Restriction map for
pF1KB3124
(Circular) (Six-base) MAPSORT of: pF1KB3124.seq Check: 7877 from: 1 to: 4781
pF1KB3124 4781 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 213 213
Size: 4781
AccI GT'mk_AC
Cuts at: 685 1819 685
Size: 1134 3647
Acc65I G'GTAC_C
Cuts at: 1400 1797 1400
Size: 397 4384
AclI AA'CG_TT
Cuts at: 549 1325 4621 549
Size: 776 3296 709
Fragments arranged by size:
3296 776 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1173 1185 1616 2606 3038 3785 4053 718
Size: 455 12 431 990 432 747 268 1446
Fragments arranged by size:
1446 990 747 455 432 431 268 12
AflIII A'CryG_T
Cuts at: 218 2133 3237 4214 218
Size: 1915 1104 977 785
Fragments arranged by size:
1915 1104 977 785
AgeI A'CCGG_T
Cuts at: 3169 3169
Size: 4781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 403 403
Size: 4781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 607
Size: 32 4749
AlwNI CAG_nnn'CTG
Cuts at: 1240 2086 3653 1240
Size: 846 1567 2368
Fragments arranged by size:
2368 1567 846
ApaLI G'TGCA_C
Cuts at: 3551 3551
Size: 4781
ApoI r'AATT_y
Cuts at: 427 510 1160 1300 1785 4134 4390 427
Size: 83 650 140 485 2349 256 818
Fragments arranged by size:
2349 818 650 485 256 140 83
AseI AT'TA_AT
Cuts at: 20 20
Size: 4781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
AvaI C'yCGr_G
Cuts at: 1216 1801 1216
Size: 585 4196
BamHI G'GATC_C
Cuts at: 1044 1806 1044
Size: 762 4019
BanI G'GyrC_C
Cuts at: 1400 1797 2211 2459 2494 1400
Size: 397 414 248 35 3687
Fragments arranged by size:
3687 414 397 248 35
BanII G_rGCy'C
Cuts at: 182 606 1795 2825 182
Size: 424 1189 1030 2138
Fragments arranged by size:
2138 1189 1030 424
BbeI G_GCGC'C
Cuts at: 2215 2463 2215
Size: 248 4533
BbsI GAAGACnn'nnnn_
Cuts at: 490 1728 490
Size: 1238 3543
BciVI GTATCCnnnnn_n'
Cuts at: 2679 3440 4781 2679
Size: 761 1341 2679
Fragments arranged by size:
2679 1341 761
BclI T'GATC_A
Cuts at: 270 2301 270
Size: 2031 2750
BfrBI ATG'CAT
Cuts at: 1540 1540
Size: 4781
BglII A'GATC_T
Cuts at: 281 528 2296 4333 281
Size: 247 1768 2037 729
Fragments arranged by size:
2037 1768 729 247
BlpI GC'TnA_GC
Cuts at: 154 780 1893 154
Size: 626 1113 3042
Fragments arranged by size:
3042 1113 626
Bme1580I G_kGCm'C
Cuts at: 1630 1716 2406 2499 3555 1630
Size: 86 690 93 1056 2856
Fragments arranged by size:
2856 1056 690 93 86
BmgBI CAC'GTC
Cuts at: 1557 1557
Size: 4781
BmrI ACTGGGnnnn_n'
Cuts at: 636 828 1993 2074 2409 4553 636
Size: 192 1165 81 335 2144 864
Fragments arranged by size:
2144 1165 864 335 192 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 4781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 718 634
Size: 84 4697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 296 758 1957 2836 3328 3626 166
Size: 130 462 1199 879 492 298 1321
Fragments arranged by size:
1321 1199 879 492 462 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 816 889 4485 816
Size: 73 3596 1112
Fragments arranged by size:
3596 1112 73
BsaAI yAC'GTr
Cuts at: 219 558 2134 219
Size: 339 1576 2866
Fragments arranged by size:
2866 1576 339
BsaBI GATnn'nnATC
Cuts at: 6 662 2295 4332 6
Size: 656 1633 2037 455
Fragments arranged by size:
2037 1633 656 455
BsaHI Gr'CG_yC
Cuts at: 210 2212 2460 3162 4658 210
Size: 2002 248 702 1496 333
Fragments arranged by size:
2002 1496 702 333 248
BsaWI w'CCGG_w
Cuts at: 1970 2491 3169 3443 3590 4001 1970
Size: 521 678 274 147 411 2750
Fragments arranged by size:
2750 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 607 637 785 815 313
Size: 30 264 30 148 30 4279
Fragments arranged by size:
4279 264 148 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 1717 337
Size: 1380 3401
BseYI C'CCAG_C
Cuts at: 1149 1663 2206 2964 3541 4037 1149
Size: 514 543 758 577 496 1893
Fragments arranged by size:
1893 758 577 543 514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 123
Size: 4781
BsiEI CG_ry'CG
Cuts at: 104 2323 2369 3577 104
Size: 2219 46 1208 1308
Fragments arranged by size:
2219 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 182 1197 1795 2573 2763 3555 155
Size: 27 1015 598 778 190 792 1381
Fragments arranged by size:
1381 1015 792 778 598 190 27
BsmI GAATG_Cn'
Cuts at: 1542 1542
Size: 4781
Bsp1286I G_dGCh'C
Cuts at: 155 182 606 1197 1630 1716 1795 2406 2499
Size: 27 424 591 433 86 79 611 93
Cuts at: 2499 2573 2763 2825 3555 155
Size: 74 190 62 730 1381
Fragments arranged by size:
1381 730 611 591 433 424 190 93
86 79 74 62 27
BspEI T'CCGG_A
Cuts at: 4001 4001
Size: 4781
BspHI T'CATG_A
Cuts at: 926 3957 926
Size: 3031 1750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 1388 1831 2045 2347 2728 310
Size: 1078 443 214 302 381 2363
Fragments arranged by size:
2363 1078 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 618 3073 3127 4611 4776 50
Size: 568 2455 54 1484 165 55
Fragments arranged by size:
2455 1484 568 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1368 2693 1368
Size: 1325 3456
BsrFI r'CCGG_y
Cuts at: 2779 2960 3169 2779
Size: 181 209 4391
Fragments arranged by size:
4391 209 181
BssSI C'ACGA_G
Cuts at: 256 743 886 3052 3410 256
Size: 487 143 2166 358 1627
Fragments arranged by size:
2166 1627 487 358 143
BstAPI GCAn_nnn'nTGC
Cuts at: 1328 2158 1328
Size: 830 3951
BstBI TT'CG_AA
Cuts at: 3142 3142
Size: 4781
BstXI CCAn_nnnn'nTGG
Cuts at: 1129 1129
Size: 4781
BstYI r'GATC_y
Cuts at: 1 281 528 954 1044 1806 2273 2296 2631
Size: 280 247 426 90 762 467 23 335
Cuts at: 2631 2877 3889 3975 3987 4333 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 762 467 449 426 346 335 280
247 246 90 86 23 12
BstZ17I GTA'TAC
Cuts at: 686 686
Size: 4781
BtgI C'CryG_G
Cuts at: 107 815 107
Size: 708 4073
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2731 2912 3075 2731
Size: 181 163 4437
Fragments arranged by size:
4437 181 163
BtsI GCAGTG_nn'
Cuts at: 155 873 1418 1630 2024 155
Size: 718 545 212 394 2912
Fragments arranged by size:
2912 718 545 394 212
ClaI AT'CG_AT
Cuts at: 1052 1052
Size: 4781
DraI TTT'AAA
Cuts at: 525 1780 525
Size: 1255 3526
DrdI GACnn_nn'nnGTC
Cuts at: 2487 3345 2487
Size: 858 3923
EaeI y'GGCC_r
Cuts at: 492 941 2366 2540 2931 2958 4303 492
Size: 449 1425 174 391 27 1345 970
Fragments arranged by size:
1425 1345 970 449 391 174 27
EagI C'GGCC_G
Cuts at: 2366 2366
Size: 4781
EarI CTCTTCn'nnn_
Cuts at: 538 1514 1663 2804 3014 538
Size: 976 149 1141 210 2305
Fragments arranged by size:
2305 1141 976 210 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 616 2989 3299 3445 4591 616
Size: 2373 310 146 1146 806
Fragments arranged by size:
2373 1146 806 310 146
EcoICRI GAG'CTC
Cuts at: 180 1793 180
Size: 1613 3168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 718 1173 1185 1616 2606 3038 3785 4053
Size: 84 455 12 431 990 432 747 268
Cuts at: 4053 634
Size: 1362
Fragments arranged by size:
1362 990 747 455 432 431 268 84
12
EcoO109I rG'GnC_Cy
Cuts at: 1018 1920 1018
Size: 902 3879
EcoRI G'AATT_C
Cuts at: 427 1785 427
Size: 1358 3423
EcoRV GAT'ATC
Cuts at: 1572 1572
Size: 4781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 460 2800 460
Size: 2340 2441
FspI TGC'GCA
Cuts at: 2562 2562
Size: 4781
HaeII r_GCGC'y
Cuts at: 161 2215 2463 3485 4464 161
Size: 2054 248 1022 979 478
Fragments arranged by size:
2054 1022 979 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 169 201 1479 1511 2623 2653 2655 2685 169
Size: 32 1278 32 1112 30 2 30 2265
Fragments arranged by size:
2265 1278 1112 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1464 1820 1464
Size: 356 4425
KasI G'GCGC_C
Cuts at: 2211 2459 2211
Size: 248 4533
KpnI G_GTAC'C
Cuts at: 1404 1801 1404
Size: 397 4384
MluI A'CGCG_T
Cuts at: 4214 4214
Size: 4781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1357 3452 3636 1357
Size: 2095 184 2502
Fragments arranged by size:
2502 2095 184
MscI TGG'CCA
Cuts at: 494 943 2542 494
Size: 449 1599 2733
Fragments arranged by size:
2733 1599 449
MslI CAynn'nnrTG
Cuts at: 1054 2770 2897 1054
Size: 1716 127 2938
Fragments arranged by size:
2938 1716 127
MspA1I CmG'CkG
Cuts at: 696 1153 1240 1892 2206 2566 3579 3824 696
Size: 457 87 652 314 360 1013 245 1653
Fragments arranged by size:
1653 1013 652 457 360 314 245 87
NaeI GCC'GGC
Cuts at: 2962 2962
Size: 4781
NarI GG'CG_CC
Cuts at: 2212 2460 2212
Size: 248 4533
NcoI C'CATG_G
Cuts at: 107 107
Size: 4781
NdeI CA'TA_TG
Cuts at: 489 489
Size: 4781
NgoMIV G'CCGG_C
Cuts at: 2960 2960
Size: 4781
NsiI A_TGCA'T
Cuts at: 1542 1542
Size: 4781
NspI r_CATG'y
Cuts at: 507 1086 1834 2334 3241 507
Size: 579 748 500 907 2047
Fragments arranged by size:
2047 907 748 579 500
PasI CC'CwG_GG
Cuts at: 291 291
Size: 4781
PciI A'CATG_T
Cuts at: 3237 3237
Size: 4781
PfoI T'CCnGG_A
Cuts at: 611 611
Size: 4781
PmeI GTTT'AAAC
Cuts at: 1780 1780
Size: 4781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 607 639 3920 3952 607
Size: 32 3281 32 1436
Fragments arranged by size:
3281 1436 32 32
PpuMI rG'GwC_Cy
Cuts at: 1018 1018
Size: 4781
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 628 660 628
Size: 32 4749
PstI C_TGCA'G
Cuts at: 1353 1828 2513 1353
Size: 475 685 3621
Fragments arranged by size:
3621 685 475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4781
PvuII CAG'CTG
Cuts at: 696 1153 1240 2206 2566 696
Size: 457 87 966 360 2911
Fragments arranged by size:
2911 966 457 360 87
RsrII CG'GwC_CG
Cuts at: 2976 2976
Size: 4781
SacI G_AGCT'C
Cuts at: 182 1795 182
Size: 1613 3168
SalI G'TCGA_C
Cuts at: 1818 1818
Size: 4781
SapI GCTCTTCn'nnn_
Cuts at: 1514 2804 3014 1514
Size: 1290 210 3281
Fragments arranged by size:
3281 1290 210
SbfI CC_TGCA'GG
Cuts at: 1828 1828
Size: 4781
ScaI AGT'ACT
Cuts at: 4298 4298
Size: 4781
SfcI C'TryA_G
Cuts at: 33 1349 1824 2509 3502 3693 33
Size: 1316 475 685 993 191 1121
Fragments arranged by size:
1316 1121 993 685 475 191
SfoI GGC'GCC
Cuts at: 2213 2461 2213
Size: 248 4533
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
SmaI CCC'GGG
Cuts at: 1803 1803
Size: 4781
SmlI C'TyrA_G
Cuts at: 181 311 773 1936 2851 3343 3605 181
Size: 130 462 1163 915 492 262 1357
Fragments arranged by size:
1357 1163 915 492 462 262 130
SpeI A'CTAG_T
Cuts at: 67 1246 67
Size: 1179 3602
SphI G_CATG'C
Cuts at: 1834 2334 1834
Size: 500 4281
StuI AGG'CCT
Cuts at: 1720 1720
Size: 4781
StyI C'CwwG_G
Cuts at: 107 948 1915 2269 107
Size: 841 967 354 2619
Fragments arranged by size:
2619 967 841 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1271 2364 2497 2956 3165 1026
Size: 245 1093 133 459 209 2642
Fragments arranged by size:
2642 1093 459 245 209 133
TatI w'GTAC_w
Cuts at: 329 4296 329
Size: 3967 814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 785 817 4298 4330 785
Size: 32 3481 32 1236
Fragments arranged by size:
3481 1236 32 32
Tth111I GACn'n_nGTC
Cuts at: 2578 2578
Size: 4781
XbaI T'CTAG_A
Cuts at: 1812 1812
Size: 4781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2276 2276
Size: 4781
XmaI C'CCGG_G
Cuts at: 1801 1801
Size: 4781
XmnI GAAnn'nnTTC
Cuts at: 703 976 1117 703
Size: 273 141 4367
Fragments arranged by size:
4367 273 141
ZraI GAC'GTC
Cuts at: 211 211
Size: 4781
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII Bsu36I CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NheI NotI NruI PacI
PflMI PmlI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI