Restriction map for
pF1KB3277
(Circular) (Six-base) MAPSORT of: pF1KB3277.seq Check: 9312 from: 1 to: 4928
pF1KB3277 4928 bp
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AccI GT'mk_AC
Cuts at: 1966 1966
Size: 4928
Acc65I G'GTAC_C
Cuts at: 774 1944 774
Size: 1170 3758
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1511 2753 3185 3932 4200 835
Size: 676 1242 432 747 268 1563
Fragments arranged by size:
1563 1242 747 676 432 268
AfeI AGC'GCT
Cuts at: 851 851
Size: 4928
AflIII A'CryG_T
Cuts at: 2280 3384 4361 2280
Size: 1104 977 2847
Fragments arranged by size:
2847 1104 977
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AhdI GACnn_n'nnGTC
Cuts at: 793 793
Size: 4928
AlwNI CAG_nnn'CTG
Cuts at: 349 2233 3800 349
Size: 1884 1567 1477
Fragments arranged by size:
1884 1567 1477
ApaI G_GGCC'C
Cuts at: 808 808
Size: 4928
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 400 523 549 1932 4281 4537 400
Size: 123 26 1383 2349 256 791
Fragments arranged by size:
2349 1383 791 256 123 26
AseI AT'TA_AT
Cuts at: 20 1076 20
Size: 1056 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 1172 1769 1948 1172
Size: 597 179 4152
Fragments arranged by size:
4152 597 179
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 774 1760 1944 2358 2606 2641 774
Size: 986 184 414 248 35 3061
Fragments arranged by size:
3061 986 414 248 184 35
BanII G_rGCy'C
Cuts at: 808 1942 2972 808
Size: 1134 1030 2764
Fragments arranged by size:
2764 1134 1030
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4680
BbsI GAAGACnn'nnnn_
Cuts at: 751 751
Size: 4928
BbvCI CC'TCA_GC
Cuts at: 1545 1545
Size: 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4894
BciVI GTATCCnnnnn_n'
Cuts at: 1062 2826 3587 4928 1062
Size: 1764 761 1341 1062
Fragments arranged by size:
1764 1341 1062 761
BclI T'GATC_A
Cuts at: 1443 2448 1443
Size: 1005 3923
BglII A'GATC_T
Cuts at: 2443 4480 2443
Size: 2037 2891
BlpI GC'TnA_GC
Cuts at: 993 2040 993
Size: 1047 3881
Bme1580I G_kGCm'C
Cuts at: 808 2553 2646 3702 808
Size: 1745 93 1056 2034
Fragments arranged by size:
2034 1745 1056 93
BmgBI CAC'GTC
Cuts at: 1776 1776
Size: 4928
BmrI ACTGGGnnnn_n'
Cuts at: 463 680 944 2140 2221 2556 4700 463
Size: 217 264 1196 81 335 2144 691
Fragments arranged by size:
2144 1196 691 335 264 217 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 516 1226 1333 1575 226
Size: 290 710 107 242 3579
Fragments arranged by size:
3579 710 290 242 107
Bpu10I CC'TnA_GC
Cuts at: 1369 1545 1369
Size: 176 4752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1169 1300 1421 2104 2983 3475 3773 1169
Size: 131 121 683 879 492 298 2324
Fragments arranged by size:
2324 879 683 492 298 131 121
BsaI GGTCTCn'nnnn_
Cuts at: 1714 4632 1714
Size: 2918 2010
BsaAI yAC'GTr
Cuts at: 1644 1677 2281 1644
Size: 33 604 4291
Fragments arranged by size:
4291 604 33
BsaBI GATnn'nnATC
Cuts at: 6 892 2442 4479 6
Size: 886 1550 2037 455
Fragments arranged by size:
2037 1550 886 455
BsaHI Gr'CG_yC
Cuts at: 2359 2607 3309 4805 2359
Size: 248 702 1496 2482
Fragments arranged by size:
2482 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2117 2638 3316 3590 3737 4148 2117
Size: 521 678 274 147 411 2897
Fragments arranged by size:
2897 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 1323 1353 658
Size: 30 635 30 4233
Fragments arranged by size:
4233 635 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 749 1533 223
Size: 526 784 3618
Fragments arranged by size:
3618 784 526
BseYI C'CCAG_C
Cuts at: 1342 1526 1888 2353 3111 3688 4184 1342
Size: 184 362 465 758 577 496 2086
Fragments arranged by size:
2086 758 577 496 465 362 184
BsiEI CG_ry'CG
Cuts at: 104 2470 2516 3724 104
Size: 2366 46 1208 1308
Fragments arranged by size:
2366 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 755 1298 1942 2720 2910 3702 755
Size: 543 644 778 190 792 1981
Fragments arranged by size:
1981 792 778 644 543 190
BsmI GAATG_Cn'
Cuts at: 734 734
Size: 4928
BsmBI CGTCTCn'nnnn_
Cuts at: 1709 1709
Size: 4928
Bsp1286I G_dGCh'C
Cuts at: 755 808 1298 1942 2553 2646 2720 2910 2972
Size: 53 490 644 611 93 74 190 62
Cuts at: 2972 3702 755
Size: 730 1981
Fragments arranged by size:
1981 730 644 611 490 190 93 74
62 53
BspEI T'CCGG_A
Cuts at: 4148 4148
Size: 4928
BspHI T'CATG_A
Cuts at: 4104 4104
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1978 2192 2494 2875 1978
Size: 214 302 381 4031
Fragments arranged by size:
4031 381 302 214
BsrBI CCG'CTC
Cuts at: 50 243 674 1465 1535 3220 3274 4758 4923
Size: 193 431 791 70 1685 54 1484 165
Cuts at: 4923 50
Size: 55
Fragments arranged by size:
1685 1484 791 431 193 165 70 55
54
BsrDI GCAATG_nn'
Cuts at: 1042 2840 1042
Size: 1798 3130
BsrFI r'CCGG_y
Cuts at: 134 2926 3107 3316 134
Size: 2792 181 209 1746
Fragments arranged by size:
2792 1746 209 181
BssSI C'ACGA_G
Cuts at: 3199 3557 3199
Size: 358 4570
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2305
Size: 4928
BstBI TT'CG_AA
Cuts at: 368 3289 368
Size: 2921 2007
BstEII G'GTnAC_C
Cuts at: 903 903
Size: 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 1570 1570
Size: 4928
BstYI r'GATC_y
Cuts at: 1 973 1953 2420 2443 2778 3024 4036 4122
Size: 972 980 467 23 335 246 1012 86
Cuts at: 4122 4134 4480 1
Size: 12 346 449
Fragments arranged by size:
1012 980 972 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1145 1145
Size: 4928
BtgI C'CryG_G
Cuts at: 1725 1725
Size: 4928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 115 1549 2878 3059 3222 115
Size: 1434 1329 181 163 1821
Fragments arranged by size:
1821 1434 1329 181 163
BtsI GCAGTG_nn'
Cuts at: 118 230 1575 1740 2171 118
Size: 112 1345 165 431 2875
Fragments arranged by size:
2875 1345 431 165 112
DraI TTT'AAA
Cuts at: 866 1927 866
Size: 1061 3867
DraIII CAC_nnn'GTG
Cuts at: 1699 1699
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3492 2634
Size: 858 4070
EaeI y'GGCC_r
Cuts at: 356 2513 2687 3078 3105 4450 356
Size: 2157 174 391 27 1345 834
Fragments arranged by size:
2157 1345 834 391 174 27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 1542 2951 3161 1542
Size: 1409 210 3309
Fragments arranged by size:
3309 1409 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 900 1906 3136 3446 3592 4738 900
Size: 1006 1230 310 146 1146 1090
Fragments arranged by size:
1230 1146 1090 1006 310 146
EcoICRI GAG'CTC
Cuts at: 1940 1940
Size: 4928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 516 835 1226 1333 1511 1575 2753 3185
Size: 290 319 391 107 178 64 1178 432
Cuts at: 3185 3932 4200 226
Size: 747 268 954
Fragments arranged by size:
1178 954 747 432 391 319 290 268
178 107 64
EcoO109I rG'GnC_Cy
Cuts at: 805 2067 805
Size: 1262 3666
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4928
EcoRV GAT'ATC
Cuts at: 890 1868 890
Size: 978 3950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4928
FspI TGC'GCA
Cuts at: 229 2709 229
Size: 2480 2448
HaeII r_GCGC'y
Cuts at: 853 2362 2610 3632 4611 853
Size: 1509 248 1022 979 1170
Fragments arranged by size:
1509 1170 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 779 811 1125 1157 1440 1472 2770 2800 2802
Size: 32 314 32 283 32 1298 30 2
Cuts at: 2802 2832 779
Size: 30 2875
Fragments arranged by size:
2875 1298 314 283 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1967 1967
Size: 4928
HindIII A'AGCT_T
Cuts at: 990 990
Size: 4928
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4680
KpnI G_GTAC'C
Cuts at: 778 1948 778
Size: 1170 3758
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 1001 3599 3783 242
Size: 759 2598 184 1387
Fragments arranged by size:
2598 1387 759 184
MscI TGG'CCA
Cuts at: 358 2689 358
Size: 2331 2597
MslI CAynn'nnrTG
Cuts at: 332 835 1568 2917 3044 332
Size: 503 733 1349 127 2216
Fragments arranged by size:
2216 1349 733 503 127
MspA1I CmG'CkG
Cuts at: 910 1219 2039 2353 2713 3726 3971 910
Size: 309 820 314 360 1013 245 1867
Fragments arranged by size:
1867 1013 820 360 314 309 245
NaeI GCC'GGC
Cuts at: 136 3109 136
Size: 2973 1955
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4680
NgoMIV G'CCGG_C
Cuts at: 134 3107 134
Size: 2973 1955
NspI r_CATG'y
Cuts at: 391 1981 2481 3388 391
Size: 1590 500 907 1931
Fragments arranged by size:
1931 1590 907 500
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4928
PflMI CCAn_nnn'nTGG
Cuts at: 349 349
Size: 4928
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PmlI CAC'GTG
Cuts at: 1677 1677
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4896
PshAI GACnn'nnGTC
Cuts at: 307 307
Size: 4928
PspOMI G'GGCC_C
Cuts at: 804 804
Size: 4928
PstI C_TGCA'G
Cuts at: 497 1975 2660 497
Size: 1478 685 2765
Fragments arranged by size:
2765 1478 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 1219 2353 2713 1219
Size: 1134 360 3434
Fragments arranged by size:
3434 1134 360
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1942 1942
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 1542 2951 3161 1542
Size: 1409 210 3309
Fragments arranged by size:
3309 1409 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SfcI C'TryA_G
Cuts at: 33 315 493 614 1971 2656 3649 3840 33
Size: 282 178 121 1357 685 993 191 1121
Fragments arranged by size:
1357 1121 993 685 282 191 178 121
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4680
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SgrAI Cr'CCGG_yG
Cuts at: 134 134
Size: 4928
SmaI CCC'GGG
Cuts at: 1771 1950 1771
Size: 179 4749
SmlI C'TyrA_G
Cuts at: 1172 1184 1279 1436 2083 2998 3490 3752 1172
Size: 12 95 157 647 915 492 262 2348
Fragments arranged by size:
2348 915 647 492 262 157 95 12
SnaBI TAC'GTA
Cuts at: 1644 1644
Size: 4928
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4428
StuI AGG'CCT
Cuts at: 1318 1318
Size: 4928
StyI C'CwwG_G
Cuts at: 1106 1832 2062 2416 1106
Size: 726 230 354 3618
Fragments arranged by size:
3618 726 354 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 556 1348 2511 2644 3103 3312 556
Size: 792 1163 133 459 209 2172
Fragments arranged by size:
2172 1163 792 459 209 133
TatI w'GTAC_w
Cuts at: 4443 4443
Size: 4928
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 1323 1355 1522 1554 1738 1770 4445
Size: 32 1126 32 167 32 184 32 2675
Cuts at: 4445 4477 165
Size: 32 616
Fragments arranged by size:
2675 1126 616 184 167 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 2423 789
Size: 1634 3294
XhoI C'TCGA_G
Cuts at: 1172 1172
Size: 4928
XmaI C'CCGG_G
Cuts at: 1769 1948 1769
Size: 179 4749
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmtI BsgI BsiWI BsrGI BssHII
BstZ17I ClaI CspCI EcoNI FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SrfI SspI SwaI XmnI ZraI