Restriction map for
pF1KB0974
(Circular) (Six-base) MAPSORT of: pF1KB0974.seq Check: 5528 from: 1 to: 4424
pF1KB0974 4424 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 822 822
Size: 4424
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2681 3428 3696 2249
Size: 432 747 268 2977
Fragments arranged by size:
2977 747 432 268
AfeI AGC'GCT
Cuts at: 1388 1388
Size: 4424
AflIII A'CryG_T
Cuts at: 278 1776 2880 3857 278
Size: 1498 1104 977 845
Fragments arranged by size:
1498 1104 977 845
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AleI CACnn'nnGTG
Cuts at: 412 412
Size: 4424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1045 1325 1045
Size: 280 4144
AlwNI CAG_nnn'CTG
Cuts at: 171 1729 3296 171
Size: 1558 1567 1299
Fragments arranged by size:
1567 1558 1299
ApaI G_GGCC'C
Cuts at: 385 471 695 385
Size: 86 224 4114
Fragments arranged by size:
4114 224 86
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 921 1428 3777 4033 921
Size: 507 2349 256 1312
Fragments arranged by size:
2349 1312 507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 1444 1444
Size: 4424
BamHI G'GATC_C
Cuts at: 493 1449 493
Size: 956 3468
BanI G'GyrC_C
Cuts at: 269 503 1147 1440 1854 2102 2137 269
Size: 234 644 293 414 248 35 2556
Fragments arranged by size:
2556 644 414 293 248 234 35
BanII G_rGCy'C
Cuts at: 359 385 471 695 918 1438 2468 359
Size: 26 86 224 223 520 1030 2315
Fragments arranged by size:
2315 1030 520 224 223 86 26
BbeI G_GCGC'C
Cuts at: 1151 1858 2106 1151
Size: 707 248 3469
Fragments arranged by size:
3469 707 248
BbsI GAAGACnn'nnnn_
Cuts at: 937 937
Size: 4424
BbvCI CC'TCA_GC
Cuts at: 1337 1337
Size: 4424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 460 1118 1152 426
Size: 34 658 34 3698
Fragments arranged by size:
3698 658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BfrBI ATG'CAT
Cuts at: 644 644
Size: 4424
BglI GCCn_nnn'nGGC
Cuts at: 1145 1145
Size: 4424
BglII A'GATC_T
Cuts at: 554 1939 3976 554
Size: 1385 2037 1002
Fragments arranged by size:
2037 1385 1002
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 385 471 508 695 2049 2142 3198 385
Size: 86 37 187 1354 93 1056 1611
Fragments arranged by size:
1611 1354 1056 187 93 86 37
BmrI ACTGGGnnnn_n'
Cuts at: 502 1636 1717 2052 4196 502
Size: 1134 81 335 2144 730
Fragments arranged by size:
2144 1134 730 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 4424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1366 556
Size: 810 3614
Bpu10I CC'TnA_GC
Cuts at: 601 1337 601
Size: 736 3688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 764 1034 1600 2479 2971 3269 519
Size: 245 270 566 879 492 298 1674
Fragments arranged by size:
1674 879 566 492 298 270 245
BsaI GGTCTCn'nnnn_
Cuts at: 1064 4128 1064
Size: 3064 1360
BsaAI yAC'GTr
Cuts at: 1777 1777
Size: 4424
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 1148 1855 2103 2805 4301 1148
Size: 707 248 702 1496 1271
Fragments arranged by size:
1496 1271 707 702 248
BsaWI w'CCGG_w
Cuts at: 374 1613 2134 2812 3086 3233 3644 374
Size: 1239 521 678 274 147 411 1154
Fragments arranged by size:
1239 1154 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 973 1003 973
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 583 623 981 1037 583
Size: 40 358 56 3970
Fragments arranged by size:
3970 358 56 40
BseYI C'CCAG_C
Cuts at: 480 650 1383 1849 2607 3184 3680 480
Size: 170 733 466 758 577 496 1224
Fragments arranged by size:
1224 758 733 577 496 466 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 318 1114 1321 184
Size: 134 796 207 3287
Fragments arranged by size:
3287 796 207 134
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 323 374 1438 2216 2406 3198 323
Size: 51 1064 778 190 792 1549
Fragments arranged by size:
1549 1064 792 778 190 51
BsmI GAATG_Cn'
Cuts at: 1211 1211
Size: 4424
BsmBI CGTCTCn'nnnn_
Cuts at: 838 838
Size: 4424
Bsp1286I G_dGCh'C
Cuts at: 323 359 374 385 471 508 695 918 1438
Size: 36 15 11 86 37 187 223 520
Cuts at: 1438 2049 2142 2216 2406 2468 3198 323
Size: 611 93 74 190 62 730 1549
Fragments arranged by size:
1549 730 611 520 223 190 187 93
86 74 62 37 36 15 11
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 3600 3600
Size: 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 674 822 1144 1474 1688 1990 2371 227
Size: 447 148 322 330 214 302 381 2280
Fragments arranged by size:
2280 447 381 330 322 302 214 148
BsrBI CCG'CTC
Cuts at: 50 299 2716 2770 4254 4419 50
Size: 249 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 249 165 55 54
BsrDI GCAATG_nn'
Cuts at: 235 2336 235
Size: 2101 2323
BsrFI r'CCGG_y
Cuts at: 1073 2422 2603 2812 1073
Size: 1349 181 209 2685
Fragments arranged by size:
2685 1349 209 181
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 171 1801 171
Size: 1630 2794
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 1391 1391
Size: 4424
BstYI r'GATC_y
Cuts at: 1 493 554 1271 1449 1916 1939 2274 2520
Size: 492 61 717 178 467 23 335 246
Cuts at: 2520 3532 3618 3630 3976 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 717 492 467 449 346 335 246
178 86 61 23 12
Bsu36I CC'TnA_GG
Cuts at: 595 595
Size: 4424
BtgI C'CryG_G
Cuts at: 695 695
Size: 4424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2374 2555 2718 2374
Size: 181 163 4080
Fragments arranged by size:
4080 181 163
BtsI GCAGTG_nn'
Cuts at: 151 1084 1667 151
Size: 933 583 2908
Fragments arranged by size:
2908 933 583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 866 901 866
Size: 35 4389
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 2130 2988 2130
Size: 858 3566
EaeI y'GGCC_r
Cuts at: 2009 2183 2574 2601 3946 2009
Size: 174 391 27 1345 2487
Fragments arranged by size:
2487 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 545 2447 2657 545
Size: 1902 210 2312
Fragments arranged by size:
2312 1902 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 2942 3088 4234 2632
Size: 310 146 1146 2822
Fragments arranged by size:
2822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1366 2249 2681 3428 3696 556
Size: 810 883 432 747 268 1284
Fragments arranged by size:
1284 883 810 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1064 1140 1064
Size: 76 4348
EcoO109I rG'GnC_Cy
Cuts at: 382 468 1341 1563 382
Size: 86 873 222 3243
Fragments arranged by size:
3243 873 222 86
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 172 1151 1390 1858 2106 3128 4107 172
Size: 979 239 468 248 1022 979 489
Fragments arranged by size:
1022 979 979 489 468 248 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 599 631 726 758 2266 2296 2298
Size: 32 89 32 95 32 1508 30 2
Cuts at: 2298 2328 478
Size: 30 2574
Fragments arranged by size:
2574 1508 95 89 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 450 1463 450
Size: 1013 3411
HpaI GTT'AAC
Cuts at: 450 450
Size: 4424
KasI G'GCGC_C
Cuts at: 1147 1854 2102 1147
Size: 707 248 3469
Fragments arranged by size:
3469 707 248
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 239 3095 3279 239
Size: 2856 184 1384
Fragments arranged by size:
2856 1384 184
MscI TGG'CCA
Cuts at: 2185 2185
Size: 4424
MslI CAynn'nnrTG
Cuts at: 283 412 581 757 1160 1204 2413 2540 283
Size: 129 169 176 403 44 1209 127 2167
Fragments arranged by size:
2167 1209 403 176 169 129 127 44
MspA1I CmG'CkG
Cuts at: 251 260 1535 1849 2209 3222 3467 251
Size: 9 1275 314 360 1013 245 1208
Fragments arranged by size:
1275 1208 1013 360 314 245 9
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 4424
NarI GG'CG_CC
Cuts at: 1148 1855 2103 1148
Size: 707 248 3469
Fragments arranged by size:
3469 707 248
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 4424
NsiI A_TGCA'T
Cuts at: 646 646
Size: 4424
NspI r_CATG'y
Cuts at: 202 648 1477 1977 2884 202
Size: 446 829 500 907 1742
Fragments arranged by size:
1742 907 829 500 446
PasI CC'CwG_GG
Cuts at: 1142 1142
Size: 4424
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PflMI CCAn_nnn'nTGG
Cuts at: 794 794
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PspOMI G'GGCC_C
Cuts at: 381 467 691 381
Size: 86 224 4114
Fragments arranged by size:
4114 224 86
PstI C_TGCA'G
Cuts at: 904 1471 2156 904
Size: 567 685 3172
Fragments arranged by size:
3172 685 567
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 251 1849 2209 251
Size: 1598 360 2466
Fragments arranged by size:
2466 1598 360
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 900 1467 2152 3145 3336 33
Size: 867 567 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 567 191
SfoI GGC'GCC
Cuts at: 1149 1856 2104 1149
Size: 707 248 3469
Fragments arranged by size:
3469 707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 1446 1446
Size: 4424
SmlI C'TyrA_G
Cuts at: 498 779 1049 1579 2494 2986 3248 498
Size: 281 270 530 915 492 262 1674
Fragments arranged by size:
1674 915 530 492 281 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 648 1477 1977 648
Size: 829 500 3095
Fragments arranged by size:
3095 829 500
StyI C'CwwG_G
Cuts at: 326 344 563 1101 1558 1912 326
Size: 18 219 538 457 354 2838
Fragments arranged by size:
2838 538 457 354 219 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2007 2140 2599 2808 2007
Size: 133 459 209 3623
Fragments arranged by size:
3623 459 209 133
TatI w'GTAC_w
Cuts at: 3939 3939
Size: 4424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 293 325 973 1005 3941 3973 293
Size: 32 648 32 2936 32 744
Fragments arranged by size:
2936 744 648 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1919 1391
Size: 528 3896
XmaI C'CCGG_G
Cuts at: 1444 1444
Size: 4424
XmnI GAAnn'nnTTC
Cuts at: 1110 1110
Size: 4424
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HindIII MfeI NcoI NdeI NheI
NotI NruI PacI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI