Restriction map for
pF1KB0970
(Circular) (Six-base) MAPSORT of: pF1KB0970.seq Check: 8268 from: 1 to: 4406
pF1KB0970 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 1353 4246 1353
Size: 2893 1513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 2231 2663 3410 3678 848
Size: 1383 432 747 268 1576
Fragments arranged by size:
1576 1383 747 432 268
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AhdI GACnn_n'nnGTC
Cuts at: 231 231
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1711 3278 1711
Size: 1567 2839
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 492 1410 3759 4015 492
Size: 918 2349 256 883
Fragments arranged by size:
2349 918 883 256
AseI AT'TA_AT
Cuts at: 20 299 20
Size: 279 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 637 1431 637
Size: 794 3612
BanI G'GyrC_C
Cuts at: 1032 1422 1836 2084 2119 1032
Size: 390 414 248 35 3319
Fragments arranged by size:
3319 414 390 248 35
BanII G_rGCy'C
Cuts at: 269 1420 2450 269
Size: 1151 1030 2225
Fragments arranged by size:
2225 1151 1030
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 296 1142 296
Size: 846 3560
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 741 1376 1926 741
Size: 635 550 3221
Fragments arranged by size:
3221 635 550
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 687 2031 2124 3180 687
Size: 1344 93 1056 1913
Fragments arranged by size:
1913 1344 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1618 1699 2034 4178 1618
Size: 81 335 2144 1846
Fragments arranged by size:
2144 1846 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 673
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 1344 1582 2461 2953 3251 269
Size: 1075 238 879 492 298 1424
Fragments arranged by size:
1424 1075 879 492 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 894 924 894
Size: 30 4376
BseYI C'CCAG_C
Cuts at: 686 786 887 1831 2589 3166 3662 686
Size: 100 101 944 758 577 496 1430
Fragments arranged by size:
1430 944 758 577 496 101 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1109
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1420 2198 2388 3180 1420
Size: 778 190 792 2646
Fragments arranged by size:
2646 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 269 687 1420 2031 2124 2198 2388 2450 3180
Size: 418 733 611 93 74 190 62 730
Cuts at: 3180 269
Size: 1495
Fragments arranged by size:
1495 733 730 611 418 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1456 1670 1972 2353 1456
Size: 214 302 381 3509
Fragments arranged by size:
3509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 267 1001 2318 267
Size: 734 1317 2355
Fragments arranged by size:
2355 1317 734
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstYI r'GATC_y
Cuts at: 1 637 1385 1431 1898 1921 2256 2502 3514
Size: 636 748 46 467 23 335 246 1012
Cuts at: 3514 3600 3612 3958 1
Size: 86 12 346 449
Fragments arranged by size:
1012 748 636 467 449 346 335 246
86 46 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 1224 1649 1224
Size: 425 3981
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 537 899 1991 2165 2556 2583 3928 537
Size: 362 1092 174 391 27 1345 1015
Fragments arranged by size:
1345 1092 1015 391 362 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 848 2231 2663 3410 3678 673
Size: 175 1383 432 747 268 1401
Fragments arranged by size:
1401 1383 747 432 268 175
EcoO109I rG'GnC_Cy
Cuts at: 1271 1545 1271
Size: 274 4132
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
EcoRV GAT'ATC
Cuts at: 603 603
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 1840 2088 3110 4089 1840
Size: 248 1022 979 2157
Fragments arranged by size:
2157 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 463 495 2248 2278 2280 2310 217
Size: 32 214 32 1753 30 2 30 2313
Fragments arranged by size:
2313 1753 214 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1109 3077 3261 1109
Size: 1968 184 2254
Fragments arranged by size:
2254 1968 184
MscI TGG'CCA
Cuts at: 539 901 2167 539
Size: 362 1266 2778
Fragments arranged by size:
2778 1266 362
MslI CAynn'nnrTG
Cuts at: 2395 2522 2395
Size: 127 4279
MspA1I CmG'CkG
Cuts at: 897 1517 1831 2191 3204 3449 897
Size: 620 314 360 1013 245 1854
Fragments arranged by size:
1854 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 344 1459 1959 2866 344
Size: 1115 500 907 1884
Fragments arranged by size:
1884 1115 907 500
PasI CC'CwG_GG
Cuts at: 1212 1212
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PflMI CCAn_nnn'nTGG
Cuts at: 996 996
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PpuMI rG'GwC_Cy
Cuts at: 1271 1271
Size: 4406
PsiI TTA'TAA
Cuts at: 701 701
Size: 4406
PstI C_TGCA'G
Cuts at: 1453 2138 1453
Size: 685 3721
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 897 1831 2191 897
Size: 934 360 3112
Fragments arranged by size:
3112 934 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SanDI GG'GwC_CC
Cuts at: 1271 1271
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 959 1449 2134 3127 3318 33
Size: 926 490 685 993 191 1121
Fragments arranged by size:
1121 993 926 685 490 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 284 1359 1561 2476 2968 3230 284
Size: 1075 202 915 492 262 1460
Fragments arranged by size:
1460 1075 915 492 262 202
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StuI AGG'CCT
Cuts at: 1307 1307
Size: 4406
StyI C'CwwG_G
Cuts at: 1540 1894 1540
Size: 354 4052
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 3921 3921
Size: 4406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 894 926 3923 3955 894
Size: 32 2997 32 1345
Fragments arranged by size:
2997 1345 32 32
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 207 1437 207
Size: 1230 3176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1253 1901 1253
Size: 648 3758
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI