Restriction map for
pF1KB3329
(Circular) (Six-base) MAPSORT of: pF1KB3329.seq Check: 4812 from: 1 to: 5960
pF1KB3329 5960 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1749 1749
Size: 5960
AccI GT'mk_AC
Cuts at: 2257 2998 2257
Size: 741 5219
Acc65I G'GTAC_C
Cuts at: 2976 2976
Size: 5960
AclI AA'CG_TT
Cuts at: 5800 5800
Size: 5960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1169 2403 3785 4217 4964 5232 1169
Size: 1234 1382 432 747 268 1897
Fragments arranged by size:
1897 1382 1234 747 432 268
AflIII A'CryG_T
Cuts at: 1355 1625 1916 2005 2195 3312 4416 5393 1355
Size: 270 291 89 190 1117 1104 977 1922
Fragments arranged by size:
1922 1117 1104 977 291 270 190 89
AgeI A'CCGG_T
Cuts at: 4348 4348
Size: 5960
AleI CACnn'nnGTG
Cuts at: 238 2320 238
Size: 2082 3878
AlwNI CAG_nnn'CTG
Cuts at: 261 294 2176 3265 4832 261
Size: 33 1882 1089 1567 1389
Fragments arranged by size:
1882 1567 1389 1089 33
ApaI G_GGCC'C
Cuts at: 2395 2395
Size: 5960
ApaLI G'TGCA_C
Cuts at: 1633 2563 4730 1633
Size: 930 2167 2863
Fragments arranged by size:
2863 2167 930
ApoI r'AATT_y
Cuts at: 172 366 2764 2964 5313 5569 172
Size: 194 2398 200 2349 256 563
Fragments arranged by size:
2398 2349 563 256 200 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 5960
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
AvaI C'yCGr_G
Cuts at: 1477 2980 1477
Size: 1503 4457
BamHI G'GATC_C
Cuts at: 2985 2985
Size: 5960
BanI G'GyrC_C
Cuts at: 136 232 778 2127 2158 2976 3390 3638 3673
Size: 96 546 1349 31 818 414 248 35
Cuts at: 3673 136
Size: 2423
Fragments arranged by size:
2423 1349 818 546 414 248 96 35
31
BanII G_rGCy'C
Cuts at: 2170 2395 2974 4004 2170
Size: 225 579 1030 4126
Fragments arranged by size:
4126 1030 579 225
BbeI G_GCGC'C
Cuts at: 2131 2162 3394 3642 2131
Size: 31 1232 248 4449
Fragments arranged by size:
4449 1232 248 31
BbsI GAAGACnn'nnnn_
Cuts at: 978 2422 978
Size: 1444 4516
BciVI GTATCCnnnnn_n'
Cuts at: 1437 2640 3858 4619 5960 1437
Size: 1203 1218 761 1341 1437
Fragments arranged by size:
1437 1341 1218 1203 761
BclI T'GATC_A
Cuts at: 1217 2204 2610 3480 1217
Size: 987 406 870 3697
Fragments arranged by size:
3697 987 870 406
BglII A'GATC_T
Cuts at: 3475 5512 3475
Size: 2037 3923
BlpI GC'TnA_GC
Cuts at: 1193 3072 1193
Size: 1879 4081
Bme1580I G_kGCm'C
Cuts at: 139 1637 2013 2395 2567 3585 3678 4734 139
Size: 1498 376 382 172 1018 93 1056 1365
Fragments arranged by size:
1498 1365 1056 1018 382 376 172 93
BmgBI CAC'GTC
Cuts at: 1446 1446
Size: 5960
BmrI ACTGGGnnnn_n'
Cuts at: 1171 2420 3172 3253 3588 5732 1171
Size: 1249 752 81 335 2144 1399
Fragments arranged by size:
2144 1399 1249 752 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 4327 2043
Size: 2284 3676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 1708 2657 289
Size: 63 93 1263 949 3592
Fragments arranged by size:
3592 1263 949 93 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3136 4015 4507 4805 3136
Size: 879 492 298 4291
Fragments arranged by size:
4291 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2860 5664 2860
Size: 2804 3156
BsaAI yAC'GTr
Cuts at: 1626 1872 3313 1626
Size: 246 1441 4273
Fragments arranged by size:
4273 1441 246
BsaBI GATnn'nnATC
Cuts at: 6 3474 5511 6
Size: 3468 2037 455
Fragments arranged by size:
3468 2037 455
BsaHI Gr'CG_yC
Cuts at: 307 1273 2128 2159 3391 3639 4341 5837 307
Size: 966 855 31 1232 248 702 1496 430
Fragments arranged by size:
1496 1232 966 855 702 430 248 31
BsaWI w'CCGG_w
Cuts at: 723 2740 3149 3670 4348 4622 4769 5180 723
Size: 2017 409 521 678 274 147 411 1503
Fragments arranged by size:
2017 1503 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1582 1612 1817 1847 2464 2494 2518 2548 1582
Size: 30 205 30 617 30 24 30 4994
Fragments arranged by size:
4994 617 205 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 232 400 1696 2401 2467 2587 2878
Size: 3 75 168 1296 705 66 120 291
Cuts at: 2878 154
Size: 3236
Fragments arranged by size:
3236 1296 705 291 168 120 75 66
3
BseYI C'CCAG_C
Cuts at: 1198 3385 4143 4720 5216 1198
Size: 2187 758 577 496 1942
Fragments arranged by size:
2187 1942 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 432 307
Size: 125 5835
BsiEI CG_ry'CG
Cuts at: 104 381 1608 1855 3502 3548 4756 104
Size: 277 1227 247 1647 46 1208 1308
Fragments arranged by size:
1647 1308 1227 1208 277 247 46
BsiHKAI G_wGCw'C
Cuts at: 893 1637 2329 2383 2567 2974 3752 3942 4734
Size: 744 692 54 184 407 778 190 792
Cuts at: 4734 893
Size: 2119
Fragments arranged by size:
2119 792 778 744 692 407 190 184
54
BsmI GAATG_Cn'
Cuts at: 606 2582 606
Size: 1976 3984
BsmBI CGTCTCn'nnnn_
Cuts at: 216 908 216
Size: 692 5268
Bsp1286I G_dGCh'C
Cuts at: 139 893 1637 2013 2170 2329 2383 2395 2567
Size: 754 744 376 157 159 54 12 172
Cuts at: 2567 2974 3585 3678 3752 3942 4004 4734 139
Size: 407 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 754 744 730 611 407 376 190
172 159 157 93 74 62 54 12
BspEI T'CCGG_A
Cuts at: 723 5180 723
Size: 4457 1503
BspHI T'CATG_A
Cuts at: 5136 5136
Size: 5960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 1087 1270 1749 3010 3224 3526 3907 441
Size: 646 183 479 1261 214 302 381 2494
Fragments arranged by size:
2494 1261 646 479 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 191 1593 1646 2120 4252 4306 5790 5955
Size: 141 1402 53 474 2132 54 1484 165
Cuts at: 5955 50
Size: 55
Fragments arranged by size:
2132 1484 1402 474 165 141 55 54
53
BsrDI GCAATG_nn'
Cuts at: 1208 3872 1208
Size: 2664 3296
BsrFI r'CCGG_y
Cuts at: 498 1791 1941 2073 3958 4139 4348 498
Size: 1293 150 132 1885 181 209 2110
Fragments arranged by size:
2110 1885 1293 209 181 150 132
BsrGI T'GTAC_A
Cuts at: 1105 1982 1105
Size: 877 5083
BssHII G'CGCG_C
Cuts at: 1610 1712 1714 1887 2300 1610
Size: 102 2 173 413 5270
Fragments arranged by size:
5270 413 173 102 2
BssSI C'ACGA_G
Cuts at: 1678 4231 4589 1678
Size: 2553 358 3049
Fragments arranged by size:
3049 2553 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1897 3337 1897
Size: 1440 4520
BstBI TT'CG_AA
Cuts at: 4321 4321
Size: 5960
BstEII G'GTnAC_C
Cuts at: 1257 1257
Size: 5960
BstXI CCAn_nnnn'nTGG
Cuts at: 2100 2220 2235 2100
Size: 120 15 5825
Fragments arranged by size:
5825 120 15
BstYI r'GATC_y
Cuts at: 1 2779 2985 3452 3475 3810 4056 5068 5154
Size: 2778 206 467 23 335 246 1012 86
Cuts at: 5154 5166 5512 1
Size: 12 346 449
Fragments arranged by size:
2778 1012 467 449 346 335 246 206
86 23 12
BstZ17I GTA'TAC
Cuts at: 2258 2258
Size: 5960
BtgI C'CryG_G
Cuts at: 1415 1430 1849 2087 1415
Size: 15 419 238 5288
Fragments arranged by size:
5288 419 238 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 1733 2193 3910 4091 4254 234
Size: 1499 460 1717 181 163 1940
Fragments arranged by size:
1940 1717 1499 460 181 163
BtsI GCAGTG_nn'
Cuts at: 1795 1898 2623 3203 1795
Size: 103 725 580 4552
Fragments arranged by size:
4552 725 580 103
DraI TTT'AAA
Cuts at: 695 2712 2959 695
Size: 2017 247 3696
Fragments arranged by size:
3696 2017 247
DraIII CAC_nnn'GTG
Cuts at: 1875 1875
Size: 5960
DrdI GACnn_nn'nnGTC
Cuts at: 922 1167 1243 3666 4524 922
Size: 245 76 2423 858 2358
Fragments arranged by size:
2423 2358 858 245 76
EaeI y'GGCC_r
Cuts at: 192 1418 1439 2084 2090 2620 3545 3719 4110
Size: 1226 21 645 6 530 925 174 391
Cuts at: 4110 4137 5482 192
Size: 27 1345 670
Fragments arranged by size:
1345 1226 925 670 645 530 391 174
27 21 6
EagI C'GGCC_G
Cuts at: 3545 3545
Size: 5960
EarI CTCTTCn'nnn_
Cuts at: 590 2145 3983 4193 590
Size: 1555 1838 210 2357
Fragments arranged by size:
2357 1838 1555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 4168 4478 4624 5770 292
Size: 3876 310 146 1146 482
Fragments arranged by size:
3876 1146 482 310 146
EcoICRI GAG'CTC
Cuts at: 2972 2972
Size: 5960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 1169 1708 2403 2657 3785 4217
Size: 63 93 724 539 695 254 1128 432
Cuts at: 4217 4964 5232 289
Size: 747 268 1017
Fragments arranged by size:
1128 1017 747 724 695 539 432 268
254 93 63
EcoNI CCTnn'n_nnAGG
Cuts at: 331 2017 2572 331
Size: 1686 555 3719
Fragments arranged by size:
3719 1686 555
EcoO109I rG'GnC_Cy
Cuts at: 258 324 2391 2605 3099 258
Size: 66 2067 214 494 3119
Fragments arranged by size:
3119 2067 494 214 66
EcoRI G'AATT_C
Cuts at: 172 366 2964 172
Size: 194 2598 3168
Fragments arranged by size:
3168 2598 194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 901 3979 901
Size: 3078 2882
FspI TGC'GCA
Cuts at: 3741 3741
Size: 5960
HaeII r_GCGC'y
Cuts at: 294 1340 2080 2131 2162 2266 3394 3642 4664
Size: 1046 740 51 31 104 1128 248 1022
Cuts at: 4664 5643 294
Size: 979 611
Fragments arranged by size:
1128 1046 1022 979 740 611 248 104
51 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 1222 1254 1888 1920 2464 2496 3802
Size: 32 1053 32 634 32 544 32 1306
Cuts at: 3802 3832 3834 3864 137
Size: 30 2 30 2233
Fragments arranged by size:
2233 1306 1053 634 544 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 813 1248 1836 2193 2691 2999 813
Size: 435 588 357 498 308 3774
Fragments arranged by size:
3774 588 498 435 357 308
HpaI GTT'AAC
Cuts at: 1836 1836
Size: 5960
KasI G'GCGC_C
Cuts at: 2127 2158 3390 3638 2127
Size: 31 1232 248 4449
Fragments arranged by size:
4449 1232 248 31
KpnI G_GTAC'C
Cuts at: 2980 2980
Size: 5960
MluI A'CGCG_T
Cuts at: 1916 2005 5393 1916
Size: 89 3388 2483
Fragments arranged by size:
3388 2483 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1652 4631 4815 1652
Size: 2979 184 2797
Fragments arranged by size:
2979 2797 184
MscI TGG'CCA
Cuts at: 2622 3721 2622
Size: 1099 4861
MslI CAynn'nnrTG
Cuts at: 238 2218 2320 3949 4076 238
Size: 1980 102 1629 127 2122
Fragments arranged by size:
2122 1980 1629 127 102
MspA1I CmG'CkG
Cuts at: 447 1851 2702 3071 3385 3745 4758 5003 447
Size: 1404 851 369 314 360 1013 245 1404
Fragments arranged by size:
1404 1404 1013 851 369 360 314 245
NaeI GCC'GGC
Cuts at: 1793 1943 2075 4141 1793
Size: 150 132 2066 3612
Fragments arranged by size:
3612 2066 150 132
NarI GG'CG_CC
Cuts at: 2128 2159 3391 3639 2128
Size: 31 1232 248 4449
Fragments arranged by size:
4449 1232 248 31
NdeI CA'TA_TG
Cuts at: 1929 1929
Size: 5960
NgoMIV G'CCGG_C
Cuts at: 1791 1941 2073 4139 1791
Size: 150 132 2066 3612
Fragments arranged by size:
3612 2066 150 132
NspI r_CATG'y
Cuts at: 2553 3013 3513 4420 2553
Size: 460 500 907 4093
Fragments arranged by size:
4093 907 500 460
PciI A'CATG_T
Cuts at: 4416 4416
Size: 5960
PflMI CCAn_nnn'nTGG
Cuts at: 661 804 1875 661
Size: 143 1071 4746
Fragments arranged by size:
4746 1071 143
PfoI T'CCnGG_A
Cuts at: 2143 2598 2143
Size: 455 5505
PmeI GTTT'AAAC
Cuts at: 2959 2959
Size: 5960
PmlI CAC'GTG
Cuts at: 1872 1872
Size: 5960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5099 5131 5099
Size: 32 5928
PpuMI rG'GwC_Cy
Cuts at: 258 324 258
Size: 66 5894
PsiI TTA'TAA
Cuts at: 1052 1052
Size: 5960
PspOMI G'GGCC_C
Cuts at: 2391 2391
Size: 5960
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1474 1506 1474
Size: 32 5928
PstI C_TGCA'G
Cuts at: 347 452 1667 1760 3007 3692 347
Size: 105 1215 93 1247 685 2615
Fragments arranged by size:
2615 1247 1215 685 105 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5960
PvuII CAG'CTG
Cuts at: 2702 3385 3745 2702
Size: 683 360 4917
Fragments arranged by size:
4917 683 360
RsrII CG'GwC_CG
Cuts at: 4155 4155
Size: 5960
SacI G_AGCT'C
Cuts at: 2974 2974
Size: 5960
SacII CC_GC'GG
Cuts at: 1852 1852
Size: 5960
SalI G'TCGA_C
Cuts at: 2997 2997
Size: 5960
SapI GCTCTTCn'nnn_
Cuts at: 3983 4193 3983
Size: 210 5750
SbfI CC_TGCA'GG
Cuts at: 3007 3007
Size: 5960
ScaI AGT'ACT
Cuts at: 2051 5477 2051
Size: 3426 2534
SexAI A'CCwGG_T
Cuts at: 2951 2951
Size: 5960
SfcI C'TryA_G
Cuts at: 33 148 343 448 615 749 1070 1663 1696
Size: 115 195 105 167 134 321 593 33
Cuts at: 1696 1756 3003 3688 4681 4872 33
Size: 60 1247 685 993 191 1121
Fragments arranged by size:
1247 1121 993 685 593 321 195 191
167 134 115 105 60 33
SfoI GGC'GCC
Cuts at: 2129 2160 3392 3640 2129
Size: 31 1232 248 4449
Fragments arranged by size:
4449 1232 248 31
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
SgrAI Cr'CCGG_yG
Cuts at: 498 498
Size: 5960
SmaI CCC'GGG
Cuts at: 2982 2982
Size: 5960
SmlI C'TyrA_G
Cuts at: 3115 4030 4522 4784 3115
Size: 915 492 262 4291
Fragments arranged by size:
4291 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5960
SphI G_CATG'C
Cuts at: 2553 3013 3513 2553
Size: 460 500 5000
Fragments arranged by size:
5000 500 460
StuI AGG'CCT
Cuts at: 2137 2137
Size: 5960
StyI C'CwwG_G
Cuts at: 3094 3448 3094
Size: 354 5606
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 926 1104 1849 3543 3676 4135 4344 926
Size: 178 745 1694 133 459 209 2542
Fragments arranged by size:
2542 1694 745 459 209 178 133
TatI w'GTAC_w
Cuts at: 1062 1105 1481 1982 2049 5475 1062
Size: 43 376 501 67 3426 1547
Fragments arranged by size:
3426 1547 501 376 67 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5477 5509 5477
Size: 32 5928
Tth111I GACn'n_nGTC
Cuts at: 3757 3757
Size: 5960
XbaI T'CTAG_A
Cuts at: 2991 2991
Size: 5960
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1428 3455 1428
Size: 2027 3933
XmaI C'CCGG_G
Cuts at: 2980 2980
Size: 5960
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII MfeI
NcoI NheI NotI NruI NsiI PacI PasI PshAI
PspXI SanDI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI