Restriction map for
pF1KB3179
(Circular) (Six-base) MAPSORT of: pF1KB3179.seq Check: 8412 from: 1 to: 7709
pF1KB3179 7709 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3297 3297
Size: 7709
AccI GT'mk_AC
Cuts at: 247 682 758 3435 4747 247
Size: 435 76 2677 1312 3209
Fragments arranged by size:
3209 2677 1312 435 76
Acc65I G'GTAC_C
Cuts at: 3493 4725 3493
Size: 1232 6477
AclI AA'CG_TT
Cuts at: 7549 7549
Size: 7709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 478 2144 2174 3074 3112 4127 5534 5966
Size: 319 1666 30 900 38 1015 1407 432
Cuts at: 5966 6713 6981 159
Size: 747 268 887
Fragments arranged by size:
1666 1407 1015 900 887 747 432 319
268 38 30
AflIII A'CryG_T
Cuts at: 447 1721 5061 6165 7142 447
Size: 1274 3340 1104 977 1014
Fragments arranged by size:
3340 1274 1104 1014 977
AgeI A'CCGG_T
Cuts at: 6097 6097
Size: 7709
AleI CACnn'nnGTG
Cuts at: 4181 4181
Size: 7709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1317 2410 1317
Size: 1093 6616
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1904 1936 1904
Size: 32 7677
AlwNI CAG_nnn'CTG
Cuts at: 5014 6581 5014
Size: 1567 6142
ApaI G_GGCC'C
Cuts at: 2591 4519 2591
Size: 1928 5781
ApaLI G'TGCA_C
Cuts at: 6479 6479
Size: 7709
ApoI r'AATT_y
Cuts at: 131 289 825 951 1006 1251 1740 2487 2614
Size: 158 536 126 55 245 489 747 127
Cuts at: 2614 2690 2774 3262 3536 4385 4713 7062 7318
Size: 76 84 488 274 849 328 2349 256
Cuts at: 7318 131
Size: 522
Fragments arranged by size:
2349 849 747 536 522 489 488 328
274 256 245 158 127 126 84 76
55
AseI AT'TA_AT
Cuts at: 20 3139 20
Size: 3119 4590
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7709
AvaI C'yCGr_G
Cuts at: 4729 4729
Size: 7709
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3540 3573 3540
Size: 33 7676
BamHI G'GATC_C
Cuts at: 788 1912 4734 788
Size: 1124 2822 3763
Fragments arranged by size:
3763 2822 1124
BanI G'GyrC_C
Cuts at: 3493 4725 5139 5387 5422 3493
Size: 1232 414 248 35 5780
Fragments arranged by size:
5780 1232 414 248 35
BanII G_rGCy'C
Cuts at: 2591 4280 4519 4723 5753 2591
Size: 1689 239 204 1030 4547
Fragments arranged by size:
4547 1689 1030 239 204
BbeI G_GCGC'C
Cuts at: 5143 5391 5143
Size: 248 7461
BbsI GAAGACnn'nnnn_
Cuts at: 1056 2294 2905 4142 1056
Size: 1238 611 1237 4623
Fragments arranged by size:
4623 1238 1237 611
BciVI GTATCCnnnnn_n'
Cuts at: 3159 3405 3598 3665 5607 6368 7709 3159
Size: 246 193 67 1942 761 1341 3159
Fragments arranged by size:
3159 1942 1341 761 246 193 67
BclI T'GATC_A
Cuts at: 939 5229 939
Size: 4290 3419
BfrBI ATG'CAT
Cuts at: 4399 4399
Size: 7709
BglII A'GATC_T
Cuts at: 1791 2989 5224 7261 1791
Size: 1198 2235 2037 2239
Fragments arranged by size:
2239 2235 2037 1198
BlpI GC'TnA_GC
Cuts at: 2813 4821 2813
Size: 2008 5701
Bme1580I G_kGCm'C
Cuts at: 1629 2591 3640 4519 5334 5427 6483 1629
Size: 962 1049 879 815 93 1056 2855
Fragments arranged by size:
2855 1056 1049 962 879 815 93
BmrI ACTGGGnnnn_n'
Cuts at: 1828 3535 3634 4626 4921 5002 5337 7481 1828
Size: 1707 99 992 295 81 335 2144 2056
Fragments arranged by size:
2144 2056 1707 992 335 295 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6076 6076
Size: 7709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 2591 2615 2781 3014 287
Size: 2304 24 166 233 4982
Fragments arranged by size:
4982 2304 233 166 24
Bpu10I CC'TnA_GC
Cuts at: 4237 4274 4237
Size: 37 7672
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4603 4705 4885 5764 6256 6554 3616
Size: 987 102 180 879 492 298 4771
Fragments arranged by size:
4771 987 879 492 298 180 102
BsaI GGTCTCn'nnnn_
Cuts at: 3514 4077 7413 3514
Size: 563 3336 3810
Fragments arranged by size:
3810 3336 563
BsaAI yAC'GTr
Cuts at: 448 1710 5062 448
Size: 1262 3352 3095
Fragments arranged by size:
3352 3095 1262
BsaBI GATnn'nnATC
Cuts at: 6 2841 5223 7260 6
Size: 2835 2382 2037 455
Fragments arranged by size:
2835 2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 5140 5388 6090 7586 5140
Size: 248 702 1496 5263
Fragments arranged by size:
5263 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4898 5419 6097 6371 6518 6929 4898
Size: 521 678 274 147 411 5678
Fragments arranged by size:
5678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1562 1592 1892 1922 1562
Size: 30 300 30 7349
Fragments arranged by size:
7349 300 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1913 1913
Size: 7709
BseYI C'CCAG_C
Cuts at: 1565 3401 3930 5134 5892 6469 6965 1565
Size: 1836 529 1204 758 577 496 2309
Fragments arranged by size:
2309 1836 1204 758 577 529 496
BsiEI CG_ry'CG
Cuts at: 104 5251 5297 6505 104
Size: 5147 46 1208 1308
Fragments arranged by size:
5147 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 867 4280 4723 5501 5691 6483 867
Size: 3413 443 778 190 792 2093
Fragments arranged by size:
3413 2093 792 778 443 190
BsmI GAATG_Cn'
Cuts at: 1219 3774 4632 1219
Size: 2555 858 4296
Fragments arranged by size:
4296 2555 858
Bsp1286I G_dGCh'C
Cuts at: 867 1629 2591 3640 4280 4519 4723 5334 5427
Size: 762 962 1049 640 239 204 611 93
Cuts at: 5427 5501 5691 5753 6483 867
Size: 74 190 62 730 2093
Fragments arranged by size:
2093 1049 962 762 730 640 611 239
204 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6929 6929
Size: 7709
BspHI T'CATG_A
Cuts at: 1866 4032 6885 1866
Size: 2166 2853 2690
Fragments arranged by size:
2853 2690 2166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 852 930 2899 3297 4759 4973 5275 5656 852
Size: 78 1969 398 1462 214 302 381 2905
Fragments arranged by size:
2905 1969 1462 398 381 302 214 78
BsrBI CCG'CTC
Cuts at: 50 6001 6055 7539 7704 50
Size: 5951 54 1484 165 55
Fragments arranged by size:
5951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 592 1160 1853 2158 3349 3734 4265 5621 592
Size: 568 693 305 1191 385 531 1356 2680
Fragments arranged by size:
2680 1356 1191 693 568 531 385 305
BsrFI r'CCGG_y
Cuts at: 3023 5707 5888 6097 3023
Size: 2684 181 209 4635
Fragments arranged by size:
4635 2684 209 181
BssSI C'ACGA_G
Cuts at: 5980 6338 5980
Size: 358 7351
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 2062 5086 1852
Size: 210 3024 4475
Fragments arranged by size:
4475 3024 210
BstBI TT'CG_AA
Cuts at: 129 1512 2697 6070 129
Size: 1383 1185 3373 1768
Fragments arranged by size:
3373 1768 1383 1185
BstXI CCAn_nnnn'nTGG
Cuts at: 3162 4260 4512 3162
Size: 1098 252 6359
Fragments arranged by size:
6359 1098 252
BstYI r'GATC_y
Cuts at: 1 788 1119 1791 1912 2760 2825 2989 3098
Size: 787 331 672 121 848 65 164 109
Cuts at: 3098 4359 4588 4734 5201 5224 5559 5805 6817
Size: 1261 229 146 467 23 335 246 1012
Cuts at: 6817 6903 6915 7261 1
Size: 86 12 346 449
Fragments arranged by size:
1261 1012 848 787 672 467 449 346
335 331 246 229 164 146 121 109
86 65 23 12
BstZ17I GTA'TAC
Cuts at: 248 683 3436 248
Size: 435 2753 4521
Fragments arranged by size:
4521 2753 435
Bsu36I CC'TnA_GG
Cuts at: 1546 3499 1546
Size: 1953 5756
BtgI C'CryG_G
Cuts at: 107 1341 1427 107
Size: 1234 86 6389
Fragments arranged by size:
6389 1234 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5659 5840 6003 5659
Size: 181 163 7365
Fragments arranged by size:
7365 181 163
BtsI GCAGTG_nn'
Cuts at: 3589 4952 3589
Size: 1363 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4247 4282 4247
Size: 35 7674
DraI TTT'AAA
Cuts at: 2730 4151 4708 2730
Size: 1421 557 5731
Fragments arranged by size:
5731 1421 557
DraIII CAC_nnn'GTG
Cuts at: 2144 2144
Size: 7709
DrdI GACnn_nn'nnGTC
Cuts at: 5415 6273 5415
Size: 858 6851
EaeI y'GGCC_r
Cuts at: 1338 5294 5468 5859 5886 7231 1338
Size: 3956 174 391 27 1345 1816
Fragments arranged by size:
3956 1816 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5294 5294
Size: 7709
EarI CTCTTCn'nnn_
Cuts at: 178 497 1406 1514 1709 1865 2033 3164 5732
Size: 319 909 108 195 156 168 1131 2568
Cuts at: 5732 5942 178
Size: 210 1945
Fragments arranged by size:
2568 1945 1131 909 319 210 195 168
156 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5917 6227 6373 7519 5917
Size: 310 146 1146 6107
Fragments arranged by size:
6107 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4278 4721 4278
Size: 443 7266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 287 478 2144 2174 2591 2615 2781 3014
Size: 128 191 1666 30 417 24 166 233
Cuts at: 3014 3074 3112 4127 5534 5966 6713 6981 159
Size: 60 38 1015 1407 432 747 268 887
Fragments arranged by size:
1666 1407 1015 887 747 432 417 268
233 191 166 128 60 38 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1932 4598 1932
Size: 2666 5043
EcoO109I rG'GnC_Cy
Cuts at: 520 1357 2587 4450 4848 520
Size: 837 1230 1863 398 3381
Fragments arranged by size:
3381 1863 1230 837 398
EcoRI G'AATT_C
Cuts at: 825 951 2487 2690 4713 825
Size: 126 1536 203 2023 3821
Fragments arranged by size:
3821 2023 1536 203 126
EcoRV GAT'ATC
Cuts at: 2511 2511
Size: 7709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 2452 2976 3431 5728 189
Size: 2263 524 455 2297 2170
Fragments arranged by size:
2297 2263 2170 524 455
FspI TGC'GCA
Cuts at: 5490 5490
Size: 7709
HaeII r_GCGC'y
Cuts at: 5143 5391 6413 7392 5143
Size: 248 1022 979 5460
Fragments arranged by size:
5460 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3492 3524 5551 5581 5583 5613 3492
Size: 32 2027 30 2 30 5588
Fragments arranged by size:
5588 2027 32 30 30 2
HincII GTy'rAC
Cuts at: 1026 2751 4748 1026
Size: 1725 1997 3987
Fragments arranged by size:
3987 1997 1725
HindIII A'AGCT_T
Cuts at: 991 1460 3189 4304 991
Size: 469 1729 1115 4396
Fragments arranged by size:
4396 1729 1115 469
KasI G'GCGC_C
Cuts at: 5139 5387 5139
Size: 248 7461
KpnI G_GTAC'C
Cuts at: 3497 4729 3497
Size: 1232 6477
MfeI C'AATT_G
Cuts at: 1205 2042 3552 3870 4670 1205
Size: 837 1510 318 800 4244
Fragments arranged by size:
4244 1510 837 800 318
MluI A'CGCG_T
Cuts at: 7142 7142
Size: 7709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 340 678 1706 1890 2667 6380 6564 340
Size: 338 1028 184 777 3713 184 1485
Fragments arranged by size:
3713 1485 1028 777 338 184 184
MscI TGG'CCA
Cuts at: 1340 5470 1340
Size: 4130 3579
MslI CAynn'nnrTG
Cuts at: 430 1988 3883 4181 4258 5698 5825 430
Size: 1558 1895 298 77 1440 127 2314
Fragments arranged by size:
2314 1895 1558 1440 298 127 77
MspA1I CmG'CkG
Cuts at: 3398 4649 4820 5134 5494 6507 6752 3398
Size: 1251 171 314 360 1013 245 4355
Fragments arranged by size:
4355 1251 1013 360 314 245 171
NaeI GCC'GGC
Cuts at: 5890 5890
Size: 7709
NarI GG'CG_CC
Cuts at: 5140 5388 5140
Size: 248 7461
NcoI C'CATG_G
Cuts at: 107 1341 1427 107
Size: 1234 86 6389
Fragments arranged by size:
6389 1234 86
NdeI CA'TA_TG
Cuts at: 947 4077 947
Size: 3130 4579
NgoMIV G'CCGG_C
Cuts at: 5888 5888
Size: 7709
NsiI A_TGCA'T
Cuts at: 4401 4401
Size: 7709
NspI r_CATG'y
Cuts at: 1725 1963 2060 2674 3744 4762 5262 6169 1725
Size: 238 97 614 1070 1018 500 907 3265
Fragments arranged by size:
3265 1070 1018 907 614 500 238 97
PciI A'CATG_T
Cuts at: 1721 6165 1721
Size: 4444 3265
PflMI CCAn_nnn'nTGG
Cuts at: 265 3946 265
Size: 3681 4028
PfoI T'CCnGG_A
Cuts at: 264 791 2631 3590 264
Size: 527 1840 959 4383
Fragments arranged by size:
4383 1840 959 527
PmeI GTTT'AAAC
Cuts at: 4708 4708
Size: 7709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 187 219 6848 6880 187
Size: 32 6629 32 1016
Fragments arranged by size:
6629 1016 32 32
PpuMI rG'GwC_Cy
Cuts at: 520 1357 4450 520
Size: 837 3093 3779
Fragments arranged by size:
3779 3093 837
PspOMI G'GGCC_C
Cuts at: 2587 4515 2587
Size: 1928 5781
PstI C_TGCA'G
Cuts at: 3366 3584 3825 4756 5441 3366
Size: 218 241 931 685 5634
Fragments arranged by size:
5634 931 685 241 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7709
PvuII CAG'CTG
Cuts at: 4649 5134 5494 4649
Size: 485 360 6864
Fragments arranged by size:
6864 485 360
RsrII CG'GwC_CG
Cuts at: 5904 5904
Size: 7709
SacI G_AGCT'C
Cuts at: 4280 4723 4280
Size: 443 7266
SalI G'TCGA_C
Cuts at: 4746 4746
Size: 7709
SapI GCTCTTCn'nnn_
Cuts at: 2033 5732 5942 2033
Size: 3699 210 3800
Fragments arranged by size:
3800 3699 210
SbfI CC_TGCA'GG
Cuts at: 4756 4756
Size: 7709
ScaI AGT'ACT
Cuts at: 2037 4636 7226 2037
Size: 2599 2590 2520
Fragments arranged by size:
2599 2590 2520
SexAI A'CCwGG_T
Cuts at: 1387 1387
Size: 7709
SfcI C'TryA_G
Cuts at: 33 199 1148 3362 3580 3659 3821 4286 4538
Size: 166 949 2214 218 79 162 465 252
Cuts at: 4538 4752 5437 6430 6621 33
Size: 214 685 993 191 1121
Fragments arranged by size:
2214 1121 993 949 685 465 252 218
214 191 166 162 79
SfoI GGC'GCC
Cuts at: 5141 5389 5141
Size: 248 7461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7709
SmaI CCC'GGG
Cuts at: 4731 4731
Size: 7709
SmlI C'TyrA_G
Cuts at: 3631 4582 4684 4864 5779 6271 6533 3631
Size: 951 102 180 915 492 262 4807
Fragments arranged by size:
4807 951 915 492 262 180 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7709
SphI G_CATG'C
Cuts at: 4762 5262 4762
Size: 500 7209
SspI AAT'ATT
Cuts at: 3474 4020 3474
Size: 546 7163
StuI AGG'CCT
Cuts at: 1938 4242 1938
Size: 2304 5405
StyI C'CwwG_G
Cuts at: 107 476 1341 1427 2249 4219 4341 4446 4594
Size: 369 865 86 822 1970 122 105 148
Cuts at: 4594 4843 5197 107
Size: 249 354 2619
Fragments arranged by size:
2619 1970 865 822 369 354 249 148
122 105 86
SwaI ATTT'AAAT
Cuts at: 4151 4151
Size: 7709
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3517 5292 5425 5884 6093 3517
Size: 1775 133 459 209 5133
Fragments arranged by size:
5133 1775 459 209 133
TatI w'GTAC_w
Cuts at: 1144 1718 2035 2074 4634 7224 1144
Size: 574 317 39 2560 2590 1629
Fragments arranged by size:
2590 2560 1629 574 317 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 692 724 2306 2338 3553 3585 7226 7258 692
Size: 32 1582 32 1215 32 3641 32 1143
Fragments arranged by size:
3641 1582 1215 1143 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 3013 5506 1801
Size: 1212 2493 4004
Fragments arranged by size:
4004 2493 1212
XbaI T'CTAG_A
Cuts at: 2347 3621 4740 2347
Size: 1274 1119 5316
Fragments arranged by size:
5316 1274 1119
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2805 5204 2805
Size: 2399 5310
XmaI C'CCGG_G
Cuts at: 4729 4729
Size: 7709
XmnI GAAnn'nnTTC
Cuts at: 2432 2534 2600 2432
Size: 102 66 7541
Fragments arranged by size:
7541 102 66
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BbvCI BcgI
BglI BmgBI BmtI BsgI BsiWI BsmBI BsrGI BssHII
BstEII ClaI FseI FspAI HpaI NheI NotI NruI
PacI PasI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI XhoI ZraI