Restriction map for
pF1KB0975
(Circular) (Six-base) MAPSORT of: pF1KB0975.seq Check: 7421 from: 1 to: 3746
pF1KB0975 3746 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 172 612 172
Size: 440 3306
AatII G_ACGT'C
Cuts at: 313 313
Size: 3746
AccI GT'mk_AC
Cuts at: 784 784
Size: 3746
Acc65I G'GTAC_C
Cuts at: 762 762
Size: 3746
AclI AA'CG_TT
Cuts at: 3586 3586
Size: 3746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1571 2003 2750 3018 667
Size: 904 432 747 268 1395
Fragments arranged by size:
1395 904 747 432 268
AflIII A'CryG_T
Cuts at: 1098 2202 3179 1098
Size: 1104 977 1665
Fragments arranged by size:
1665 1104 977
AgeI A'CCGG_T
Cuts at: 2134 2134
Size: 3746
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 508 540 508
Size: 32 3714
AlwNI CAG_nnn'CTG
Cuts at: 1051 2618 1051
Size: 1567 2179
ApaI G_GGCC'C
Cuts at: 640 640
Size: 3746
ApaLI G'TGCA_C
Cuts at: 320 400 2516 320
Size: 80 2116 1550
Fragments arranged by size:
2116 1550 80
ApoI r'AATT_y
Cuts at: 750 3099 3355 750
Size: 2349 256 1141
Fragments arranged by size:
2349 1141 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
AvaI C'yCGr_G
Cuts at: 337 616 766 337
Size: 279 150 3317
Fragments arranged by size:
3317 279 150
AvrII C'CTAG_G
Cuts at: 304 304
Size: 3746
BamHI G'GATC_C
Cuts at: 771 771
Size: 3746
BanI G'GyrC_C
Cuts at: 192 762 1176 1424 1459 192
Size: 570 414 248 35 2479
Fragments arranged by size:
2479 570 414 248 35
BanII G_rGCy'C
Cuts at: 640 760 1790 640
Size: 120 1030 2596
Fragments arranged by size:
2596 1030 120
BbeI G_GCGC'C
Cuts at: 1180 1428 1180
Size: 248 3498
BbsI GAAGACnn'nnnn_
Cuts at: 673 673
Size: 3746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3712
BciVI GTATCCnnnnn_n'
Cuts at: 1644 2405 3746 1644
Size: 761 1341 1644
Fragments arranged by size:
1644 1341 761
BclI T'GATC_A
Cuts at: 368 1266 368
Size: 898 2848
BglII A'GATC_T
Cuts at: 1261 3298 1261
Size: 2037 1709
BlpI GC'TnA_GC
Cuts at: 858 858
Size: 3746
Bme1580I G_kGCm'C
Cuts at: 324 404 640 1371 1464 2520 324
Size: 80 236 731 93 1056 1550
Fragments arranged by size:
1550 1056 731 236 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 958 1039 1374 3518 958
Size: 81 335 2144 1186
Fragments arranged by size:
2144 1186 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 3746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 541
Size: 3746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1801 2293 2591 922
Size: 879 492 298 2077
Fragments arranged by size:
2077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3450 3450
Size: 3746
BsaAI yAC'GTr
Cuts at: 1099 1099
Size: 3746
BsaBI GATnn'nnATC
Cuts at: 6 1260 3297 6
Size: 1254 2037 455
Fragments arranged by size:
2037 1254 455
BsaHI Gr'CG_yC
Cuts at: 310 1177 1425 2127 3623 310
Size: 867 248 702 1496 433
Fragments arranged by size:
1496 867 702 433 248
BsaWI w'CCGG_w
Cuts at: 935 1456 2134 2408 2555 2966 935
Size: 521 678 274 147 411 1715
Fragments arranged by size:
1715 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 508
Size: 30 3716
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 642 246
Size: 396 3350
BseYI C'CCAG_C
Cuts at: 1171 1929 2506 3002 1171
Size: 758 577 496 1915
Fragments arranged by size:
1915 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 508 206
Size: 302 3444
BsiEI CG_ry'CG
Cuts at: 104 1288 1334 2542 104
Size: 1184 46 1208 1308
Fragments arranged by size:
1308 1208 1184 46
BsiHKAI G_wGCw'C
Cuts at: 283 324 404 634 760 1538 1728 2520 283
Size: 41 80 230 126 778 190 792 1509
Fragments arranged by size:
1509 792 778 230 190 126 80 41
BsmBI CGTCTCn'nnnn_
Cuts at: 317 544 317
Size: 227 3519
Bsp1286I G_dGCh'C
Cuts at: 283 324 404 634 640 760 1371 1464 1538
Size: 41 80 230 6 120 611 93 74
Cuts at: 1538 1728 1790 2520 283
Size: 190 62 730 1509
Fragments arranged by size:
1509 730 611 230 190 120 93 80
74 62 41 6
BspEI T'CCGG_A
Cuts at: 2966 2966
Size: 3746
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 172 612 796 1010 1312 1693 172
Size: 440 184 214 302 381 2225
Fragments arranged by size:
2225 440 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 2038 2092 3576 3741 50
Size: 1988 54 1484 165 55
Fragments arranged by size:
1988 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1658 1658
Size: 3746
BsrFI r'CCGG_y
Cuts at: 198 1744 1925 2134 198
Size: 1546 181 209 1810
Fragments arranged by size:
1810 1546 209 181
BssSI C'ACGA_G
Cuts at: 2017 2375 2017
Size: 358 3388
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 1123
Size: 3746
BstBI TT'CG_AA
Cuts at: 2107 2107
Size: 3746
BstYI r'GATC_y
Cuts at: 1 771 1238 1261 1596 1842 2854 2940 2952
Size: 770 467 23 335 246 1012 86 12
Cuts at: 2952 3298 1
Size: 346 449
Fragments arranged by size:
1012 770 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 422 448 1696 1877 2040 422
Size: 26 1248 181 163 2128
Fragments arranged by size:
2128 1248 181 163 26
BtsI GCAGTG_nn'
Cuts at: 989 989
Size: 3746
DraI TTT'AAA
Cuts at: 745 745
Size: 3746
DraIII CAC_nnn'GTG
Cuts at: 544 544
Size: 3746
DrdI GACnn_nn'nnGTC
Cuts at: 1452 2310 1452
Size: 858 2888
EaeI y'GGCC_r
Cuts at: 1331 1505 1896 1923 3268 1331
Size: 174 391 27 1345 1809
Fragments arranged by size:
1809 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1331 1331
Size: 3746
EarI CTCTTCn'nnn_
Cuts at: 728 1769 1979 728
Size: 1041 210 2495
Fragments arranged by size:
2495 1041 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 173 1954 2264 2410 3556 173
Size: 1781 310 146 1146 363
Fragments arranged by size:
1781 1146 363 310 146
EcoICRI GAG'CTC
Cuts at: 758 758
Size: 3746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 667 1571 2003 2750 3018 541
Size: 126 904 432 747 268 1269
Fragments arranged by size:
1269 904 747 432 268 126
EcoNI CCTnn'n_nnAGG
Cuts at: 303 449 303
Size: 146 3600
EcoO109I rG'GnC_Cy
Cuts at: 225 885 225
Size: 660 3086
EcoRI G'AATT_C
Cuts at: 750 750
Size: 3746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1765 1765
Size: 3746
FspI TGC'GCA
Cuts at: 1527 1527
Size: 3746
HaeII r_GCGC'y
Cuts at: 1180 1428 2450 3429 1180
Size: 248 1022 979 1497
Fragments arranged by size:
1497 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1588 1618 1620 1650 1588
Size: 30 2 30 3684
Fragments arranged by size:
3684 30 30 2
HincII GTy'rAC
Cuts at: 785 785
Size: 3746
KasI G'GCGC_C
Cuts at: 1176 1424 1176
Size: 248 3498
KpnI G_GTAC'C
Cuts at: 766 766
Size: 3746
MluI A'CGCG_T
Cuts at: 3179 3179
Size: 3746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2601 2417
Size: 184 3562
MscI TGG'CCA
Cuts at: 1507 1507
Size: 3746
MslI CAynn'nnrTG
Cuts at: 706 1735 1862 706
Size: 1029 127 2590
Fragments arranged by size:
2590 1029 127
MspA1I CmG'CkG
Cuts at: 397 857 1171 1531 2544 2789 397
Size: 460 314 360 1013 245 1354
Fragments arranged by size:
1354 1013 460 360 314 245
NaeI GCC'GGC
Cuts at: 200 1927 200
Size: 1727 2019
NarI GG'CG_CC
Cuts at: 1177 1425 1177
Size: 248 3498
NgoMIV G'CCGG_C
Cuts at: 198 1925 198
Size: 1727 2019
NspI r_CATG'y
Cuts at: 799 1299 2206 799
Size: 500 907 2339
Fragments arranged by size:
2339 907 500
PciI A'CATG_T
Cuts at: 2202 2202
Size: 3746
PflMI CCAn_nnn'nTGG
Cuts at: 154 154
Size: 3746
PmeI GTTT'AAAC
Cuts at: 745 745
Size: 3746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 508 540 2885 2917 508
Size: 32 2345 32 1337
Fragments arranged by size:
2345 1337 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 225
Size: 3746
PspOMI G'GGCC_C
Cuts at: 636 636
Size: 3746
PstI C_TGCA'G
Cuts at: 793 1478 793
Size: 685 3061
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3746
PvuII CAG'CTG
Cuts at: 1171 1531 1171
Size: 360 3386
RsrII CG'GwC_CG
Cuts at: 1941 1941
Size: 3746
SacI G_AGCT'C
Cuts at: 760 760
Size: 3746
SalI G'TCGA_C
Cuts at: 783 783
Size: 3746
SanDI GG'GwC_CC
Cuts at: 225 225
Size: 3746
SapI GCTCTTCn'nnn_
Cuts at: 728 1769 1979 728
Size: 1041 210 2495
Fragments arranged by size:
2495 1041 210
SbfI CC_TGCA'GG
Cuts at: 793 793
Size: 3746
ScaI AGT'ACT
Cuts at: 3263 3263
Size: 3746
SfcI C'TryA_G
Cuts at: 33 420 789 1474 2467 2658 33
Size: 387 369 685 993 191 1121
Fragments arranged by size:
1121 993 685 387 369 191
SfoI GGC'GCC
Cuts at: 1178 1426 1178
Size: 248 3498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3746
SgrAI Cr'CCGG_yG
Cuts at: 198 198
Size: 3746
SmaI CCC'GGG
Cuts at: 618 768 618
Size: 150 3596
SmlI C'TyrA_G
Cuts at: 901 1816 2308 2570 901
Size: 915 492 262 2077
Fragments arranged by size:
2077 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3746
SphI G_CATG'C
Cuts at: 799 1299 799
Size: 500 3246
StyI C'CwwG_G
Cuts at: 153 304 880 1234 153
Size: 151 576 354 2665
Fragments arranged by size:
2665 576 354 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1329 1462 1921 2130 1329
Size: 133 459 209 2945
Fragments arranged by size:
2945 459 209 133
TatI w'GTAC_w
Cuts at: 3261 3261
Size: 3746
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 3714
Tth111I GACn'n_nGTC
Cuts at: 1543 1543
Size: 3746
XbaI T'CTAG_A
Cuts at: 447 777 447
Size: 330 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1241
Size: 3746
XmaI C'CCGG_G
Cuts at: 616 766 616
Size: 150 3596
ZraI GAC'GTC
Cuts at: 311 311
Size: 3746
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AscI BaeI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI