Restriction map for
pF1KB0996
(Circular) (Six-base) MAPSORT of: pF1KB0996.seq Check: 5988 from: 1 to: 4508
pF1KB0996 4508 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 160 160
Size: 4508
AccI GT'mk_AC
Cuts at: 738 1546 738
Size: 808 3700
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 790 2333 2765 3512 3780 568
Size: 222 1543 432 747 268 1296
Fragments arranged by size:
1543 1296 747 432 268 222
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AlwNI CAG_nnn'CTG
Cuts at: 212 582 732 901 1335 1813 3380 212
Size: 370 150 169 434 478 1567 1340
Fragments arranged by size:
1567 1340 478 434 370 169 150
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1346 1512 3861 4117 1346
Size: 166 2349 256 1737
Fragments arranged by size:
2349 1737 256 166
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 1528 1528
Size: 4508
BamHI G'GATC_C
Cuts at: 996 1533 996
Size: 537 3971
BanI G'GyrC_C
Cuts at: 459 1524 1938 2186 2221 459
Size: 1065 414 248 35 2746
Fragments arranged by size:
2746 1065 414 248 35
BanII G_rGCy'C
Cuts at: 300 826 1522 2552 300
Size: 526 696 1030 2256
Fragments arranged by size:
2256 1030 696 526
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BbsI GAAGACnn'nnnn_
Cuts at: 503 566 1149 503
Size: 63 583 3862
Fragments arranged by size:
3862 583 63
BciVI GTATCCnnnnn_n'
Cuts at: 1051 1386 2406 3167 4508 1051
Size: 335 1020 761 1341 1051
Fragments arranged by size:
1341 1051 1020 761 335
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 825 1620 825
Size: 795 3713
Bme1580I G_kGCm'C
Cuts at: 462 479 1222 2133 2226 3282 462
Size: 17 743 911 93 1056 1688
Fragments arranged by size:
1688 1056 911 743 93 17
BmrI ACTGGGnnnn_n'
Cuts at: 178 282 1720 1801 2136 4280 178
Size: 104 1438 81 335 2144 406
Fragments arranged by size:
2144 1438 406 335 104 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 921 2875 921
Size: 1954 2554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 649 723 730 826 511
Size: 138 74 7 96 4193
Fragments arranged by size:
4193 138 96 74 7
Bpu10I CC'TnA_GC
Cuts at: 166 166
Size: 4508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1684 2563 3055 3353 769
Size: 915 879 492 298 1924
Fragments arranged by size:
1924 915 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4212 4212
Size: 4508
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 1939 2187 2889 4385 1939
Size: 248 702 1496 2062
Fragments arranged by size:
2062 1496 702 248
BsaWI w'CCGG_w
Cuts at: 644 1177 1697 2218 2896 3170 3317 3728 644
Size: 533 520 521 678 274 147 411 1424
Fragments arranged by size:
1424 678 533 521 520 411 274 147
BseYI C'CCAG_C
Cuts at: 336 1933 2691 3268 3764 336
Size: 1597 758 577 496 1080
Fragments arranged by size:
1597 1080 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1231 1231
Size: 4508
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1522 2300 2490 3282 1522
Size: 778 190 792 2748
Fragments arranged by size:
2748 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 358 358
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 300 462 479 826 1222 1522 2133 2226 2300
Size: 162 17 347 396 300 611 93 74
Cuts at: 2300 2490 2552 3282 300
Size: 190 62 730 1526
Fragments arranged by size:
1526 730 611 396 347 300 190 162
93 74 62 17
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 1558 1772 2074 2455 160
Size: 1398 214 302 381 2213
Fragments arranged by size:
2213 1398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 456 2800 2854 4338 4503 50
Size: 406 2344 54 1484 165 55
Fragments arranged by size:
2344 1484 406 165 55 54
BsrDI GCAATG_nn'
Cuts at: 599 783 936 2420 599
Size: 184 153 1484 2687
Fragments arranged by size:
2687 1484 184 153
BsrFI r'CCGG_y
Cuts at: 2506 2687 2896 2506
Size: 181 209 4118
Fragments arranged by size:
4118 209 181
BssSI C'ACGA_G
Cuts at: 1052 2779 3137 1052
Size: 1727 358 2423
Fragments arranged by size:
2423 1727 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1335 1885 1335
Size: 550 3958
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstYI r'GATC_y
Cuts at: 1 996 1533 2000 2023 2358 2604 3616 3702
Size: 995 537 467 23 335 246 1012 86
Cuts at: 3702 3714 4060 1
Size: 12 346 449
Fragments arranged by size:
1012 995 537 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1137 1261 1137
Size: 124 4384
BtgI C'CryG_G
Cuts at: 107 107
Size: 4508
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 1751 1751
Size: 4508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 471 506 471
Size: 35 4473
DraI TTT'AAA
Cuts at: 240 1507 240
Size: 1267 3241
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 2093 2267 2658 2685 4030 2093
Size: 174 391 27 1345 2571
Fragments arranged by size:
2571 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 680 766 2531 2741 680
Size: 86 1765 210 2447
Fragments arranged by size:
2447 1765 210 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 568 649 723 730 790 826 2333 2765
Size: 57 81 74 7 60 36 1507 432
Cuts at: 2765 3512 3780 511
Size: 747 268 1239
Fragments arranged by size:
1507 1239 747 432 268 81 74 60
57 36 7
EcoNI CCTnn'n_nnAGG
Cuts at: 679 1493 679
Size: 814 3694
EcoO109I rG'GnC_Cy
Cuts at: 1647 1647
Size: 4508
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 963 1446 2527 963
Size: 483 1081 2944
Fragments arranged by size:
2944 1081 483
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 450 1942 2190 3212 4191 450
Size: 1492 248 1022 979 767
Fragments arranged by size:
1492 1022 979 767 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 626 658 981 1013 1228 1260 2350 2380 2382
Size: 32 323 32 215 32 1090 30 2
Cuts at: 2382 2412 626
Size: 30 2722
Fragments arranged by size:
2722 1090 323 215 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
HindIII A'AGCT_T
Cuts at: 940 940
Size: 4508
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 3179 3363 345
Size: 2834 184 1490
Fragments arranged by size:
2834 1490 184
MscI TGG'CCA
Cuts at: 2269 2269
Size: 4508
MslI CAynn'nnrTG
Cuts at: 592 1225 1322 2497 2624 592
Size: 633 97 1175 127 2476
Fragments arranged by size:
2476 1175 633 127 97
MspA1I CmG'CkG
Cuts at: 336 1619 1933 2293 3306 3551 336
Size: 1283 314 360 1013 245 1293
Fragments arranged by size:
1293 1283 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 107 107
Size: 4508
NdeI CA'TA_TG
Cuts at: 308 308
Size: 4508
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 1561 2061 2968 1561
Size: 500 907 3101
Fragments arranged by size:
3101 907 500
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 798 798
Size: 4508
PfoI T'CCnGG_A
Cuts at: 797 1087 797
Size: 290 4218
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1010 1042 3647 3679 1010
Size: 32 2605 32 1839
Fragments arranged by size:
2605 1839 32 32
PsiI TTA'TAA
Cuts at: 1385 1385
Size: 4508
PstI C_TGCA'G
Cuts at: 845 1555 2240 845
Size: 710 685 3113
Fragments arranged by size:
3113 710 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 336 1933 2293 336
Size: 1597 360 2551
Fragments arranged by size:
2551 1597 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 766 2531 2741 766
Size: 1765 210 2533
Fragments arranged by size:
2533 1765 210
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 841 1551 2236 3229 3420 33
Size: 808 710 685 993 191 1121
Fragments arranged by size:
1121 993 808 710 685 191
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1530 1530
Size: 4508
SmlI C'TyrA_G
Cuts at: 784 1663 2578 3070 3332 784
Size: 879 915 492 262 1960
Fragments arranged by size:
1960 915 879 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
SspI AAT'ATT
Cuts at: 1471 1471
Size: 4508
StyI C'CwwG_G
Cuts at: 107 908 1642 1996 107
Size: 801 734 354 2619
Fragments arranged by size:
2619 801 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1078 2091 2224 2683 2892 1078
Size: 1013 133 459 209 2694
Fragments arranged by size:
2694 1013 459 209 133
TatI w'GTAC_w
Cuts at: 4023 4023
Size: 4508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4025 4057 4025
Size: 32 4476
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 135 333 2003 135
Size: 198 1670 2640
Fragments arranged by size:
2640 1670 198
XmaI C'CCGG_G
Cuts at: 1528 1528
Size: 4508
XmnI GAAnn'nnTTC
Cuts at: 1022 1022
Size: 4508
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI