Restriction map for
pF1KB3327
(Circular) (Six-base) MAPSORT of: pF1KB3327.seq Check: 634 from: 1 to: 5798
pF1KB3327 5798 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 941 1807 941
Size: 866 4932
AccI GT'mk_AC
Cuts at: 2719 2836 2719
Size: 117 5681
Acc65I G'GTAC_C
Cuts at: 145 2814 145
Size: 2669 3129
AclI AA'CG_TT
Cuts at: 5638 5638
Size: 5798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 2681 3623 4055 4408 5070 596
Size: 2085 942 432 353 662 1324
Fragments arranged by size:
2085 1324 942 662 432 353
AflIII A'CryG_T
Cuts at: 942 1204 3150 4950 5231 942
Size: 262 1946 1800 281 1509
Fragments arranged by size:
1946 1800 1509 281 262
AgeI A'CCGG_T
Cuts at: 1366 1366
Size: 5798
AhdI GACnn_n'nnGTC
Cuts at: 1649 1786 1649
Size: 137 5661
AleI CACnn'nnGTG
Cuts at: 683 905 683
Size: 222 5576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2656 2656
Size: 5798
AlwNI CAG_nnn'CTG
Cuts at: 1246 2400 3103 4541 1246
Size: 1154 703 1438 2503
Fragments arranged by size:
2503 1438 1154 703
ApaLI G'TGCA_C
Cuts at: 4636 4636
Size: 5798
ApoI r'AATT_y
Cuts at: 1034 1388 1910 2052 2802 5151 5407 1034
Size: 354 522 142 750 2349 256 1425
Fragments arranged by size:
2349 1425 750 522 354 256 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 5798
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
AvaI C'yCGr_G
Cuts at: 2818 2818
Size: 5798
AvrII C'CTAG_G
Cuts at: 594 594
Size: 5798
BamHI G'GATC_C
Cuts at: 507 1977 2823 507
Size: 1470 846 3482
Fragments arranged by size:
3482 1470 846
BanI G'GyrC_C
Cuts at: 145 859 1491 1842 2065 2640 2814 3228 3476
Size: 714 632 351 223 575 174 414 248
Cuts at: 3476 3511 145
Size: 35 2432
Fragments arranged by size:
2432 714 632 575 414 351 248 223
174 35
BanII G_rGCy'C
Cuts at: 377 2062 2812 3842 377
Size: 1685 750 1030 2333
Fragments arranged by size:
2333 1685 1030 750
BbeI G_GCGC'C
Cuts at: 3232 3480 3232
Size: 248 5550
BbsI GAAGACnn'nnnn_
Cuts at: 268 1369 1433 1645 2186 2495 268
Size: 1101 64 212 541 309 3571
Fragments arranged by size:
3571 1101 541 309 212 64
BbvCI CC'TCA_GC
Cuts at: 1866 1866
Size: 5798
BciVI GTATCCnnnnn_n'
Cuts at: 3696 4752 5798 3696
Size: 1056 1046 3696
Fragments arranged by size:
3696 1056 1046
BclI T'GATC_A
Cuts at: 897 1065 3318 897
Size: 168 2253 3377
Fragments arranged by size:
3377 2253 168
BglI GCCn_nnn'nGGC
Cuts at: 599 599
Size: 5798
BglII A'GATC_T
Cuts at: 3313 5350 3313
Size: 2037 3761
BlpI GC'TnA_GC
Cuts at: 371 2910 371
Size: 2539 3259
Bme1580I G_kGCm'C
Cuts at: 3423 3516 4640 3423
Size: 93 1124 4581
Fragments arranged by size:
4581 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3010 3091 3426 5570 3010
Size: 81 335 2144 3238
Fragments arranged by size:
3238 2144 335 81
BmtI G_CTAG'C
Cuts at: 2543 2543
Size: 5798
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4165 4165
Size: 5798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 590 572
Size: 18 5780
Bpu10I CC'TnA_GC
Cuts at: 1866 1866
Size: 5798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2974 3853 4567 4865 2974
Size: 879 714 298 3907
Fragments arranged by size:
3907 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 590 1636 1794 5502 590
Size: 1046 158 3708 886
Fragments arranged by size:
3708 1046 886 158
BsaAI yAC'GTr
Cuts at: 3151 3151
Size: 5798
BsaBI GATnn'nnATC
Cuts at: 6 3312 5349 6
Size: 3306 2037 455
Fragments arranged by size:
3306 2037 455
BsaHI Gr'CG_yC
Cuts at: 424 623 3229 3477 4179 5675 424
Size: 199 2606 248 702 1496 547
Fragments arranged by size:
2606 1496 702 547 248 199
BsaWI w'CCGG_w
Cuts at: 1366 2987 3508 4186 4597 4744 5018 1366
Size: 1621 521 678 411 147 274 2146
Fragments arranged by size:
2146 1621 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 1774 1804 2232 2262 508
Size: 30 1236 30 428 30 4044
Fragments arranged by size:
4044 1236 428 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 529 751 1854 336
Size: 193 222 1103 4280
Fragments arranged by size:
4280 1103 222 193
BseYI C'CCAG_C
Cuts at: 674 3223 3981 4646 5054 674
Size: 2549 758 665 408 1418
Fragments arranged by size:
2549 1418 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1092 1259 999
Size: 93 167 5538
Fragments arranged by size:
5538 167 93
BsiEI CG_ry'CG
Cuts at: 104 304 317 368 3340 3386 4616 104
Size: 200 13 51 2972 46 1230 1286
Fragments arranged by size:
2972 1286 1230 200 51 46 13
BsiHKAI G_wGCw'C
Cuts at: 2812 3590 3780 4640 2812
Size: 778 190 860 3970
Fragments arranged by size:
3970 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2252 2252
Size: 5798
Bsp1286I G_dGCh'C
Cuts at: 377 2062 2812 3423 3516 3590 3780 3842 4640
Size: 1685 750 611 93 74 190 62 798
Cuts at: 4640 377
Size: 1535
Fragments arranged by size:
1685 1535 798 750 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 1896 4230 1896
Size: 2334 3464
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 1807 2848 3062 3364 3745 941
Size: 866 1041 214 302 381 2994
Fragments arranged by size:
2994 1041 866 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4090 4144 5628 5793 50
Size: 4040 54 1484 165 55
Fragments arranged by size:
4040 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 337 2299 3710 337
Size: 1962 1411 2425
Fragments arranged by size:
2425 1962 1411
BsrFI r'CCGG_y
Cuts at: 148 303 417 1366 3796 3977 148
Size: 155 114 949 2430 181 1969
Fragments arranged by size:
2430 1969 949 181 155 114
BssSI C'ACGA_G
Cuts at: 2000 4069 4777 2000
Size: 2069 708 3021
Fragments arranged by size:
3021 2069 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3175 3175
Size: 5798
BstBI TT'CG_AA
Cuts at: 4159 4159
Size: 5798
BstEII G'GTnAC_C
Cuts at: 285 285
Size: 5798
BstXI CCAn_nnnn'nTGG
Cuts at: 553 553
Size: 5798
BstYI r'GATC_y
Cuts at: 1 507 1977 2332 2550 2823 3290 3313 3648
Size: 506 1470 355 218 273 467 23 335
Cuts at: 3648 3894 4200 4212 4298 5350 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1470 1052 506 467 449 355 335 306
273 246 218 86 23 12
BstZ17I GTA'TAC
Cuts at: 2720 2720
Size: 5798
Bsu36I CC'TnA_GG
Cuts at: 512 2019 512
Size: 1507 4291
BtgI C'CryG_G
Cuts at: 431 503 726 431
Size: 72 223 5503
Fragments arranged by size:
5503 223 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3748 3929 4092 3748
Size: 181 163 5454
Fragments arranged by size:
5454 181 163
BtsI GCAGTG_nn'
Cuts at: 126 3041 126
Size: 2915 2883
DraI TTT'AAA
Cuts at: 2797 2797
Size: 5798
DraIII CAC_nnn'GTG
Cuts at: 1126 1126
Size: 5798
DrdI GACnn_nn'nnGTC
Cuts at: 757 2700 3504 4848 757
Size: 1943 804 1344 1707
Fragments arranged by size:
1943 1707 1344 804
EaeI y'GGCC_r
Cuts at: 301 314 365 2268 3383 3557 3948 3975 5320
Size: 13 51 1903 1115 174 391 27 1345
Cuts at: 5320 301
Size: 779
Fragments arranged by size:
1903 1345 1115 779 391 174 51 27
13
EagI C'GGCC_G
Cuts at: 301 314 365 3383 301
Size: 13 51 3018 2716
Fragments arranged by size:
3018 2716 51 13
EarI CTCTTCn'nnn_
Cuts at: 572 1480 3821 4031 572
Size: 908 2341 210 2339
Fragments arranged by size:
2341 2339 908 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 155 374 4006 4748 4894 5608 155
Size: 219 3632 742 146 714 345
Fragments arranged by size:
3632 742 714 345 219 146
EcoICRI GAG'CTC
Cuts at: 2810 2810
Size: 5798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 590 596 2681 3623 4055 4408 5070 572
Size: 18 6 2085 942 432 353 662 1300
Fragments arranged by size:
2085 1300 942 662 432 353 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 447 2125 447
Size: 1678 4120
EcoO109I rG'GnC_Cy
Cuts at: 452 633 2937 452
Size: 181 2304 3313
Fragments arranged by size:
3313 2304 181
EcoRI G'AATT_C
Cuts at: 1910 2802 1910
Size: 892 4906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 842 3817 842
Size: 2975 2823
FspI TGC'GCA
Cuts at: 3579 3579
Size: 5798
HaeII r_GCGC'y
Cuts at: 3232 3480 4710 5481 3232
Size: 248 1230 771 3549
Fragments arranged by size:
3549 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 508 540 932 964 1635 1667 1772 1804 1933
Size: 32 392 32 671 32 105 32 129
Cuts at: 1933 1965 2403 2425 2435 2457 2511 2538 2543
Size: 32 438 22 10 22 54 27 5
Cuts at: 2543 2570 3640 3670 3672 3702 508
Size: 27 1070 30 2 30 2604
Fragments arranged by size:
2604 1070 671 438 392 129 105 54
32 32 32 32 32 30 30 27
27 22 22 10 5 2
HincII GTy'rAC
Cuts at: 2384 2837 2384
Size: 453 5345
KasI G'GCGC_C
Cuts at: 3228 3476 3228
Size: 248 5550
KpnI G_GTAC'C
Cuts at: 149 2818 149
Size: 2669 3129
MluI A'CGCG_T
Cuts at: 5231 5231
Size: 5798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 825 1976 2148 4557 4741 825
Size: 1151 172 2409 184 1882
Fragments arranged by size:
2409 1882 1151 184 172
MscI TGG'CCA
Cuts at: 3559 3559
Size: 5798
MslI CAynn'nnrTG
Cuts at: 551 653 683 893 905 1059 1143 3787 3914
Size: 102 30 210 12 154 84 2644 127
Cuts at: 3914 551
Size: 2435
Fragments arranged by size:
2644 2435 210 154 127 102 84 30
12
MspA1I CmG'CkG
Cuts at: 215 370 433 505 2909 3223 3583 4367 4612
Size: 155 63 72 2404 314 360 784 245
Cuts at: 4612 215
Size: 1401
Fragments arranged by size:
2404 1401 784 360 314 245 155 72
63
NaeI GCC'GGC
Cuts at: 305 419 3979 305
Size: 114 3560 2124
Fragments arranged by size:
3560 2124 114
NarI GG'CG_CC
Cuts at: 3229 3477 3229
Size: 248 5550
NcoI C'CATG_G
Cuts at: 726 726
Size: 5798
NdeI CA'TA_TG
Cuts at: 1100 1100
Size: 5798
NgoMIV G'CCGG_C
Cuts at: 303 417 3977 303
Size: 114 3560 2124
Fragments arranged by size:
3560 2124 114
NheI G'CTAG_C
Cuts at: 2539 2539
Size: 5798
NspI r_CATG'y
Cuts at: 658 946 1208 1410 2851 3351 4954 658
Size: 288 262 202 1441 500 1603 1502
Fragments arranged by size:
1603 1502 1441 500 288 262 202
PasI CC'CwG_GG
Cuts at: 249 249
Size: 5798
PciI A'CATG_T
Cuts at: 942 1204 4950 942
Size: 262 3746 1790
Fragments arranged by size:
3746 1790 262
PflMI CCAn_nnn'nTGG
Cuts at: 255 2400 255
Size: 2145 3653
PfoI T'CCnGG_A
Cuts at: 629 629
Size: 5798
PmeI GTTT'AAAC
Cuts at: 2797 2797
Size: 5798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 5766
PpuMI rG'GwC_Cy
Cuts at: 452 633 452
Size: 181 5617
PstI C_TGCA'G
Cuts at: 1484 1818 2845 3530 1484
Size: 334 1027 685 3752
Fragments arranged by size:
3752 1027 685 334
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5798
PvuII CAG'CTG
Cuts at: 3223 3583 3223
Size: 360 5438
RsrII CG'GwC_CG
Cuts at: 3993 3993
Size: 5798
SacI G_AGCT'C
Cuts at: 2812 2812
Size: 5798
SacII CC_GC'GG
Cuts at: 434 506 434
Size: 72 5726
SalI G'TCGA_C
Cuts at: 2835 2835
Size: 5798
SapI GCTCTTCn'nnn_
Cuts at: 3821 4031 3821
Size: 210 5588
SbfI CC_TGCA'GG
Cuts at: 2845 2845
Size: 5798
ScaI AGT'ACT
Cuts at: 5315 5315
Size: 5798
SfcI C'TryA_G
Cuts at: 33 903 1018 1480 1814 2841 3526 4494 4685
Size: 870 115 462 334 1027 685 968 191
Cuts at: 4685 33
Size: 1146
Fragments arranged by size:
1146 1027 968 870 685 462 334 191
115
SfoI GGC'GCC
Cuts at: 3230 3478 3230
Size: 248 5550
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
SmaI CCC'GGG
Cuts at: 2820 2820
Size: 5798
SmlI C'TyrA_G
Cuts at: 2953 3868 4582 4844 2953
Size: 915 714 262 3907
Fragments arranged by size:
3907 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1234 67
Size: 1167 4631
SphI G_CATG'C
Cuts at: 2851 3351 2851
Size: 500 5298
SspI AAT'ATT
Cuts at: 1989 1989
Size: 5798
StuI AGG'CCT
Cuts at: 593 593
Size: 5798
StyI C'CwwG_G
Cuts at: 594 726 2932 3286 594
Size: 132 2206 354 3106
Fragments arranged by size:
3106 2206 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2083 2787 3381 3514 3973 4182 2083
Size: 704 594 133 459 209 3699
Fragments arranged by size:
3699 704 594 459 209 133
TatI w'GTAC_w
Cuts at: 1663 2755 5313 1663
Size: 1092 2558 2148
Fragments arranged by size:
2558 2148 1092
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 804 836 1110 1142 1774 1806 2205 2232 2237
Size: 32 274 32 632 32 399 27 5
Cuts at: 2237 2264 5315 5347 804
Size: 27 3051 32 1255
Fragments arranged by size:
3051 1255 632 399 274 32 32 32
32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 3595 3595
Size: 5798
XbaI T'CTAG_A
Cuts at: 2829 2829
Size: 5798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 550 837 1666 3293 550
Size: 287 829 1627 3055
Fragments arranged by size:
3055 1627 829 287
XmaI C'CCGG_G
Cuts at: 2818 2818
Size: 5798
XmnI GAAnn'nnTTC
Cuts at: 2039 2039
Size: 5798
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI BaeI BcgI
BfrBI BmgBI BsiWI BsmI BspEI BsrGI BssHII ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI