrII C'CTAG_G
Cuts at: 1071 1800 2232 1071
Size: 729 432 5342
Fragments arranged by size:
5342 729 432
BamHI G'GATC_C
Cuts at: 1292 3528 1292
Size: 2236 4267
BanI G'GyrC_C
Cuts at: 2521 3519 3933 4181 4216 2521
Size: 998 414 248 35 4808
Fragments arranged by size:
4808 998 414 248 35
BanII G_rGCy'C
Cuts at: 134 145 193 199 314 354 466 796 939
Size: 11 48 6 115 40 112 330 143
Cuts at: 939 1079 1104 1552 1825 2035 2094 2116 2134
Size: 140 25 448 273 210 59 22 18
Cuts at: 2134 3251 3281 3517 4547 134
Size: 1117 30 236 1030 2090
Fragments arranged by size:
2090 1117 1030 448 330 273 236 210
143 140 115 112 59 48 40 30
25 22 18 11 6
BbeI G_GCGC'C
Cuts at: 3937 4185 3937
Size: 248 6255
BbsI GAAGACnn'nnnn_
Cuts at: 2837 2996 2837
Size: 159 6344
BbvCI CC'TCA_GC
Cuts at: 797 1153 1693 2142 2178 797
Size: 356 540 449 36 5122
Fragments arranged by size:
5122 540 449 356 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 173 207 1654 1688 173
Size: 34 1447 34 4988
Fragments arranged by size:
4988 1447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4401 5162 6503 4401
Size: 761 1341 4401
Fragments arranged by size:
4401 1341 761
BclI T'GATC_A
Cuts at: 4023 4023
Size: 6503
BglI GCCn_nnn'nGGC
Cuts at: 112 2487 3433 112
Size: 2375 946 3182
Fragments arranged by size:
3182 2375 946
BglII A'GATC_T
Cuts at: 2438 4018 6055 2438
Size: 1580 2037 2886
Fragments arranged by size:
2886 2037 1580
BlpI GC'TnA_GC
Cuts at: 1084 1495 3615 1084
Size: 411 2120 3972
Fragments arranged by size:
3972 2120 411
Bme1580I G_kGCm'C
Cuts at: 134 199 354 939 1911 2035 2094 2116 2195
Size: 65 155 585 972 124 59 22 79
Cuts at: 2195 2390 2483 3036 3251 4128 4221 5277 134
Size: 195 93 553 215 877 93 1056 1360
Fragments arranged by size:
1360 1056 972 877 585 553 215 195
155 124 93 93 79 65 59 22
BmgBI CAC'GTC
Cuts at: 400 1668 400
Size: 1268 5235
BmrI ACTGGGnnnn_n'
Cuts at: 1580 2558 2676 3715 3796 4131 6275 1580
Size: 978 118 1039 81 335 2144 1808
Fragments arranged by size:
2144 1808 1039 978 335 118 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1369 1974 3510 4870 1369
Size: 605 1536 1360 3002
Fragments arranged by size:
3002 1536 1360 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1018 1343 1353 1490 2039 3016 3041 3382
Size: 777 325 10 137 549 977 25 341
Cuts at: 3382 241
Size: 3362
Fragments arranged by size:
3362 977 777 549 341 325 137 25
10
Bpu10I CC'TnA_GC
Cuts at: 797 1153 1693 2142 2178 797
Size: 356 540 449 36 5122
Fragments arranged by size:
5122 540 449 356 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1249 1969 3679 4558 5050 5348 810
Size: 439 720 1710 879 492 298 1965
Fragments arranged by size:
1965 1710 879 720 492 439 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 1959 6207 181
Size: 1778 4248 477
Fragments arranged by size:
4248 1778 477
BsaAI yAC'GTr
Cuts at: 3856 3856
Size: 6503
BsaBI GATnn'nnATC
Cuts at: 6 1297 4017 6054 6
Size: 1291 2720 2037 455
Fragments arranged by size:
2720 2037 1291 455
BsaHI Gr'CG_yC
Cuts at: 3934 4182 4884 6380 3934
Size: 248 702 1496 4057
Fragments arranged by size:
4057 1496 702 248
BsaWI w'CCGG_w
Cuts at: 270 1637 3692 4213 4891 5165 5312 5723 270
Size: 1367 2055 521 678 274 147 411 1050
Fragments arranged by size:
2055 1367 1050 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1091 1121 1326 1356 1807 1837 2037 2067 2658
Size: 30 205 30 451 30 200 30 591
Cuts at: 2658 2688 3005 3035 3039 3069 1091
Size: 30 317 30 4 30 4525
Fragments arranged by size:
4525 591 451 317 205 200 30 30
30 30 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 571 1475 1747 1871 1886 1947 2434 2602 2788
Size: 904 272 124 15 61 487 168 186
Cuts at: 2788 2791 2839 2899 2965 2974 2977 3151 3166
Size: 3 48 60 66 9 3 174 15
Cuts at: 3166 3169 3322 571
Size: 3 153 3752
Fragments arranged by size:
3752 904 487 272 186 174 168 153
124 66 61 60 48 15 15 9
3 3 3
BseYI C'CCAG_C
Cuts at: 151 596 660 884 930 2721 3928 4686 5263
Size: 445 64 224 46 1791 1207 758 577
Cuts at: 5263 5759 151
Size: 496 895
Fragments arranged by size:
1791 1207 895 758 577 496 445 224
64 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 904 988 1141 1235 1444 1664 3112 3355
Size: 314 84 153 94 209 220 1448 243
Cuts at: 3355 590
Size: 3738
Fragments arranged by size:
3738 1448 314 243 220 209 153 94
84
BsiEI CG_ry'CG
Cuts at: 104 1305 4045 4091 5299 104
Size: 1201 2740 46 1208 1308
Fragments arranged by size:
2740 1308 1208 1201 46
BsiHKAI G_wGCw'C
Cuts at: 193 1104 1825 3036 3517 4295 4485 5277 193
Size: 911 721 1211 481 778 190 792 1419
Fragments arranged by size:
1419 1211 911 792 778 721 481 190
BsmI GAATG_Cn'
Cuts at: 509 1454 509
Size: 945 5558
BsmBI CGTCTCn'nnnn_
Cuts at: 406 1883 406
Size: 1477 5026
Bsp1286I G_dGCh'C
Cuts at: 134 145 193 199 314 354 466 796 939
Size: 11 48 6 115 40 112 330 143
Cuts at: 939 1079 1104 1552 1825 1911 2035 2094 2116
Size: 140 25 448 273 86 124 59 22
Cuts at: 2116 2134 2195 2390 2483 3036 3251 3281 3517
Size: 18 61 195 93 553 215 30 236
Cuts at: 3517 4128 4221 4295 4485 4547 5277 134
Size: 611 93 74 190 62 730 1360
Fragments arranged by size:
1360 730 611 553 448 330 273 236
215 195 190 143 140 124 115 112
93 93 86 74 62 61 59 48
40 30 25 22 18 11 6
BspEI T'CCGG_A
Cuts at: 5723 5723
Size: 6503
BspHI T'CATG_A
Cuts at: 5679 5679
Size: 6503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 507 1250 1416 1499 2047 2058 3363 3553 3767
Size: 743 166 83 548 11 1305 190 214
Cuts at: 3767 4069 4450 507
Size: 302 381 2560
Fragments arranged by size:
2560 1305 743 548 381 302 214 190
166 83 11
BsrBI CCG'CTC
Cuts at: 50 290 4795 4849 6333 6498 50
Size: 240 4505 54 1484 165 55
Fragments arranged by size:
4505 1484 240 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1659 4415 1659
Size: 2756 3747
BsrFI r'CCGG_y
Cuts at: 1274 4501 4682 4891 1274
Size: 3227 181 209 2886
Fragments arranged by size:
3227 2886 209 181
BsrGI T'GTAC_A
Cuts at: 1782 1782
Size: 6503
BssSI C'ACGA_G
Cuts at: 1818 4774 5132 1818
Size: 2956 358 3189
Fragments arranged by size:
3189 2956 358
BstAPI GCAn_nnn'nTGC
Cuts at: 927 958 3880 927
Size: 31 2922 3550
Fragments arranged by size:
3550 2922 31
BstBI TT'CG_AA
Cuts at: 4864 4864
Size: 6503
BstEII G'GTnAC_C
Cuts at: 1980 1980
Size: 6503
BstXI CCAn_nnnn'nTGG
Cuts at: 640 640
Size: 6503
BstYI r'GATC_y
Cuts at: 1 1292 2438 3528 3995 4018 4353 4599 5611
Size: 1291 1146 1090 467 23 335 246 1012
Cuts at: 5611 5697 5709 6055 1
Size: 86 12 346 449
Fragments arranged by size:
1291 1146 1090 1012 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 571 667 2069 2517 571
Size: 96 1402 448 4557
Fragments arranged by size:
4557 1402 448 96
BtgI C'CryG_G
Cuts at: 107 146 199 560 1540 2027 107
Size: 39 53 361 980 487 4583
Fragments arranged by size:
4583 980 487 361 53 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2063 4453 4634 4797 2063
Size: 2390 181 163 3769
Fragments arranged by size:
3769 2390 181 163
BtsI GCAGTG_nn'
Cuts at: 191 916 2769 3746 191
Size: 725 1853 977 2948
Fragments arranged by size:
2948 1853 977 725
ClaI AT'CG_AT
Cuts at: 3446 3446
Size: 6503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2666 2701 2666
Size: 35 6468
DraI TTT'AAA
Cuts at: 3502 3502
Size: 6503
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 6503
DrdI GACnn_nn'nnGTC
Cuts at: 4209 5067 4209
Size: 858 5645
EaeI y'GGCC_r
Cuts at: 557 1302 1929 3434 4088 4262 4653 4680 6025
Size: 745 627 1505 654 174 391 27 1345
Cuts at: 6025 557
Size: 1035
Fragments arranged by size:
1505 1345 1035 745 654 627 391 174
27
EagI C'GGCC_G
Cuts at: 1302 4088 1302
Size: 2786 3717
EarI CTCTTCn'nnn_
Cuts at: 2726 2765 2777 2786 2801 2810 2816 2837 2843
Size: 39 12 9 15 9 6 21 6
Cuts at: 2843 2852 2867 2876 2888 2942 2975 3269 3299
Size: 9 15 9 12 54 33 294 30
Cuts at: 3299 3359 4526 4736 2726
Size: 60 1167 210 4493
Fragments arranged by size:
4493 1167 294 210 60 54 39 33
30 21 15 15 12 12 9 9
9 9 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 2630 4711 5021 5167 6313 196
Size: 2434 2081 310 146 1146 386
Fragments arranged by size:
2434 2081 1146 386 310 146
EcoICRI GAG'CTC
Cuts at: 191 1102 1823 3515 191
Size: 911 721 1692 3179
Fragments arranged by size:
3179 1692 911 721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 889 1018 1343 1353 1438 1490 2039 2070
Size: 648 129 325 10 85 52 549 31
Cuts at: 2070 2144 2615 2714 3016 3041 3212 3382 4328
Size: 74 471 99 302 25 171 170 946
Cuts at: 4328 4760 5507 5775 241
Size: 432 747 268 969
Fragments arranged by size:
969 946 747 648 549 471 432 325
302 268 171 170 129 99 85 74
52 31 25 10
EcoNI CCTnn'n_nnAGG
Cuts at: 943 2162 2731 3242 943
Size: 1219 569 511 4204
Fragments arranged by size:
4204 1219 569 511
EcoO109I rG'GnC_Cy
Cuts at: 131 350 429 534 576 935 936 979 1320
Size: 219 79 105 42 359 1 43 341
Cuts at: 1320 1436 1476 1797 2032 2073 2090 2091 2113
Size: 116 40 321 235 41 17 1 22
Cuts at: 2113 2149 2167 2185 2316 2617 2716 3247 3248
Size: 36 18 18 131 301 99 531 1
Cuts at: 3248 3642 131
Size: 394 2992
Fragments arranged by size:
2992 531 394 359 341 321 301 235
219 131 116 105 99 79 43 42
41 40 36 22 18 18 17 1
1 1
EcoRI G'AATT_C
Cuts at: 3507 3507
Size: 6503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 4522 1963
Size: 2559 3944
FspI TGC'GCA
Cuts at: 4284 4284
Size: 6503
HaeII r_GCGC'y
Cuts at: 1586 1864 3199 3937 4185 5207 6186 1586
Size: 278 1335 738 248 1022 979 1903
Fragments arranged by size:
1903 1335 1022 979 738 278 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 1231 1263 1339 1371 2062 2094 2410
Size: 32 1040 32 76 32 691 32 316
Cuts at: 2410 2442 2658 2690 4345 4375 4377 4407 159
Size: 32 216 32 1655 30 2 30 2255
Fragments arranged by size:
2255 1655 1040 691 316 216 76 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3542 3542
Size: 6503
KasI G'GCGC_C
Cuts at: 3933 4181 3933
Size: 248 6255
KpnI G_GTAC'C
Cuts at: 3523 3523
Size: 6503
MluI A'CGCG_T
Cuts at: 5936 5936
Size: 6503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 828 3281 5174 5358 828
Size: 2453 1893 184 1973
Fragments arranged by size:
2453 1973 1893 184
MscI TGG'CCA
Cuts at: 4264 4264
Size: 6503
MslI CAynn'nnrTG
Cuts at: 625 1187 1708 4492 4619 625
Size: 562 521 2784 127 2509
Fragments arranged by size:
2784 2509 562 521 127
MspA1I CmG'CkG
Cuts at: 148 201 600 1067 1516 1542 1829 3424 3614
Size: 53 399 467 449 26 287 1595 190
Cuts at: 3614 3928 4288 5301 5546 148
Size: 314 360 1013 245 1105
Fragments arranged by size:
1595 1105 1013 467 449 399 360 314
287 245 190 53 26
NaeI GCC'GGC
Cuts at: 1276 4684 1276
Size: 3408 3095
NarI GG'CG_CC
Cuts at: 3934 4182 3934
Size: 248 6255
NcoI C'CATG_G
Cuts at: 107 2027 107
Size: 1920 4583
NgoMIV G'CCGG_C
Cuts at: 1274 4682 1274
Size: 3408 3095
NspI r_CATG'y
Cuts at: 3556 4056 4963 3556
Size: 500 907 5096
Fragments arranged by size:
5096 907 500
PasI CC'CwG_GG
Cuts at: 982 2614 3207 982
Size: 1632 593 4278
Fragments arranged by size:
4278 1632 593
PciI A'CATG_T
Cuts at: 4959 4959
Size: 6503
PflMI CCAn_nnn'nTGG
Cuts at: 810 810
Size: 6503
PfoI T'CCnGG_A
Cuts at: 614 1130 1330 3398 614
Size: 516 200 2068 3719
Fragments arranged by size:
3719 2068 516 200
PmeI GTTT'AAAC
Cuts at: 3502 3502
Size: 6503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 5642
Size: 32 6471
PpuMI rG'GwC_Cy
Cuts at: 534 576 1320 1797 2316 2716 534
Size: 42 744 477 519 400 4321
Fragments arranged by size:
4321 744 519 477 400 42
PspOMI G'GGCC_C
Cuts at: 130 195 350 935 2031 2090 2112 3247 130
Size: 65 155 585 1096 59 22 1135 3386
Fragments arranged by size:
3386 1135 1096 585 155 65 59 22
PstI C_TGCA'G
Cuts at: 2024 2610 3360 3550 4235 2024
Size: 586 750 190 685 4292
Fragments arranged by size:
4292 750 685 586 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6503
PvuII CAG'CTG
Cuts at: 600 1516 3424 3928 4288 600
Size: 916 1908 504 360 2815
Fragments arranged by size:
2815 1908 916 504 360
RsrII CG'GwC_CG
Cuts at: 4698 4698
Size: 6503
SacI G_AGCT'C
Cuts at: 193 1104 1825 3517 193
Size: 911 721 1692 3179
Fragments arranged by size:
3179 1692 911 721
SacII CC_GC'GG
Cuts at: 149 202 1543 149
Size: 53 1341 5109
Fragments arranged by size:
5109 1341 53
SalI G'TCGA_C
Cuts at: 3540 3540
Size: 6503
SapI GCTCTTCn'nnn_
Cuts at: 3269 4526 4736 3269
Size: 1257 210 5036
Fragments arranged by size:
5036 1257 210
SbfI CC_TGCA'GG
Cuts at: 3550 3550
Size: 6503
ScaI AGT'ACT
Cuts at: 6020 6020
Size: 6503
SexAI A'CCwGG_T
Cuts at: 1757 1757
Size: 6503
SfcI C'TryA_G
Cuts at: 33 2020 2537 2606 3356 3546 4231 5224 5415
Size: 1987 517 69 750 190 685 993 191
Cuts at: 5415 33
Size: 1121
Fragments arranged by size:
1987 1121 993 750 685 517 191 190
69
SfoI GGC'GCC
Cuts at: 3935 4183 3935
Size: 248 6255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6503
SmaI CCC'GGG
Cuts at: 309 2008 3117 3525 309
Size: 1699 1109 408 3287
Fragments arranged by size:
3287 1699 1109 408
SmlI C'TyrA_G
Cuts at: 789 1228 1525 1948 3658 4573 5065 5327 789
Size: 439 297 423 1710 915 492 262 1965
Fragments arranged by size:
1965 1710 915 492 439 423 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6503
SphI G_CATG'C
Cuts at: 3556 4056 3556
Size: 500 6003
SrfI GCCC'GGGC
Cuts at: 309 309
Size: 6503
SspI AAT'ATT
Cuts at: 1173 2866 1173
Size: 1693 4810
StuI AGG'CCT
Cuts at: 866 1234 1374 1691 2237 2252 866
Size: 368 140 317 546 15 5117
Fragments arranged by size:
5117 546 368 317 140 15
StyI C'CwwG_G
Cuts at: 107 861 1071 1235 1394 1800 2027 2232 2444
Size: 754 210 164 159 406 227 205 212
Cuts at: 2444 3637 3991 107
Size: 1193 354 2619
Fragments arranged by size:
2619 1193 754 406 354 227 212 210
205 164 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 1501 1768 4086 4219 4678 4887 1410
Size: 91 267 2318 133 459 209 3026
Fragments arranged by size:
3026 2318 459 267 209 133 91
TatI w'GTAC_w
Cuts at: 1782 6018 1782
Size: 4236 2267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1091 1123 2037 2069 2691 2723 6020 6052 1091
Size: 32 914 32 622 32 3297 32 1542
Fragments arranged by size:
3297 1542 914 622 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1759 4300 1759
Size: 2541 3962
XbaI T'CTAG_A
Cuts at: 3534 3534
Size: 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 2124 3998 552
Size: 1572 1874 3057
Fragments arranged by size:
3057 1874 1572
XmaI C'CCGG_G
Cuts at: 307 2006 3115 3523 307
Size: 1699 1109 408 3287
Fragments arranged by size:
3287 1699 1109 408
XmnI GAAnn'nnTTC
Cuts at: 2600 2600
Size: 6503
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AleI AlfI AloI AscI BaeI BfrBI BmtI
BsiWI BssHII BstZ17I EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SwaI XhoI ZraI