Restriction map for
pF1KB0980
(Circular) (Six-base) MAPSORT of: pF1KB0980.seq Check: 7404 from: 1 to: 4463
pF1KB0980 4463 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 212 1501 212
Size: 1289 3174
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 774 2288 2720 3467 3735 774
Size: 1514 432 747 268 1502
Fragments arranged by size:
1514 1502 747 432 268
AflIII A'CryG_T
Cuts at: 1815 2919 3896 1815
Size: 1104 977 2382
Fragments arranged by size:
2382 1104 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AleI CACnn'nnGTG
Cuts at: 227 227
Size: 4463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 1768 3335 1768
Size: 1567 2896
ApaI G_GGCC'C
Cuts at: 1214 1214
Size: 4463
ApaLI G'TGCA_C
Cuts at: 1421 3233 1421
Size: 1812 2651
ApoI r'AATT_y
Cuts at: 1467 3816 4072 1467
Size: 2349 256 1858
Fragments arranged by size:
2349 1858 256
AseI AT'TA_AT
Cuts at: 20 491 20
Size: 471 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 805 814 1042 1483 805
Size: 9 228 441 3785
Fragments arranged by size:
3785 441 228 9
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 1479 1893 2141 2176 1479
Size: 414 248 35 3766
Fragments arranged by size:
3766 414 248 35
BanII G_rGCy'C
Cuts at: 343 640 662 1214 1477 2507 343
Size: 297 22 552 263 1030 2299
Fragments arranged by size:
2299 1030 552 297 263 22
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BbvCI CC'TCA_GC
Cuts at: 446 446
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4429
BciVI GTATCCnnnnn_n'
Cuts at: 1219 2361 3122 4463 1219
Size: 1142 761 1341 1219
Fragments arranged by size:
1341 1219 1142 761
BclI T'GATC_A
Cuts at: 711 1983 711
Size: 1272 3191
BglI GCCn_nnn'nGGC
Cuts at: 963 963
Size: 4463
BglII A'GATC_T
Cuts at: 127 1978 4015 127
Size: 1851 2037 575
Fragments arranged by size:
2037 1851 575
BlpI GC'TnA_GC
Cuts at: 594 674 1575 594
Size: 80 901 3482
Fragments arranged by size:
3482 901 80
Bme1580I G_kGCm'C
Cuts at: 476 823 1214 1425 2088 2181 3237 476
Size: 347 391 211 663 93 1056 1702
Fragments arranged by size:
1702 1056 663 391 347 211 93
BmrI ACTGGGnnnn_n'
Cuts at: 470 615 970 1675 1756 2091 4235 470
Size: 145 355 705 81 335 2144 698
Fragments arranged by size:
2144 705 698 355 335 145 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 454 2830 454
Size: 2376 2087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 1356 347
Size: 1009 3454
Bpu10I CC'TnA_GC
Cuts at: 446 446
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 2518 3010 3308 1639
Size: 879 492 298 2794
Fragments arranged by size:
2794 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 425 4167 425
Size: 3742 721
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 452 1894 2142 2844 4340 452
Size: 1442 248 702 1496 575
Fragments arranged by size:
1496 1442 702 575 248
BsaWI w'CCGG_w
Cuts at: 503 1652 2173 2851 3125 3272 3683 503
Size: 1149 521 678 274 147 411 1283
Fragments arranged by size:
1283 1149 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1177 1260 1329 1177
Size: 83 69 4311
Fragments arranged by size:
4311 83 69
BseYI C'CCAG_C
Cuts at: 153 589 862 1888 2646 3223 3719 153
Size: 436 273 1026 758 577 496 897
Fragments arranged by size:
1026 897 758 577 496 436 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4463
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3259 104
Size: 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1425 1477 2255 2445 3237 1425
Size: 52 778 190 792 2651
Fragments arranged by size:
2651 792 778 190 52
Bsp1286I G_dGCh'C
Cuts at: 343 476 640 662 823 1214 1425 1477 2088
Size: 133 164 22 161 391 211 52 611
Cuts at: 2088 2181 2255 2445 2507 3237 343
Size: 93 74 190 62 730 1569
Fragments arranged by size:
1569 730 611 391 211 190 164 161
133 93 74 62 52 22
BspEI T'CCGG_A
Cuts at: 503 3683 503
Size: 3180 1283
BspHI T'CATG_A
Cuts at: 3639 3639
Size: 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 1513 1727 2029 2410 287
Size: 1226 214 302 381 2340
Fragments arranged by size:
2340 1226 381 302 214
BsrBI CCG'CTC
Cuts at: 50 813 831 2755 2809 4293 4458 50
Size: 763 18 1924 54 1484 165 55
Fragments arranged by size:
1924 1484 763 165 55 54 18
BsrDI GCAATG_nn'
Cuts at: 1320 2375 1320
Size: 1055 3408
BsrFI r'CCGG_y
Cuts at: 434 2461 2642 2851 434
Size: 2027 181 209 2046
Fragments arranged by size:
2046 2027 209 181
BssSI C'ACGA_G
Cuts at: 2734 3092 2734
Size: 358 4105
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 372 372
Size: 4463
BstYI r'GATC_y
Cuts at: 1 127 287 425 1005 1098 1488 1955 1978
Size: 126 160 138 580 93 390 467 23
Cuts at: 1978 2313 2559 3571 3657 3669 4015 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 580 467 449 390 346 335 246
160 138 126 93 86 23 12
BtgI C'CryG_G
Cuts at: 135 270 995 1439 135
Size: 135 725 444 3159
Fragments arranged by size:
3159 725 444 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 654 1263 2413 2594 2757 654
Size: 609 1150 181 163 2360
Fragments arranged by size:
2360 1150 609 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1706 1433
Size: 273 4190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1428 1463 1428
Size: 35 4428
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 132 356 551 1323 2048 2222 2613 2640 3985
Size: 224 195 772 725 174 391 27 1345
Cuts at: 3985 132
Size: 610
Fragments arranged by size:
1345 772 725 610 391 224 195 174
27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 1077 2671 2981 3127 4273 187
Size: 890 1594 310 146 1146 377
Fragments arranged by size:
1594 1146 890 377 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 774 1356 2288 2720 3467 3735 347
Size: 427 582 932 432 747 268 1075
Fragments arranged by size:
1075 932 747 582 432 427 268
EcoNI CCTnn'n_nnAGG
Cuts at: 445 445
Size: 4463
EcoO109I rG'GnC_Cy
Cuts at: 161 236 885 1122 1182 1210 1602 161
Size: 75 649 237 60 28 392 3022
Fragments arranged by size:
3022 649 392 237 75 60 28
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1362 1897 2145 3167 4146 1362
Size: 535 248 1022 979 1679
Fragments arranged by size:
1679 1022 979 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 997 1029 2305 2335 2337 2367 997
Size: 32 1276 30 2 30 3093
Fragments arranged by size:
3093 1276 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 974 3134 3318 605
Size: 369 2160 184 1750
Fragments arranged by size:
2160 1750 369 184
MscI TGG'CCA
Cuts at: 134 358 553 1325 2224 134
Size: 224 195 772 899 2373
Fragments arranged by size:
2373 899 772 224 195
MslI CAynn'nnrTG
Cuts at: 227 2452 2579 227
Size: 2225 127 2111
Fragments arranged by size:
2225 2111 127
MspA1I CmG'CkG
Cuts at: 153 545 598 758 897 1250 1574 1888 2248
Size: 392 53 160 139 353 324 314 360
Cuts at: 2248 3261 3506 153
Size: 1013 245 1110
Fragments arranged by size:
1110 1013 392 360 353 324 314 245
160 139 53
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NcoI C'CATG_G
Cuts at: 135 270 135
Size: 135 4328
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 1516 2016 2923 1516
Size: 500 907 3056
Fragments arranged by size:
3056 907 500
PasI CC'CwG_GG
Cuts at: 633 633
Size: 4463
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PfoI T'CCnGG_A
Cuts at: 570 570
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 1122 1122
Size: 4463
PspOMI G'GGCC_C
Cuts at: 1210 1210
Size: 4463
PspXI vC'TCGA_Gb
Cuts at: 805 814 805
Size: 9 4454
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1153 1185 1153
Size: 32 4431
PstI C_TGCA'G
Cuts at: 1106 1510 2195 1106
Size: 404 685 3374
Fragments arranged by size:
3374 685 404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 153 545 598 1888 2248 153
Size: 392 53 1290 360 2368
Fragments arranged by size:
2368 1290 392 360 53
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SanDI GG'GwC_CC
Cuts at: 1122 1122
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 1102 1344 1506 2191 3184 3375 33
Size: 1069 242 162 685 993 191 1121
Fragments arranged by size:
1121 1069 993 685 242 191 162
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 805 814 1618 2533 3025 3287 805
Size: 9 804 915 492 262 1981
Fragments arranged by size:
1981 915 804 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StuI AGG'CCT
Cuts at: 850 1354 850
Size: 504 3959
StyI C'CwwG_G
Cuts at: 135 270 291 350 365 1597 1951 135
Size: 135 21 59 15 1232 354 2647
Fragments arranged by size:
2647 1232 354 135 59 21 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2046 2179 2638 2847 2046
Size: 133 459 209 3662
Fragments arranged by size:
3662 459 209 133
TatI w'GTAC_w
Cuts at: 3978 3978
Size: 4463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1223 1255 3980 4012 1223
Size: 32 2725 32 1674
Fragments arranged by size:
2725 1674 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 351 366 957 1284 1958 351
Size: 15 591 327 674 2856
Fragments arranged by size:
2856 674 591 327 15
XhoI C'TCGA_G
Cuts at: 805 814 805
Size: 9 4454
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI