Restriction map for
pF1KB1311
(Circular) (Six-base) MAPSORT of: pF1KB1311.seq Check: 326 from: 1 to: 7265
pF1KB1311 7265 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 809 809
Size: 7265
AccI GT'mk_AC
Cuts at: 3335 4303 3335
Size: 968 6297
Acc65I G'GTAC_C
Cuts at: 1182 1415 2962 3275 4281 1182
Size: 233 1547 313 1006 4166
Fragments arranged by size:
4166 1547 1006 313 233
AclI AA'CG_TT
Cuts at: 3341 7105 3341
Size: 3764 3501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 693 1384 1951 2529 3348 3354 3445 4100 4277
Size: 691 567 578 819 6 91 655 177
Cuts at: 4277 5090 5522 6269 6537 693
Size: 813 432 747 268 1421
Fragments arranged by size:
1421 819 813 747 691 655 578 567
432 268 177 91 6
AfeI AGC'GCT
Cuts at: 961 2248 3037 3155 3706 961
Size: 1287 789 118 551 4520
Fragments arranged by size:
4520 1287 789 551 118
AflII C'TTAA_G
Cuts at: 2872 2872
Size: 7265
AflIII A'CryG_T
Cuts at: 400 698 1367 1717 1748 3050 3052 3494 4617
Size: 298 669 350 31 1302 2 442 1123
Cuts at: 4617 5721 6698 400
Size: 1104 977 967
Fragments arranged by size:
1302 1123 1104 977 967 669 442 350
298 31 2
AgeI A'CCGG_T
Cuts at: 5653 5653
Size: 7265
AleI CACnn'nnGTG
Cuts at: 859 859
Size: 7265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1648 3148 1648
Size: 1500 5765
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 2455
Size: 32 7233
AlwNI CAG_nnn'CTG
Cuts at: 4123 4207 4239 4570 6137 4123
Size: 84 32 331 1567 5251
Fragments arranged by size:
5251 1567 331 84 32
ApaI G_GGCC'C
Cuts at: 2593 2593
Size: 7265
ApaLI G'TGCA_C
Cuts at: 6035 6035
Size: 7265
ApoI r'AATT_y
Cuts at: 933 1449 2454 4269 6618 6874 933
Size: 516 1005 1815 2349 256 1324
Fragments arranged by size:
2349 1815 1324 1005 516 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7265
AvaI C'yCGr_G
Cuts at: 781 2009 2562 2642 3228 3271 4214 4285 781
Size: 1228 553 80 586 43 943 71 3761
Fragments arranged by size:
3761 1228 943 586 553 80 71 43
BamHI G'GATC_C
Cuts at: 306 350 2240 4290 306
Size: 44 1890 2050 3281
Fragments arranged by size:
3281 2050 1890 44
BanI G'GyrC_C
Cuts at: 223 757 1030 1127 1182 1415 2606 2732 2962
Size: 534 273 97 55 233 1191 126 230
Cuts at: 2962 3124 3275 3481 3885 4281 4695 4943 4978
Size: 162 151 206 404 396 414 248 35
Cuts at: 4978 223
Size: 2510
Fragments arranged by size:
2510 1191 534 414 404 396 273 248
233 230 206 162 151 126 97 55
35
BanII G_rGCy'C
Cuts at: 376 2593 4048 4067 4279 5309 376
Size: 2217 1455 19 212 1030 2332
Fragments arranged by size:
2332 2217 1455 1030 212 19
BbeI G_GCGC'C
Cuts at: 227 761 1034 1131 2610 4699 4947 227
Size: 534 273 97 1479 2089 248 2545
Fragments arranged by size:
2545 2089 1479 534 273 248 97
BbsI GAAGACnn'nnnn_
Cuts at: 730 2562 2646 730
Size: 1832 84 5349
Fragments arranged by size:
5349 1832 84
BbvCI CC'TCA_GC
Cuts at: 1700 3815 4170 1700
Size: 2115 355 4795
Fragments arranged by size:
4795 2115 355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 120 154 1574 1608 4015 4049 120
Size: 34 1420 34 2407 34 3336
Fragments arranged by size:
3336 2407 1420 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2382 5163 5924 7265 2382
Size: 2781 761 1341 2382
Fragments arranged by size:
2781 2382 1341 761
BclI T'GATC_A
Cuts at: 4785 4785
Size: 7265
BfrBI ATG'CAT
Cuts at: 114 2068 114
Size: 1954 5311
BglI GCCn_nnn'nGGC
Cuts at: 605 3306 605
Size: 2701 4564
BglII A'GATC_T
Cuts at: 4780 6817 4780
Size: 2037 5228
BlpI GC'TnA_GC
Cuts at: 1348 3190 3806 4377 1348
Size: 1842 616 571 4236
Fragments arranged by size:
4236 1842 616 571
Bme1580I G_kGCm'C
Cuts at: 464 557 798 1232 2091 2593 2994 3127 3317
Size: 93 241 434 859 502 401 133 190
Cuts at: 3317 3484 4890 4983 6039 464
Size: 167 1406 93 1056 1690
Fragments arranged by size:
1690 1406 1056 859 502 434 401 241
190 167 133 93 93
BmgBI CAC'GTC
Cuts at: 699 3871 699
Size: 3172 4093
BmrI ACTGGGnnnn_n'
Cuts at: 402 2229 2313 2409 4477 4558 4893 7037 402
Size: 1827 84 96 2068 81 335 2144 630
Fragments arranged by size:
2144 2068 1827 630 335 96 84 81
BmtI G_CTAG'C
Cuts at: 3814 3814
Size: 7265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2982 3375 5632 2982
Size: 393 2257 4615
Fragments arranged by size:
4615 2257 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 808 2956 4148 4178 4261 307
Size: 501 2148 1192 30 83 3311
Fragments arranged by size:
3311 2148 1192 501 83 30
Bpu10I CC'TnA_GC
Cuts at: 1700 3815 3893 4170 1700
Size: 2115 78 277 4795
Fragments arranged by size:
4795 2115 277 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 2919 3754 4441 5320 5812 6110 1621
Size: 1298 835 687 879 492 298 2776
Fragments arranged by size:
2776 1298 879 835 687 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 1521 1822 3363 6969 153
Size: 1368 301 1541 3606 449
Fragments arranged by size:
3606 1541 1368 449 301
BsaAI yAC'GTr
Cuts at: 774 1335 3053 3495 4618 774
Size: 561 1718 442 1123 3421
Fragments arranged by size:
3421 1718 1123 561 442
BsaBI GATnn'nnATC
Cuts at: 6 311 1012 4779 6816 6
Size: 305 701 3767 2037 455
Fragments arranged by size:
3767 2037 701 455 305
BsaHI Gr'CG_yC
Cuts at: 224 758 1031 1128 2607 2880 4696 4944 5646
Size: 534 273 97 1479 273 1816 248 702
Cuts at: 5646 7142 224
Size: 1496 347
Fragments arranged by size:
1816 1496 1479 702 534 347 273 273
248 97
BsaWI w'CCGG_w
Cuts at: 347 1535 2267 3563 4454 4975 5653 5927 6074
Size: 1188 732 1296 891 521 678 274 147
Cuts at: 6074 6485 347
Size: 411 1127
Fragments arranged by size:
1296 1188 1127 891 732 678 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1010 1040 1582 1612 2541 2571 3341 3371 4134
Size: 30 542 30 929 30 770 30 763
Cuts at: 4134 4164 4237 4267 1010
Size: 30 73 30 4008
Fragments arranged by size:
4008 929 770 763 542 73 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 219 739 2218 3367 4258 202
Size: 17 520 1479 1149 891 3209
Fragments arranged by size:
3209 1479 1149 891 520 17
BseYI C'CCAG_C
Cuts at: 164 1326 1411 2105 2231 3477 3990 4176 4182
Size: 1162 85 694 126 1246 513 186 6
Cuts at: 4182 4200 4690 5448 6025 6521 164
Size: 18 490 758 577 496 908
Fragments arranged by size:
1246 1162 908 758 694 577 513 496
490 186 126 85 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 502 730 793 976 1758 2474 2961 256
Size: 246 228 63 183 782 716 487 4560
Fragments arranged by size:
4560 782 716 487 246 228 183 63
BsiEI CG_ry'CG
Cuts at: 104 426 1389 2451 4807 4853 6061 104
Size: 322 963 1062 2356 46 1208 1308
Fragments arranged by size:
2356 1308 1208 1062 963 322 46
BsiHKAI G_wGCw'C
Cuts at: 3440 4016 4279 5057 5247 6039 3440
Size: 576 263 778 190 792 4666
Fragments arranged by size:
4666 792 778 576 263 190
BsmI GAATG_Cn'
Cuts at: 1612 2070 1612
Size: 458 6807
BsmBI CGTCTCn'nnnn_
Cuts at: 2871 2871
Size: 7265
Bsp1286I G_dGCh'C
Cuts at: 376 464 557 798 1232 2091 2593 2994 3127
Size: 88 93 241 434 859 502 401 133
Cuts at: 3127 3317 3440 3484 4016 4048 4067 4279 4890
Size: 190 123 44 532 32 19 212 611
Cuts at: 4890 4983 5057 5247 5309 6039 376
Size: 93 74 190 62 730 1602
Fragments arranged by size:
1602 859 730 611 532 502 434 401
241 212 190 190 133 123 93 93
88 74 62 44 32 19
BspEI T'CCGG_A
Cuts at: 1535 6485 1535
Size: 4950 2315
BspHI T'CATG_A
Cuts at: 6441 6441
Size: 7265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 524 809 1350 1840 4315 4529 4831 5212 524
Size: 285 541 490 2475 214 302 381 2577
Fragments arranged by size:
2577 2475 541 490 381 302 285 214
BsrBI CCG'CTC
Cuts at: 50 1385 2034 2399 5557 5611 7095 7260 50
Size: 1335 649 365 3158 54 1484 165 55
Fragments arranged by size:
3158 1484 1335 649 365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5177 5177
Size: 7265
BsrFI r'CCGG_y
Cuts at: 1679 2786 3194 5263 5444 5653 1679
Size: 1107 408 2069 181 209 3291
Fragments arranged by size:
3291 2069 1107 408 209 181
BssHII G'CGCG_C
Cuts at: 535 535
Size: 7265
BssSI C'ACGA_G
Cuts at: 671 1441 4078 5536 5894 671
Size: 770 2637 1458 358 2042
Fragments arranged by size:
2637 2042 1458 770 358
BstAPI GCAn_nnn'nTGC
Cuts at: 903 4642 903
Size: 3739 3526
BstBI TT'CG_AA
Cuts at: 5626 5626
Size: 7265
BstEII G'GTnAC_C
Cuts at: 290 3319 290
Size: 3029 4236
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 7265
BstYI r'GATC_y
Cuts at: 1 306 350 2240 2860 3698 4235 4290 4757
Size: 305 44 1890 620 838 537 55 467
Cuts at: 4757 4780 5115 5361 6373 6459 6471 6817 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1890 1012 838 620 537 467 449 346
335 305 246 86 55 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 589 2425 589
Size: 1836 5429
BtgI C'CryG_G
Cuts at: 143 295 359 1068 1245 1617 3015 3264 3324
Size: 152 64 709 177 372 1398 249 60
Cuts at: 3324 143
Size: 4084
Fragments arranged by size:
4084 1398 709 372 249 177 152 64
60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 941 1092 1359 4018 5215 5396 5559 941
Size: 151 267 2659 1197 181 163 2647
Fragments arranged by size:
2659 2647 1197 267 181 163 151
BtsI GCAGTG_nn'
Cuts at: 530 1926 2159 2812 4508 530
Size: 1396 233 653 1696 3287
Fragments arranged by size:
3287 1696 1396 653 233
DraI TTT'AAA
Cuts at: 4264 4264
Size: 7265
DraIII CAC_nnn'GTG
Cuts at: 903 1246 1446 903
Size: 343 200 6722
Fragments arranged by size:
6722 343 200
DrdI GACnn_nn'nnGTC
Cuts at: 4971 5829 4971
Size: 858 6407
EaeI y'GGCC_r
Cuts at: 362 921 1118 1248 1386 2035 2193 3196 3528
Size: 559 197 130 138 649 158 1003 332
Cuts at: 3528 4850 5024 5415 5442 6787 362
Size: 1322 174 391 27 1345 840
Fragments arranged by size:
1345 1322 1003 840 649 559 391 332
197 174 158 138 130 27
EagI C'GGCC_G
Cuts at: 1386 4850 1386
Size: 3464 3801
EarI CTCTTCn'nnn_
Cuts at: 130 3577 4021 5288 5498 130
Size: 3447 444 1267 210 1897
Fragments arranged by size:
3447 1897 1267 444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 887 3372 5473 5783 5929 7075 887
Size: 2485 2101 310 146 1146 1077
Fragments arranged by size:
2485 2101 1146 1077 310 146
EcoICRI GAG'CTC
Cuts at: 4277 4277
Size: 7265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 693 808 1384 1951 2529 2956 3348 3354
Size: 386 115 576 567 578 427 392 6
Cuts at: 3354 3445 4100 4148 4178 4261 4277 5090 5522
Size: 91 655 48 30 83 16 813 432
Cuts at: 5522 6269 6537 307
Size: 747 268 1035
Fragments arranged by size:
1035 813 747 655 578 576 567 432
427 392 386 268 115 91 83 48
30 16 6
EcoO109I rG'GnC_Cy
Cuts at: 199 1207 1284 1544 1704 2549 2589 2590 3455
Size: 1008 77 260 160 845 40 1 865
Cuts at: 3455 3819 4120 4131 4404 199
Size: 364 301 11 273 3060
Fragments arranged by size:
3060 1008 865 845 364 301 273 260
160 77 40 11 1
EcoRI G'AATT_C
Cuts at: 4269 4269
Size: 7265
EcoRV GAT'ATC
Cuts at: 1956 3472 1956
Size: 1516 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3795 5284 3795
Size: 1489 5776
FspI TGC'GCA
Cuts at: 5046 5046
Size: 7265
HaeII r_GCGC'y
Cuts at: 227 761 963 1034 1131 2250 2610 3039 3157
Size: 534 202 71 97 1119 360 429 118
Cuts at: 3157 3708 4699 4947 5969 6948 227
Size: 551 991 248 1022 979 544
Fragments arranged by size:
1119 1022 991 979 551 544 534 429
360 248 202 118 97 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 640 672 1582 1614 2541 2573 3341
Size: 32 317 32 910 32 927 32 768
Cuts at: 3341 3373 4237 4269 5107 5137 5139 5169 291
Size: 32 864 32 838 30 2 30 2387
Fragments arranged by size:
2387 927 910 864 838 768 317 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1104 1365 1651 1659 4304 1104
Size: 261 286 8 2645 4065
Fragments arranged by size:
4065 2645 286 261 8
HindIII A'AGCT_T
Cuts at: 1396 1396
Size: 7265
KasI G'GCGC_C
Cuts at: 223 757 1030 1127 2606 4695 4943 223
Size: 534 273 97 1479 2089 248 2545
Fragments arranged by size:
2545 2089 1479 534 273 248 97
KpnI G_GTAC'C
Cuts at: 1186 1419 2966 3279 4285 1186
Size: 233 1547 313 1006 4166
Fragments arranged by size:
4166 1547 1006 313 233
MluI A'CGCG_T
Cuts at: 6698 6698
Size: 7265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1625 3617 5936 6120 1625
Size: 1992 2319 184 2770
Fragments arranged by size:
2770 2319 1992 184
MscI TGG'CCA
Cuts at: 364 3530 5026 364
Size: 3166 1496 2603
Fragments arranged by size:
3166 2603 1496
MslI CAynn'nnrTG
Cuts at: 859 1006 2830 3130 3433 3580 4058 5254 5381
Size: 147 1824 300 303 147 478 1196 127
Cuts at: 5381 859
Size: 2743
Fragments arranged by size:
2743 1824 1196 478 303 300 147 147
127
MspA1I CmG'CkG
Cuts at: 145 437 1070 3026 3465 3477 4376 4690 5050
Size: 292 633 1956 439 12 899 314 360
Cuts at: 5050 6063 6308 145
Size: 1013 245 1102
Fragments arranged by size:
1956 1102 1013 899 633 439 360 314
292 245 12
NaeI GCC'GGC
Cuts at: 1681 3196 5446 1681
Size: 1515 2250 3500
Fragments arranged by size:
3500 2250 1515
NarI GG'CG_CC
Cuts at: 224 758 1031 1128 2607 4696 4944 224
Size: 534 273 97 1479 2089 248 2545
Fragments arranged by size:
2545 2089 1479 534 273 248 97
NcoI C'CATG_G
Cuts at: 1617 3015 3264 1617
Size: 1398 249 5618
Fragments arranged by size:
5618 1398 249
NgoMIV G'CCGG_C
Cuts at: 1679 3194 5444 1679
Size: 1515 2250 3500
Fragments arranged by size:
3500 2250 1515
NheI G'CTAG_C
Cuts at: 3810 3810
Size: 7265
NsiI A_TGCA'T
Cuts at: 116 2070 116
Size: 1954 5311
NspI r_CATG'y
Cuts at: 411 1721 1752 3189 4318 4818 5725 411
Size: 1310 31 1437 1129 500 907 1951
Fragments arranged by size:
1951 1437 1310 1129 907 500 31
PasI CC'CwG_GG
Cuts at: 219 762 2792 219
Size: 543 2030 4692
Fragments arranged by size:
4692 2030 543
PciI A'CATG_T
Cuts at: 1717 1748 5721 1717
Size: 31 3973 3261
Fragments arranged by size:
3973 3261 31
PflMI CCAn_nnn'nTGG
Cuts at: 301 1246 1586 2526 4207 4239 301
Size: 945 340 940 1681 32 3327
Fragments arranged by size:
3327 1681 945 940 340 32
PfoI T'CCnGG_A
Cuts at: 1135 2458 4238 1135
Size: 1323 1780 4162
Fragments arranged by size:
4162 1780 1323
PmeI GTTT'AAAC
Cuts at: 4264 4264
Size: 7265
PmlI CAC'GTG
Cuts at: 1335 3053 1335
Size: 1718 5547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2433 3595 3627 6404 6436 2401
Size: 32 1162 32 2777 32 3230
Fragments arranged by size:
3230 2777 1162 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 199 1284 1544 1704 2549 3455 4131 199
Size: 1085 260 160 845 906 676 3333
Fragments arranged by size:
3333 1085 906 845 676 260 160
PshAI GACnn'nnGTC
Cuts at: 3682 3682
Size: 7265
PspOMI G'GGCC_C
Cuts at: 2589 2589
Size: 7265
PspXI vC'TCGA_Gb
Cuts at: 781 2009 781
Size: 1228 6037
PstI C_TGCA'G
Cuts at: 2166 2819 2868 3168 4312 4997 2166
Size: 653 49 300 1144 685 4434
Fragments arranged by size:
4434 1144 685 653 300 49
PvuI CG_AT'CG
Cuts at: 104 2451 104
Size: 2347 4918
PvuII CAG'CTG
Cuts at: 437 3465 3477 4690 5050 437
Size: 3028 12 1213 360 2652
Fragments arranged by size:
3028 2652 1213 360 12
RsrII CG'GwC_CG
Cuts at: 5460 5460
Size: 7265
SacI G_AGCT'C
Cuts at: 4279 4279
Size: 7265
SacII CC_GC'GG
Cuts at: 146 1071 146
Size: 925 6340
SalI G'TCGA_C
Cuts at: 4302 4302
Size: 7265
SanDI GG'GwC_CC
Cuts at: 199 3455 199
Size: 3256 4009
SapI GCTCTTCn'nnn_
Cuts at: 130 4021 5288 5498 130
Size: 3891 1267 210 1897
Fragments arranged by size:
3891 1897 1267 210
SbfI CC_TGCA'GG
Cuts at: 4312 4312
Size: 7265
ScaI AGT'ACT
Cuts at: 2059 6782 2059
Size: 4723 2542
SexAI A'CCwGG_T
Cuts at: 846 2735 846
Size: 1889 5376
SfcI C'TryA_G
Cuts at: 33 237 2162 2178 2815 2864 3039 3145 3164
Size: 204 1925 16 637 49 175 106 19
Cuts at: 3164 4142 4308 4993 5986 6177 33
Size: 978 166 685 993 191 1121
Fragments arranged by size:
1925 1121 993 978 685 637 204 191
175 166 106 49 19 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 3306 3306
Size: 7265
SfoI GGC'GCC
Cuts at: 225 759 1032 1129 2608 4697 4945 225
Size: 534 273 97 1479 2089 248 2545
Fragments arranged by size:
2545 2089 1479 534 273 248 97
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7265
SmaI CCC'GGG
Cuts at: 2644 3230 3273 4216 4287 2644
Size: 586 43 943 71 5622
Fragments arranged by size:
5622 943 586 71 43
SmlI C'TyrA_G
Cuts at: 781 1636 2009 2872 2898 3769 4420 5335 5827
Size: 855 373 863 26 871 651 915 492
Cuts at: 5827 6089 781
Size: 262 1957
Fragments arranged by size:
1957 915 871 863 855 651 492 373
262 26
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7265
SphI G_CATG'C
Cuts at: 4318 4818 4318
Size: 500 6765
StuI AGG'CCT
Cuts at: 2430 3652 2430
Size: 1222 6043
StyI C'CwwG_G
Cuts at: 1617 3015 3264 3647 4067 4399 4753 1617
Size: 1398 249 383 420 332 354 4129
Fragments arranged by size:
4129 1398 420 383 354 332 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 440 736 929 2985 3538 3660 4848 4981 5440
Size: 296 193 2056 553 122 1188 133 459
Cuts at: 5440 5649 440
Size: 209 2056
Fragments arranged by size:
2056 2056 1188 553 459 296 209 193
133 122
TatI w'GTAC_w
Cuts at: 1946 2057 2291 2694 6780 1946
Size: 111 234 403 4086 2431
Fragments arranged by size:
4086 2431 403 234 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1010 1042 1893 1925 2483 2515 3995 4027 6782
Size: 32 851 32 558 32 1480 32 2755
Cuts at: 6782 6814 1010
Size: 32 1461
Fragments arranged by size:
2755 1480 1461 851 558 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 394 848 5062 394
Size: 454 4214 2597
Fragments arranged by size:
4214 2597 454
XbaI T'CTAG_A
Cuts at: 4296 4296
Size: 7265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 2523 3103 4760 1583
Size: 940 580 1657 4088
Fragments arranged by size:
4088 1657 940 580
XhoI C'TCGA_G
Cuts at: 781 2009 781
Size: 1228 6037
XmaI C'CCGG_G
Cuts at: 2642 3228 3271 4214 4285 2642
Size: 586 43 943 71 5622
Fragments arranged by size:
5622 943 586 71 43
XmnI GAAnn'nnTTC
Cuts at: 3571 3571
Size: 7265
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AhdI AscI AvrII BaeI BsiWI BsrGI BstZ17I
ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI
NotI NruI PacI PsiI PsrI SgrAI SnaBI SrfI
SspI SwaI ZraI