Restriction map for
pF1KB3105
(Circular) (Six-base) MAPSORT of: pF1KB3105.seq Check: 7381 from: 1 to: 3605
pF1KB3105 3605 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 643 643
Size: 3605
Acc65I G'GTAC_C
Cuts at: 621 621
Size: 3605
AclI AA'CG_TT
Cuts at: 3445 3445
Size: 3605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1862 2609 2877 1430
Size: 432 747 268 2158
Fragments arranged by size:
2158 747 432 268
AflIII A'CryG_T
Cuts at: 957 2061 3038 957
Size: 1104 977 1524
Fragments arranged by size:
1524 1104 977
AgeI A'CCGG_T
Cuts at: 1993 1993
Size: 3605
AlwNI CAG_nnn'CTG
Cuts at: 910 2477 910
Size: 1567 2038
ApaLI G'TGCA_C
Cuts at: 2375 2375
Size: 3605
ApoI r'AATT_y
Cuts at: 262 609 2958 3214 262
Size: 347 2349 256 653
Fragments arranged by size:
2349 653 347 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
AvaI C'yCGr_G
Cuts at: 275 407 527 625 275
Size: 132 120 98 3255
Fragments arranged by size:
3255 132 120 98
BamHI G'GATC_C
Cuts at: 630 630
Size: 3605
BanI G'GyrC_C
Cuts at: 621 1035 1283 1318 621
Size: 414 248 35 2908
Fragments arranged by size:
2908 414 248 35
BanII G_rGCy'C
Cuts at: 619 1649 619
Size: 1030 2575
BbeI G_GCGC'C
Cuts at: 1039 1287 1039
Size: 248 3357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3571
BciVI GTATCCnnnnn_n'
Cuts at: 1503 2264 3605 1503
Size: 761 1341 1503
Fragments arranged by size:
1503 1341 761
BclI T'GATC_A
Cuts at: 1125 1125
Size: 3605
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3605
BglII A'GATC_T
Cuts at: 448 1120 3157 448
Size: 672 2037 896
Fragments arranged by size:
2037 896 672
BlpI GC'TnA_GC
Cuts at: 717 717
Size: 3605
Bme1580I G_kGCm'C
Cuts at: 1230 1323 2379 1230
Size: 93 1056 2456
Fragments arranged by size:
2456 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 817 898 1233 3377 817
Size: 81 335 2144 1045
Fragments arranged by size:
2144 1045 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 449 1972 449
Size: 1523 2082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1660 2152 2450 781
Size: 879 492 298 1936
Fragments arranged by size:
1936 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 572 3309 572
Size: 2737 868
BsaAI yAC'GTr
Cuts at: 958 958
Size: 3605
BsaBI GATnn'nnATC
Cuts at: 6 416 1119 3156 6
Size: 410 703 2037 455
Fragments arranged by size:
2037 703 455 410
BsaHI Gr'CG_yC
Cuts at: 1036 1284 1986 3482 1036
Size: 248 702 1496 1159
Fragments arranged by size:
1496 1159 702 248
BsaWI w'CCGG_w
Cuts at: 794 1315 1993 2267 2414 2825 794
Size: 521 678 274 147 411 1574
Fragments arranged by size:
1574 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 441 441
Size: 3605
BseYI C'CCAG_C
Cuts at: 1030 1788 2365 2861 1030
Size: 758 577 496 1774
Fragments arranged by size:
1774 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1147 1193 2401 104
Size: 1043 46 1208 1308
Fragments arranged by size:
1308 1208 1043 46
BsiHKAI G_wGCw'C
Cuts at: 619 1397 1587 2379 619
Size: 778 190 792 1845
Fragments arranged by size:
1845 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 619 1230 1323 1397 1587 1649 2379 619
Size: 611 93 74 190 62 730 1845
Fragments arranged by size:
1845 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2825 2825
Size: 3605
BspHI T'CATG_A
Cuts at: 2781 2781
Size: 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 476 655 869 1171 1552 476
Size: 179 214 302 381 2529
Fragments arranged by size:
2529 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 1897 1951 3435 3600 50
Size: 1847 54 1484 165 55
Fragments arranged by size:
1847 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1517 1517
Size: 3605
BsrFI r'CCGG_y
Cuts at: 1603 1784 1993 1603
Size: 181 209 3215
Fragments arranged by size:
3215 209 181
BssSI C'ACGA_G
Cuts at: 1876 2234 1876
Size: 358 3247
BstAPI GCAn_nnn'nTGC
Cuts at: 289 982 289
Size: 693 2912
BstBI TT'CG_AA
Cuts at: 1966 1966
Size: 3605
BstYI r'GATC_y
Cuts at: 1 270 448 630 1097 1120 1455 1701 2713
Size: 269 178 182 467 23 335 246 1012
Cuts at: 2713 2799 2811 3157 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 269 246 182
178 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 1736 1899 1555
Size: 181 163 3261
Fragments arranged by size:
3261 181 163
BtsI GCAGTG_nn'
Cuts at: 848 848
Size: 3605
DraI TTT'AAA
Cuts at: 604 604
Size: 3605
DraIII CAC_nnn'GTG
Cuts at: 322 322
Size: 3605
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2169 1311
Size: 858 2747
EaeI y'GGCC_r
Cuts at: 1190 1364 1755 1782 3127 1190
Size: 174 391 27 1345 1668
Fragments arranged by size:
1668 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1190 1190
Size: 3605
EarI CTCTTCn'nnn_
Cuts at: 504 1628 1838 504
Size: 1124 210 2271
Fragments arranged by size:
2271 1124 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1813 2123 2269 3415 1813
Size: 310 146 1146 2003
Fragments arranged by size:
2003 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 617
Size: 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1862 2609 2877 1430
Size: 432 747 268 2158
Fragments arranged by size:
2158 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 561 561
Size: 3605
EcoO109I rG'GnC_Cy
Cuts at: 488 744 488
Size: 256 3349
EcoRI G'AATT_C
Cuts at: 609 609
Size: 3605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 1624
Size: 3605
FspI TGC'GCA
Cuts at: 1386 1386
Size: 3605
HaeII r_GCGC'y
Cuts at: 1039 1287 2309 3288 1039
Size: 248 1022 979 1356
Fragments arranged by size:
1356 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1477 1479 1509 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 30 30 2
HincII GTy'rAC
Cuts at: 644 644
Size: 3605
HindIII A'AGCT_T
Cuts at: 465 465
Size: 3605
KasI G'GCGC_C
Cuts at: 1035 1283 1035
Size: 248 3357
KpnI G_GTAC'C
Cuts at: 625 625
Size: 3605
MluI A'CGCG_T
Cuts at: 3038 3038
Size: 3605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2276 2460 2276
Size: 184 3421
MscI TGG'CCA
Cuts at: 1366 1366
Size: 3605
MslI CAynn'nnrTG
Cuts at: 1594 1721 1594
Size: 127 3478
MspA1I CmG'CkG
Cuts at: 286 716 1030 1390 2403 2648 286
Size: 430 314 360 1013 245 1243
Fragments arranged by size:
1243 1013 430 360 314 245
NaeI GCC'GGC
Cuts at: 1786 1786
Size: 3605
NarI GG'CG_CC
Cuts at: 1036 1284 1036
Size: 248 3357
NgoMIV G'CCGG_C
Cuts at: 1784 1784
Size: 3605
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3605
NspI r_CATG'y
Cuts at: 658 1158 2065 658
Size: 500 907 2198
Fragments arranged by size:
2198 907 500
PciI A'CATG_T
Cuts at: 2061 2061
Size: 3605
PmeI GTTT'AAAC
Cuts at: 604 604
Size: 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 3573
PpuMI rG'GwC_Cy
Cuts at: 488 488
Size: 3605
PstI C_TGCA'G
Cuts at: 652 1337 652
Size: 685 2920
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3605
PvuII CAG'CTG
Cuts at: 286 1030 1390 286
Size: 744 360 2501
Fragments arranged by size:
2501 744 360
RsrII CG'GwC_CG
Cuts at: 1800 1800
Size: 3605
SacI G_AGCT'C
Cuts at: 619 619
Size: 3605
SalI G'TCGA_C
Cuts at: 642 642
Size: 3605
SapI GCTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
SbfI CC_TGCA'GG
Cuts at: 652 652
Size: 3605
ScaI AGT'ACT
Cuts at: 3122 3122
Size: 3605
SfcI C'TryA_G
Cuts at: 33 648 1333 2326 2517 33
Size: 615 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 191
SfoI GGC'GCC
Cuts at: 1037 1285 1037
Size: 248 3357
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
SmaI CCC'GGG
Cuts at: 627 627
Size: 3605
SmlI C'TyrA_G
Cuts at: 407 760 1675 2167 2429 407
Size: 353 915 492 262 1583
Fragments arranged by size:
1583 915 492 353 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3605
SphI G_CATG'C
Cuts at: 658 1158 658
Size: 500 3105
StyI C'CwwG_G
Cuts at: 739 1093 739
Size: 354 3251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1188 1321 1780 1989 1188
Size: 133 459 209 2804
Fragments arranged by size:
2804 459 209 133
TatI w'GTAC_w
Cuts at: 3120 3120
Size: 3605
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 532 564 3122 3154 532
Size: 32 2558 32 983
Fragments arranged by size:
2558 983 32 32
Tth111I GACn'n_nGTC
Cuts at: 1402 1402
Size: 3605
XbaI T'CTAG_A
Cuts at: 496 636 496
Size: 140 3465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 389 1100 389
Size: 711 2894
XhoI C'TCGA_G
Cuts at: 407 407
Size: 3605
XmaI C'CCGG_G
Cuts at: 625 625
Size: 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI