Restriction map for
pF1KB0986
(Circular) (Six-base) MAPSORT of: pF1KB0986.seq Check: 9801 from: 1 to: 4475
pF1KB0986 4475 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AatII G_ACGT'C
Cuts at: 374 374
Size: 4475
AccI GT'mk_AC
Cuts at: 265 1513 265
Size: 1248 3227
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 610 1195 1219 2300 2732 3479 3747 535
Size: 75 585 24 1081 432 747 268 1263
Fragments arranged by size:
1263 1081 747 585 432 268 75 24
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AhdI GACnn_n'nnGTC
Cuts at: 1227 1227
Size: 4475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 973 1005 973
Size: 32 4443
AlwNI CAG_nnn'CTG
Cuts at: 816 975 1313 1780 3347 816
Size: 159 338 467 1567 1944
Fragments arranged by size:
1944 1567 467 338 159
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 1479 3828 4084 1479
Size: 2349 256 1870
Fragments arranged by size:
2349 1870 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 543 1495 543
Size: 952 3523
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 570 829 930 1491 1905 2153 2188 570
Size: 259 101 561 414 248 35 2857
Fragments arranged by size:
2857 561 414 259 248 101 35
BanII G_rGCy'C
Cuts at: 544 782 965 1343 1489 2519 544
Size: 238 183 378 146 1030 2500
Fragments arranged by size:
2500 1030 378 238 183 146
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 220 220
Size: 4475
BbvCI CC'TCA_GC
Cuts at: 1051 1051
Size: 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 328 362 90
Size: 34 204 34 4203
Fragments arranged by size:
4203 204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3134 4475 2373
Size: 761 1341 2373
Fragments arranged by size:
2373 1341 761
BclI T'GATC_A
Cuts at: 486 627 1995 486
Size: 141 1368 2966
Fragments arranged by size:
2966 1368 141
BglI GCCn_nnn'nGGC
Cuts at: 112 868 112
Size: 756 3719
BglII A'GATC_T
Cuts at: 186 1391 1990 4027 186
Size: 1205 599 2037 634
Fragments arranged by size:
2037 1205 634 599
BlpI GC'TnA_GC
Cuts at: 203 733 1587 203
Size: 530 854 3091
Fragments arranged by size:
3091 854 530
Bme1580I G_kGCm'C
Cuts at: 2100 2193 3249 2100
Size: 93 1056 3326
Fragments arranged by size:
3326 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1284 1687 1768 2103 4247 1284
Size: 403 81 335 2144 1512
Fragments arranged by size:
2144 1512 403 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 478 760 826 1236 1298 1349 163
Size: 315 282 66 410 62 51 3289
Fragments arranged by size:
3289 410 315 282 66 62 51
Bpu10I CC'TnA_GC
Cuts at: 1051 1051
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1651 2530 3022 3320 1009
Size: 642 879 492 298 2164
Fragments arranged by size:
2164 879 642 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 4179 1087
Size: 3092 1383
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 371 1906 2154 2856 4352 371
Size: 1535 248 702 1496 494
Fragments arranged by size:
1535 1496 702 494 248
BsaWI w'CCGG_w
Cuts at: 1664 2185 2863 3137 3284 3695 1664
Size: 521 678 274 147 411 2444
Fragments arranged by size:
2444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 1347 1377 502
Size: 30 815 30 3600
Fragments arranged by size:
3600 815 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 523 781 1105 1449 145
Size: 378 258 324 344 3171
Fragments arranged by size:
3171 378 344 324 258
BseYI C'CCAG_C
Cuts at: 574 728 816 964 1313 1900 2658 3235 3731
Size: 154 88 148 349 587 758 577 496
Cuts at: 3731 574
Size: 1318
Fragments arranged by size:
1318 758 587 577 496 349 154 148
88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1264 1129
Size: 135 4340
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 698 843 1489 2267 2457 3249 698
Size: 145 646 778 190 792 1924
Fragments arranged by size:
1924 792 778 646 190 145
BsmI GAATG_Cn'
Cuts at: 472 472
Size: 4475
BsmBI CGTCTCn'nnnn_
Cuts at: 1036 1036
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 544 698 782 843 965 1343 1489 2100 2193
Size: 154 84 61 122 378 146 611 93
Cuts at: 2193 2267 2457 2519 3249 544
Size: 74 190 62 730 1770
Fragments arranged by size:
1770 730 611 378 190 154 146 122
93 84 74 62 61
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1105 1525 1739 2041 2422 1105
Size: 420 214 302 381 3158
Fragments arranged by size:
3158 420 381 302 214
BsrBI CCG'CTC
Cuts at: 50 744 2767 2821 4305 4470 50
Size: 694 2023 54 1484 165 55
Fragments arranged by size:
2023 1484 694 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 567 2473 2654 2863 567
Size: 1906 181 209 2179
Fragments arranged by size:
2179 1906 209 181
BssSI C'ACGA_G
Cuts at: 781 1087 2746 3104 781
Size: 306 1659 358 2152
Fragments arranged by size:
2152 1659 358 306
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 186 1391 1500 1967 1990 2325 2571 3583
Size: 185 1205 109 467 23 335 246 1012
Cuts at: 3583 3669 3681 4027 1
Size: 86 12 346 449
Fragments arranged by size:
1205 1012 467 449 346 335 246 185
109 86 23 12
BstZ17I GTA'TAC
Cuts at: 266 266
Size: 4475
BtgI C'CryG_G
Cuts at: 157 157
Size: 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1044 1718 1044
Size: 674 3801
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DraIII CAC_nnn'GTG
Cuts at: 621 1152 621
Size: 531 3944
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 525 2060 2234 2625 2652 3997 525
Size: 1535 174 391 27 1345 1003
Fragments arranged by size:
1535 1345 1003 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 677 977 1184 2498 2708 677
Size: 300 207 1314 210 2444
Fragments arranged by size:
2444 1314 300 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 598 2683 2993 3139 4285 130
Size: 468 2085 310 146 1146 320
Fragments arranged by size:
2085 1146 468 320 310 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 478 535 610 760 826 1195 1219 1236
Size: 315 57 75 150 66 369 24 17
Cuts at: 1236 1298 1349 2300 2732 3479 3747 163
Size: 62 51 951 432 747 268 891
Fragments arranged by size:
951 891 747 432 369 315 268 150
75 66 62 57 51 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 127 1101 127
Size: 974 3501
EcoO109I rG'GnC_Cy
Cuts at: 174 1269 1614 174
Size: 1095 345 3035
Fragments arranged by size:
3035 1095 345
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 337 1410 2494 337
Size: 1073 1084 2318
Fragments arranged by size:
2318 1084 1073
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 850 1172 1909 2157 3179 4158 850
Size: 322 737 248 1022 979 1167
Fragments arranged by size:
1167 1022 979 737 322 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1213 1245 2317 2347 2349 2379 502
Size: 32 679 32 1072 30 2 30 2598
Fragments arranged by size:
2598 1072 679 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1332 1514 1332
Size: 182 4293
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 3146 3330 221
Size: 2925 184 1366
Fragments arranged by size:
2925 1366 184
MscI TGG'CCA
Cuts at: 527 2236 527
Size: 1709 2766
MslI CAynn'nnrTG
Cuts at: 2464 2591 2464
Size: 127 4348
MspA1I CmG'CkG
Cuts at: 732 816 1310 1586 1900 2260 3273 3518 732
Size: 84 494 276 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 494 360 314 276 245 84
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 349 587 1528 2028 2935 349
Size: 238 941 500 907 1889
Fragments arranged by size:
1889 941 907 500 238
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 1313 1313
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 174 1269 174
Size: 1095 3380
PstI C_TGCA'G
Cuts at: 1263 1522 2207 1263
Size: 259 685 3531
Fragments arranged by size:
3531 685 259
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 732 816 1900 2260 732
Size: 84 1084 360 2947
Fragments arranged by size:
2947 1084 360 84
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SexAI A'CCwGG_T
Cuts at: 122 122
Size: 4475
SfcI C'TryA_G
Cuts at: 33 1259 1518 2203 3196 3387 33
Size: 1226 259 685 993 191 1121
Fragments arranged by size:
1226 1121 993 685 259 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 545 1497 545
Size: 952 3523
SmlI C'TyrA_G
Cuts at: 1024 1630 2545 3037 3299 1024
Size: 606 915 492 262 2200
Fragments arranged by size:
2200 915 606 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StuI AGG'CCT
Cuts at: 427 427
Size: 4475
StyI C'CwwG_G
Cuts at: 528 1112 1265 1609 1963 528
Size: 584 153 344 354 3040
Fragments arranged by size:
3040 584 354 344 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 588 2058 2191 2650 2859 588
Size: 1470 133 459 209 2204
Fragments arranged by size:
2204 1470 459 209 133
TatI w'GTAC_w
Cuts at: 1163 3990 1163
Size: 2827 1648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 3992 4024 142
Size: 32 3818 32 593
Fragments arranged by size:
3818 593 32 32
Tth111I GACn'n_nGTC
Cuts at: 2272 2272
Size: 4475
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 543 1495 543
Size: 952 3523
ZraI GAC'GTC
Cuts at: 372 372
Size: 4475
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII
BstXI Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI