Restriction map for
pF1KB1488
(Circular) (Six-base) MAPSORT of: pF1KB1488.seq Check: 7076 from: 1 to: 8345
pF1KB1488 8345 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2618 2754 2618
Size: 136 8209
AatII G_ACGT'C
Cuts at: 4571 4571
Size: 8345
AccI GT'mk_AC
Cuts at: 5383 5383
Size: 8345
Acc65I G'GTAC_C
Cuts at: 578 5361 578
Size: 4783 3562
AclI AA'CG_TT
Cuts at: 8185 8185
Size: 8345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1490 2023 2860 4261 4883 6170 6602 7349
Size: 114 533 837 1401 622 1287 432 747
Cuts at: 7349 7617 1376
Size: 268 2104
Fragments arranged by size:
2104 1401 1287 837 747 622 533 432
268 114
AfeI AGC'GCT
Cuts at: 2209 2209
Size: 8345
AflIII A'CryG_T
Cuts at: 2230 2711 5697 6801 7778 2230
Size: 481 2986 1104 977 2797
Fragments arranged by size:
2986 2797 1104 977 481
AgeI A'CCGG_T
Cuts at: 6733 6733
Size: 8345
AhdI GACnn_n'nnGTC
Cuts at: 468 468
Size: 8345
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1846 1878 1846
Size: 32 8313
AlwNI CAG_nnn'CTG
Cuts at: 1113 1303 1453 2769 3335 3808 5211 5650 7217
Size: 190 150 1316 566 473 1403 439 1567
Cuts at: 7217 1113
Size: 2241
Fragments arranged by size:
2241 1567 1403 1316 566 473 439 190
150
ApaI G_GGCC'C
Cuts at: 1254 4667 1254
Size: 3413 4932
ApaLI G'TGCA_C
Cuts at: 4504 7115 4504
Size: 2611 5734
ApoI r'AATT_y
Cuts at: 2866 5349 7698 7954 2866
Size: 2483 2349 256 3257
Fragments arranged by size:
3257 2483 2349 256
AseI AT'TA_AT
Cuts at: 20 2168 20
Size: 2148 6197
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8345
AvaI C'yCGr_G
Cuts at: 248 612 1516 1640 2852 3128 3223 4703 4792
Size: 364 904 124 1212 276 95 1480 89
Cuts at: 4792 5191 5365 248
Size: 399 174 3228
Fragments arranged by size:
3228 1480 1212 904 399 364 276 174
124 95 89
BamHI G'GATC_C
Cuts at: 3272 5370 3272
Size: 2098 6247
BanI G'GyrC_C
Cuts at: 372 524 578 870 1719 1837 2772 2811 3673
Size: 152 54 292 849 118 935 39 862
Cuts at: 3673 3720 4470 4821 5330 5361 5775 6023 6058
Size: 47 750 351 509 31 414 248 35
Cuts at: 6058 372
Size: 2659
Fragments arranged by size:
2659 935 862 849 750 509 414 351
292 248 152 118 54 47 39 35
31
BanII G_rGCy'C
Cuts at: 965 1178 1254 1673 1826 2300 2379 2785 3668
Size: 213 76 419 153 474 79 406 883
Cuts at: 3668 3802 4667 5359 6389 965
Size: 134 865 692 1030 2921
Fragments arranged by size:
2921 1030 883 865 692 474 419 406
213 153 134 79 76
BbeI G_GCGC'C
Cuts at: 3724 4474 5779 6027 3724
Size: 750 1305 248 6042
Fragments arranged by size:
6042 1305 750 248
BbsI GAAGACnn'nnnn_
Cuts at: 991 1328 2131 3564 5015 991
Size: 337 803 1433 1451 4321
Fragments arranged by size:
4321 1451 1433 803 337
BbvCI CC'TCA_GC
Cuts at: 4880 4880
Size: 8345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4542 4576 5105 5139 4542
Size: 34 529 34 7748
Fragments arranged by size:
7748 529 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6243 7004 8345 6243
Size: 761 1341 6243
Fragments arranged by size:
6243 1341 761
BclI T'GATC_A
Cuts at: 5159 5865 5159
Size: 706 7639
BglI GCCn_nnn'nGGC
Cuts at: 1903 2363 3694 4746 1903
Size: 460 1331 1052 5502
Fragments arranged by size:
5502 1331 1052 460
BglII A'GATC_T
Cuts at: 749 5860 7897 749
Size: 5111 2037 1197
Fragments arranged by size:
5111 2037 1197
BlpI GC'TnA_GC
Cuts at: 1825 5457 1825
Size: 3632 4713
Bme1580I G_kGCm'C
Cuts at: 248 377 527 1254 2037 2816 3678 4508 4667
Size: 129 150 727 783 779 862 830 159
Cuts at: 4667 4824 4894 5292 5970 6063 7119 248
Size: 157 70 398 678 93 1056 1474
Fragments arranged by size:
1474 1056 862 830 783 779 727 678
398 159 157 150 129 93 70
BmgBI CAC'GTC
Cuts at: 2233 4326 4671 2233
Size: 2093 345 5907
Fragments arranged by size:
5907 2093 345
BmrI ACTGGGnnnn_n'
Cuts at: 789 1759 2572 2713 3804 5199 5557 5638 5973
Size: 970 813 141 1091 1395 358 81 335
Cuts at: 5973 8117 789
Size: 2144 1017
Fragments arranged by size:
2144 1395 1091 1017 970 813 358 335
141 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 5262 6712 3143
Size: 2119 1450 4776
Fragments arranged by size:
4776 2119 1450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 352 1024 1090 1096 1133 1840 2401 2478
Size: 132 672 66 6 37 707 561 77
Cuts at: 2478 2674 3800 4189 4630 4933 5293 220
Size: 196 1126 389 441 303 360 3272
Fragments arranged by size:
3272 1126 707 672 561 441 389 360
303 196 132 77 66 37 6
Bpu10I CC'TnA_GC
Cuts at: 334 2539 4880 334
Size: 2205 2341 3799
Fragments arranged by size:
3799 2341 2205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3138 5521 6400 6892 7190 3138
Size: 2383 879 492 298 4293
Fragments arranged by size:
4293 2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1698 1751 2072 3220 4090 4648 5032 8049 1698
Size: 53 321 1148 870 558 384 3017 1994
Fragments arranged by size:
3017 1994 1148 870 558 384 321 53
BsaAI yAC'GTr
Cuts at: 2522 2712 4641 5698 2522
Size: 190 1929 1057 5169
Fragments arranged by size:
5169 1929 1057 190
BsaBI GATnn'nnATC
Cuts at: 6 5859 7896 6
Size: 5853 2037 455
Fragments arranged by size:
5853 2037 455
BsaHI Gr'CG_yC
Cuts at: 1070 3721 4471 4568 5776 6024 6726 8222 1070
Size: 2651 750 97 1208 248 702 1496 1193
Fragments arranged by size:
2651 1496 1208 1193 750 702 248 97
BsaWI w'CCGG_w
Cuts at: 1850 5534 6055 6733 7007 7154 7565 1850
Size: 3684 521 678 274 147 411 2630
Fragments arranged by size:
3684 2630 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2486 2516 2716 2746 2870 2900 3204 3234 3828
Size: 30 200 30 124 30 304 30 594
Cuts at: 3828 3858 4168 4198 4480 4510 2486
Size: 30 310 30 282 30 6321
Fragments arranged by size:
6321 594 310 304 282 200 124 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 349 403 501 1180 1544 2410 2947 3836 4090
Size: 54 98 679 364 866 537 889 254
Cuts at: 4090 4186 4189 4501 5251 349
Size: 96 3 312 750 3443
Fragments arranged by size:
3443 889 866 750 679 537 364 312
254 98 96 54 3
BseYI C'CCAG_C
Cuts at: 1296 1798 1908 2740 2759 3434 3661 3879 5291
Size: 502 110 832 19 675 227 218 1412
Cuts at: 5291 5770 6528 7105 7601 1296
Size: 479 758 577 496 2040
Fragments arranged by size:
2040 1412 832 758 675 577 502 496
479 227 218 110 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 955 2296 2596 3608 3901 4750 4774 5044
Size: 705 1341 300 1012 293 849 24 270
Cuts at: 5044 250
Size: 3551
Fragments arranged by size:
3551 1341 1012 849 705 300 293 270
24
BsiEI CG_ry'CG
Cuts at: 104 1540 5887 5933 7141 104
Size: 1436 4347 46 1208 1308
Fragments arranged by size:
4347 1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 619 1178 1826 3214 4508 5269 5359 6137 6327
Size: 559 648 1388 1294 761 90 778 190
Cuts at: 6327 7119 619
Size: 792 1845
Fragments arranged by size:
1845 1388 1294 792 778 761 648 559
190 90
BsmI GAATG_Cn'
Cuts at: 1307 1403 1307
Size: 96 8249
Bsp1286I G_dGCh'C
Cuts at: 248 377 527 619 965 1178 1254 1673 1826
Size: 129 150 92 346 213 76 419 153
Cuts at: 1826 2037 2300 2379 2785 2816 3214 3668 3678
Size: 211 263 79 406 31 398 454 10
Cuts at: 3678 3802 4508 4667 4824 4894 5269 5292 5359
Size: 124 706 159 157 70 375 23 67
Cuts at: 5359 5970 6063 6137 6327 6389 7119 248
Size: 611 93 74 190 62 730 1474
Fragments arranged by size:
1474 730 706 611 454 419 406 398
375 346 263 213 211 190 159 157
153 150 129 124 93 92 79 76
74 70 67 62 31 23 10
BspEI T'CCGG_A
Cuts at: 7565 7565
Size: 8345
BspHI T'CATG_A
Cuts at: 1658 7521 1658
Size: 5863 2482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 2618 2754 3372 3740 4294 5219 5395 5609
Size: 2109 136 618 368 554 925 176 214
Cuts at: 5609 5911 6292 509
Size: 302 381 2562
Fragments arranged by size:
2562 2109 925 618 554 381 368 302
214 176 136
BsrBI CCG'CTC
Cuts at: 50 1391 1536 4917 6637 6691 8175 8340 50
Size: 1341 145 3381 1720 54 1484 165 55
Fragments arranged by size:
3381 1720 1484 1341 165 145 55 54
BsrDI GCAATG_nn'
Cuts at: 3516 3770 3828 6257 3516
Size: 254 58 2429 5604
Fragments arranged by size:
5604 2429 254 58
BsrFI r'CCGG_y
Cuts at: 4740 4758 6343 6524 6733 4740
Size: 18 1585 181 209 6352
Fragments arranged by size:
6352 1585 209 181 18
BsrGI T'GTAC_A
Cuts at: 680 680
Size: 8345
BssSI C'ACGA_G
Cuts at: 1556 3931 6616 6974 1556
Size: 2375 2685 358 2927
Fragments arranged by size:
2927 2685 2375 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3924 4737 5287 5722 3924
Size: 813 550 435 6547
Fragments arranged by size:
6547 813 550 435
BstBI TT'CG_AA
Cuts at: 2864 6706 2864
Size: 3842 4503
BstXI CCAn_nnnn'nTGG
Cuts at: 743 2450 3778 3887 4482 5317 743
Size: 1707 1328 109 595 835 3771
Fragments arranged by size:
3771 1707 1328 835 595 109
BstYI r'GATC_y
Cuts at: 1 749 1215 2384 2528 3057 3246 3272 5370
Size: 748 466 1169 144 529 189 26 2098
Cuts at: 5370 5837 5860 6195 6441 7453 7539 7551 7897
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7897 1
Size: 449
Fragments arranged by size:
2098 1169 1012 748 529 467 466 449
346 335 246 189 144 86 26 23
12
Bsu36I CC'TnA_GG
Cuts at: 425 425
Size: 8345
BtgI C'CryG_G
Cuts at: 451 1328 1592 1752 2532 2816 3242 451
Size: 877 264 160 780 284 426 5554
Fragments arranged by size:
5554 877 780 426 284 264 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3531 4545 6295 6476 6639 3531
Size: 1014 1750 181 163 5237
Fragments arranged by size:
5237 1750 1014 181 163
BtsI GCAGTG_nn'
Cuts at: 1605 3211 3214 5588 1605
Size: 1606 3 2374 4362
Fragments arranged by size:
4362 2374 1606 3
DraI TTT'AAA
Cuts at: 5344 5344
Size: 8345
DraIII CAC_nnn'GTG
Cuts at: 1242 1767 3243 4517 4853 1242
Size: 525 1476 1274 336 4734
Fragments arranged by size:
4734 1476 1274 525 336
DrdI GACnn_nn'nnGTC
Cuts at: 2111 6051 6909 2111
Size: 3940 858 3547
Fragments arranged by size:
3940 3547 858
EaeI y'GGCC_r
Cuts at: 564 1392 1537 1769 2570 3566 4556 4760 4844
Size: 828 145 232 801 996 990 204 84
Cuts at: 4844 5930 6104 6495 6522 7867 564
Size: 1086 174 391 27 1345 1042
Fragments arranged by size:
1345 1086 1042 996 990 828 801 391
232 204 174 145 84 27
EagI C'GGCC_G
Cuts at: 1537 5930 1537
Size: 4393 3952
EarI CTCTTCn'nnn_
Cuts at: 385 511 2335 6368 6578 385
Size: 126 1824 4033 210 2152
Fragments arranged by size:
4033 2152 1824 210 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2608 2626 2762 3307 4542 5017 6553 6863 7009
Size: 18 136 545 1235 475 1536 310 146
Cuts at: 7009 8155 2608
Size: 1146 2798
Fragments arranged by size:
2798 1536 1235 1146 545 475 310 146
136 18
EcoICRI GAG'CTC
Cuts at: 1176 1824 5357 1176
Size: 648 3533 4164
Fragments arranged by size:
4164 3533 648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 352 1024 1090 1096 1133 1376 1490 1840
Size: 132 672 66 6 37 243 114 350
Cuts at: 1840 2023 2401 2478 2674 2860 3800 4189 4261
Size: 183 378 77 196 186 940 389 72
Cuts at: 4261 4630 4883 4933 5293 6170 6602 7349 7617
Size: 369 253 50 360 877 432 747 268
Cuts at: 7617 220
Size: 948
Fragments arranged by size:
948 940 877 747 672 432 389 378
369 360 350 268 253 243 196 186
183 132 114 77 72 66 50 37
6
EcoNI CCTnn'n_nnAGG
Cuts at: 970 1051 1264 1508 2392 4171 970
Size: 81 213 244 884 1779 5144
Fragments arranged by size:
5144 1779 884 244 213 81
EcoO109I rG'GnC_Cy
Cuts at: 210 421 740 1250 1251 1513 1679 1802 2400
Size: 211 319 510 1 262 166 123 598
Cuts at: 2400 2858 3086 3360 3462 3876 4245 4293 4663
Size: 458 228 274 102 414 369 48 370
Cuts at: 4663 4664 5484 210
Size: 1 820 3071
Fragments arranged by size:
3071 820 598 510 458 414 370 369
319 274 262 228 211 166 123 102
48 1 1
EcoRI G'AATT_C
Cuts at: 2866 5349 2866
Size: 2483 5862
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 1008 1893 6364 342
Size: 666 885 4471 2323
Fragments arranged by size:
4471 2323 885 666
FspI TGC'GCA
Cuts at: 2335 2431 5101 6126 2335
Size: 96 2670 1025 4554
Fragments arranged by size:
4554 2670 1025 96
HaeII r_GCGC'y
Cuts at: 1909 2211 3724 4474 5779 6027 7049 8028 1909
Size: 302 1513 750 1305 248 1022 979 2226
Fragments arranged by size:
2226 1513 1305 1022 979 750 302 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 1165 1197 1634 1666 1775 1807 2872
Size: 32 679 32 437 32 109 32 1065
Cuts at: 2872 2904 4027 4059 4480 4512 5151 5183 6187
Size: 32 1123 32 421 32 639 32 1004
Cuts at: 6187 6217 6219 6249 454
Size: 30 2 30 2550
Fragments arranged by size:
2550 1123 1065 1004 679 639 437 421
109 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 5384 5384
Size: 8345
KasI G'GCGC_C
Cuts at: 3720 4470 5775 6023 3720
Size: 750 1305 248 6042
Fragments arranged by size:
6042 1305 750 248
KpnI G_GTAC'C
Cuts at: 582 5365 582
Size: 4783 3562
MluI A'CGCG_T
Cuts at: 7778 7778
Size: 8345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 806 2394 3190 3884 4426 7016 7200 806
Size: 1588 796 694 542 2590 184 1951
Fragments arranged by size:
2590 1951 1588 796 694 542 184
MscI TGG'CCA
Cuts at: 566 1771 2572 4558 4846 6106 566
Size: 1205 801 1986 288 1260 2805
Fragments arranged by size:
2805 1986 1260 1205 801 288
MslI CAynn'nnrTG
Cuts at: 634 884 2425 3763 6334 6461 634
Size: 250 1541 1338 2571 127 2518
Fragments arranged by size:
2571 2518 1541 1338 250 127
MspA1I CmG'CkG
Cuts at: 435 570 2056 2262 2652 2830 3685 4143 4434
Size: 135 1486 206 390 178 855 458 291
Cuts at: 4434 5086 5277 5456 5770 6130 7143 7388 435
Size: 652 191 179 314 360 1013 245 1392
Fragments arranged by size:
1486 1392 1013 855 652 458 390 360
314 291 245 206 191 179 178 135
NaeI GCC'GGC
Cuts at: 4760 6526 4760
Size: 1766 6579
NarI GG'CG_CC
Cuts at: 3721 4471 5776 6024 3721
Size: 750 1305 248 6042
Fragments arranged by size:
6042 1305 750 248
NcoI C'CATG_G
Cuts at: 451 1752 2532 451
Size: 1301 780 6264
Fragments arranged by size:
6264 1301 780
NdeI CA'TA_TG
Cuts at: 948 948
Size: 8345
NgoMIV G'CCGG_C
Cuts at: 4758 6524 4758
Size: 1766 6579
NspI r_CATG'y
Cuts at: 687 3270 3492 5398 5898 6805 687
Size: 2583 222 1906 500 907 2227
Fragments arranged by size:
2583 2227 1906 907 500 222
PciI A'CATG_T
Cuts at: 6801 6801
Size: 8345
PflMI CCAn_nnn'nTGG
Cuts at: 438 1303 1716 1767 3243 438
Size: 865 413 51 1476 5540
Fragments arranged by size:
5540 1476 865 413 51
PfoI T'CCnGG_A
Cuts at: 236 701 1064 1361 3458 3575 4265 5309 236
Size: 465 363 297 2097 117 690 1044 3272
Fragments arranged by size:
3272 2097 1044 690 465 363 297 117
PmeI GTTT'AAAC
Cuts at: 5344 5344
Size: 8345
PmlI CAC'GTG
Cuts at: 2522 2712 2522
Size: 190 8155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7484 7516 7484
Size: 32 8313
PpuMI rG'GwC_Cy
Cuts at: 210 740 1679 2400 2858 3360 3462 4245 4293
Size: 530 939 721 458 502 102 783 48
Cuts at: 4293 210
Size: 4262
Fragments arranged by size:
4262 939 783 721 530 502 458 102
48
PshAI GACnn'nnGTC
Cuts at: 1690 3467 1690
Size: 1777 6568
PspOMI G'GGCC_C
Cuts at: 1250 4663 1250
Size: 3413 4932
PspXI vC'TCGA_Gb
Cuts at: 2852 3128 5191 2852
Size: 276 2063 6006
Fragments arranged by size:
6006 2063 276
PstI C_TGCA'G
Cuts at: 164 1134 1268 1442 1458 1941 2393 2903 3410
Size: 970 134 174 16 483 452 510 507
Cuts at: 3410 4142 4184 4415 4433 4928 5392 6077 164
Size: 732 42 231 18 495 464 685 2432
Fragments arranged by size:
2432 970 732 685 510 507 495 483
464 452 231 174 134 42 18 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8345
PvuII CAG'CTG
Cuts at: 435 2652 3685 4143 4434 5770 6130 435
Size: 2217 1033 458 291 1336 360 2650
Fragments arranged by size:
2650 2217 1336 1033 458 360 291
RsrII CG'GwC_CG
Cuts at: 6540 6540
Size: 8345
SacI G_AGCT'C
Cuts at: 1178 1826 5359 1178
Size: 648 3533 4164
Fragments arranged by size:
4164 3533 648
SalI G'TCGA_C
Cuts at: 5382 5382
Size: 8345
SanDI GG'GwC_CC
Cuts at: 210 210
Size: 8345
SapI GCTCTTCn'nnn_
Cuts at: 385 511 2335 6368 6578 385
Size: 126 1824 4033 210 2152
Fragments arranged by size:
4033 2152 1824 210 126
SbfI CC_TGCA'GG
Cuts at: 2393 5392 2393
Size: 2999 5346
ScaI AGT'ACT
Cuts at: 7862 7862
Size: 8345
SfcI C'TryA_G
Cuts at: 33 160 1130 1264 1438 1454 1937 2389 2899
Size: 127 970 134 174 16 483 452 510
Cuts at: 2899 3406 4138 4180 4297 4411 4429 4924 4941
Size: 507 732 42 117 114 18 495 17
Cuts at: 4941 5388 6073 7066 7257 33
Size: 447 685 993 191 1121
Fragments arranged by size:
1121 993 970 732 685 510 507 495
483 452 447 191 174 134 127 117
114 42 18 17 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 2363 2363
Size: 8345
SfoI GGC'GCC
Cuts at: 3722 4472 5777 6025 3722
Size: 750 1305 248 6042
Fragments arranged by size:
6042 1305 750 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8345
SmaI CCC'GGG
Cuts at: 1518 4705 5367 1518
Size: 3187 662 4496
Fragments arranged by size:
4496 3187 662
SmlI C'TyrA_G
Cuts at: 2852 3117 3128 5191 5500 6415 6907 7169 2852
Size: 265 11 2063 309 915 492 262 4028
Fragments arranged by size:
4028 2063 915 492 309 265 262 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8345
SphI G_CATG'C
Cuts at: 3492 5398 5898 3492
Size: 1906 500 5939
Fragments arranged by size:
5939 1906 500
SspI AAT'ATT
Cuts at: 2489 5155 2489
Size: 2666 5679
StuI AGG'CCT
Cuts at: 1270 2561 1270
Size: 1291 7054
StyI C'CwwG_G
Cuts at: 451 1211 1271 1752 1916 2117 2532 2891 2957
Size: 760 60 481 164 201 415 359 66
Cuts at: 2957 2978 3230 3716 3993 4190 4388 4475 5069
Size: 21 252 486 277 197 198 87 594
Cuts at: 5069 5479 5833 451
Size: 410 354 2963
Fragments arranged by size:
2963 760 594 486 481 415 410 359
354 277 252 201 198 197 164 87
66 60 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 808 1680 1778 3661 4672 4680 5065 5928 6061
Size: 872 98 1883 1011 8 385 863 133
Cuts at: 6061 6520 6729 808
Size: 459 209 2424
Fragments arranged by size:
2424 1883 1011 872 863 459 385 209
133 98 8
TatI w'GTAC_w
Cuts at: 680 3791 4331 7860 680
Size: 3111 540 3529 1165
Fragments arranged by size:
3529 3111 1165 540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 245 277 2486 2518 2716 2748 2870 2902 3204
Size: 32 2209 32 198 32 122 32 302
Cuts at: 3204 3236 3828 3860 7862 7894 245
Size: 32 592 32 4002 32 696
Fragments arranged by size:
4002 2209 696 592 302 198 122 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4690 6142 4690
Size: 1452 6893
XbaI T'CTAG_A
Cuts at: 5376 5376
Size: 8345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 633 1488 3156 3723 5840 633
Size: 855 1668 567 2117 3138
Fragments arranged by size:
3138 2117 1668 855 567
XhoI C'TCGA_G
Cuts at: 2852 3128 5191 2852
Size: 276 2063 6006
Fragments arranged by size:
6006 2063 276
XmaI C'CCGG_G
Cuts at: 1516 4703 5365 1516
Size: 3187 662 4496
Fragments arranged by size:
4496 3187 662
XmnI GAAnn'nnTTC
Cuts at: 988 4120 988
Size: 3132 5213
ZraI GAC'GTC
Cuts at: 4569 4569
Size: 8345
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AleI AlfI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PsiI PsrI SacII SexAI SgrAI
SnaBI SrfI SwaI