Restriction map for
pF1KB3060
(Circular) (Six-base) MAPSORT of: pF1KB3060.seq Check: 8436 from: 1 to: 4991
pF1KB3060 4991 bp
With 182 enzymes: SgfI *
April 17, 2013 10:25 ..
AccI GT'mk_AC
Cuts at: 2029 2029
Size: 4991
Acc65I G'GTAC_C
Cuts at: 2007 2007
Size: 4991
AclI AA'CG_TT
Cuts at: 1874 4831 1874
Size: 2957 2034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1803 2816 3248 3995 4263 1035
Size: 768 1013 432 747 268 1763
Fragments arranged by size:
1763 1013 768 747 432 268
AflIII A'CryG_T
Cuts at: 2343 3447 4424 2343
Size: 1104 977 2910
Fragments arranged by size:
2910 1104 977
AgeI A'CCGG_T
Cuts at: 3379 3379
Size: 4991
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 451 493 451
Size: 42 4949
AlwNI CAG_nnn'CTG
Cuts at: 126 579 861 1357 2296 3863 126
Size: 453 282 496 939 1567 1254
Fragments arranged by size:
1567 1254 939 496 453 282
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 1995 4344 4600 1995
Size: 2349 256 2386
Fragments arranged by size:
2386 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 1283 2011 1283
Size: 728 4263
BamHI G'GATC_C
Cuts at: 1190 2016 1190
Size: 826 4165
BanI G'GyrC_C
Cuts at: 288 964 1036 2007 2421 2669 2704 288
Size: 676 72 971 414 248 35 2575
Fragments arranged by size:
2575 971 676 414 248 72 35
BanII G_rGCy'C
Cuts at: 1460 1555 2005 3035 1460
Size: 95 450 1030 3416
Fragments arranged by size:
3416 1030 450 95
BbeI G_GCGC'C
Cuts at: 2425 2673 2425
Size: 248 4743
BbsI GAAGACnn'nnnn_
Cuts at: 1165 1353 1492 1809 1165
Size: 188 139 317 4347
Fragments arranged by size:
4347 317 188 139
BbvCI CC'TCA_GC
Cuts at: 590 590
Size: 4991
BciVI GTATCCnnnnn_n'
Cuts at: 1019 2889 3650 4991 1019
Size: 1870 761 1341 1019
Fragments arranged by size:
1870 1341 1019 761
BclI T'GATC_A
Cuts at: 1977 2511 1977
Size: 534 4457
BfrBI ATG'CAT
Cuts at: 1634 1634
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 2103 2103
Size: 4991
Bme1580I G_kGCm'C
Cuts at: 755 1832 2616 2709 3765 755
Size: 1077 784 93 1056 1981
Fragments arranged by size:
1981 1077 1056 784 93
BmrI ACTGGGnnnn_n'
Cuts at: 258 1222 1456 1747 2203 2284 2619 4763 258
Size: 964 234 291 456 81 335 2144 486
Fragments arranged by size:
2144 964 486 456 335 291 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1155 1942 3358 1155
Size: 787 1416 2788
Fragments arranged by size:
2788 1416 787
Bpu10I CC'TnA_GC
Cuts at: 590 916 1594 590
Size: 326 678 3987
Fragments arranged by size:
3987 678 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3046 3538 3836 2167
Size: 879 492 298 3322
Fragments arranged by size:
3322 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1126 4695 1126
Size: 3569 1422
BsaAI yAC'GTr
Cuts at: 225 1469 2344 225
Size: 1244 875 2872
Fragments arranged by size:
2872 1244 875
BsaBI GATnn'nnATC
Cuts at: 6 553 2505 4542 6
Size: 547 1952 2037 455
Fragments arranged by size:
2037 1952 547 455
BsaHI Gr'CG_yC
Cuts at: 2422 2670 3372 4868 2422
Size: 248 702 1496 2545
Fragments arranged by size:
2545 1496 702 248
BsaWI w'CCGG_w
Cuts at: 137 323 2180 2701 3379 3653 3800 4211 137
Size: 186 1857 521 678 274 147 411 917
Fragments arranged by size:
1857 917 678 521 411 274 186 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1859 1889 1859
Size: 30 4961
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1366 1366
Size: 4991
BseYI C'CCAG_C
Cuts at: 199 1307 1671 1947 2416 3174 3751 4247 199
Size: 1108 364 276 469 758 577 496 943
Fragments arranged by size:
1108 943 758 577 496 469 364 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 1725 1708
Size: 17 4974
BsiEI CG_ry'CG
Cuts at: 104 2533 2579 3787 104
Size: 2429 46 1208 1308
Fragments arranged by size:
2429 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1442 1555 2005 2783 2973 3765 1442
Size: 113 450 778 190 792 2668
Fragments arranged by size:
2668 792 778 450 190 113
Bsp1286I G_dGCh'C
Cuts at: 755 1442 1460 1555 1832 2005 2616 2709 2783
Size: 687 18 95 277 173 611 93 74
Cuts at: 2783 2973 3035 3765 755
Size: 190 62 730 1981
Fragments arranged by size:
1981 730 687 611 277 190 173 95
93 74 62 18
BspEI T'CCGG_A
Cuts at: 137 4211 137
Size: 4074 917
BspHI T'CATG_A
Cuts at: 4167 4167
Size: 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 1144 2041 2255 2557 2938 425
Size: 719 897 214 302 381 2478
Fragments arranged by size:
2478 897 719 381 302 214
BsrBI CCG'CTC
Cuts at: 50 168 3283 3337 4821 4986 50
Size: 118 3115 54 1484 165 55
Fragments arranged by size:
3115 1484 165 118 55 54
BsrDI GCAATG_nn'
Cuts at: 428 2903 428
Size: 2475 2516
BsrFI r'CCGG_y
Cuts at: 291 2989 3170 3379 291
Size: 2698 181 209 1903
Fragments arranged by size:
2698 1903 209 181
BssSI C'ACGA_G
Cuts at: 213 604 1639 3262 3620 213
Size: 391 1035 1623 358 1584
Fragments arranged by size:
1623 1584 1035 391 358
BstAPI GCAn_nnn'nTGC
Cuts at: 126 2368 126
Size: 2242 2749
BstBI TT'CG_AA
Cuts at: 3352 3352
Size: 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 336 543 648 336
Size: 207 105 4679
Fragments arranged by size:
4679 207 105
BstYI r'GATC_y
Cuts at: 1 1190 2016 2483 2506 2841 3087 4099 4185
Size: 1189 826 467 23 335 246 1012 86
Cuts at: 4185 4197 4543 1
Size: 12 346 449
Fragments arranged by size:
1189 1012 826 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 401 542 1609 107
Size: 294 141 1067 3489
Fragments arranged by size:
3489 1067 294 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2941 3122 3285 2941
Size: 181 163 4647
Fragments arranged by size:
4647 181 163
BtsI GCAGTG_nn'
Cuts at: 269 1784 1830 2234 269
Size: 1515 46 404 3026
Fragments arranged by size:
3026 1515 404 46
DraI TTT'AAA
Cuts at: 1128 1990 1128
Size: 862 4129
DraIII CAC_nnn'GTG
Cuts at: 1763 1763
Size: 4991
DrdI GACnn_nn'nnGTC
Cuts at: 2697 3555 2697
Size: 858 4133
EaeI y'GGCC_r
Cuts at: 452 1901 2576 2750 3141 3168 4513 452
Size: 1449 675 174 391 27 1345 930
Fragments arranged by size:
1449 1345 930 675 391 174 27
EagI C'GGCC_G
Cuts at: 2576 2576
Size: 4991
EarI CTCTTCn'nnn_
Cuts at: 623 692 1054 1180 1384 1933 3014 3224 623
Size: 69 362 126 204 549 1081 210 2390
Fragments arranged by size:
2390 1081 549 362 210 204 126 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 3199 3509 3655 4801 358
Size: 2841 310 146 1146 548
Fragments arranged by size:
2841 1146 548 310 146
EcoICRI GAG'CTC
Cuts at: 1553 2003 1553
Size: 450 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1803 2816 3248 3995 4263 1035
Size: 768 1013 432 747 268 1763
Fragments arranged by size:
1763 1013 768 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 118 118
Size: 4991
EcoO109I rG'GnC_Cy
Cuts at: 123 878 2130 123
Size: 755 1252 2984
Fragments arranged by size:
2984 1252 755
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1422 3010 1422
Size: 1588 3403
FspI TGC'GCA
Cuts at: 439 2772 439
Size: 2333 2658
HaeII r_GCGC'y
Cuts at: 2425 2673 3695 4674 2425
Size: 248 1022 979 2742
Fragments arranged by size:
2742 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1125 1157 2833 2863 2865 2895 1125
Size: 32 1676 30 2 30 3221
Fragments arranged by size:
3221 1676 32 30 30 2
HincII GTy'rAC
Cuts at: 1872 2030 1872
Size: 158 4833
KasI G'GCGC_C
Cuts at: 2421 2669 2421
Size: 248 4743
KpnI G_GTAC'C
Cuts at: 2011 2011
Size: 4991
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 236 548 644 728 3662 3846 236
Size: 312 96 84 2934 184 1381
Fragments arranged by size:
2934 1381 312 184 96 84
MscI TGG'CCA
Cuts at: 1903 2752 1903
Size: 849 4142
MslI CAynn'nnrTG
Cuts at: 388 541 547 724 1078 1261 1639 2980 3107
Size: 153 6 177 354 183 378 1341 127
Cuts at: 3107 388
Size: 2272
Fragments arranged by size:
2272 1341 378 354 183 177 153 127
6
MspA1I CmG'CkG
Cuts at: 1088 2102 2416 2776 3789 4034 1088
Size: 1014 314 360 1013 245 2045
Fragments arranged by size:
2045 1014 1013 360 314 245
NaeI GCC'GGC
Cuts at: 293 3172 293
Size: 2879 2112
NarI GG'CG_CC
Cuts at: 2422 2670 2422
Size: 248 4743
NcoI C'CATG_G
Cuts at: 107 542 107
Size: 435 4556
NgoMIV G'CCGG_C
Cuts at: 291 3170 291
Size: 2879 2112
NsiI A_TGCA'T
Cuts at: 1636 1636
Size: 4991
NspI r_CATG'y
Cuts at: 474 1634 2044 2544 3451 474
Size: 1160 410 500 907 2014
Fragments arranged by size:
2014 1160 907 500 410
PasI CC'CwG_GG
Cuts at: 1496 1496
Size: 4991
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 1496 1496
Size: 4991
PfoI T'CCnGG_A
Cuts at: 986 986
Size: 4991
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PmlI CAC'GTG
Cuts at: 1469 1469
Size: 4991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4959
PpuMI rG'GwC_Cy
Cuts at: 878 878
Size: 4991
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 856 888 856
Size: 32 4959
PstI C_TGCA'G
Cuts at: 122 282 575 2038 2723 122
Size: 160 293 1463 685 2390
Fragments arranged by size:
2390 1463 685 293 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 1088 2416 2776 1088
Size: 1328 360 3303
Fragments arranged by size:
3303 1328 360
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 1555 2005 1555
Size: 450 4541
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 349 4508 349
Size: 4159 832
SexAI A'CCwGG_T
Cuts at: 1039 1039
Size: 4991
SfcI C'TryA_G
Cuts at: 33 118 278 571 2034 2719 3712 3903 33
Size: 85 160 293 1463 685 993 191 1121
Fragments arranged by size:
1463 1121 993 685 293 191 160 85
SfoI GGC'GCC
Cuts at: 2423 2671 2423
Size: 248 4743
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 1285 2013 1285
Size: 728 4263
SmlI C'TyrA_G
Cuts at: 2146 3061 3553 3815 2146
Size: 915 492 262 3322
Fragments arranged by size:
3322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 474 1634 2044 2544 474
Size: 1160 410 500 2921
Fragments arranged by size:
2921 1160 500 410
StuI AGG'CCT
Cuts at: 211 211
Size: 4991
StyI C'CwwG_G
Cuts at: 107 542 1365 2125 2479 107
Size: 435 823 760 354 2619
Fragments arranged by size:
2619 823 760 435 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 824 1468 2574 2707 3166 3375 240
Size: 584 644 1106 133 459 209 1856
Fragments arranged by size:
1856 1106 644 584 459 209 133
TatI w'GTAC_w
Cuts at: 245 347 872 4506 245
Size: 102 525 3634 730
Fragments arranged by size:
3634 730 525 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 4959
Tth111I GACn'n_nGTC
Cuts at: 2788 2788
Size: 4991
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 540 2486 369
Size: 171 1946 2874
Fragments arranged by size:
2874 1946 171
XmaI C'CCGG_G
Cuts at: 1283 2011 1283
Size: 728 4263
XmnI GAAnn'nnTTC
Cuts at: 1477 1477
Size: 4991
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BpmI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI