Restriction map for
pF1KB3122
(Circular) (Six-base) MAPSORT of: pF1KB3122.seq Check: 3957 from: 1 to: 3767
pF1KB3122 3767 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 441 805 441
Size: 364 3403
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 1592 2024 2771 3039 476
Size: 1116 432 747 268 1204
Fragments arranged by size:
1204 1116 747 432 268
AflIII A'CryG_T
Cuts at: 1119 2223 3200 1119
Size: 1104 977 1686
Fragments arranged by size:
1686 1104 977
AgeI A'CCGG_T
Cuts at: 2155 2155
Size: 3767
AlwNI CAG_nnn'CTG
Cuts at: 454 1072 2639 454
Size: 618 1567 1582
Fragments arranged by size:
1582 1567 618
ApaLI G'TGCA_C
Cuts at: 2537 2537
Size: 3767
ApoI r'AATT_y
Cuts at: 226 771 3120 3376 226
Size: 545 2349 256 617
Fragments arranged by size:
2349 617 545 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 787 787
Size: 3767
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 738 783 1197 1445 1480 738
Size: 45 414 248 35 3025
Fragments arranged by size:
3025 414 248 45 35
BanII G_rGCy'C
Cuts at: 781 1811 781
Size: 1030 2737
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbvCI CC'TCA_GC
Cuts at: 476 476
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 1665 2426 3767 1665
Size: 761 1341 1665
Fragments arranged by size:
1665 1341 761
BclI T'GATC_A
Cuts at: 1287 1287
Size: 3767
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 1392 1485 2541 1392
Size: 93 1056 2618
Fragments arranged by size:
2618 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 527 432
Size: 95 3672
Bpu10I CC'TnA_GC
Cuts at: 476 476
Size: 3767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1822 2314 2612 943
Size: 879 492 298 2098
Fragments arranged by size:
2098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 1281 3318 6
Size: 1275 2037 455
Fragments arranged by size:
2037 1275 455
BsaHI Gr'CG_yC
Cuts at: 1198 1446 2148 3644 1198
Size: 248 702 1496 1321
Fragments arranged by size:
1496 1321 702 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2429 2576 2987 956
Size: 521 678 274 147 411 1736
Fragments arranged by size:
1736 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 669 669
Size: 3767
BseYI C'CCAG_C
Cuts at: 1192 1950 2527 3023 1192
Size: 758 577 496 1936
Fragments arranged by size:
1936 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1309 1355 2563 104
Size: 1205 46 1208 1308
Fragments arranged by size:
1308 1208 1205 46
BsiHKAI G_wGCw'C
Cuts at: 295 781 1559 1749 2541 295
Size: 486 778 190 792 1521
Fragments arranged by size:
1521 792 778 486 190
BsmBI CGTCTCn'nnnn_
Cuts at: 588 588
Size: 3767
Bsp1286I G_dGCh'C
Cuts at: 295 781 1392 1485 1559 1749 1811 2541 295
Size: 486 611 93 74 190 62 730 1521
Fragments arranged by size:
1521 730 611 486 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2987 2987
Size: 3767
BspHI T'CATG_A
Cuts at: 2943 2943
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 656 817 1031 1333 1714 656
Size: 161 214 302 381 2709
Fragments arranged by size:
2709 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 2155 1765
Size: 181 209 3377
Fragments arranged by size:
3377 209 181
BsrGI T'GTAC_A
Cuts at: 232 232
Size: 3767
BssSI C'ACGA_G
Cuts at: 2038 2396 2038
Size: 358 3409
BstAPI GCAn_nnn'nTGC
Cuts at: 1144 1144
Size: 3767
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstEII G'GTnAC_C
Cuts at: 747 747
Size: 3767
BstXI CCAn_nnnn'nTGG
Cuts at: 735 735
Size: 3767
BstYI r'GATC_y
Cuts at: 1 792 1259 1282 1617 1863 2875 2961 2973
Size: 791 467 23 335 246 1012 86 12
Cuts at: 2973 3319 1
Size: 346 449
Fragments arranged by size:
1012 791 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3767
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 537 1010 537
Size: 473 3294
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 1473 2331 1473
Size: 858 2909
EaeI y'GGCC_r
Cuts at: 636 1352 1526 1917 1944 3289 636
Size: 716 174 391 27 1345 1114
Fragments arranged by size:
1345 1114 716 391 174 27
EagI C'GGCC_G
Cuts at: 1352 1352
Size: 3767
EarI CTCTTCn'nnn_
Cuts at: 385 1790 2000 385
Size: 1405 210 2152
Fragments arranged by size:
2152 1405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2285 2431 3577 1975
Size: 310 146 1146 2165
Fragments arranged by size:
2165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 476 527 1592 2024 2771 3039 432
Size: 44 51 1065 432 747 268 1160
Fragments arranged by size:
1160 1065 747 432 268 51 44
EcoO109I rG'GnC_Cy
Cuts at: 906 906
Size: 3767
EcoRI G'AATT_C
Cuts at: 226 771 226
Size: 545 3222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 272 1786 272
Size: 1514 2253
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 573 1201 1449 2471 3450 573
Size: 628 248 1022 979 890
Fragments arranged by size:
1022 979 890 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 568 600 1609 1639 1641 1671 568
Size: 32 1009 30 2 30 2664
Fragments arranged by size:
2664 1009 32 30 30 2
HincII GTy'rAC
Cuts at: 708 806 708
Size: 98 3669
HindIII A'AGCT_T
Cuts at: 254 254
Size: 3767
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MfeI C'AATT_G
Cuts at: 600 600
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 737 2438 2622 737
Size: 1701 184 1882
Fragments arranged by size:
1882 1701 184
MscI TGG'CCA
Cuts at: 638 1528 638
Size: 890 2877
MslI CAynn'nnrTG
Cuts at: 733 1756 1883 733
Size: 1023 127 2617
Fragments arranged by size:
2617 1023 127
MspA1I CmG'CkG
Cuts at: 878 1192 1552 2565 2810 878
Size: 314 360 1013 245 1835
Fragments arranged by size:
1835 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NcoI C'CATG_G
Cuts at: 107 107
Size: 3767
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NspI r_CATG'y
Cuts at: 820 1320 2227 820
Size: 500 907 2360
Fragments arranged by size:
2360 907 500
PciI A'CATG_T
Cuts at: 2223 2223
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 3735
PstI C_TGCA'G
Cuts at: 653 814 1499 653
Size: 161 685 2921
Fragments arranged by size:
2921 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 1192 1552 1192
Size: 360 3407
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 649 810 1495 2488 2679 33
Size: 616 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 616 191 161
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 922 1837 2329 2591 922
Size: 915 492 262 2098
Fragments arranged by size:
2098 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
SspI AAT'ATT
Cuts at: 489 489
Size: 3767
StyI C'CwwG_G
Cuts at: 107 901 1255 107
Size: 794 354 2619
Fragments arranged by size:
2619 794 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 232 3282 232
Size: 3050 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 703 1564 703
Size: 861 2906
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1262 1262
Size: 3767
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsgI BsiWI BsmI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI