Restriction map for
pF1KB3321
(Circular) (Six-base) MAPSORT of: pF1KB3321.seq Check: 673 from: 1 to: 5882
pF1KB3321 5882 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 448 1893 1918 132
Size: 316 1445 25 4096
Fragments arranged by size:
4096 1445 316 25
AccI GT'mk_AC
Cuts at: 1264 2504 2920 1264
Size: 1240 416 4226
Fragments arranged by size:
4226 1240 416
Acc65I G'GTAC_C
Cuts at: 2898 2898
Size: 5882
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2842 3707 4139 4492 5154 2842
Size: 865 432 353 662 3570
Fragments arranged by size:
3570 865 662 432 353
AfeI AGC'GCT
Cuts at: 2224 2224
Size: 5882
AflIII A'CryG_T
Cuts at: 3234 5034 5315 3234
Size: 1800 281 3801
Fragments arranged by size:
3801 1800 281
AgeI A'CCGG_T
Cuts at: 653 1259 653
Size: 606 5276
AhdI GACnn_n'nnGTC
Cuts at: 541 541
Size: 5882
AleI CACnn'nnGTG
Cuts at: 2602 2602
Size: 5882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1210 1970 1210
Size: 760 5122
AlwNI CAG_nnn'CTG
Cuts at: 282 318 582 1760 1908 2193 2775 2801 2820
Size: 36 264 1178 148 285 582 26 19
Cuts at: 2820 3187 4625 282
Size: 367 1438 1539
Fragments arranged by size:
1539 1438 1178 582 367 285 264 148
36 26 19
ApaI G_GGCC'C
Cuts at: 668 2057 2185 2304 668
Size: 1389 128 119 4246
Fragments arranged by size:
4246 1389 128 119
ApaLI G'TGCA_C
Cuts at: 4720 4720
Size: 5882
ApoI r'AATT_y
Cuts at: 2886 5235 5491 2886
Size: 2349 256 3277
Fragments arranged by size:
3277 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5882
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 661 667 673 1067 2297 2467 2861 2902 661
Size: 6 6 394 1230 170 394 41 3641
Fragments arranged by size:
3641 1230 394 394 170 41 6 6
BamHI G'GATC_C
Cuts at: 2481 2907 2481
Size: 426 5456
BanI G'GyrC_C
Cuts at: 210 595 1222 1279 2898 3312 3560 3595 210
Size: 385 627 57 1619 414 248 35 2497
Fragments arranged by size:
2497 1619 627 414 385 248 57 35
BanII G_rGCy'C
Cuts at: 198 668 674 680 1745 1985 2057 2068 2185
Size: 470 6 6 1065 240 72 11 117
Cuts at: 2185 2304 2896 3926 198
Size: 119 592 1030 2154
Fragments arranged by size:
2154 1065 1030 592 470 240 119 117
72 11 6 6
BbeI G_GCGC'C
Cuts at: 1226 3316 3564 1226
Size: 2090 248 3544
Fragments arranged by size:
3544 2090 248
BbsI GAAGACnn'nnnn_
Cuts at: 1100 1380 1100
Size: 280 5602
BbvCI CC'TCA_GC
Cuts at: 1360 1627 2072 2389 1360
Size: 267 445 317 4853
Fragments arranged by size:
4853 445 317 267
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 437 403
Size: 34 5848
BciVI GTATCCnnnnn_n'
Cuts at: 2158 3780 4836 5882 2158
Size: 1622 1056 1046 2158
Fragments arranged by size:
2158 1622 1056 1046
BclI T'GATC_A
Cuts at: 3402 3402
Size: 5882
BfrBI ATG'CAT
Cuts at: 1687 1687
Size: 5882
BglI GCCn_nnn'nGGC
Cuts at: 1647 1722 1647
Size: 75 5807
BglII A'GATC_T
Cuts at: 1499 3397 5434 1499
Size: 1898 2037 1947
Fragments arranged by size:
2037 1947 1898
BlpI GC'TnA_GC
Cuts at: 2994 2994
Size: 5882
Bme1580I G_kGCm'C
Cuts at: 600 668 949 2057 2185 2291 2304 3507 3600
Size: 68 281 1108 128 106 13 1203 93
Cuts at: 3600 4724 600
Size: 1124 1758
Fragments arranged by size:
1758 1203 1124 1108 281 128 106 93
68 13
BmgBI CAC'GTC
Cuts at: 207 207
Size: 5882
BmrI ACTGGGnnnn_n'
Cuts at: 630 2265 3094 3175 3510 5654 630
Size: 1635 829 81 335 2144 858
Fragments arranged by size:
2144 1635 858 829 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 399 1230 1232 4249 399
Size: 831 2 3017 2032
Fragments arranged by size:
3017 2032 831 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 394 1225 1561 2151 2239 2890 307
Size: 87 831 336 590 88 651 3299
Fragments arranged by size:
3299 831 651 590 336 88 87
Bpu10I CC'TnA_GC
Cuts at: 400 1360 1627 2072 2311 2389 2776 400
Size: 960 267 445 239 78 387 3506
Fragments arranged by size:
3506 960 445 387 267 239 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2326 3058 3937 4651 4949 2326
Size: 732 879 714 298 3259
Fragments arranged by size:
3259 879 732 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 452 5586 452
Size: 5134 748
BsaAI yAC'GTr
Cuts at: 3235 3235
Size: 5882
BsaBI GATnn'nnATC
Cuts at: 6 3396 5433 6
Size: 3390 2037 455
Fragments arranged by size:
3390 2037 455
BsaHI Gr'CG_yC
Cuts at: 749 1223 3313 3561 4263 5759 749
Size: 474 2090 248 702 1496 872
Fragments arranged by size:
2090 1496 872 702 474 248
BsaWI w'CCGG_w
Cuts at: 461 653 1259 2447 3071 3592 4270 4681 4828
Size: 192 606 1188 624 521 678 411 147
Cuts at: 4828 5102 461
Size: 274 1241
Fragments arranged by size:
1241 1188 678 624 606 521 411 274
192 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 691 721 691
Size: 30 5852
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 361 715 718 835 1682 361
Size: 354 3 117 847 4561
Fragments arranged by size:
4561 847 354 117 3
BseYI C'CCAG_C
Cuts at: 168 406 734 1396 2049 2178 2186 2303 3307
Size: 238 328 662 653 129 8 117 1004
Cuts at: 3307 4065 4730 5138 168
Size: 758 665 408 912
Fragments arranged by size:
1004 912 758 665 662 653 408 328
238 129 117 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1408 2647 814
Size: 594 1239 4049
Fragments arranged by size:
4049 1239 594
BsiEI CG_ry'CG
Cuts at: 104 3424 3470 4700 104
Size: 3320 46 1230 1286
Fragments arranged by size:
3320 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 183 293 401 1338 2782 2896 3674 3864 4724
Size: 110 108 937 1444 114 778 190 860
Cuts at: 4724 183
Size: 1341
Fragments arranged by size:
1444 1341 937 860 778 190 114 110
108
BsmI GAATG_Cn'
Cuts at: 1689 1689
Size: 5882
BsmBI CGTCTCn'nnnn_
Cuts at: 1999 1999
Size: 5882
Bsp1286I G_dGCh'C
Cuts at: 183 198 293 401 600 668 674 680 949
Size: 15 95 108 199 68 6 6 269
Cuts at: 949 1338 1745 1985 2057 2068 2185 2291 2304
Size: 389 407 240 72 11 117 106 13
Cuts at: 2304 2782 2896 3507 3600 3674 3864 3926 4724
Size: 478 114 611 93 74 190 62 798
Cuts at: 4724 183
Size: 1341
Fragments arranged by size:
1341 798 611 478 407 389 269 240
199 190 117 114 108 106 95 93
74 72 68 62 15 13 11 6
6
BspEI T'CCGG_A
Cuts at: 2447 2447
Size: 5882
BspHI T'CATG_A
Cuts at: 690 1338 2583 4314 690
Size: 648 1245 1731 2258
Fragments arranged by size:
2258 1731 1245 648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 448 1631 1893 1918 2932 3146 3448 3829
Size: 316 1183 262 25 1014 214 302 381
Cuts at: 3829 132
Size: 2185
Fragments arranged by size:
2185 1183 1014 381 316 302 262 214
25
BsrBI CCG'CTC
Cuts at: 50 393 2430 4174 4228 5712 5877 50
Size: 343 2037 1744 54 1484 165 55
Fragments arranged by size:
2037 1744 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3794 3794
Size: 5882
BsrFI r'CCGG_y
Cuts at: 254 653 1225 1259 1802 3880 4061 254
Size: 399 572 34 543 2078 181 2075
Fragments arranged by size:
2078 2075 572 543 399 181 34
BssSI C'ACGA_G
Cuts at: 2728 4153 4861 2728
Size: 1425 708 3749
Fragments arranged by size:
3749 1425 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1503 1933 3259 1503
Size: 430 1326 4126
Fragments arranged by size:
4126 1326 430
BstBI TT'CG_AA
Cuts at: 771 4243 771
Size: 3472 2410
BstEII G'GTnAC_C
Cuts at: 987 1008 987
Size: 21 5861
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1173 1885 1977 2103 114
Size: 1059 712 92 126 3893
Fragments arranged by size:
3893 1059 712 126 92
BstYI r'GATC_y
Cuts at: 1 521 973 1292 1499 2481 2907 3374 3397
Size: 520 452 319 207 982 426 467 23
Cuts at: 3397 3732 3978 4284 4296 4382 5434 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 982 520 467 452 449 426 335
319 306 246 207 86 23 12
BstZ17I GTA'TAC
Cuts at: 1265 2505 1265
Size: 1240 4642
Bsu36I CC'TnA_GG
Cuts at: 475 475
Size: 5882
BtgI C'CryG_G
Cuts at: 184 680 993 1316 1959 184
Size: 496 313 323 643 4107
Fragments arranged by size:
4107 643 496 323 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1668 3832 4013 4176 1668
Size: 2164 181 163 3374
Fragments arranged by size:
3374 2164 181 163
BtsI GCAGTG_nn'
Cuts at: 496 1642 2667 3125 496
Size: 1146 1025 458 3253
Fragments arranged by size:
3253 1146 1025 458
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DraIII CAC_nnn'GTG
Cuts at: 953 1818 2157 2175 953
Size: 865 339 18 4660
Fragments arranged by size:
4660 865 339 18
DrdI GACnn_nn'nnGTC
Cuts at: 3588 4932 3588
Size: 1344 4538
EaeI y'GGCC_r
Cuts at: 121 434 501 710 1562 1639 1804 1967 2261
Size: 313 67 209 852 77 165 163 294
Cuts at: 2261 2399 2642 3467 3641 4032 4059 5404 121
Size: 138 243 825 174 391 27 1345 599
Fragments arranged by size:
1345 852 825 599 391 313 294 243
209 174 165 163 138 77 67 27
EagI C'GGCC_G
Cuts at: 3467 3467
Size: 5882
EarI CTCTTCn'nnn_
Cuts at: 794 2143 3905 4115 794
Size: 1349 1762 210 2561
Fragments arranged by size:
2561 1762 1349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2451 4090 4832 4978 5692 2451
Size: 1639 742 146 714 2641
Fragments arranged by size:
2641 1639 742 714 146
EcoICRI GAG'CTC
Cuts at: 2894 2894
Size: 5882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 394 1225 1561 2151 2239 2842 2890 3707
Size: 87 831 336 590 88 603 48 817
Cuts at: 3707 4139 4492 5154 307
Size: 432 353 662 1035
Fragments arranged by size:
1035 831 817 662 603 590 432 353
336 88 87 48
EcoNI CCTnn'n_nnAGG
Cuts at: 376 1021 2728 376
Size: 645 1707 3530
Fragments arranged by size:
3530 1707 645
EcoO109I rG'GnC_Cy
Cuts at: 742 2053 2100 2182 3021 742
Size: 1311 47 82 839 3603
Fragments arranged by size:
3603 1311 839 82 47
EcoRI G'AATT_C
Cuts at: 2886 2886
Size: 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1146 3901 1146
Size: 2755 3127
FspI TGC'GCA
Cuts at: 3663 3663
Size: 5882
HaeII r_GCGC'y
Cuts at: 1226 1403 2226 3316 3564 4794 5565 1226
Size: 177 823 1090 248 1230 771 1543
Fragments arranged by size:
1543 1230 1090 823 771 248 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 527 559 2501 2533 3724 3754 3756 3786 527
Size: 32 1942 32 1191 30 2 30 2623
Fragments arranged by size:
2623 1942 1191 32 32 30 30 2
HincII GTy'rAC
Cuts at: 981 1823 2007 2921 981
Size: 842 184 914 3942
Fragments arranged by size:
3942 914 842 184
KasI G'GCGC_C
Cuts at: 1222 3312 3560 1222
Size: 2090 248 3544
Fragments arranged by size:
3544 2090 248
KpnI G_GTAC'C
Cuts at: 2902 2902
Size: 5882
MluI A'CGCG_T
Cuts at: 5315 5315
Size: 5882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 1289 1646 4641 4825 881
Size: 408 357 2995 184 1938
Fragments arranged by size:
2995 1938 408 357 184
MscI TGG'CCA
Cuts at: 123 436 1564 1641 1969 2263 2644 3643 123
Size: 313 1128 77 328 294 381 999 2362
Fragments arranged by size:
2362 1128 999 381 328 313 294 77
MslI CAynn'nnrTG
Cuts at: 112 1433 2602 3871 3998 112
Size: 1321 1169 1269 127 1996
Fragments arranged by size:
1996 1321 1269 1169 127
MspA1I CmG'CkG
Cuts at: 186 279 417 738 1044 1760 1833 2049 2115
Size: 93 138 321 306 716 73 216 66
Cuts at: 2115 2307 2820 2841 2993 3307 3667 4451 4696
Size: 192 513 21 152 314 360 784 245
Cuts at: 4696 186
Size: 1372
Fragments arranged by size:
1372 784 716 513 360 321 314 306
245 216 192 152 138 93 73 66
21
NaeI GCC'GGC
Cuts at: 1227 1804 4063 1227
Size: 577 2259 3046
Fragments arranged by size:
3046 2259 577
NarI GG'CG_CC
Cuts at: 1223 3313 3561 1223
Size: 2090 248 3544
Fragments arranged by size:
3544 2090 248
NcoI C'CATG_G
Cuts at: 993 1316 993
Size: 323 5559
NgoMIV G'CCGG_C
Cuts at: 1225 1802 4061 1225
Size: 577 2259 3046
Fragments arranged by size:
3046 2259 577
NsiI A_TGCA'T
Cuts at: 1689 1689
Size: 5882
NspI r_CATG'y
Cuts at: 2762 2935 3435 5038 2762
Size: 173 500 1603 3606
Fragments arranged by size:
3606 1603 500 173
PasI CC'CwG_GG
Cuts at: 531 531
Size: 5882
PciI A'CATG_T
Cuts at: 5034 5034
Size: 5882
PflMI CCAn_nnn'nTGG
Cuts at: 564 1524 1665 2157 2801 564
Size: 960 141 492 644 3645
Fragments arranged by size:
3645 960 644 492 141
PfoI T'CCnGG_A
Cuts at: 550 629 550
Size: 79 5803
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 4328
Size: 32 5850
PpuMI rG'GwC_Cy
Cuts at: 2100 2100
Size: 5882
PshAI GACnn'nnGTC
Cuts at: 1100 1100
Size: 5882
PspOMI G'GGCC_C
Cuts at: 664 2053 2181 2300 664
Size: 1389 128 119 4246
Fragments arranged by size:
4246 1389 128 119
PstI C_TGCA'G
Cuts at: 1832 1929 1949 2252 2929 3614 1832
Size: 97 20 303 677 685 4100
Fragments arranged by size:
4100 685 677 303 97 20
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5882
PvuII CAG'CTG
Cuts at: 279 417 1044 1760 1833 2049 2115 2307 2820
Size: 138 627 716 73 216 66 192 513
Cuts at: 2820 3307 3667 279
Size: 487 360 2494
Fragments arranged by size:
2494 716 627 513 487 360 216 192
138 73 66
RsrII CG'GwC_CG
Cuts at: 4077 4077
Size: 5882
SacI G_AGCT'C
Cuts at: 2896 2896
Size: 5882
SacII CC_GC'GG
Cuts at: 187 187
Size: 5882
SalI G'TCGA_C
Cuts at: 2919 2919
Size: 5882
SapI GCTCTTCn'nnn_
Cuts at: 794 3905 4115 794
Size: 3111 210 2561
Fragments arranged by size:
3111 2561 210
SbfI CC_TGCA'GG
Cuts at: 2929 2929
Size: 5882
ScaI AGT'ACT
Cuts at: 2254 5399 2254
Size: 3145 2737
SexAI A'CCwGG_T
Cuts at: 1592 2084 2153 1592
Size: 492 69 5321
Fragments arranged by size:
5321 492 69
SfcI C'TryA_G
Cuts at: 33 1828 1925 1945 2248 2925 3610 4578 4769
Size: 1795 97 20 303 677 685 968 191
Cuts at: 4769 33
Size: 1146
Fragments arranged by size:
1795 1146 968 685 677 303 191 97
20
SfoI GGC'GCC
Cuts at: 1224 3314 3562 1224
Size: 2090 248 3544
Fragments arranged by size:
3544 2090 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SmaI CCC'GGG
Cuts at: 663 1069 2299 2904 663
Size: 406 1230 605 3641
Fragments arranged by size:
3641 1230 605 406
SmlI C'TyrA_G
Cuts at: 2341 2467 3037 3952 4666 4928 2341
Size: 126 570 915 714 262 3295
Fragments arranged by size:
3295 915 714 570 262 126
SpeI A'CTAG_T
Cuts at: 67 2601 67
Size: 2534 3348
SphI G_CATG'C
Cuts at: 2935 3435 2935
Size: 500 5382
StuI AGG'CCT
Cuts at: 382 382
Size: 5882
StyI C'CwwG_G
Cuts at: 993 1316 1895 2096 3016 3370 993
Size: 323 579 201 920 354 3505
Fragments arranged by size:
3505 920 579 354 323 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 650 1512 2247 3465 3598 4057 4266 650
Size: 862 735 1218 133 459 209 2266
Fragments arranged by size:
2266 1218 862 735 459 209 133
TatI w'GTAC_w
Cuts at: 2252 2456 5397 2252
Size: 204 2941 2737
Fragments arranged by size:
2941 2737 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 5399 5431 2772
Size: 32 2595 32 3223
Fragments arranged by size:
3223 2595 32 32
Tth111I GACn'n_nGTC
Cuts at: 3679 3679
Size: 5882
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2214 2798 3377 2214
Size: 584 579 4719
Fragments arranged by size:
4719 584 579
XhoI C'TCGA_G
Cuts at: 2467 2467
Size: 5882
XmaI C'CCGG_G
Cuts at: 661 1067 2297 2902 661
Size: 406 1230 605 3641
Fragments arranged by size:
3641 1230 605 406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BmtI BsiWI
BsrGI BssHII ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI