Restriction map for
pF1KB0968
(Circular) (Six-base) MAPSORT of: pF1KB0968.seq Check: 5896 from: 1 to: 4406
pF1KB0968 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 345 1180 345
Size: 835 3571
AccI GT'mk_AC
Cuts at: 482 1444 482
Size: 962 3444
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 2231 2663 3410 3678 1060
Size: 1171 432 747 268 1788
Fragments arranged by size:
1788 1171 747 432 268
AfeI AGC'GCT
Cuts at: 1192 1192
Size: 4406
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1150 1182 1150
Size: 32 4374
AlwNI CAG_nnn'CTG
Cuts at: 964 1711 3278 964
Size: 747 1567 2092
Fragments arranged by size:
2092 1567 747
ApaI G_GGCC'C
Cuts at: 413 767 413
Size: 354 4052
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 184 1410 3759 4015 184
Size: 1226 2349 256 575
Fragments arranged by size:
2349 1226 575 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1154 1248 1426 1154
Size: 94 178 4134
Fragments arranged by size:
4134 178 94
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 418 1422 1836 2084 2119 418
Size: 1004 414 248 35 2705
Fragments arranged by size:
2705 1004 414 248 35
BanII G_rGCy'C
Cuts at: 329 413 767 1420 2450 329
Size: 84 354 653 1030 2285
Fragments arranged by size:
2285 1030 653 354 84
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglI GCCn_nnn'nGGC
Cuts at: 450 450
Size: 4406
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 668 1518 668
Size: 850 3556
Bme1580I G_kGCm'C
Cuts at: 413 446 455 767 2031 2124 3180 413
Size: 33 9 312 1264 93 1056 1639
Fragments arranged by size:
1639 1264 1056 312 93 33 9
BmrI ACTGGGnnnn_n'
Cuts at: 470 878 1256 1618 1699 2034 4178 470
Size: 408 378 362 81 335 2144 698
Fragments arranged by size:
2144 698 408 378 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1130 2773 1130
Size: 1643 2763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 335 617 1150 1385 1414 132
Size: 203 282 533 235 29 3124
Fragments arranged by size:
3124 533 282 235 203 29
Bpu10I CC'TnA_GC
Cuts at: 1213 1376 1213
Size: 163 4243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1582 2461 2953 3251 145
Size: 1437 879 492 298 1300
Fragments arranged by size:
1437 1300 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 296 1920 3957 6
Size: 290 1624 2037 455
Fragments arranged by size:
2037 1624 455 290
BsaHI Gr'CG_yC
Cuts at: 112 1321 1837 2085 2787 4283 112
Size: 1209 516 248 702 1496 235
Fragments arranged by size:
1496 1209 702 516 248 235
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 558 588 1150 1180 130
Size: 30 398 30 562 30 3356
Fragments arranged by size:
3356 562 398 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 900 1321 142
Size: 758 421 3227
Fragments arranged by size:
3227 758 421
BseYI C'CCAG_C
Cuts at: 686 738 767 948 964 1278 1831 2589 3166
Size: 52 29 181 16 314 553 758 577
Cuts at: 3166 3662 686
Size: 496 1430
Fragments arranged by size:
1430 758 577 553 496 314 181 52
29 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 198
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1420 2198 2388 3180 1420
Size: 778 190 792 2646
Fragments arranged by size:
2646 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 329 413 446 455 767 1420 2031 2124 2198
Size: 84 33 9 312 653 611 93 74
Cuts at: 2198 2388 2450 3180 329
Size: 190 62 730 1555
Fragments arranged by size:
1555 730 653 611 312 190 93 84
74 62 33 9
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 1180 1456 1670 1972 2353 345
Size: 835 276 214 302 381 2398
Fragments arranged by size:
2398 835 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 945 2698 2752 4236 4401 50
Size: 895 1753 54 1484 165 55
Fragments arranged by size:
1753 1484 895 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2318 2318
Size: 4406
BsrFI r'CCGG_y
Cuts at: 2404 2585 2794 2404
Size: 181 209 4016
Fragments arranged by size:
4016 209 181
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 777 1783 777
Size: 1006 3400
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 156 285 430 634 807 1003 1278 156
Size: 129 145 204 173 196 275 3284
Fragments arranged by size:
3284 275 204 196 173 145 129
BstYI r'GATC_y
Cuts at: 1 1431 1898 1921 2256 2502 3514 3600 3612
Size: 1430 467 23 335 246 1012 86 12
Cuts at: 3612 3958 1
Size: 346 449
Fragments arranged by size:
1430 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 363 363
Size: 4406
BtgI C'CryG_G
Cuts at: 107 284 577 853 1233 107
Size: 177 293 276 380 3280
Fragments arranged by size:
3280 380 293 276 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 267 649 812 900 1649 267
Size: 382 163 88 749 3024
Fragments arranged by size:
3024 749 382 163 88
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 171 206 171
Size: 35 4371
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DraIII CAC_nnn'GTG
Cuts at: 859 859
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 158 1991 2165 2556 2583 3928 158
Size: 1833 174 391 27 1345 636
Fragments arranged by size:
1833 1345 636 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 465 1099 1298 2429 2639 465
Size: 634 199 1131 210 2232
Fragments arranged by size:
2232 1131 634 210 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 335 617 1060 1150 1385 1414 2231 2663
Size: 203 282 443 90 235 29 817 432
Cuts at: 2663 3410 3678 132
Size: 747 268 860
Fragments arranged by size:
860 817 747 443 432 282 268 235
203 90 29
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 4406
EcoO109I rG'GnC_Cy
Cuts at: 426 763 764 1073 1252 1545 426
Size: 337 1 309 179 293 3287
Fragments arranged by size:
3287 337 309 293 179 1
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1065 2425 1065
Size: 1360 3046
FspI TGC'GCA
Cuts at: 1144 2187 1144
Size: 1043 3363
FspAI rTGC'GCAy
Cuts at: 1144 1144
Size: 4406
HaeII r_GCGC'y
Cuts at: 909 1194 1840 2088 3110 4089 909
Size: 285 646 248 1022 979 1226
Fragments arranged by size:
1226 1022 979 646 285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2248 2278 2280 2310 2248
Size: 30 2 30 4344
Fragments arranged by size:
4344 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3077 3261 3077
Size: 184 4222
MscI TGG'CCA
Cuts at: 160 2167 160
Size: 2007 2399
MslI CAynn'nnrTG
Cuts at: 509 2395 2522 509
Size: 1886 127 2393
Fragments arranged by size:
2393 1886 127
MspA1I CmG'CkG
Cuts at: 690 961 992 1517 1831 2191 3204 3449 690
Size: 271 31 525 314 360 1013 245 1647
Fragments arranged by size:
1647 1013 525 360 314 271 245 31
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 107 284 577 107
Size: 177 293 3936
Fragments arranged by size:
3936 293 177
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 775 1459 1959 2866 775
Size: 684 500 907 2315
Fragments arranged by size:
2315 907 684 500
PasI CC'CwG_GG
Cuts at: 406 430 406
Size: 24 4382
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 3545 3577 1150
Size: 32 2363 32 1979
Fragments arranged by size:
2363 1979 32 32
PpuMI rG'GwC_Cy
Cuts at: 426 1252 426
Size: 826 3580
PspOMI G'GGCC_C
Cuts at: 409 763 409
Size: 354 4052
PstI C_TGCA'G
Cuts at: 342 1453 2138 342
Size: 1111 685 2610
Fragments arranged by size:
2610 1111 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 690 992 1831 2191 690
Size: 302 839 360 2905
Fragments arranged by size:
2905 839 360 302
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SanDI GG'GwC_CC
Cuts at: 1252 1252
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 465 1099 2429 2639 465
Size: 634 1330 210 2232
Fragments arranged by size:
2232 1330 634 210
SbfI CC_TGCA'GG
Cuts at: 342 1453 342
Size: 1111 3295
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 338 1449 2134 3127 3318 33
Size: 305 1111 685 993 191 1121
Fragments arranged by size:
1121 1111 993 685 305 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1156 1250 1428 1156
Size: 94 178 4134
Fragments arranged by size:
4134 178 94
SmlI C'TyrA_G
Cuts at: 124 1561 2476 2968 3230 124
Size: 1437 915 492 262 1300
Fragments arranged by size:
1437 1300 915 492 262
SpeI A'CTAG_T
Cuts at: 67 438 67
Size: 371 4035
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 107 284 446 577 800 1271 1540 1894 107
Size: 177 162 131 223 471 269 354 2619
Fragments arranged by size:
2619 471 354 269 223 177 162 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 372 436 1246 1989 2122 2581 2790 372
Size: 64 810 743 133 459 209 1988
Fragments arranged by size:
1988 810 743 459 209 133 64
TatI w'GTAC_w
Cuts at: 3921 3921
Size: 4406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 558 590 3923 3955 130
Size: 32 396 32 3333 32 581
Fragments arranged by size:
3333 581 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 403 1901 403
Size: 1498 2908
XmaI C'CCGG_G
Cuts at: 1154 1248 1426 1154
Size: 94 178 4134
Fragments arranged by size:
4134 178 94
XmnI GAAnn'nnTTC
Cuts at: 1165 1165
Size: 4406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI